Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"Pharmacological properties of Humulus scandens: Insights from the TCMSP database and existing understanding","authors":"De Xin Dang ,&nbsp;Desheng Li ,&nbsp;Shi Qi Xu ,&nbsp;Feng He ,&nbsp;Xinhua Xia ,&nbsp;Shaoyong Xu","doi":"10.1016/j.prmcm.2024.100518","DOIUrl":"10.1016/j.prmcm.2024.100518","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Humulus scandens</em> (HS) has been traditionally used in folk medicine for treating a wide spectrum of ailments. Despite its long history of use, its integration into modern medicine has been relatively limited. This review aims to summarize the existing understanding of the pharmacological effects of HS and explore its potential pharmacological benefits using the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database.</div></div><div><h3>Methods</h3><div>we reviewed the pharmacological effects of HS by conducting a thorough literature search across databases like Google Scholar, CNKI, RISS, and J-Stage, as well as searching classical sources such as “Ben Cao Gang Mu” and “Ming Yi Bie Lu”. Furthermore, we used the TCMSP database to identify bioactive components of HS, focusing on those with oral bioavailability ≥ 30 % and drug-likeness ≥ 0.18, and explored the potential diseases associated with these components.</div></div><div><h3>Results</h3><div>HS as a traditional herbal medicine has been used to treat congestion, hematuria, strangury, watery diarrhea, and sores, as well as to enhance kidney function and promote urination. It has also served as a remedy for snake and scorpion bites. Modern experimental evidence suggests that HS exhibits efficacy in treating a wide range of conditions, including inflammation, and disorders of the respiratory system, urinary system, skin, gastrointestinal tract, nervous system, and cardiocerebral vascular system, as well as immunity-related diseases. Furthermore, it demonstrates promising properties in anti-cancer activity, hepatoprotection, lipid metabolism regulation, pain relief, alleviating diarrhea, and treating mental illness. Beyond these known effects, evidence from the TCMSP database highlights its potential in treating tumors, eye disorders, muscle disorders, diabetes, insomnia, and developmental disabilities.</div></div><div><h3>Discussion</h3><div>HS is a traditional herbal medicine with multiple pharmacological properties. However, while some pharmacological properties of HS have been validated through experiments, further research efforts are needed to explore its other effects. Such endeavors are crucial for a thorough understanding of HS and its potential therapeutic application.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100518"},"PeriodicalIF":0.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Wild Chaga (Inonotus obliquus) modulates inflammation, neural cell survival and inhibits proliferation of cancer cells” [Pharmacological Research - Modern Chinese Medicine 9C (2023) /100328]","authors":"Susan SC Cheung ,&nbsp;Garyen Chong ,&nbsp;Ingrid Elisia ,&nbsp;David Hasman ,&nbsp;Martin Lee ,&nbsp;Linda Chang ,&nbsp;Ziliang Ao ,&nbsp;Djamel Khelifi ,&nbsp;Gerald Krystal ,&nbsp;Joseph Tai","doi":"10.1016/j.prmcm.2024.100515","DOIUrl":"10.1016/j.prmcm.2024.100515","url":null,"abstract":"","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100515"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266714252400157X/pdfft?md5=3473fd105a21110d4dfd7501ee31da52&pid=1-s2.0-S266714252400157X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Syringic acid ameliorates testicular oxidative stress via the conservation of endogenous antioxidant markers and inhibition of the activated Nrf2-Keap1-NQO1-HO1 signaling in methyl cellosolve-administered rats” [Pharmacological Research - Modern Chinese Medicine 6C (2023) /100207]","authors":"Oluwatobi T. Somade ,&nbsp;Basiru O. Ajiboye ,&nbsp;Olukemi A. Osukoya ,&nbsp;Theophilus A. Jarikre ,&nbsp;Babatunji E. Oyinloye","doi":"10.1016/j.prmcm.2024.100513","DOIUrl":"10.1016/j.prmcm.2024.100513","url":null,"abstract":"","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100513"},"PeriodicalIF":0.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001556/pdfft?md5=82f9a3266a2c0ae7e5f1d98029845fe6&pid=1-s2.0-S2667142524001556-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Clinical efficacy and safety of Xuanfei Touxie Fang as a complementary medicine in the treatment of SARS-CoV-2 Omicron variant: A retrospective study” [Pharmacological Research - Modern Chinese Medicine 11C (2024) /100434]","authors":"Qingyuan Zhu,&nbsp;Baizhu Chen,&nbsp;Huixi Tong,&nbsp;Xiangyu Xu,&nbsp;Dongxiao Yang,&nbsp;Shuiyou Wang","doi":"10.1016/j.prmcm.2024.100516","DOIUrl":"10.1016/j.prmcm.2024.100516","url":null,"abstract":"","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100516"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001581/pdfft?md5=7983fa3dc547ee9998f34d757e636c35&pid=1-s2.0-S2667142524001581-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Drosophila model and network pharmacology to explore novel targets and novel active components of chinese traditional medications for treating kidney stones” [Pharmacological Research - Modern Chinese Medicine 6C (2023) /100220]","authors":"Shiyu Zhang ,&nbsp;Jingnan Zhu ,&nbsp;Yingjie Ju ,&nbsp;Mingxia Lv ,&nbsp;Rongdian Yang ,&nbsp;Ying Li ,&nbsp;Yaodong Miao ,&nbsp;Yiwen Wang","doi":"10.1016/j.prmcm.2024.100517","DOIUrl":"10.1016/j.prmcm.2024.100517","url":null,"abstract":"","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100517"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001593/pdfft?md5=d74da3e4564f073f5c8bdedfbf04e68b&pid=1-s2.0-S2667142524001593-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory activity and molecular docking studies of the hydromethanolic leaf extract of Baphia longipedicellata brumitt in rats","authors":"Farouk Adedeji Oladoja ,&nbsp;Olufunsho Awodele ,&nbsp;Ibrahim A. Oreagba ,&nbsp;Emmanuel S. Irokosu ,&nbsp;Elijah O. Oyinloye ,&nbsp;Akanji A. Murtala","doi":"10.1016/j.prmcm.2024.100512","DOIUrl":"10.1016/j.prmcm.2024.100512","url":null,"abstract":"<div><div><strong>Background</strong>: Almost all Baphia species of the Fabaceae family have a long history in traditional China due to their potent anti-inflammatory properties <em>Baphia longipedicellata</em> (<em>B. longipedicellata</em>) is a relatively novel plant comprised mainly of stigmasterol, which has a rich history in Chinese medicine and is a principal active component in numerous Chinese herbs such as <em>maidong</em>, the <em>banxia-baizhu-tianma decoction</em> and <em>Qingfengteng</em> which are all native to China and well known for its use in inflammatory diseases, asthma and diabetes management.</div><div><strong>Aim of the study:</strong> The study was meticulously designed to evaluate the anti-inflammatory activity of <em>Baphia longipedicellata</em> in rats. What sets this study apart is its unique approach to predicting the mechanism of anti-inflammatory action through docking studies.</div><div><strong>Materials and Methods:</strong> Preliminary phytochemical screening and oral acute toxicity were carried out for the hydromethanolic extract of <em>Baphia longipedicellata</em> (HMBL). Phytocompounds in the extract were identified using high-performance Liquid Chromatography (HPLC). Carrageenan and egg albumin-induced paw edema models in rats were evaluated to ascertain the extract's anti-inflammatory potential.</div><div>Molecular docking experiments were carried out to evaluate the binding affinity of HPLC-selected compounds to cyclooxygenase-2 (COX-2) and tumour necrosis Factor-alpha (TNF-alpha).</div><div><strong>Results and Discussion:</strong> Preliminary phytochemical screening revealed the presence of saponin, tannins, cardiac glycosides, alkaloids, terpenoids, etc. Results obtained from this study showed that the LD50 was greater than 2000 mg/kg, and there were no observable toxic effects or mortality following its acute exposure.</div><div>HPLC of HMBL profiled, Curcumin, Lutein, Stigmasterol, Beta-Sitosterol, Chrysarobin, Quercetin, and Napabucasin,</div><div>HMBL extract at all treatment doses showed a significant reduction in both carrageenan and egg albumin-induced hind paw edema in a dose-dependent manner, comparable to the standard drug Diclofenac. Peak anti-oedematogenic activity was observed at the 6th-hour post-carrageenan injection with 28.50, 42.06, and 36.90 % inhibition for HMBL (100, 200, or 400 mg/kg) and 5th-hour post-egg-albumin injection with 61.22, 66.88, and 48.06 % inhibition.</div><div>The molecular docking study showed that all the isolated HPLC compounds inhibited COX-2 and TNF-alpha.</div><div><strong>Conclusion</strong> Ultimately, the hydro-methanolic leaf extract of <em>B. longipedicellata</em> exhibits potent anti-inflammatory activity via COX 2 and TNF-alpha inhibition. Thus, it would be a viable phytotherapeutic option for treating inflammatory conditions.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100512"},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological effects of Berberine – A Chinese medicine, against xenobiotics toxicity","authors":"Annu Phogat,&nbsp;Jagjeet Singh,&nbsp;Vinay Malik","doi":"10.1016/j.prmcm.2024.100507","DOIUrl":"10.1016/j.prmcm.2024.100507","url":null,"abstract":"<div><h3>Introduction</h3><div>Traditional Chinese medicine has used plant-derived natural antioxidants from herbs since ages. Berberine is an iso-quinoline-based alkaloid found in various medicinal herbs: <em>Coptis chinensis (Huanglian), Phellodendron amurense (Huangbai</em>) and used in traditional Chinese medicine as decoctions: ‘<em>Xie Xin</em>’ and ‘<em>Huanglian Jiedu</em>’. It has been used since long to treat inflammation, auto-immune diseases and dysentery. Studies have also confirmed its antioxidative, anti-inflammatory, anti-apoptotic, and pharmacological properties. Still, there is a gap between the preclinical significance and clinical efficacy of Berberine against organ toxicity. Thus, the present review summarises the ameliorative potential of Berberine against free radicals-mediated oxidative stress, cholinergic stress, inflammation, mitochondrial dysfunctions, and apoptosis.</div></div><div><h3>Methods</h3><div>Various scientific research articles in Google Scholar, PubMed, Scopus, Web of Science, and ScienceDirect, were explored to find the pharmacological effects of Berberine. More than 80 articles since 2010 were explored among which 40 were shortlisted with the keywords: Berberine followed by ‘mechanism,’ ‘ameliorative properties,’ ‘pharmacological properties,’ ‘antioxidant; Berberine against ‘pesticide toxicity’, ‘heavy metal toxicity’ ‘nephrotoxicity’ ‘hepatotoxicity’ ‘neurotoxicity’. The thus selected research articles were carefully read to summarise the latest and most recent developments in writing of this review.</div></div><div><h3>Results</h3><div>The structural and functional diversity of Berberine is responsible for the protective efficacy against organ toxicity. The molecular mechanism of Berberine suggests attenuation of oxidative stress, regulation of endogenous antioxidants, and activation of signaling pathways, including PPARɣ/PGC-1α, HNF4α/MTTP, PTEN/Akt, Nrf-2/HO-1/MnSOD, and SIRT1/P66Shc along with inhibition of NF-κB translocation; can ameliorate organ dysfunctioning.</div></div><div><h3>Discussion</h3><div>The current review summarises a baseline and preclinical evidence comprising their mechanistic bases, highlighting the ethnopharmacological applications of Berberine for both traditional Chinese medicine and modern therapeutics. This evaluation will give the crucial insight essential for toxicological and pharmacokinetic research on human models to verify the safety issues and confirm the effects of active components of Berberine in the body in a clinical context.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100507"},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001490/pdfft?md5=cceb8eedb132a15c4a47e76fb0bba748&pid=1-s2.0-S2667142524001490-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gamma Oryzanol: A natural compound with potential for treating polycystic ovary syndrome","authors":"Sayantika Chakraborty,&nbsp;Navneet Khurana,&nbsp;Jaskiran Kaur,&nbsp;Meenu Mehta,&nbsp;Neha Sharma","doi":"10.1016/j.prmcm.2024.100506","DOIUrl":"10.1016/j.prmcm.2024.100506","url":null,"abstract":"<div><h3>Introduction</h3><p>Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age. Current management strategies only provide symptomatic relief, and there is a need for better treatments. Gamma-oryzanol, a dietary component found in rice bran oil, has been shown to be effective in increasing insulin sensitivity, reducing weight, and alleviating oxidative stress. In modern Chinese medicine, 香格里拉谷维素胶囊, 纯净谷维素软胶囊, and 诺特博谷维素片 are some of the medicines marketed in China that contain gamma-oryzanol. This study aims to evaluate the effects of gamma-oryzanol on clinical hallmarks of PCOS. Also to compare the efficacy of gamma-oryzanol alone versus in combination with clomifene citrate.</p></div><div><h3>Methods</h3><p>In this study, PCOS was induced in a rat model using estradiol valerate. Gamma-oryzanol was then administered both alone and in combination with clomifene citrate.</p></div><div><h3>Results</h3><p>The effects of gamma-oryzanol on estrous cyclicity, fertility, histopathological alterations, estradiol and testosterone levels, and oxidative parameters were evaluated. The results showed that gamma-oryzanol, both alone and in combination with clomifene citrate, had protective effects against the estradiol valerate-induced PCOS rat model. The combination of gamma-oryzanol and clomifene citrate was particularly effective, showing significantly better results than the standard treatment group (clomifene citrate alone).</p></div><div><h3>Discussion</h3><p>These results suggest that gamma-oryzanol could be used in combination with existing standard treatments to enhance the management of PCOS. However, further molecular and clinical studies are needed to confirm these findings.</p></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100506"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001489/pdfft?md5=5f6bcca05a354f4ecd74b244470761fd&pid=1-s2.0-S2667142524001489-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review on anticancer potential of traditional Chinese medicine (Yang Zheng XiaoJi)","authors":"Krishn Kumar Agrawal ,&nbsp;Yogesh Murti","doi":"10.1016/j.prmcm.2024.100505","DOIUrl":"10.1016/j.prmcm.2024.100505","url":null,"abstract":"<div><h3>Background</h3><p>The term \"cancer\" refers to illnesses in which cells can proliferate uncontrollably, infiltrate, and spread to various body organs. Since it has been used for thousands of years, traditional Chinese medicine (TCM) is now commonly regarded as an alternative for treating cancer. It has been demonstrated that Yangzheng Xiaoji (YX), a traditional Chinese medicine formulation, has anticancer effects in patients with different tumor types.</p></div><div><h3>Method</h3><p>Searches were conducted in several electronic databases from the year 2003 to 2024, including Pubmed, Google Scholar, Scopus, and Science Direct, to find evidence about YX. The purpose of this review is to provide an overview of YX's anticancer research findings.</p></div><div><h3>Result</h3><p>A result of the limited understanding of the fundamental mechanisms of action of the herbs, modern medicine frequently conflicts with traditional medicine, such as Chinese herbal medicine. Our research demonstrates the broad spectrum of anticancer potential of the YX formulation. Additionally, the particular YX component was also searched for anticancer potential.</p></div><div><h3>Discussion</h3><p>We have chosen to emphasize only some aspects of each YX ingredient's function in cancer therapy because it has been suggested that several processes are involved when applying TCM therapies. Furthermore, the effect of YX therapy on various malignancies is also covered. Additionally, we have emphasized YX treatment research that alters the tumor microenvironment and eradicates cancer.</p></div><div><h3>Conclusion</h3><p>The data gathered in this review will act as a strong basis for creating YX for cancer therapy.</p></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100505"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001477/pdfft?md5=18c624b34e4564bc87ee66ee00391502&pid=1-s2.0-S2667142524001477-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hehuan Anshen decoction inhibits ferroptosis to ameliorate p-Chlorophenylalanine-induced insomnia by activating GPX4 pathway","authors":"Qing Deng ,&nbsp;Yanan Li ,&nbsp;Wenyun Kui ,&nbsp;Linting He ,&nbsp;Yuxia Wang ,&nbsp;Nana Li ,&nbsp;Jian Xu ,&nbsp;Kaiqiang Wang ,&nbsp;Chunchun Xue ,&nbsp;Zhongwei Sha","doi":"10.1016/j.prmcm.2024.100504","DOIUrl":"10.1016/j.prmcm.2024.100504","url":null,"abstract":"<div><h3>Introduction</h3><p>Insomnia is a chronic disease affecting a third of the global adult population. Hehuan Anshen Decoction (HHASD), an innovative formula derived from Traditional Chinese medicine, has demonstrated therapeutic efficacy in treating insomnia, however, the potential pharmacological mechanism underlying its anti-insomnia effects remains incompletely elucidated. This study aimed to explore the underlying mechanism of HHASD treatment in mice with insomnia induced by p-Chlorophenylalanine (PCPA).</p></div><div><h3>Methods</h3><p>The active constituents of HHASD were analysed by means of UPLC<img>HRMS. PCPA mice were administered HHASD orally for 7 days. The anti-insomnia phenotype of HHASD were assessed by behavioral tests, encompassing the open field test and pentobarbital sodium-induced sleep test, alongside the measurement of hypothalamic 5-HT levels. Then, we conducted an in-depth analysis of specific ferroptosis markers, considering biochemistry, genetics and morphology. Additionally, ferroptosis inhibitor RSL3 was used to observe the underlying mechanism of the effects of HHASD.</p></div><div><h3>Results</h3><p>HHASD could effectively improve the insomnia behaviors induced by PCPA, resulting in increased movement trajectories, decreased sleep latency and prolonged sleep duration. Specifically, HHASD exerted a neuroprotective effect by enhancing the integrity of Nissl bodies in the hypothalamus of the PCPA mice. Mechanistic analysis revealed that HHASD could reverse the hypothalamic ferroptosis phenotype of insomnia mice by restoring the lowered levels of glutathione (GSH), inhibiting iron accumulation and elevated malondialdehyde (MDA), and mitigating mitochondrial cristae damage. Furthermore, HHASD enhanced the expression of SLC7A11 and GPX4 and reduced the ASCL4 in the hypothalamus, while the anti-insomnia effect of HHASD in the PCPA mice was eliminated by the GPX4 inhibitor RLS3.</p></div><div><h3>Conclusion</h3><p>HHASD ameliorates insomnia-related behaviors and protects against neuronal damage by suppressing ferroptosis by regulation GPX4 signaling. This study is not only a valuable attempt to explore the potential mechanism of Traditional Chinese formula but also will provide a novel targeted therapy for the treatment strategy of insomnia.</p></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100504"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667142524001465/pdfft?md5=fc851356a17336fcd2a9eaf3831f7f37&pid=1-s2.0-S2667142524001465-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}