Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"Traditional chinese medicine as a source of anti-diabetic agents: Investigating the potential of Leptadenia reticulata and Marsdenia tenacissima roots","authors":"Sahaya Mercy Jaquline R ,&nbsp;Neeraj Kumar ,&nbsp;Nilajan Saha ,&nbsp;Vidhu Aeri","doi":"10.1016/j.prmcm.2025.100583","DOIUrl":"10.1016/j.prmcm.2025.100583","url":null,"abstract":"<div><h3>Background</h3><div>Diabetes mellitus, a globally prevalent metabolic disorder, often requires treatments focused on glycemic control but with limited long-term efficacy and side effects. Traditional Chinese Medicine (TCM) provides a holistic alternative, addressing metabolic imbalances and preventing complications. This study explores the antidiabetic potential of <em>Leptadenia reticulata</em> (Jivanti) and <em>Marsdenia tenacissima</em> (Tong-guang-san) in a streptozotocin-induced diabetic rat model.</div></div><div><h3>Methods and Materials</h3><div>The root's ethanolic and aqueous ethanolic extracts (8:2) were prepared using a static maceration process. The α-amylase, α-glucosidase inhibition, and anti-glycation assay were performed using the extracts (50 to 300 µg/mL). Further, the extracts (100 and 200 mg/kg b.w.) were subjected to <em>in-vivo</em> anti-diabetic activity in Wistar rats with streptozotocin-induced diabetes. The oral glucose tolerance test (OGTT) was performed, and the fasting blood glucose, biochemical parameters, and lipid profile were measured. After the experimental conditions, the rats were sacrificed for pathological changes in the kidney, pancreas, and liver.</div></div><div><h3>Main Findings</h3><div>The ethanolic extract of L. <em>reticulata</em> (IC₅₀ -106.414 µg/mL) showed good inhibition of α-amylase compared to the standard acarbose (IC₅₀ -120.74 µg/mL). While ethanolic extract of M<em>. tenacissima</em> showed excellent inhibition of α-glucosidase (IC₅₀ -89.645 µg/mL), and anti-glycation activity (IC₅₀ -99.66 µg/mL) at 300 µg/mL exhibiting postprandial hypoglycemic effect. The OGTT test revealed that the ethanolic extracts of the roots of both plants at 100 and 200 mg/kg b.w dose reduced the elevated blood glucose level and restored the elevated liver parameters significantly (<em>p</em> &lt; 0.001). The group treated with ethanolic extract of L. <em>reticulata</em> and M<em>. tenacissima</em> at 100 and 200 mg/kg b.w showed amelioration of the elevated parameters of total cholesterol, triglycerides, high-density lipoproteins, and low-density lipoproteins to baseline state significantly (<em>p</em> &lt; 0.001). On the other hand, the histopathological studies showed restoration of a bunch of β-cells, which stimulated the insulin and glucose uptake by tissue, thus reducing the glucose load and increasing clearance.</div></div><div><h3>Principal Conclusion</h3><div>These investigations revealed the anti-diabetic potential of the L. <em>reticulata</em> and M<em>. tenacissima</em> root extracts, which warrants further clinical and molecular studies to make them applicable to the management of diabetes.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100583"},"PeriodicalIF":0.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qingyihuaji formula prolongs overall survival time in patients with pancreatic cancer: A retrospective analysis","authors":"Libin Song ,&nbsp;Yu Zeng ,&nbsp;Chuanfang Pan ,&nbsp;Jing Xie ,&nbsp;Xiumei Zhang ,&nbsp;Jiling Feng ,&nbsp;Shengli Li","doi":"10.1016/j.prmcm.2025.100588","DOIUrl":"10.1016/j.prmcm.2025.100588","url":null,"abstract":"<div><h3>Introduction</h3><div>Pancreatic cancer (PC) is recognized for its aggressive nature and poor prognosis. Despite advancements in therapeutic approaches, the survival time for PC patients remains short. Consequently, there is a pressing need for innovative strategies to enhance outcomes and prolong the survival of individuals afflicted with PC.</div></div><div><h3>Methodology</h3><div>This study explores the impact of Qingyihuaji formula (QYHJ), a Chinese herbal medicine, on the survival duration of PC patients. We conduct a retrospective analysis of a 15-year cohort (2002–2017), comprising 2,986 PC patients who received QYHJ treatment. Patients were categorized based on the duration of QYHJ therapy: those treated for more than three months (<em>n</em> = 1,452; 48.6 %) and those treated for less than three months (<em>n</em> = 1,534; 51.4 %). Statistical methods were employed to evaluate survival outcomes, and subgroup analyses were conducted to assess the influence of clinical parameters on QYHJ's efficacy.</div></div><div><h3>Results</h3><div>Patients who underwent QYHJ treatment for over three months exhibited a significantly extended median survival time, surpassing 12 months. Additionally, the three-year survival rate increased from 1.63 % to 8.82 % in this group. Comparatively, QYHJ's protective effect was on par with surgical resection and exceeded the benefits with radiotherapy and chemotherapy. Subgroup analyses revealed that the efficacy of QYHJ was not affected by various clinical parameters, including age, gender, CA199 level, primary tumor site, metastasis status, and concurrent treatments such as radiotherapy, chemotherapy, and surgical intervention.</div></div><div><h3>Conclusion</h3><div>The retrospective analysis underscores that QYHJ treatment for a duration exceeding three months significantly enhances the survival time of PC patients, independent of major clinical characteristics. These findings suggest that QYHJ could serve as a promising adjunctive treatment for PC, with the potential to be integrated into existing therapeutic protocols. Further prospective studies are warranted to validate these results and facilitate the incorporation of QYHJ into standard PC treatment regimens.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100588"},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global research trends in Chinese medicine for the treatment of non-alcoholic fatty liver disease (NAFLD): A bibliometric study","authors":"Kangjie li ,&nbsp;Jie Wang ,&nbsp;Ming Dang ,&nbsp;Runxin Ren ,&nbsp;Maomao Fu ,&nbsp;Jing Wang ,&nbsp;Qiao Zhang ,&nbsp;Chongbo Zhao","doi":"10.1016/j.prmcm.2025.100585","DOIUrl":"10.1016/j.prmcm.2025.100585","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;The global incidence of non-alcoholic fatty liver disease (NAFLD) is rising due to changes in modern lifestyle and dietary habits, making it one of the leading causes of chronic liver disease. NAFLD can progress to more severe liver conditions, imposing significant economic burdens. Currently, no FDA-approved drugs are available for its treatment. However, Traditional Chinese Medicine (TCM) has gained attention for its unique theoretical framework and therapeutic strategies, particularly its potential to improve liver health by modulating liver function. Recent studies have also highlighted TCM's ability to regulate gut microbiota, offering a promising avenue for NAFLD management. To better understand the evolving role of TCM in treating NAFLD, a comprehensive analysis of existing research is necessary.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;In this study, the Web of Science database was chosen as the primary data source to investigate the treatment of non-alcoholic fatty liver disease (NAFLD) using traditional Chinese medicine (TCM). Specifically, the search focused on English articles and review articles indexed in the SCI-E and SSCI databases, spanning from January 1, 2012, to October 30, 2024. The search strategy employed the following formula: TS = (“non-alcoholic fatty liver disease” OR “Metabolic dysfunction-associated fatty liver disease” OR “NAFLD”) AND TS = (“TCM” OR “Herbal medicines”). The analysis encompassed various aspects such as authors, keywords, journals, countries, institutions, and references. For this purpose, analytical tools including VOSviewer, Bibliometrix, and CiteSpace were utilized to systematically evaluate the gathered data.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;A total of 306 documents were retrieved, indicating a rising trend in the number of publications, which is expected to accelerate in the future. China is at the forefront in terms of both the quantity of publications and total citations, demonstrating strong international collaboration. Within China, Shanghai University of TCM and Chengdu University of TCM are leading institutions, having established a stable group of collaborating authors. The top 10 key journals account for 49.02 % of the publications, with Frontiers in Pharmacology being the most prolific, while the World Journal of Gastroenterology boasts the highest average number of citations. The most frequently cited journals are Hepatology, Journal of Hepatology, and Journal of Ethnopharmacology. Keyword analysis reveals that high-frequency terms such as steatohepatitis are prevalent, addressing topics like pathogenesis, indirect therapeutic effects, and molecular biological mechanisms. Recent outbreak word analysis highlights 'cell' and 'steatohepatitis' as current hotspots, with 'NASH' anticipated to be a focal point in 2024.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Discussion&lt;/h3&gt;&lt;div&gt;The number of publications on TCM treatments for NAFLD has steadily increased, with TCM exhibiting therapeut","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100585"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143224975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"State of the art progress of Evolvulus alsinoides in pharmacological activity and plant tissue culture: A potent Chinese medicinal plant","authors":"Collince Omondi Awere ,&nbsp;Valentine Chikaodili Anadebe ,&nbsp;Rakkammal Kasinathan ,&nbsp;Pandiyan Muthuramalingam ,&nbsp;Ramesh Manikandan","doi":"10.1016/j.prmcm.2025.100586","DOIUrl":"10.1016/j.prmcm.2025.100586","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Evolvulus alsinoides</em> (Convolvulaceae family), commonly known as ‘tu ding gui’ in Chinese. It is a medicinal plant with strong roots in traditional Chinese medicine (TCM). Its rich secondary metabolite compositions, as well as therapeutic and medical properties, yet it has limited research. This has prompted scientific investigation into its potential pharmacological benefits and bridging the gap between conventional medicine and contemporary therapeutic advancements in pharmacological research.</div></div><div><h3>Methods</h3><div>A total of 60 papers were identified through internet databases such as Scopus, Google Scholar, Web of Science, and PubMed. Various keywords such as <em>E. alsinoides</em> micropropagation, phytochemistry, and pharmacology were utilized to search for information. This review extensively analyzes the applications of <em>E. alsinoides</em>, including extraction and quantification methodologies, secondary metabolites, and pharmacological activities.</div></div><div><h3>Results</h3><div><em>E. alsinoides</em> contain an abundance of secondary metabolites, such as triterpenoids, flavonoids, terpenoids, alkaloids, and essential oils, which contribute to its anti-inflammatory, antimicrobial, antioxidant, antiproliferative, and immunomodulatory properties. The existence of these bioactive chemicals is consistent with their historic usage in treating a variety of illnesses and neurological conditions.</div></div><div><h3>Discussion</h3><div><em>E. alsinoides</em> has enormous therapeutic potential beyond its traditional usage in Chinese medicine. The diverse medicinal profile shows that it has the potential to treat a variety of health issues. Nevertheless, thorough clinical trials and translational research are required to bridge the gap between conventional knowledge and current medical uses. This study provides a touchstone to integrate current information and identify areas for future research on <em>E. alsinoides</em> as a multifunctional plant, with applications in pharmacology and beyond.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100586"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the neuroprotective potential of Eucalyptus globulus essential oil in a 6-hydroxydopamine rat model of Parkinson's disease","authors":"Aracely Janneth Naranjo-Viteri ,&nbsp;Matías Jávega Cometto ,&nbsp;Matías Caverzan ,&nbsp;Leandro Gabriel Champarini ,&nbsp;María Paula Zunino ,&nbsp;Rogelio E. Abburra ,&nbsp;Claudia B. Hereñú ,&nbsp;Rosana Crespo","doi":"10.1016/j.prmcm.2025.100584","DOIUrl":"10.1016/j.prmcm.2025.100584","url":null,"abstract":"<div><h3>Introduction</h3><div>Essential oil (EO) of eucalyptus has been commonly used as a traditional Chinese medicine to treat illnesses such as influenza, dysentery and eczema, and it is widely used in the pharmaceutical industry. However, its neuroprotective effects have not been fully demonstrated. Given the rising prevalence of the neurodegenerative disorder, Parkinson's disease, in aging populations, this study aims to investigate the effect of Eucalyptus globulus EO (EuEO) in a rat Parkinson model induced by bilateral lesion with the neurotoxin, 6-hydroxydopamine (6-OHDA).</div></div><div><h3>Methods</h3><div>EuEO composition was determined by GC–MS. Wistar rats were treated with EuEO (25 mg/kg, i.p) for 36 days, and behavioral tests (Barnes maze, wire mesh ramp, and grip strength) were performed 18 days after 6-OHDA application. We evaluated tyrosine hydroxylase (TH) and dopamine transporter (DAT) expression in the brain caudate-putamen unit; inflammatory cytokine (TNF-α, IL-6) expression in the hippocampus; liver, kidney, and gut histology; serum parameters; and hepatic lipid content.</div></div><div><h3>Results</h3><div>Our findings indicate that EuEO is mainly composed of 1,8-cineole (81.8 %) and that, in the parkinsonian rats, it improved impairments in spatial memory, motor performance, and strength; increased TH and DAT protein content; decreased TNF-α expression; diminished liver lipid content and body weight gain without changes in the rats’ food/water consumption or their serum lipid levels; diminished transaminase and alkaline phosphatase activity, and the albumin/globulin ratio.</div></div><div><h3>Discussion</h3><div>Although 1,8-cineole is a well-documented bioactive compound, further research is required to evaluate its potential synergistic or additive interactions with minor components. Our findings highlight the remarkable neuroprotective potential of EuEO and encourage further research of this very promising therapeutic alternative.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100584"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143224971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The knowledge mapping of traditional Chinese medicine for hepatocellular carcinoma: A bibliometric study","authors":"Wenya Pei ,&nbsp;Boyu Jiao ,&nbsp;Zexiong Chen ,&nbsp;Jingchun Zeng ,&nbsp;Yancheng Fu ,&nbsp;Zhanmou Liang ,&nbsp;Baoguo Sun ,&nbsp;Jingwen Ruan ,&nbsp;Shijun Zhang","doi":"10.1016/j.prmcm.2025.100581","DOIUrl":"10.1016/j.prmcm.2025.100581","url":null,"abstract":"<div><h3>Introduction</h3><div>Traditional Chinese medicine is widely used as adjuvant therapy for hepatocellular carcinoma (HCC), but no relevant bibliometric study has comprehensively analyzed the available data.</div></div><div><h3>Methods</h3><div>To summarize the research landscape of traditional Chinese medicine (TCM) in relation to HCC over the past decade, publications on TCM and HCC from 2013 to 2023 were collected and analyzed using CiteSpace software.</div></div><div><h3>Results</h3><div>A total of 1,160 articles on TCM for HCC were included in the bibliometric analysis, with the most productive countries and institutions located in the Asia region. The analysis indicated that traditional Chinese medicine may enhance immunity and regulate the local tumor microenvironment through mechanisms involving DNA damage, cell cycle arrest, and apoptosis by signaling pathways.</div></div><div><h3>Discussion</h3><div>This study provides insights into the trends of traditional Chinese medicine for hepatocellular carcinoma, which may inform clinical practice and identify potential targets for further research. However, high-quality evidence-based research and rigorous mechanistic studies should be prioritized to explore new anticancer approaches.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100581"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143224974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic role of isoflavones from traditional medicine in the management of polycystic ovary syndrome","authors":"Ashwini Armarkar ,&nbsp;Prafulla Sabale ,&nbsp;Satyendra Prasad ,&nbsp;Vidya Sabale ,&nbsp;Lata Potey ,&nbsp;Dipti Masram","doi":"10.1016/j.prmcm.2025.100580","DOIUrl":"10.1016/j.prmcm.2025.100580","url":null,"abstract":"<div><h3>Introduction</h3><div>Polycystic ovary syndrome (PCOS) is a significant public health problem and one of the most common gynaecological and endocrine disorder.This condition affects an estimated 8–13 % of women of reproductive age worldwide. PCOS manifests as a group of symptoms, including oligomenorrhea, obesity, infertility, increased risk of diabetes, acne, and hirsutism. The etiology of PCOS involves a complex interplay of factors, including genetic predisposition, neuroendocrine dysregulation, contemporary lifestyle practices, and obesity. Numerous traditional chinese formulations have demonstrated success in mitigating PCOS symptoms and addressing its underlying causes.</div></div><div><h3>Methodology</h3><div>Online databases such as Google Scholar, PubMed, and ScienceDirect Library were employed to find literatures on “Polycystic ovary syndrome,” “Phytoestrogens,” “Pharmacokinetics of Isoflavones” Role of isoflavones in PCOS. considering articles published in the last 20 years to capture recent research.This review summarizes the scientific findings on traditional herbs rich in isoflavones and their bioactive components of the phytoestrogen class for the management of PCOS.</div></div><div><h3>Result</h3><div>Isoflavones, a subset of polyphenolic compounds, represent a prominent class of phytoestrogens. These secondary plant metabolites, which predominantly occur in the Fabaceae family, exhibit structural similarities to 17β-estradiol, and typically exist in conjugated forms. The estrogenic properties of isoflavone phytoconstituents, such as genistein, biochanin A, daidzein, puerarin, and formononetin, confer substantial therapeutic potential for PCOS management.</div></div><div><h3>Conclusion</h3><div>Despite their therapeutic potential, there is a paucity of data regarding the safety profile, clinical efficacy, and optimal treatment duration of isoflavones. Further investigations across various domains of isoflavone research are imperative to enhance the understanding of this complex field of study.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100580"},"PeriodicalIF":0.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143224545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of extraction parameters and anti-hyperglycemic assessment of standardized extract from Santalum album L. leaves","authors":"Phan Thi Kim Phung ,&nbsp;Dang Ngoc Lam Binh ,&nbsp;Nguyen Thi Anh Thu ,&nbsp;Nguyen Van Ha ,&nbsp;Tran Thi Huyen ,&nbsp;Khuu Minh Hien ,&nbsp;Mai Huynh Nhu ,&nbsp;Ngo Kien Duc ,&nbsp;Le Minh Quan","doi":"10.1016/j.prmcm.2025.100577","DOIUrl":"10.1016/j.prmcm.2025.100577","url":null,"abstract":"<div><h3>Background</h3><div><em>Santalum album</em> (SA) L., also known as sandalwood, a habituated plant in Asia and China, is commonly used in traditional Chinese medicine to treat various diseases, including inflammation and diarrhea. It is a versatile tree with considerable commercial worth, primarily due to its exceptionally fragrant qualities. Although extensively studied worldwide for its pharmacological effects, research in Vietnam has yet to develop optimized extraction processes or investigate the biological activities of SA L. constituents.</div></div><div><h3>Aim of the study</h3><div>The objectives of this study were to establish standards, optimize extraction processes, and evaluate the hypoglycemic effects of extracts from the leaves of SA.</div></div><div><h3>Materials and methods</h3><div>The extraction parameters for vitexin and isovitexin were optimized using response surface methodology. Vitexin and isovitexin were quantified by ultra-high-performance liquid chromatography. The hypoglycemic effect of SA extract was assessed using a male Swiss albino mouse model.</div></div><div><h3>Results and discussion</h3><div>Quantification of vitexin and isovitexin in SA extract met the validation standards. The optimal extraction process involved refluxing at 66 °C for 180 min, using 70 % ethanol at a solvent-to-material ratio of 33:1 mL/g. Standardized extracts were developed using specified criteria for sensory properties, moisture, heavy metals, microbial limits, and compound identification. The SA extract contained 6.61 % vitexin and 4.47 % isovitexin (w/w). Safe oral SA doses of 1 and 2 g/kg were established, which improved body weight and blood glucose levels in rats with hyperglycemia.</div></div><div><h3>Conclusion</h3><div>These findings suggest that the optimized method is effective for the extraction of vitexin and isovitexin from SA L. leaves and that the plant may serve as a potential candidate for the development of a new natural anti-diabetic agent.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100577"},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating network pharmacology and experimental validation to explore the mechanism of action of BushenQiangxin Formula for the treatment of chronic heart failure","authors":"Jinhua Pang ,&nbsp;Jiejun Hou ,&nbsp;Jinyi Chen ,&nbsp;Wenchu Zhao ,&nbsp;Tiantian Tang ,&nbsp;Taotao Li ,&nbsp;Ding Liu ,&nbsp;Jinkai Li ,&nbsp;Xuan Wang","doi":"10.1016/j.prmcm.2025.100579","DOIUrl":"10.1016/j.prmcm.2025.100579","url":null,"abstract":"<div><h3>Objective</h3><div>: To evaluate the mechanism of action of <strong>the BushenQiangxin (BSQX) formula</strong> in treating chronic heart failure (CHF) based on network pharmacology and experimental zoology.</div></div><div><h3>Methods</h3><div>: The targets and components of BSQX in CHF were assessed based on network pharmacology and data from the Gene Expression Omnibus (GEO) database. The drug–disease interaction network diagram was constructed, and the possible pathways affected by BSQX in CHF were analyzed. Molecular docking between the key components of BSQX and the key targets of CHF was performed using the Discovery Studio software. Finally, a CHF rat model was established. The serum interleukin (IL)-6 level was evaluated using the enzyme-linked immunosorbent assay in each rat group. The structural changes in the left ventricular myocardial tissues were observed via hematoxylin &amp; eosin staining, and the expression of cTnI, IL-17, and nuclear factor kappa B (NF-KB) proteins was measured via immunohistochemistry in the cardiac muscle tissue.</div></div><div><h3>Results</h3><div>: Network pharmacology and data from the GSE84796 database were used to screen 25 core targets of BSQX for CHF. The major signaling pathways, including the IL-17 signaling pathway, tumor necrosis factor signaling pathway, AGE-RAGE signaling pathway, which are associated with diabetic complications, lipid, and atherosclerosis were identified via Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. The molecular docking results showed good binding between the core targets (IL6, MMP9, CCL2, and STAT1) and active compounds (such as quercetin, lignocerol, oleanolic acid, pentacosanoic acid, and isobetulinic acid). Hematoxylin &amp; eosin staining in animal experiments showed that BSQX could reduce the degree of inflammatory infiltration and fibrosis of cardiomyocytes in CHF to a certain extent. The enzyme-linked immunosorbent assay and immunohistochemistry showed that BSQX could effectively reduce the levels of IL-17, IL-6, cTnI, and NF-KB in chronic rats.</div></div><div><h3>Conclusion</h3><div>: In CHF treatment, the mechanism of BSQX can be related to the inhibition of IL-17, IL-6, cTnI, and NF-KB production, inflammatory cell infiltration, and the attenuation of myocardial injury based on network pharmacology and experimental animal studies. Moreover, its mechanism of action may function via the IL-17 signaling pathway.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100579"},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143138030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginseng exhibits adaptogenic-like activity in mice exposed to hypoxic-anoxic stress through activation of antioxidant/BDNF protective mechanisms and inhibition of pro-inflammatory cytokines/NF-KB signaling pathways","authors":"James Olukayode Oni ,&nbsp;Divine Oluwaferanmi Oyenekan ,&nbsp;Yetunde Olamide Olayemi ,&nbsp;Osarugue Christabel Irabor ,&nbsp;Precious Ogbuji ,&nbsp;McCarthy Oritseweyinmi Tova ,&nbsp;Paul Ademola Adeleke ,&nbsp;Gbemisayo Adetomiwa Abbas ,&nbsp;Solomon Umukoro","doi":"10.1016/j.prmcm.2025.100578","DOIUrl":"10.1016/j.prmcm.2025.100578","url":null,"abstract":"<div><h3>Introduction</h3><div>Hypoxic-anoxic stress causes severe injury to the brain cells resulting in various neuropathological complications. Ginseng is widely used in Traditional Chinese Medicine (TCM) as a tonic for healthy living due to its adaptogenic/rejuvenating properties and in mitigating chronic stress-related diseases including vertigo, depression, short-term memory loss, and lack of attention or vigilance. This study evaluates the adaptogenic-like properties of <em>Panax ginseng</em> commercial product (Ginsomin) and the biochemical mechanisms underlying its action in male Swiss mice exposed to hypoxic-anoxic stress.</div></div><div><h3>Methods</h3><div>. The mice (20–22 g) were distributed into five groups (<em>n</em> = 6). Groups 1 (non-stress control) and 2 (stress-control) received distilled water while groups 3–5 had ginseng (10, 50, and 100 mg/kg), orally for seven days. Groups 2 to 5 were subjected to hypoxic stress for 20 min for six days prior to assessment of the latency to anoxic convulsion on day 7. The neurobehavioral changes (motor functions, memory, anxiety, catalepsy, depression, and muscle strength) were evaluated afterwards. Blood glucose and corticosterone levels alongside brain contents of malondialdehyde, nitrites, antioxidant profiles, pro-inflammatory cytokines (tumor necrosis factor–alpha and interleukin-6), glutamate, brain derived neurotrophic factor (BDNF), and nuclear factor kappa B (NF-κB) were determined. The brain acetylcholinesterase, caspase-3, myeloperoxidase, nuclear factor erythroid 2-related factor 2 (NFr-2) and glutamic acid decarboxylase (GAD) activities were evaluated.</div></div><div><h3>Results</h3><div>. Ginseng attenuated the anoxic convulsion and neurobehavioral deficits in stressed-mice. It reduced plasma corticosterone and glucose levels, and decreased the brain contents of malondialdehyde, nitrites, pro-inflammatory cytokines, NF-kB, caspase-3, acetylcholinesterase, glutamate and myeloperoxidase. Ginseng-treated mice had increased brain antioxidant profiles; GAD, BDNF, and NFr-2 contents relative to stress-control.</div></div><div><h3>Discussion</h3><div>. The results of the study showed that ginseng demonstrated adaptogenic-like properties by attenuating neurobehavioral deficits in mice exposed to hypoxic-anoxic stress via multiple molecular mechanisms, relating to inhibition of corticosterone-mediated oxidative and inflammatory/apoptotic signaling pathways, and activation of GAD, BDNF and NFr-2 stress-protective defense systems. Taken together, these findings further confirm its adaptogenic-like properties in alleviating common geriatric chronic stress-related diseases in Chinese medicines.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100578"},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}