Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"Exploring the mechanism of Taohong Siwu decoction in the treatment of hyperlipidemia based on network pharmacology and zebrafish models","authors":"Mingyang Li ,&nbsp;Xinmei Zhang ,&nbsp;Chunyue Bao ,&nbsp;Fan Li ,&nbsp;Yulong Wu ,&nbsp;Guangming Huo ,&nbsp;Chuanfeng Tang ,&nbsp;Jianmei Li","doi":"10.1016/j.prmcm.2025.100600","DOIUrl":"10.1016/j.prmcm.2025.100600","url":null,"abstract":"<div><h3>Introduction</h3><div>Taohong Siwu Decoction (TSD) is a traditional Chinese medicine used to treat cardiovascular diseases and blood stasis. This study explores the therapeutic mechanisms of TSD in treating hyperlipidemia using a network pharmacology approach combined with a zebrafish hyperlipidemia model.</div></div><div><h3>Methods</h3><div>Compounds in TSD were sourced from a database and filtered based on oral bioavailability and drug-likeness. Herbal targets were predicted using online tools, and hyperlipidemia-related genes were identified via GeneCards. Pathway analysis was conducted to pinpoint relevant signaling pathways. Molecular docking, performed with AutoDock, assessed the binding affinity of key compounds to target proteins. In vivo experiments using zebrafish models evaluated the anti-hyperlipidemic, anti-inflammatory, and antioxidant effects of TSD, with RT-qPCR used to verify the expression of predicted targets.</div></div><div><h3>Results</h3><div>Network pharmacology analysis revealed 45 bioactive phytochemicals and 72 potential target genes implicated in hyperlipidemia-related pathways. Six principal bioactive compounds—quercetin, luteolin, myricanone, stigmasterol, kaempferol, and β-sitosterol—were identified as modulators of core therapeutic targets including TNF, IL6, IL1B, PTGS2, PPARG, ESR1, PTGS1, and PIK3CG, influencing critical pathways associated with inflammatory responses, oxidative stress modulation, and lipid metabolism regulation. Molecular docking analysis demonstrated robust binding affinities between these compounds and their respective targets, particularly PTGS2 and PIK3CG. Zebrafish experiments substantiated TSD's therapeutic efficacy in ameliorating hyperlipidemia, attenuating inflammation, and mitigating oxidative stress, thereby validating the predicted mechanisms of action.</div></div><div><h3>Discussion/Conclusions</h3><div>TSD exhibits a characteristic multi-component, multi-target, and multi-pathway therapeutic profile in the management of hyperlipidemia and associated atherosclerotic conditions. These findings support its clinical application and provide a theoretical basis for future research.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100600"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Cananga odorata modulates cholinergic, purinergic enzymes, and elevates antioxidant markers in diabetes-induced cognitive disorder rat brain","authors":"Jamiyu A. Saliu ,&nbsp;Olajide R. Ojo ,&nbsp;Idowu S. Oyeleye ,&nbsp;Ganiyu Oboh","doi":"10.1016/j.prmcm.2025.100607","DOIUrl":"10.1016/j.prmcm.2025.100607","url":null,"abstract":"<div><h3>Introduction</h3><div>In the folklore of Chinese traditional medicine, therapeutic materials like <em>Cananga odorata</em> have several attributes that ameliorate non-communicable diseases. This study sought to examine the anti-cognitive dysfunction potential of <em>Cananga odorata</em> leaf extract (COLE) in the brain of diabetes-induced albino male rats.</div></div><div><h3>Methodology</h3><div>Forty-two mature male Wistar rats weighing between 200 to 250 g (<em>n</em> = 6) were used. They were split into seven groups: Normal control; DM (STZ 50 mg/kg via I.P) group; DM + Acarbose (25 mg/kg BW); DM + 2 mg, DM + 4 mg COLE, 2 mg COLE, and 4 mg COLE<em>.</em> Fasting blood glucose level was determined after 72 h. Behavioral training was conducted on the 14th day of the treatment and the rats were sacrificed. Biochemical indices acetylcholinesterase (AChE), butyrylcholinesterase (BChE), adenosine deaminase (ADA), reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), glutathione (GSH), and superoxide dismutase (SOD) were carried out on the brain tissue samples collected.</div></div><div><h3>Results</h3><div>The trial results show a significant decrease in the fasting blood glucose levels (FBGL) in the treatment groups. DM rats treated with COLE demonstrated better cognitive performance in the Y-maze behavior test, with considerable increases in alternation behavior. The DM group exhibited considerably higher levels of neurotransmitter enzyme activities, particularly AChE and BChE. However, the administration of COLE therapy attenuated these activities, indicating a protective impact on cholinergic function. Furthermore, the purinergic signaling-related enzymes ATPase and ADA showed decreased activity upon treatment with COLE. ROS and TBARS were considerably higher in the DM group but were successfully decreased by COLE treatment. The DM group had reduced levels of GSH and SOD activity, which was recovered with COLE. This suggests an improvement in the brain's antioxidant defense mechanism. The typical anti-diabetic medication used as a comparator, ACA, likewise showed efficacy but fell short of the effects of a higher dosage of COLE.</div></div><div><h3>Conclusion</h3><div>In DM-induced cognitive dysfunction, COLE, especially at a dosage of 4 mg, dramatically reduces hyperglycemia, modifies neurotransmitter enzyme activity, and strengthens antioxidant defenses. These results imply that COLE may be useful as a therapeutic agent to treat diabetes-related cognitive deficits.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100607"},"PeriodicalIF":0.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicine in treating pox: Insights for basic and clinical research of Mpox","authors":"Mei Lu ,&nbsp;Yi Ying ,&nbsp;Luming Xia ,&nbsp;Lu Gao ,&nbsp;Quangang Xu ,&nbsp;Yi Zhang","doi":"10.1016/j.prmcm.2025.100602","DOIUrl":"10.1016/j.prmcm.2025.100602","url":null,"abstract":"<div><h3>Introduction</h3><div>Mpox is rapidly spreading, posing a significant threat to public health. However, we were not prepared to deal with this re-emerging infectious disease. Traditional Chinese medicine (TCM) has long been used to treat emerging infectious diseases in China. It is worth investigating and debating whether TCM is a viable therapy option for mpox. This review aims to primarily describe the clinical evidence of TCM in the treatment of the pox virus, as well as the related antiviral mechanisms, to explore the potential of TCM in the treatment of mpox.</div></div><div><h3>Methods</h3><div>A comprehensive literature search was conducted to identify relevant studies on the use of TCM in treating poxvirus infections. Clinical trials, case reports, and mechanistic studies were included. Data on the effectiveness of TCM treatments, as well as the molecular and cellular mechanisms of action, were extracted and analyzed.</div></div><div><h3>Results</h3><div>Clinical evidence shows that TCM has considerable clinical effectiveness against poxvirus. TCM-derived medicinal compounds such as mimosine, quercetin, and miRNAs could inhibit viral replication by targeting viral genes or enzymes. TCMs have the benefit of being multitarget, multipathway, and multicomponent in the treatment of poxvirus. Furthermore, a number of TCM-prospective medications for the treatment of mpox were disclosed.</div></div><div><h3>Discussion</h3><div>The results suggest that TCM has enormous potential in the treatment of mpox. The multitarget and multipathway nature of TCM offers a unique advantage in combating complex viral infections. However, there are existing problems such as the lack of standardized TCM preparations and the need for more rigorous clinical trials. Initiatives for improved drug development should focus on standardization and validation of TCM treatments. Overall, this review provides theoretical guidance for future TCM research on mpox therapy, and it is likely to inspire research on a potential avenue of drug discovery for mpox treatment. Further studies are needed to fully realize the potential of TCM in the treatment of mpox.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100602"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating network pharmacology, molecular docking, and experimental verification to demonstrate that Jiawei Duhuo Parasitic Decoction treated osteoarthritis by inhibiting PTGS2 expression","authors":"Yang Duan ,&nbsp;Li Jin ,&nbsp;Cheng Yu ,&nbsp;Weizhong Qi ,&nbsp;Songjia Ni","doi":"10.1016/j.prmcm.2025.100601","DOIUrl":"10.1016/j.prmcm.2025.100601","url":null,"abstract":"<div><h3>Introduction</h3><div>Jiawei Duhuo Parasitic Decoction (JDPD) is a traditional Chinese medicine commonly used to treat osteoarthritis (OA). However, the specific mechanisms by which JDPD acts against OA have not been fully elucidated. This study aimed to explore the potential mechanisms through which JDPD inhibits the onset and progression of OA.</div></div><div><h3>Methods</h3><div>The active components and targets of JDPD were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and Traditional Chinese Medicine Information Database (TCMID) databases. Potential OA-related targets were obtained from GeneCards, DisGeNET, DrugBank, and OMIM databases. The overlapping targets between JDPD and OA were analyzed using a protein - protein interaction (PPI) network and MCODE subnetwork, and central gene targets were identified through topological analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted using the Database for Annotation, Visualization and Integrated Discovery (DAVID) database. Subsequently, an active ingredient-target-pathway network was then constructed and validated through molecular docking. Finally, in vitro and in vivo models of OA-induced cartilage injury were established to verify the potential mechanisms by which JDPD inhibits OA-related cartilage damage.</div></div><div><h3>Results</h3><div>A total of 205 active components and 68 OA-related targets of JDPD were identified. Further analysis revealed eighteen key targets, primarily associated with therapeutic effects related to the expression of inflammatory factors and cell proliferation. The active ingredient-target-pathway network was constructed and validated using molecular docking. Finally, in vitro and in vivo experiments demonstrated that JDPD ameliorates OA-induced cartilage damage by inhibiting prostaglandin-endoperoxide synthase 2 (PTGS2)-mediated chondrocyte inflammation and extracellular matrix degradation.</div></div><div><h3>Discussion</h3><div>Our findings suggest that JDPD may treat OA through a multi-component, multi-target mechanism, with PTGS2 identified as a validated target. These results provide an experimental basis for the potential development of JDPD as a therapeutic agent for OA in the future.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100601"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of anti-inflammatory, antioxidant and antibacterial activities of epigallocatechin gallate, epigallocatechin, epicatechin gallate and epicatechin in vitro","authors":"Shuqi Wang ,&nbsp;Yu Chang ,&nbsp;Jingji Xu ,&nbsp;Chenru Zhao ,&nbsp;Yang Song ,&nbsp;Mingchun Liu ,&nbsp;Chunlian Tian","doi":"10.1016/j.prmcm.2025.100599","DOIUrl":"10.1016/j.prmcm.2025.100599","url":null,"abstract":"<div><h3>Introduction</h3><div>catechin compounds are flavanols substance from various natural plants, and play a significant part in anti-inflammatory, anti-tumor, anti-oxidation, anti-bacteria and protecting the heart and brain organs and other pharmacological effects, and widely found in traditional Chinese medicine, such as <em>Kadsurae Caulis</em> and <em>Reynoutria japonica</em> Houtt.</div></div><div><h3>Methods</h3><div>this research was focused on the comparative study of anti-inflammatory, antioxidant and antibacterial activities of (-)-epigallocatechin gallate, (-)-epigallocatechin, (-)-epicatechin gallate and (-)-epicatechin <em>in vitro</em>.</div></div><div><h3>Results</h3><div>the results indicated that the antioxidant activities of the four catechin compounds improved with the concentration increasing from 0.5 to 32 μg/ml, and the antibacterial activities of four catechins were stronger against the gram-positive bacteria (<em>Staphylococcus aureus</em> and <em>Arcanobacterium pyogenes</em>) than the gram-negative bacteria (<em>Escherichia coli</em> and <em>Salmonella</em>). Moreover, 12.5, 25 and 50 μmol/l of (-)-epicatechin, (-)-epigallocatechin gallate, and 6.25, 12.5 and 25 µmol/L of (-)-epigallocatechin, (-)-epicatechin gallate could reduce the content of NO and phagocytosis.</div></div><div><h3>Discussion</h3><div>these results laid a foundation for the research and development of natural anti-inflammatory, antioxidant and antibacterial drugs in food and pharmaceutical industries.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100599"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-VEGF combined with fufang xueshuangtong versus anti-VEGF monotherapy for wet age-related macular degeneration: A meta-analysis","authors":"Tingke Xie ,&nbsp;Yanyan Lan ,&nbsp;Chengming Chen ,&nbsp;Jing Han","doi":"10.1016/j.prmcm.2025.100598","DOIUrl":"10.1016/j.prmcm.2025.100598","url":null,"abstract":"<div><h3>Purpose</h3><div>To evaluate the effectiveness and safety of combining anti-VEGF and fufang xueshuangtong in the treatment of wet age-related macular degeneration (wet AMD).</div></div><div><h3>Methods</h3><div>A systematic review of studies focusing on the efficacy and safety of the combined anti-VEGF and Fufang Xueshuantong therapy versus anti-VEGF monotherapy for wet AMD was conducted. This meta-analysis was performed based on a protocol registered in PROSPERO (CRD42024593135). The outcomes were reported as weighted mean differences (MDs) with corresponding 95 % confidence intervals (CI) or risk ratio (RR) with 95 % CI.</div></div><div><h3>Results</h3><div>A total of 9 studies, involving 731 participants, were included. The MD for best-corrected visual acuity (BCVA) improvement, central macular thickness (CMT), and size of choroidal neovascularization (CNV) between the combination therapy and monotherapy groups were 7.37 (95 % CI [5.35 to 9.39]), −35.57 (95 % CI [−47.02 to −24.12]), and −1.81 (95 % CI [−2.56 to −1.07]), respectively. Additionally, the combination therapy group showed greater improvement in serum and ocular hemodynamics and had a lesser impact on intraocular pressure, and there was no significant difference in the incidence of adverse reactions between the two regimens.</div></div><div><h3>Conclusions</h3><div>The combination of anti-VEGF therapy with Fufang Xueshuantong provides superior efficacy compared to anti-VEGF monotherapy in the treatment of wet AMD and is associated with an improved safety profile. However, further trials are needed to enhance the robustness and reliability of these findings.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100598"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Exploring the efficacy and mechanistic action of traditional Chinese medicine-derived phytochemicals in rheumatoid arthritis”","authors":"Uma Palanikumar ,&nbsp;Rajagopal Balasubramanian ,&nbsp;Prasanna Seenivasan ,&nbsp;Vellaikumar Sampathrajan ,&nbsp;Thangavelu AU","doi":"10.1016/j.prmcm.2025.100597","DOIUrl":"10.1016/j.prmcm.2025.100597","url":null,"abstract":"<div><h3>Introduction</h3><div>Rheumatoid arthritis [RA] is an autoimmune chronic inflammatory condition that leads to joint degeneration and functional disability. The most advanced pharmacological therapies that have been available for a long time remain limited, as they are associated with adverse effects and incomplete disease remission. In contrast, Traditional Chinese Medicine [TCM] is a promising alternative, as it utilizes phytochemicals obtained from medicinal plants.</div></div><div><h3>Methods</h3><div>This study explores the efficacy and mechanistic action of TCM-derived phytochemicals in the management of RA, with insights on <em>in silico</em> approaches, including molecular docking, ADME [Absorption, Distribution, Metabolism, and Excretion] studies, and network-based analysis using Cytoscape. We evaluated the interactions of flavonoids, alkaloids, terpenoids, and glycoside phytochemicals from key TCM plants with critical targets in RA, like pro-inflammatory cytokines [TNF-α, IL-1β], enzymes [COX-2, MMP-9], and signaling pathways [NF-κB, JAK/STAT], considering the applications of computational tools.</div></div><div><h3>Results</h3><div>The available evidence indicates that TCM phytochemicals modulate inflammation, inhibit key enzymes, and enhance anti-inflammatory responses for multi-target therapy of RA. <em>In silico</em> evidence of the efficacy and mechanistic actions is supportive of the promising therapeutic potential of TCM phytochemicals.</div></div><div><h3>Discussion</h3><div>Despite their numerous therapeutic advantages, phytochemicals also present certain limitations, such as poor oral bioavailability, off-target interactions, and manufacturing challenges. These factors necessitate significant structural modifications before their potential use as drugs. In addition, this study underscores the importance of further experimental and clinical validation.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100597"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro & in-vivo efficacy of liposomal β-carryophyllene in ethylene glycol induced urolithiasis in rats","authors":"Ranjith Kumar R ,&nbsp;Suresh Janadri ,&nbsp;Manjunatha PM ,&nbsp;Madhu M V ,&nbsp;Rakshitha K B ,&nbsp;Preeti P Angadi ,&nbsp;Uday Raj Sharma ,&nbsp;Surrendra Vada ,&nbsp;Nageena Taj ,&nbsp;Jyotsna S Kharvi","doi":"10.1016/j.prmcm.2025.100587","DOIUrl":"10.1016/j.prmcm.2025.100587","url":null,"abstract":"<div><h3>Background</h3><div>Urolithiasis refers to the formation of stones in the urinary system. β-Caryophyllene, a bicyclic sesquiterpene found in herbs like cloves (Dīng Zǐ Xiāng), cinnamon (Ròu Guì), Black Pepper (Hú Jiāo) and Ginseng (Rén Shēn). Despite its potential, the antiurolithiatic activity of β-Caryophyllene, particularly in liposomal formulations, remains unexplored. This study aimed to evaluate the antiurolithiatic effects of liposomal β-Caryophyllene in ethylene glycol-induced urolithiasis in Wistar albino rats and elucidate its underlying mechanisms.</div></div><div><h3>Materials &amp; methods</h3><div><strong><em>IN-VITRO</em>:</strong> Using various in-vitro methods like aggregation, nucleation and titrimetric assay the percentage inhibition of calcium oxalate using the calcium chloride (Caox) and sodium oxalate solutions against the liposomal β-carryophyllene was performed for assessing anti-urolithiatic effect.</div><div><strong><em>IN-VIVO</em>:</strong> Ethylene glycol was used as the inducing agent. Albino rats were separated into 6 groups. Group-I named as normal diet. Group II-VI was given 0.75 % v/v EG was mixed in drinking water for causing renal stones, along with group III- V was treated with liposomal β-carryophyllene 100 mg/kg, 200 mg/kg and 400 mg/kg respectively. Group-VI was treated with standard drug, cystone (750 mg/kg).</div></div><div><h3>Results</h3><div>The study proved that liposomal β-caryophyllene (BCP) significantly inhibits calcium oxalate crystal formation, aggregation, and dissolution in both <em>in-vitro</em> and <em>in-vivo</em> models. BCP significantly reduced serum and urine markers of renal dysfunction and oxidative stress, with the highest dose (400 mg/kg) demonstrating effects similar to the standard drug (cystone).</div></div><div><h3>Conclusion</h3><div>Liposomal β-caryophyllene (BCP) shows promising therapeutic agent for urolithiasis, effectively preventing crystal formation and improving renal function. It demonstrated comparable efficacy to the standard treatment, suggesting its potential as a viable alternative or complementary therapy for managing urolithiasis.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100587"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143553002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tanyu Tongzhi decoction ameliorates atherosclerosis by inhibiting trimethylamine N-oxide-induced vascular inflammation via PI3K/Akt/NF-κB pathway regulation","authors":"Yingying Li ,&nbsp;Haonan Wu ,&nbsp;Ruoqi Li ,&nbsp;Wenjing Zong ,&nbsp;Xiang Li ,&nbsp;Huamin Zhang ,&nbsp;Danli Tang","doi":"10.1016/j.prmcm.2025.100582","DOIUrl":"10.1016/j.prmcm.2025.100582","url":null,"abstract":"<div><h3>Introduction</h3><div>Tanyu Tongzhi decoction (TYTZD) is an orally administered traditional Chinese medicine formula that is used to treat atherosclerosis (AS). Trimethylamine N-oxide (TMAO), a gut microbial metabolite, has been shown to accelerate AS development and progression. Despite its various health benefits, it is unclear whether TYTZD can ameliorate TMAO-mediated vascular inflammation. Therefore, we investigated the effect and molecular mechanisms of TYTZD in TMAO-induced vascular inflammation during AS progression.</div></div><div><h3>Methods</h3><div>Key ingredients of TYTZD were detected using high-performance liquid chromatography (HPLC). Additionally, we established an AS model by feeding <em>ApoE^-/-</em> mice high-fat and high-choline diet (HFCD). We examined the anti-AS effects of TYTZD using histological assay, immunohistochemical staining, and western blotting. Furthermore, we examined the effect of TYTZD on the viability of human umbilical vein endothelial cells (HUVECs) treated with TMAO using CCK-8 assay.</div></div><div><h3>Results</h3><div><em>In vivo</em> experiment showed that TYTZD treatment ameliorated AS by preventing dyslipidemia, reducing lipid deposition area in the intimal plaque, inhibiting arterial inflammation, and suppressing serum levels of TMA and TMAO in <em>ApoE^-/-</em> mice. Additionally, TYTZD significantly inhibited TMAO-induced endothelial activation, suppressed the expression of pro-inflammatory factors (IL-1β, IL-6, IL-8, TNF-α and MCP-1), and downregulated PI3K, Akt, and NF-κB protein expression in HUVECs.</div></div><div><h3>Conclusions</h3><div>TYTZD ameliorates AS by inhibiting TMAO-induced vascular inflammation via the PI3K/Akt/NF-κB signaling pathway; these findings highlight its potential application for the treatment of AS and associated disorders.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100582"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicine Yiqi Huoxue Huatan recipe inhibits atherosclerosis by regulating intestinal flora and its associated metabolites","authors":"Hongtao Huang ,&nbsp;Wenqing Lv ,&nbsp;Hanjun Zhao ,&nbsp;Feiyue Xu ,&nbsp;Yu Huang","doi":"10.1016/j.prmcm.2025.100593","DOIUrl":"10.1016/j.prmcm.2025.100593","url":null,"abstract":"<div><h3>Background</h3><div>Yiqi Huoxue Huatan recipe (YHHR) has been shown to possess anti-atherosclerotic effects, as detailed in various studies and clinical observations. However, the potential mechanism underlying atherosclerosis remains unclear. This study aimed to verify the effects of YHHR on gut microbiota composition and plasma metabolite levels in atherosclerotic mice and its mechanism of action.</div></div><div><h3>Materials and Methods</h3><div>SPF apoE-/- mice were fed high-fat chow for 12 weeks to build an atherosclerosis model, Additionally, blank control group mice were fed normal chow, Mice were randomly assigned to the model group, low, medium and high dose YHHR group, antibiotic group and antibiotic + medium dose YHHR group. After three courses of drug administration, aortic plaques were observed by hematoxylin and eosin (HE) and Oil Red O staining, and mouse feces and serum were collected. The feces and serum of mice were analyzed using 16S rRNA sequencing, microbiological analysis, and targeted metabolomics. For cellular experiments, by constructing a foam cell model and applyingglycoursodeoxycholic acid(GUDCA)and Compound C interventions, WB was used to detect macrophage AMPK signaling pathway-related protein (AMPK) and efferocytosis-associated protein (ProS).</div></div><div><h3>Results</h3><div>YHHR was able to attenuate atherosclerotic plaque area, up-regulate the levels of gut bacteria <em>Akkermansia muciniphila (A. muciniphila)</em> and gut bacteria-associated metabolite bile acids GUDCA, and promote macrophage efferocytosis through the AMPK signaling pathway to exert anti-AS effects.</div></div><div><h3>Conclusion</h3><div>YHHR may play a role in preventing atherosclerosis by influencing the production of the intestinal flora and its associated metabolites.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100593"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}