New CropsPub Date : 2024-07-01DOI: 10.1016/j.ncrops.2024.100032
Wenwen Duan , Kaiwen Li , Jialu Li , Ning Ding , Suting Wang , Yaling Zou , Zihao Zhang , Zhikun Duan , Jingjing Xing
{"title":"Exploring membrane proteins dynamic in plant cells with fluorescence correlation spectroscopy","authors":"Wenwen Duan , Kaiwen Li , Jialu Li , Ning Ding , Suting Wang , Yaling Zou , Zihao Zhang , Zhikun Duan , Jingjing Xing","doi":"10.1016/j.ncrops.2024.100032","DOIUrl":"10.1016/j.ncrops.2024.100032","url":null,"abstract":"<div><p>Biomolecule interactions and macromolecular rearrangement participate in numerous cellular functions in plants, and resolving the dynamics of plasma membrane proteins represents a central goal in current plant biology. Compared to yeast and mammalian systems, the quantification of heterogeneous distribution and dynamics of membrane proteins in cellular processes remains sparse in plant cells. In this study, we introduce the application of fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) in measuring membrane protein diffusion, concentration and interactions in living plant cell. The review showed FCS/FCCS as a tool for imaging the membrane proteins fused with a fluorescent tag, quantifying the density fluctuation and interactions of membrane proteins in the living cells of plants. Owing to the single-molecular level sensitivity and minimally invasive of FCS/FCCS, their application provides an ideal approach to understanding plant cell membrane lateral organization.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"2 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000220/pdfft?md5=8b585007e241f47dc6d0734582e845ea&pid=1-s2.0-S2949952624000220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141639421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-06-02DOI: 10.1016/j.ncrops.2024.100030
Jiaxuan Sui, Huiyu Tian, Zhaojun Ding, Xiangpei Kong
{"title":"Crop designs: The ideal root architecture for future crop breeding","authors":"Jiaxuan Sui, Huiyu Tian, Zhaojun Ding, Xiangpei Kong","doi":"10.1016/j.ncrops.2024.100030","DOIUrl":"10.1016/j.ncrops.2024.100030","url":null,"abstract":"<div><p>Root system architecture, a crucial agronomic trait for sustainable crop production, is influenced by a variety of internal developmental signals and external environmental factors. In this review, we highlight recent advancements in understanding the molecular mechanisms behind root meristem maintenance, cell differentiation, lateral root growth, root hair development, and crown root formation. Additionally, we explore how abiotic stresses such as drought, salinity, nitrate deficiency, and aluminum toxicity impact root system architecture. We identify key target genes that regulate root system architecture, offering potential targets for genome editing in future crop improvement. Finally, we discuss the opportunities and challenges in the de novo design of root system architecture.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000207/pdfft?md5=086c47e179140628b09e0ebd89ca5280&pid=1-s2.0-S2949952624000207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141277661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-05-24DOI: 10.1016/j.ncrops.2024.100029
Zhenghua He , Pei Zhang , Haitao Jia , Shilong Zhang , Elsayed Nishawy , Xiaopeng Sun , Mingqiu Dai
{"title":"Regulatory mechanisms and breeding strategies for crop drought resistance","authors":"Zhenghua He , Pei Zhang , Haitao Jia , Shilong Zhang , Elsayed Nishawy , Xiaopeng Sun , Mingqiu Dai","doi":"10.1016/j.ncrops.2024.100029","DOIUrl":"10.1016/j.ncrops.2024.100029","url":null,"abstract":"<div><p>Drought is a primary abiotic stress affecting crops, leading to plant stomatal closure, reduced photosynthetic capacity, and reduced yields or even harvest failure. Severe drought can adversely impact agricultural production, ecosystems, and socio-economic capacities. Recently, researchers have studied the regulatory mechanisms of crop drought resistance and cloned hundreds of genes via genetic and molecular approaches. However, a limited number of the cloned genes have been successfully employed in drought resistance breeding, suggesting that drought resistance regulation is too complex. More work must be done to fully understand the regulatory networks of drought responses to breed drought-resistant and high-yield crop varieties. This review outlines the current achievements in investigating crop drought responses, particularly regulation by phytohormones and regulation of genes at transcriptional, post-translational, and epigenetic levels in crop drought responses. Finally, we examine the problems and potential solutions in breeding crop drought resistance and propose strategies for crop drought resistance improvement.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100029"},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000190/pdfft?md5=100a64d1e88f2d816a46487d1f5a5a0c&pid=1-s2.0-S2949952624000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141140108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The hormonal, metabolic, and environmental regulation of plant shoot branching","authors":"Yuqi Liu , Shangyu Chen , Sikander Pal , Jingquan Yu , Yanhong Zhou , Lam-Son Phan Tran , Xiaojian Xia","doi":"10.1016/j.ncrops.2024.100028","DOIUrl":"10.1016/j.ncrops.2024.100028","url":null,"abstract":"<div><p>Plants have evolved varied structures for environmental adaptation. Shoot branching, as a part of plant architecture, influences the allocation of sugars produced by photosynthesis and thus greatly impacts crop yields. The activity of axillary meristem- and apical dominance govern- the shoot branching patterns. In this review, we summarize the key factors involved in the formation of lateral branches, and the mechanisms of how these factors are interconnected. In particular, we focus on recent advances in understanding how sugar and environmental signals affect the hormonal signaling network to regulate apical dominance. Ultimately, we propose that epigenetic modifications are critical mechanisms underlying the plasticity of shoot branching, and that precise targeted gene editing is promising for shaping the ideal plant architecture.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000189/pdfft?md5=441743d794ceb99b3532c8d1473ad79f&pid=1-s2.0-S2949952624000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141139040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-05-18DOI: 10.1016/j.ncrops.2024.100027
Zilu Zhang , Qi Wang , Haiqiao Yan , Xiaoyan Cang , Wei Li , Jinyu He , Meixiang Zhang , Laiqing Lou , Ran Wang , Ming Chang
{"title":"Lighting-up Wars: Stories of Ca2+ Signaling in Plant Immunity","authors":"Zilu Zhang , Qi Wang , Haiqiao Yan , Xiaoyan Cang , Wei Li , Jinyu He , Meixiang Zhang , Laiqing Lou , Ran Wang , Ming Chang","doi":"10.1016/j.ncrops.2024.100027","DOIUrl":"10.1016/j.ncrops.2024.100027","url":null,"abstract":"<div><p>Calcium ions (Ca<sup>2+</sup>) serve as key messengers in plant immune reactions. A typical Ca<sup>2+</sup> signaling involves three steps: encoding specific Ca<sup>2+</sup> signatures by Ca<sup>2+</sup>-permeable channels, decoding Ca<sup>2+</sup> signals by Ca<sup>2+</sup> sensors, and downstream responses. This review focuses on plasma membrane-localized Ca<sup>2+</sup>-permeable channels and cytosolic Ca<sup>2+</sup> sensors, unraveling their roles in cytosolic Ca<sup>2+</sup> influx and immune signaling during pattern-triggered immunity, effector-triggered immunity, and autoimmunity. Several unresolved questions were highlighted, including the regulation of Ca<sup>2+</sup>-permeable channel activity for immune induction and the mechanism behind Ca<sup>2+</sup> influx-triggered hypersensitive response cell death. This concise overview provides insights into the complex interplay of Ca<sup>2+</sup> signaling in plant immunity, paving the way for future investigations on molecular plant-microbe interactions.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100027"},"PeriodicalIF":0.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000177/pdfft?md5=9db07903da1969d0331b5713a86b749e&pid=1-s2.0-S2949952624000177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141139432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-05-16DOI: 10.1016/j.ncrops.2024.100026
Panting Fan , Jingjing Zhang , Lefei Gao , Mingke Wang , Hui Kong , Shengbo He
{"title":"Exploring the frontier of plant phase separation: Current insights and future prospects","authors":"Panting Fan , Jingjing Zhang , Lefei Gao , Mingke Wang , Hui Kong , Shengbo He","doi":"10.1016/j.ncrops.2024.100026","DOIUrl":"10.1016/j.ncrops.2024.100026","url":null,"abstract":"<div><p>In the rapidly evolving field of biology, phase separation has recently emerged as a revolutionary perspective, shedding new light on our comprehension of cellular processes. This review provides a comprehensive overview of current knowledge regarding phase separation in plants and charts promising avenues for future exploration. We delve into the fundamental principles of plant phase separation, highlighting the roles played by intrinsically disordered regions and prion-like domains. Summarizing significant advancements, we explore the involvement of phase separation in plant responses to environmental cues, as well as its involvement in growth and developmental processes, and plant-microbe interactions. Additionally, we present a streamlined workflow designed to guide the scientific community in conducting phase separation studies in plants. Lastly, we delineate lingering questions and propose potential applications of phase separation in agriculture.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000165/pdfft?md5=450d688aecc55835440c2b0c311ad113&pid=1-s2.0-S2949952624000165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141031369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-05-12DOI: 10.1016/j.ncrops.2024.100025
Qing Sang, Fanjiang Kong
{"title":"Applications for single-cell and spatial transcriptomics in plant research","authors":"Qing Sang, Fanjiang Kong","doi":"10.1016/j.ncrops.2024.100025","DOIUrl":"10.1016/j.ncrops.2024.100025","url":null,"abstract":"<div><p>Cells of multicellular plants possess inherent heterogeneity. Recent progress in single-cell RNA sequencing (scRNA-seq) allows researchers to classify, characterize, and distinguish individual cells at the transcriptome level, enabling the identification of rare cell populations with functional importance. However, scRNA-seq obscures spatial information about cells. Spatial transcriptomics approaches have substantially improved our capacity to detect the spatial distribution of RNA transcripts throughout tissues, yet it remains challenging to characterize whole-transcriptome-level data for single cells spatially. In this review, we offer a concise overview of the scRNA-seq and spatial transcriptomics experimental and computational procedures and the computational strategies required to integrate scRNA-seq data with spatial transcriptomics. We demonstrate their impact on plant fundamental cell biology, discuss their advantages and current challenges, and provide an outlook on the future.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000153/pdfft?md5=53f6d08fd117f9362e2da0bb22be2885&pid=1-s2.0-S2949952624000153-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141031444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-05-06DOI: 10.1016/j.ncrops.2024.100024
Limin Wang , Yanyan Wang , Pan Yin , Caifu Jiang , Ming Zhang
{"title":"ZmHAK17 encodes a Na+-selective transporter that promotes maize seed germination under salt conditions","authors":"Limin Wang , Yanyan Wang , Pan Yin , Caifu Jiang , Ming Zhang","doi":"10.1016/j.ncrops.2024.100024","DOIUrl":"10.1016/j.ncrops.2024.100024","url":null,"abstract":"<div><p>Elevated sodium ion (Na<sup>+</sup>) in saline farmlands adversely affect crops, notably by inhibiting seed germination. Given the importance of High-Affinity K<sup>+</sup> Transporter (HAK) family Na<sup>+</sup> transporters in plant salt tolerance and ZmHAK4 role in maize salt tolerance, our study focuses on characterizing HAK transporters related to ZmHAK4 in maize’s salt stress response. We found that ZmHAK17 is vital for promoting seed germination under saline conditions. Expressed mainly in the germinating embryo’s radicle, <em>ZmHAK17</em>, encodes a Na<sup>+</sup> transporter located in the plasma membrane. Increased <em>ZmHAK17</em> transcript levels under salt stress facilitate Na<sup>+</sup> efflux from the radicle, preventing Na<sup>+</sup> accumulation in the embryo and reducing salt stress effects on germination. Mutants lacking <em>ZmHAK17</em> exhibit salt-sensitive germination. This study highlights ZmHAK17 as a key transporter enhancing maize germination in saline conditions, deepening our understanding of HAK family Na<sup>+</sup> transporters’ role in salt tolerance and offering a new target gene for salt-tolerant maize breeding.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100024"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000141/pdfft?md5=34fee71255f81fe88034efc34f67d73b&pid=1-s2.0-S2949952624000141-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A necessary considering factor for crop resistance: Precise regulation and effective utilization of beneficial microorganisms","authors":"Chenxi Kou , Feiyang Song , Dandan Li, Hongyang Xu, Shuxin Zhang, Wei Yang, Wenchong Shi, Zheng Gao","doi":"10.1016/j.ncrops.2024.100023","DOIUrl":"10.1016/j.ncrops.2024.100023","url":null,"abstract":"<div><p>Under global climate change circumstances, there has been growing acknowledgment of the critical need to prevent and manage both biotic and abiotic stress in crops. Plants employ a variety of signaling molecules, such as jasmonic acid (JA), abscisic acid (ABA), and Ca<sup>2+</sup>, to endure stress. Additionally, they utilize reactive oxygen species (ROS) and detoxifiers while also harnessing the assistance of beneficial microorganisms, including <em>Bacillus, Pseudomonas</em>, <em>Streptomyces</em>, and others. These microorganisms play a pivotal role in aiding crops in stress management, albeit with certain limitations to their efficacy. This article offers a synthesis and discourse on how crops can selectively engage with beneficial microorganisms via their root systems, elucidating the contributions of these microorganisms to stress tolerance. Drawing upon current insights, we propose refined strategies for leveraging microorganisms to bolster crop stress resilience. By advancing our grasp of plant-microorganism interactions and judiciously selecting and employing beneficial microorganisms, we aim to enhance the dependability and efficacy of microbial products in enhancing crop stress tolerance. This knowledge ultimately equips agricultural practitioners with the information needed to make informed decisions and bolster crops in adapting to rapidly changing environmental conditions.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100023"},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S294995262400013X/pdfft?md5=95ff58f5f3db7604854c0bcc82271cde&pid=1-s2.0-S294995262400013X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140783161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
New CropsPub Date : 2024-03-30DOI: 10.1016/j.ncrops.2024.100021
Yueyuan Wang, Pan Li, Wan Sun, Tian Zhang
{"title":"Plant cell walls: Emerging targets of stomata engineering to improve photosynthesis and water use efficiency","authors":"Yueyuan Wang, Pan Li, Wan Sun, Tian Zhang","doi":"10.1016/j.ncrops.2024.100021","DOIUrl":"10.1016/j.ncrops.2024.100021","url":null,"abstract":"<div><p>Stomata are tiny pores on leaf surfaces essential for plant transpiration and photosynthesis. As gatekeepers that mediate gas exchange between plants and the atmosphere, stomata exert a major influence on global carbon and water cycles. The shape and function of stomata are physically constrained by stomatal walls. Compared to the extensively studied genetic mechanisms of stomatal development and guard cell signaling, recent progress is only beginning to uncover the role of plant cell walls in stomatal development and dynamics. In this review, we summarize the research on cell walls of the kidney-shaped stomata from dicots and the dumbbell-shaped stomata from grasses. As the dynamic response of grass stomata is closely linked to its anatomical features that are limited by cell walls, we discuss the potential of plant cell walls as crucial targets for crop engineering to enhance carbon assimilation and water use efficiency.</p></div>","PeriodicalId":100953,"journal":{"name":"New Crops","volume":"1 ","pages":"Article 100021"},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949952624000116/pdfft?md5=3e214ffd4d9c15f7f0c94d71378886e7&pid=1-s2.0-S2949952624000116-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140404104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}