Mechanobiology in Medicine最新文献_第3页

Tug of war: Understanding the dynamic interplay of tumor biomechanical environment on dendritic cell function 拔河比赛：了解肿瘤生物力学环境对树突状细胞功能的动态影响

Mechanobiology in Medicine Pub Date : 2024-04-27 DOI: 10.1016/j.mbm.2024.100068

Brian Chesney Quartey , Gabriella Torres , Mei ElGindi , Aseel Alatoom , Jiranuwat Sapudom , Jeremy CM Teo

{"title":"Tug of war: Understanding the dynamic interplay of tumor biomechanical environment on dendritic cell function","authors":"Brian Chesney Quartey , Gabriella Torres , Mei ElGindi , Aseel Alatoom , Jiranuwat Sapudom , Jeremy CM Teo","doi":"10.1016/j.mbm.2024.100068","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100068","url":null,"abstract":"<div><p>Dendritic cells (DCs) play a pivotal role in bridging the innate and adaptive immune systems. From their immature state, scavenging tissue for foreign antigens to uptake, then maturation, to their trafficking to lymph nodes for antigen presentation, these cells are exposed to various forms of mechanical forces. Particularly, in the tumor microenvironment, it is widely known that microenvironmental biomechanical cues are heightened. The source of these forces arises from cell-to-extracellular matrix (ECM) and cell-to-cell interactions, as well as being exposed to increased microenvironmental pressures and fluid shear forces typical of tumors. DCs then integrate these forces, influencing their immune functions through mechanotransduction. This aspect of DC biology holds alternative, but important clues to understanding suppressed/altered DC responses in tumors, or allow the artificial enhancement of DCs for therapeutic purposes. This review discusses the current understanding of DC mechanobiology from the perspectives of DCs as sensors of mechanical forces and providers of mechanical forces.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 3","pages":"Article 100068"},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000317/pdfft?md5=29269c7dc3e815e4976547bdca32e66d&pid=1-s2.0-S2949907024000317-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140824693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The motor-clutch model in mechanobiology and mechanomedicine 机械生物学和机械医学中的电机离合器模型

Mechanobiology in Medicine Pub Date : 2024-04-03 DOI: 10.1016/j.mbm.2024.100067

Zhao Xu , Feng Xu , Bo Cheng

引用次数: 0

Advances in micropatterning technology for mechanotransduction research 用于机械传导研究的微图案技术进展

Mechanobiology in Medicine Pub Date : 2024-03-28 DOI: 10.1016/j.mbm.2024.100066

Xinyu Hu , Min Bao

{"title":"Advances in micropatterning technology for mechanotransduction research","authors":"Xinyu Hu , Min Bao","doi":"10.1016/j.mbm.2024.100066","DOIUrl":"10.1016/j.mbm.2024.100066","url":null,"abstract":"<div><p>Micropatterning is a sophisticated technique that precisely manipulates the spatial distribution of cell adhesion proteins on various substrates across multiple scales. This precise control over adhesive regions facilitates the manipulation of architectures and physical constraints for single or multiple cells. Furthermore, it allows for an in-depth analysis of how chemical and physical properties influence cellular functionality. In this comprehensive review, we explore the current understanding of the impact of geometrical confinement on cellular functions across various dimensions, emphasizing the benefits of micropatterning in addressing fundamental biological queries. We advocate that utilizing directed self-organization via physical confinement and morphogen gradients on micropatterned surfaces represents an innovative approach to generating functional tissue and controlling morphogenesis in vitro. Integrating this technique with cutting-edge technologies, micropatterning presents a significant potential to bridge a crucial knowledge gap in understanding core biological processes.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 3","pages":"Article 100066"},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000299/pdfft?md5=0c38744e2d4e1a7de711ec2c0ce1dd83&pid=1-s2.0-S2949907024000299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140405018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roles of extracellular vesicles released by mechanically stimulated osteocytes in regulating osteoblast and osteoclast functions 机械刺激骨细胞释放的细胞外小泡在调控成骨细胞和破骨细胞功能中的作用

Mechanobiology in Medicine Pub Date : 2024-03-27 DOI: 10.1016/j.mbm.2024.100065

Yumei Chen , Runze Zhao , Li Yang , X. Edward Guo

{"title":"The roles of extracellular vesicles released by mechanically stimulated osteocytes in regulating osteoblast and osteoclast functions","authors":"Yumei Chen , Runze Zhao , Li Yang , X. Edward Guo","doi":"10.1016/j.mbm.2024.100065","DOIUrl":"10.1016/j.mbm.2024.100065","url":null,"abstract":"<div><p>Bone adapts to mechanical loading by changing its shape and mass. Osteocytes, as major mechanosensors, are critical for bone modeling/remodeling in response to mechanical stimuli. Intracellular calcium oscillation is one of the early responses in osteocytes, and this further facilitates bone cell communication through released biochemical signals. Our previous study has found that mechanically induced calcium oscillations in osteocytes enhance the release of extracellular vesicles (EVs), and those released EVs can elevate bone formation activity. However, the mechanism of mechanically stimulated EVs’ regulation of bone formation and resorption is still unclear. Here, using <em>in vitro</em> studies, we exposed OCY454 cells, with relatively high sclerostin expression, to steady fluid flow (SFF) and characterized the functions of rapidly released EVs in osteoblast and osteoclast regulation. Our study demonstrates that SFF stimulates intracellular calcium response in OCY454 cells and further induces sclerostin, osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL) inside or outside EVs to regulate osteoblast and osteoclast activities. This load-induced protein and EVs release is load-duration dependent. Moreover, stimulated osteocytes rapidly regulate osteoclast maturation through EVs capsulated RANKL. In contrast, other regulating proteins, OPG, and sclerostin, are mainly released directly into the medium without EV capsulation.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100065"},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000287/pdfft?md5=e2faf11abd74c6b1e6ba230c7caae064&pid=1-s2.0-S2949907024000287-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Force-dependent rapid immunoassay of high specificity and sensitivity 高特异性和高灵敏度的力依赖性快速免疫分析法

Mechanobiology in Medicine Pub Date : 2024-03-21 DOI: 10.1016/j.mbm.2024.100061

Xiaodan Zhao , Yanqige Jiang , Yu Zhou , Jie Yan

引用次数: 0

Probing the protrusions: lamellipodia and filopodia in cancer invasion and beyond 探究突起：癌症侵袭及其他过程中的片状和丝状突起

Mechanobiology in Medicine Pub Date : 2024-03-20 DOI: 10.1016/j.mbm.2024.100064

Laras Pratiwi, Elisa Elisa, Henry Sutanto

{"title":"Probing the protrusions: lamellipodia and filopodia in cancer invasion and beyond","authors":"Laras Pratiwi, Elisa Elisa, Henry Sutanto","doi":"10.1016/j.mbm.2024.100064","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100064","url":null,"abstract":"<div><p>The dynamic protrusions of lamellipodia and filopodia have emerged as crucial players in tumor progression and metastasis. These membrane structures, governed by intricate actin cytoskeletal rearrangements, facilitate cancer cell migration, invasion, and interaction with the tumor microenvironment. This review provides a comprehensive examination of the structural and functional attributes of lamellipodia and filopodia, shedding light on their pivotal roles in mediating cancer invasion. Navigating through the intricate landscape of cancer biology, the review illuminates the intricate signaling pathways and regulatory mechanisms orchestrating the formation and activity of these protrusions. The discussion extends to the clinical implications of lamellipodia and filopodia, exploring their potential as diagnostic and prognostic markers, and delving into therapeutic strategies that target these structures to impede cancer progression. As we delve into the future, the review outlines emerging technologies and unexplored facets that beckon further research, emphasizing the need for collaborative efforts to unravel the complexities of lamellipodia and filopodia in cancer, ultimately paving the way for innovative therapeutic interventions.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100064"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000275/pdfft?md5=5e8e0610146b04739a39d45afef98e3d&pid=1-s2.0-S2949907024000275-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140209064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular matrix stiffness modulates the mechanophenotypes and focal adhesions of colon cancer cells leading to their invasions via YAP1 细胞外基质硬度通过 YAP1 调节结肠癌细胞的机械表型和病灶粘附，从而导致其侵袭

Mechanobiology in Medicine Pub Date : 2024-03-19 DOI: 10.1016/j.mbm.2024.100062

Kaide Xia , Wenhui Hu , Yun Wang , Jin Chen , Zuquan Hu , Chenyi An , Pu Xu , Lijing Teng , Jieheng Wu , Lina Liu , Sichao Zhang , Jinhua Long , Zhu Zeng

{"title":"Extracellular matrix stiffness modulates the mechanophenotypes and focal adhesions of colon cancer cells leading to their invasions via YAP1","authors":"Kaide Xia , Wenhui Hu , Yun Wang , Jin Chen , Zuquan Hu , Chenyi An , Pu Xu , Lijing Teng , Jieheng Wu , Lina Liu , Sichao Zhang , Jinhua Long , Zhu Zeng","doi":"10.1016/j.mbm.2024.100062","DOIUrl":"10.1016/j.mbm.2024.100062","url":null,"abstract":"<div><p>Distal metastasis is the main cause of clinical treatment failure in patients with colon cancer. It is now known that the invasion and metastasis of cancer cells is precisely regulated by chemical and physical factors <em>in vivo</em>. However, the role of extracellular matrix (ECM) stiffness in colon cancer cell (CCCs) invasion and metastasis remains unclear. Here, bioinformatical analysis suggested that a high expression level of yes associated protein 1 (YAP1) was significantly associated with metastasis and poor prognosis in colon cancer patients. We further investigated the effects of polyacrylamide hydrogels with different stiffnesses (3, 20, and 38 kPa), which were simulated as ECM, on the mechanophenotype (F-actin cytoskeleton organization, electrophoretic rate, membrane fluidity, and Young's modulus) of CCCs. The results showed that a stiffer ECM could induce the maturation of focal adhesions and formation of stress fibers in CCCs, regulate their mechanophenotypes, and promote cell motility. We also demonstrated that the expression levels of YAP1 and paxillin were positively correlated in patients with colon cancer. YAP1 knockdown reduces paxillin clustering and cell motility and alters the cellular mechanophenotypes of CCCs. This is of great significance for an in-depth understanding of the invasion and metastatic mechanisms of colon cancer and for the optimization of clinical therapy from the perspective of mechanobiology.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100062"},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000251/pdfft?md5=8225712552eb8e5633ae7b59efe64ce1&pid=1-s2.0-S2949907024000251-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140280551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment 基于微珠的微流控平台，用于临床诊断和治疗中的多重和超灵敏免疫测定

Mechanobiology in Medicine Pub Date : 2024-03-16 DOI: 10.1016/j.mbm.2024.100063

Xiaoxia Fang, Yiwen Yang, Heni Wang, Hong Xu

引用次数: 0

Regulatory role of interfacial adhesion and mechanical microenvironments in microbe-host interactions 界面粘附和机械微环境在微生物-宿主相互作用中的调节作用

Mechanobiology in Medicine Pub Date : 2024-03-01 DOI: 10.1016/j.mbm.2024.100060

Yuting Feng, Jianyong Huang

{"title":"Regulatory role of interfacial adhesion and mechanical microenvironments in microbe-host interactions","authors":"Yuting Feng, Jianyong Huang","doi":"10.1016/j.mbm.2024.100060","DOIUrl":"10.1016/j.mbm.2024.100060","url":null,"abstract":"<div><p>A recent study published in <em>Nature Communications</em> showed that essential modulatory roles of interfacial adhesion and mechanical microenvironments such as geometric constraints and extracellular matrix stiffness, in microbe-host cell interactions. This study utilized single-cell force spectroscopy and RNA sequencing to gain insight into the intrinsic mechanisms by which the mechanical microenvironment regulates bacterial-host interactions and therefore reveal potential interventions against bacterial invasion. Meanwhile, the adhesion forces involved in the bacterial–host interactions were recognized as a new indicator for assessing the extent of bacterial infection. Taken together, these findings demonstrate that interfacial adhesion forces and mechanical microenvironments play a dominant role in modulating functions and behaviors of microorganisms and host cells, which also provide a mechanobiology-inspired idea for the development of subsequent drug-resistant antimicrobials and broad-spectrum antiviral drugs.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000238/pdfft?md5=5a9dc4292dc1bffe61407088d51445eb&pid=1-s2.0-S2949907024000238-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rescuing SERCA2 pump deficiency: A novel approach to improve bone mechano-responsiveness in type 2 diabetes 挽救 SERCA2 泵缺陷：改善 2 型糖尿病患者骨骼机械反应性的新方法

Mechanobiology in Medicine Pub Date : 2024-02-24 DOI: 10.1016/j.mbm.2024.100047

Zhifeng Yu , X. Edward Guo

{"title":"Rescuing SERCA2 pump deficiency: A novel approach to improve bone mechano-responsiveness in type 2 diabetes","authors":"Zhifeng Yu , X. Edward Guo","doi":"10.1016/j.mbm.2024.100047","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100047","url":null,"abstract":"<div><p>A recent study published in <em>Nature Communications</em> demonstrated that restoring SERCA2 pump deficiency can enhance bone mechano-responsiveness in type 2 diabetes (T2D) by modulating osteocyte calcium dynamics. The findings revealed that in T2D mice, the ability of the bone to respond to mechanical stress is compromised, primarily due to attenuated calcium oscillatory dynamics within osteocytes rather than in osteoblasts or osteoclasts. The underlying mechanism of this reduction in bone mechano-responsiveness in T2D was identified as a specific decrease in osteocytic SERCA2 expression mediated by PPARα. Additionally, mice overexpressing SERCA2 in osteocytes exhibited reduced deterioration of bone mechano-responsiveness induced by T2D. Collectively, this study provides mechanistic insights into T2D-induced deterioration in bone mechano-responsiveness and identifies a promising therapeutic approach to counteract T2D-associated fragility fractures.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100047"},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S294990702400010X/pdfft?md5=673fabd07f7fc63f32f1485c817369bc&pid=1-s2.0-S294990702400010X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0