Mechanobiology in Medicine最新文献_第2页

Mechanobiomaterials: Harnessing mechanobiology principles for tissue repair and regeneration 机械生物材料：利用机械生物学原理促进组织修复和再生

Mechanobiology in Medicine Pub Date : 2024-05-16 DOI: 10.1016/j.mbm.2024.100079

Xiao Lin , Hua Yang , Yi Xia , Kang Wu , Fengcheng Chu , Huan Zhou , Huajian Gao , Lei Yang

{"title":"Mechanobiomaterials: Harnessing mechanobiology principles for tissue repair and regeneration","authors":"Xiao Lin , Hua Yang , Yi Xia , Kang Wu , Fengcheng Chu , Huan Zhou , Huajian Gao , Lei Yang","doi":"10.1016/j.mbm.2024.100079","DOIUrl":"10.1016/j.mbm.2024.100079","url":null,"abstract":"<div><p>Mechanical stimuli are known to play critical roles in mediating tissue repair and regeneration. Recently, this knowledge has led to a paradigm shift toward proactive programming of biological functionalities of biomaterials by leveraging mechanics–geometry–biofunction relationships, which are beginning to shape the newly emerging field of mechanobiomaterials. To profile this emerging field, this article aims to elucidate the fundamental principles in modulating biological responses with material–tissue mechanical interactions, illustrate recent findings on the relationships between material properties and biological responses, discuss the importance of mathematical/physical models and numerical simulations in optimizing material properties and geometry, and outline design strategies for mechanobiomaterials and their potential for tissue repair and regeneration. Given that the field of mechanobiomaterials is still in its infancy, this article also discusses open questions and challenges that need to be addressed.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000421/pdfft?md5=320fe996100a6e4b52edd7711af259b4&pid=1-s2.0-S2949907024000421-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141041150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical force induced activation of adhesion G protein–coupled receptor 机械力诱导激活粘附 G 蛋白偶联受体

Mechanobiology in Medicine Pub Date : 2024-05-14 DOI: 10.1016/j.mbm.2024.100078

Yueming Xu , Huanhuan Xu , Jie Yan , Gaojie Song

{"title":"Mechanical force induced activation of adhesion G protein–coupled receptor","authors":"Yueming Xu , Huanhuan Xu , Jie Yan , Gaojie Song","doi":"10.1016/j.mbm.2024.100078","DOIUrl":"10.1016/j.mbm.2024.100078","url":null,"abstract":"<div><p>Among the various families of G protein-couple receptors (GPCR), the adhesion family of GPCRs is specialized by its expansive extracellular region, which facilitates the recruitment of various ligands. Previous hypothesis proposed that aGPCRs are activated by mechanical force, wherein a <em>Stachel</em> peptide is liberated from the GPCR autoproteolysis-inducing (GAIN) domain and subsequently binds to the transmembrane domain (7TM) upon activation. In this review, we summarize recent advancements in structural studies of aGPCRs, unveiling a conserved structural change of the <em>Stachel</em> peptide from the GAIN domain-embedded β-strand conformation to the 7TM-loaded α-helical conformation. Notably, using single-molecule studies, we directly observed the unfolding of GAIN domain and the release of <em>Stachel</em> peptide under physiological level of force, precisely supporting the mechanosensing mechanism for aGPCRs. We observed that the current complex structures of aGPCR adhesion domains with their respective ligands share a common pattern with the C-termini of each binding partner extending in opposite directions, suggesting a similar shearing stretch geometry for these aGPCRs to transmit the mechanical force generated in the circulating environment to the GAIN domain for its unfolding. Outstanding questions, including the relative orientations and interactions between 7TM and its preceding GAIN and adhesion domains of different aGPCRs, may require further structural and mechanical studies at the full-length receptor scale or cell-based level. Our analysis extends the current view of aGPCR structural organization and activation and offers valuable insights for the development of mechanosensor based on aGPCRs or discovery of mechanotherapy against aGPCRs.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S294990702400041X/pdfft?md5=bf52735e6165c1ef20cbbeb3f0ccd37a&pid=1-s2.0-S294990702400041X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric crowders and membrane morphology at the nexus of intracellular trafficking and oncology 细胞内运输与肿瘤学关系中的非对称拥挤器和膜形态学

Mechanobiology in Medicine Pub Date : 2024-05-03 DOI: 10.1016/j.mbm.2024.100071

Kshitiz Parihar , Seung-Hyun B. Ko , Ryan P. Bradley , Phillip Taylor , N. Ramakrishnan , Tobias Baumgart , Wei Guo , Valerie M. Weaver , Paul A. Janmey , Ravi Radhakrishnan

{"title":"Asymmetric crowders and membrane morphology at the nexus of intracellular trafficking and oncology","authors":"Kshitiz Parihar , Seung-Hyun B. Ko , Ryan P. Bradley , Phillip Taylor , N. Ramakrishnan , Tobias Baumgart , Wei Guo , Valerie M. Weaver , Paul A. Janmey , Ravi Radhakrishnan","doi":"10.1016/j.mbm.2024.100071","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100071","url":null,"abstract":"<div><p>A definitive understanding of the interplay between protein binding/migration and membrane curvature evolution is emerging but needs further study. The mechanisms defining such phenomena are critical to intracellular transport and trafficking of proteins. Among trafficking modalities, exosomes have drawn attention in cancer research as these nano-sized naturally occurring vehicles are implicated in intercellular communication in the tumor microenvironment, suppressing anti-tumor immunity and preparing the metastatic niche for progression. A significant question in the field is how the release and composition of tumor exosomes are regulated. In this perspective article, we explore how physical factors such as geometry and tissue mechanics regulate cell cortical tension to influence exosome production by co-opting the biophysics as well as the signaling dynamics of intracellular trafficking pathways and how these exosomes contribute to the suppression of anti-tumor immunity and promote metastasis. We describe a multiscale modeling approach whose impact goes beyond the fundamental investigation of specific cellular processes toward actual clinical translation. Exosomal mechanisms are critical to developing and approving liquid biopsy technologies, poised to transform future non-invasive, longitudinal profiling of evolving tumors and resistance to cancer therapies to bring us one step closer to the promise of personalized medicine.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000342/pdfft?md5=82f74f64557cd0e9b08dee1c94f412b1&pid=1-s2.0-S2949907024000342-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic investigation for shear-stress-mediated repair of dysglycemia-induced endothelial cell damage 剪切应力介导的血糖异常内皮细胞损伤修复微流体研究

Mechanobiology in Medicine Pub Date : 2024-04-29 DOI: 10.1016/j.mbm.2024.100069

Si-Yu Hu , Chun-Dong Xue , Yong-Jiang Li , Shen Li , Zheng-Nan Gao , Kai-Rong Qin

{"title":"Microfluidic investigation for shear-stress-mediated repair of dysglycemia-induced endothelial cell damage","authors":"Si-Yu Hu , Chun-Dong Xue , Yong-Jiang Li , Shen Li , Zheng-Nan Gao , Kai-Rong Qin","doi":"10.1016/j.mbm.2024.100069","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100069","url":null,"abstract":"<div><p>Dysglycemia causes arterial endothelial damage, which is an early critical event in vascular complications for diabetes patients. Physiologically, moderate shear stress (SS) helps maintain endothelial cell health and normal function. Reactive oxygen species (ROS) and calcium ions (Ca<sup>2+</sup>) signals are involved in dysglycemia-induced endothelial dysfunction and are also implicated in SS-mediated regulation of endothelial cell function. Therefore, it is urgent to establish <em>in vitro</em> models for studying endothelial biomechanics and mechanobiology, aiming to seek interventions that utilize appropriate SS to delay or reverse endothelial dysfunction. Microfluidic technology, as a novel approach, makes it possible to replicate blood glucose environment and accurate pulsatile SS <em>in vitro</em>. Here, we reviewed the progress of microfluidic systems used for SS-mediated repair of dysglycemia-induced endothelial cell damage (ECD), revealing the crucial roles of ROS and Ca<sup>2+</sup> during the processes. It holds significant implications for finding appropriate mechanical intervention methods, such as exercise training, to prevent and treat cardiovascular complications in diabetes.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000329/pdfft?md5=a518abb78cf0a5c00975a90358021bba&pid=1-s2.0-S2949907024000329-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable and oscillatory hypoxia differentially regulate invasibility of breast cancer associated fibroblasts 稳定型和振荡型缺氧可对乳腺癌相关成纤维细胞的侵袭性进行不同程度的调控

Mechanobiology in Medicine Pub Date : 2024-04-27 DOI: 10.1016/j.mbm.2024.100070

Wenqiang Du , Ashkan Novin , Yamin Liu , Junaid Afzal , Shaofei Liu , Yasir Suhail , Kshitiz

{"title":"Stable and oscillatory hypoxia differentially regulate invasibility of breast cancer associated fibroblasts","authors":"Wenqiang Du , Ashkan Novin , Yamin Liu , Junaid Afzal , Shaofei Liu , Yasir Suhail , Kshitiz","doi":"10.1016/j.mbm.2024.100070","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100070","url":null,"abstract":"<div><p>As local regions in the tumor outstrip their oxygen supply, hypoxia can develop, affecting not only the cancer cells, but also other cells in the microenvironment, including cancer associated fibroblasts (CAFs). Hypoxia is also not necessarily stable over time, and can fluctuate or oscillate. Hypoxia Inducible Factor-1 is the master regulator of cellular response to hypoxia, and can also exhibit oscillations in its activity. To understand how stable, and fluctuating hypoxia influence breast CAFs, we measured changes in gene expression in CAFs in normoxia, hypoxia, and oscillatory hypoxia, as well as measured change in their capacity to resist, or assist breast cancer invasion. We show that hypoxia has a profound effect on breast CAFs causing activation of key pathways associated with fibroblast activation, but reduce myofibroblast activation and traction force generation. We also found that oscillatory hypoxia, while expectedly resulted in a “sub-hypoxic” response in gene expression, it resulted in specific activation of pathways associated with actin polymerization and actomyosin maturation. Using traction force microscopy, and a nanopatterned stromal invasion assay, we show that oscillatory hypoxia increases contractile force generation vs stable hypoxia, and increases heterogeneity in force generation response, while also additively enhancing invasibility of CAFs to MDA-MB-231 invasion. Our data show that stable and unstable hypoxia can regulate many mechnobiological characteristics of CAFs, and can contribute to transformation of CAFs to assist cancer dissemination and onset of metastasis.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000330/pdfft?md5=3b091afca74e4a263697c398a9097d07&pid=1-s2.0-S2949907024000330-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tug of war: Understanding the dynamic interplay of tumor biomechanical environment on dendritic cell function 拔河比赛：了解肿瘤生物力学环境对树突状细胞功能的动态影响

Mechanobiology in Medicine Pub Date : 2024-04-27 DOI: 10.1016/j.mbm.2024.100068

Brian Chesney Quartey , Gabriella Torres , Mei ElGindi , Aseel Alatoom , Jiranuwat Sapudom , Jeremy CM Teo

{"title":"Tug of war: Understanding the dynamic interplay of tumor biomechanical environment on dendritic cell function","authors":"Brian Chesney Quartey , Gabriella Torres , Mei ElGindi , Aseel Alatoom , Jiranuwat Sapudom , Jeremy CM Teo","doi":"10.1016/j.mbm.2024.100068","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100068","url":null,"abstract":"<div><p>Dendritic cells (DCs) play a pivotal role in bridging the innate and adaptive immune systems. From their immature state, scavenging tissue for foreign antigens to uptake, then maturation, to their trafficking to lymph nodes for antigen presentation, these cells are exposed to various forms of mechanical forces. Particularly, in the tumor microenvironment, it is widely known that microenvironmental biomechanical cues are heightened. The source of these forces arises from cell-to-extracellular matrix (ECM) and cell-to-cell interactions, as well as being exposed to increased microenvironmental pressures and fluid shear forces typical of tumors. DCs then integrate these forces, influencing their immune functions through mechanotransduction. This aspect of DC biology holds alternative, but important clues to understanding suppressed/altered DC responses in tumors, or allow the artificial enhancement of DCs for therapeutic purposes. This review discusses the current understanding of DC mechanobiology from the perspectives of DCs as sensors of mechanical forces and providers of mechanical forces.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000317/pdfft?md5=29269c7dc3e815e4976547bdca32e66d&pid=1-s2.0-S2949907024000317-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140824693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The motor-clutch model in mechanobiology and mechanomedicine 机械生物学和机械医学中的电机离合器模型

Mechanobiology in Medicine Pub Date : 2024-04-03 DOI: 10.1016/j.mbm.2024.100067

Zhao Xu , Feng Xu , Bo Cheng

引用次数: 0

Advances in micropatterning technology for mechanotransduction research 用于机械传导研究的微图案技术进展

Mechanobiology in Medicine Pub Date : 2024-03-28 DOI: 10.1016/j.mbm.2024.100066

Xinyu Hu , Min Bao

{"title":"Advances in micropatterning technology for mechanotransduction research","authors":"Xinyu Hu , Min Bao","doi":"10.1016/j.mbm.2024.100066","DOIUrl":"10.1016/j.mbm.2024.100066","url":null,"abstract":"<div><p>Micropatterning is a sophisticated technique that precisely manipulates the spatial distribution of cell adhesion proteins on various substrates across multiple scales. This precise control over adhesive regions facilitates the manipulation of architectures and physical constraints for single or multiple cells. Furthermore, it allows for an in-depth analysis of how chemical and physical properties influence cellular functionality. In this comprehensive review, we explore the current understanding of the impact of geometrical confinement on cellular functions across various dimensions, emphasizing the benefits of micropatterning in addressing fundamental biological queries. We advocate that utilizing directed self-organization via physical confinement and morphogen gradients on micropatterned surfaces represents an innovative approach to generating functional tissue and controlling morphogenesis in vitro. Integrating this technique with cutting-edge technologies, micropatterning presents a significant potential to bridge a crucial knowledge gap in understanding core biological processes.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000299/pdfft?md5=0c38744e2d4e1a7de711ec2c0ce1dd83&pid=1-s2.0-S2949907024000299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140405018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roles of extracellular vesicles released by mechanically stimulated osteocytes in regulating osteoblast and osteoclast functions 机械刺激骨细胞释放的细胞外小泡在调控成骨细胞和破骨细胞功能中的作用

Mechanobiology in Medicine Pub Date : 2024-03-27 DOI: 10.1016/j.mbm.2024.100065

Yumei Chen , Runze Zhao , Li Yang , X. Edward Guo

{"title":"The roles of extracellular vesicles released by mechanically stimulated osteocytes in regulating osteoblast and osteoclast functions","authors":"Yumei Chen , Runze Zhao , Li Yang , X. Edward Guo","doi":"10.1016/j.mbm.2024.100065","DOIUrl":"10.1016/j.mbm.2024.100065","url":null,"abstract":"<div><p>Bone adapts to mechanical loading by changing its shape and mass. Osteocytes, as major mechanosensors, are critical for bone modeling/remodeling in response to mechanical stimuli. Intracellular calcium oscillation is one of the early responses in osteocytes, and this further facilitates bone cell communication through released biochemical signals. Our previous study has found that mechanically induced calcium oscillations in osteocytes enhance the release of extracellular vesicles (EVs), and those released EVs can elevate bone formation activity. However, the mechanism of mechanically stimulated EVs’ regulation of bone formation and resorption is still unclear. Here, using <em>in vitro</em> studies, we exposed OCY454 cells, with relatively high sclerostin expression, to steady fluid flow (SFF) and characterized the functions of rapidly released EVs in osteoblast and osteoclast regulation. Our study demonstrates that SFF stimulates intracellular calcium response in OCY454 cells and further induces sclerostin, osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL) inside or outside EVs to regulate osteoblast and osteoclast activities. This load-induced protein and EVs release is load-duration dependent. Moreover, stimulated osteocytes rapidly regulate osteoclast maturation through EVs capsulated RANKL. In contrast, other regulating proteins, OPG, and sclerostin, are mainly released directly into the medium without EV capsulation.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000287/pdfft?md5=e2faf11abd74c6b1e6ba230c7caae064&pid=1-s2.0-S2949907024000287-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Force-dependent rapid immunoassay of high specificity and sensitivity 高特异性和高灵敏度的力依赖性快速免疫分析法

Mechanobiology in Medicine Pub Date : 2024-03-21 DOI: 10.1016/j.mbm.2024.100061

Xiaodan Zhao , Yanqige Jiang , Yu Zhou , Jie Yan

引用次数: 0