iLABMED最新文献

{"title":"Diagnostic approaches for monkeypox virus","authors":"Guohao Fan,&nbsp;Jiahua Kuang,&nbsp;Shengjie Zhang,&nbsp;Yang Yang,&nbsp;Yingxia Liu,&nbsp;Hongzhou Lu","doi":"10.1002/ila2.36","DOIUrl":"10.1002/ila2.36","url":null,"abstract":"<p>Mpox (formerly Monkeypox) is a zoonotic infection caused by Monkeypox virus (MPXV). Since 2022, Mpox epidemics have occurred in many non-endemic countries and regions, leading the World Health Organization to declare a public health emergency of international concern. With the persistent transmission and evolution of MPXV, symptoms of Mpox have become milder, with some infections being asymptomatic. In addition, MPXV has become more contagious. Therefore, rapid and accurate diagnosis and screening of MPXV is vital to prevent and control MPXV epidemics. Here, we review and summarize the technical details, application scenarios, and the advantages and disadvantages of MPXV-specific diagnostic methods.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"6-13"},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140259101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From bench to bedside: Opportunities and challenges for iLABMED","authors":"Tetsuya Asakawa,&nbsp;Yi-Wei Tang,&nbsp;Hongzhou Lu","doi":"10.1002/ila2.33","DOIUrl":"https://doi.org/10.1002/ila2.33","url":null,"abstract":"<p>Since the new <i>iLABMED</i> was founded in June 2023, it has published three issues with 21 articles (till Dec 2023). As a journal majored in laboratory medicine, <i>iLABMED</i> also has to face many opportunities and challenges of laboratory medicine. This editorial summarized the main opportunities and challenges faced by <i>iLABMED</i>. The future prospects of laboratory medicine, which must be highlighted by <i>iLABMED</i> were also discussed based on a brief review of the current advances in laboratory medicine. <i>iLABMED</i> will continue to provide a useful platform for general-interested, insightful, and informative articles with high quality.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140291380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa infections and improper storage conditions influence the performance of 1,3-β-d-glucan in diagnosis of invasive fungal infections","authors":"Zilan Wei,&nbsp;Jie Xu,&nbsp;Fang Yuan,&nbsp;Wendong Fang,&nbsp;Jiahui Wu,&nbsp;Youliang Wang,&nbsp;Shuiping Chen","doi":"10.1002/ila2.35","DOIUrl":"https://doi.org/10.1002/ila2.35","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The association between 1,3-<i>β</i>-<span>d</span>-glucan (BDG) levels and infections caused by <i>Pseudomonas aeruginosa</i> or <i>Streptococcus pneumoniae</i>, and the stability of BDG under different storage conditions are unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Strains of <i>Pseudomonas aeruginosa</i> and <i>S</i>. <i>pneumoniae</i> were grown in medium and human serum. The BDG concentrations in culture supernatants were measured. The specificity and stability of BDG were also evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>P</i>. <i>aeruginosa</i> produced high levels of BDG in Luria–Bertani medium (&gt;4 × 10⁴ pg/mL) and human serum (527.0 pg/mL), whereas <i>S</i>. <i>pneumoniae</i> produced low levels of BDG in THY medium (175.6 pg/mL) and human serum (78.3 pg/mL). The BDG produced by these two bacteria was specifically degraded by 1,3-<i>β</i>-<span>d</span>-glucanase. BDG was degraded when stored at different temperatures, decreasing by 22.5% and 9.3% at −20°C and −70°C, respectively, for 63 days; by 30.7% at 4°C for 12 days; and by 12.6% and 22.0% at 37°C for 6 and 12 h.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>BDG false-positivity must be considered in patients with bacteremia caused by <i>P</i>. <i>aeruginosa</i> when diagnosing invasive fungal infection. Human serum samples for the BDG test in medical facilities should be tested as soon as possible or stored at low temperatures before testing.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"53-59"},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140291358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers for predicting efficacy of chimeric antigen receptor T cell therapy and their detection methods","authors":"Bo Zou,&nbsp;Yanzhou Song,&nbsp;Ning Li,&nbsp;Zhongyi Fan,&nbsp;Jie Li,&nbsp;Yuanzheng Peng,&nbsp;Wanshan Wei,&nbsp;Yuzi Zhang,&nbsp;Yinan Su,&nbsp;Xianmin Meng,&nbsp;Hongzhou Lu,&nbsp;Xingding Zhang,&nbsp;Xiaohua Tan,&nbsp;Qibin Liao","doi":"10.1002/ila2.31","DOIUrl":"10.1002/ila2.31","url":null,"abstract":"<p>Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment, alongside surgery, radiotherapy, and chemotherapy. After several decades of development, chimeric antigen receptor T (CAR-T) cell therapy, a promising branch of adoptive T-cell therapy, has demonstrated superior efficacy and safety in comparison to other cell therapies in the treatment of cancer. At present, CAR-T cells are predominantly used to treat hematological malignancies, although their application in solid tumors is being readily investigated. Although numerous studies have examined the biomarkers associated with the safety of CAR-T cell therapy, few have evaluated predictors of CAR-T cell therapeutic efficacy. Thus, the primary objective of this review article was to provide a comprehensive overview of the factors predicting the efficacy of CAR-T cell therapy, with a particular focus on biomarkers and their detection methods.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"14-26"},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of new diagnostic targets for hepatitis B virus-induced liver fibrosis","authors":"Ying Wang,&nbsp;Shuo Qin,&nbsp;Meng Yang,&nbsp;Xiaoling Wang","doi":"10.1002/ila2.30","DOIUrl":"10.1002/ila2.30","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Liver fibrosis is a transitional stage from hepatitis to cirrhosis, and hepatitis B virus (HBV) is the most common cause of liver disease. Transcriptome sequencing technology and bioinformatics analysis are increasingly being used to screen diagnostic targets for liver fibrosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The GSE171294 dataset of HBV-induced liver fibrosis tissue and normal tissue was obtained from the Gene Expression Omnibus (GEO) public database and used to screen for differentially expressed mRNAs using R software. mRNAs with |log fold change| &gt;1 and <i>p</i> &lt; 0.05 were considered to be differentially expressed. A heat map was drawn to visualize the expression patterns of the differentially expressed mRNAs. To screen for candidate target mRNAs, the differentially expressed mRNAs were annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis. Finally, a protein–protein interaction (PPI) network was constructed to analyze the relationships between the differentially expressed mRNAs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 243 differentially expressed mRNAs were identified (<i>p</i> &lt; 0.05); 129 were up-regulated and 114 were down-regulated. The up-regulated and down-regulated mRNAs were significantly enriched in 16 and 8 KEGG pathways, respectively. The enriched KEGG pathways included Salmonella infection, Protein processing in the endoplasmic reticulum, IL-17 signaling pathway, and Aldosterone synthesis and secretion. The enriched GO terms were related mainly to cell proliferation, apoptosis, endoplasmic reticulum complex assembly, and myosin synthesis. The PPI network contained 161 nodes and 120 pairs of interactions. The top 10 key nodes were <i>CAV1, CD4, NR3C1, PDIA3, EZR, IRF4, SOX9, HSP90AB1, CD40,</i> and <i>SEC13</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Bioinformatics analysis of the transcriptome sequencing data in the GSE171294 dataset identified <i>CD4</i>, <i>NR3C1</i>, and <i>EZR</i> and other genes at key nodes as new targets for the treatment of liver fibrosis caused by HBV. These results provide new insights for HBV-induced liver fibrosis research and clinical treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"27-37"},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.30","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139453544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the epidemic characteristics of Mycoplasma pneumoniae infection around COVID pandemic","authors":"Hongmei Sun,&nbsp;Li Xiao","doi":"10.1002/ila2.27","DOIUrl":"https://doi.org/10.1002/ila2.27","url":null,"abstract":"<p><i>Mycoplasma pneumoniae</i> (<i>M. pneumoniae</i>) is a cell wall-less respiratory pathogen causing community-acquired pneumonia and extrapulmonary manifestations. It is transmitted through close contact and shows periodic regional outbreaks. The coronavirus disease (COVID-19) pandemic interfered with the global spread of <i>M. pneumoniae</i>. A large-scale post-COVID outbreak is currently ongoing in China. To help physicians better understand and manage this epidemic, we provide this review summarizing current knowledge on the pathogenesis, epidemic characteristics, macrolide resistance, diagnostic methods, and clinical treatment strategies for this pathogen.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"148-157"},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.27","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The important role of skin biopsies in the diagnosis of mpox","authors":"Wun-Ju Shieh","doi":"10.1002/ila2.29","DOIUrl":"10.1002/ila2.29","url":null,"abstract":"<p>Skin biopsy is an important, efficient, and safe procedure for diagnosing infectious dermatopathy, including mpox. An optimal approach of laboratory diagnosis of mpox should include obtaining a skin punch biopsy from pertinent lesions for pathologic evaluation in addition to conventional swab or scrap for molecular testing.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"143-147"},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138586130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutating Escherichia coli caused multiple organ dysfunction syndrome","authors":"Lihua Qi,&nbsp;Wencheng Xue,&nbsp;Xuelin Liu,&nbsp;Shaofu Qiu,&nbsp;Jie Liu","doi":"10.1002/ila2.26","DOIUrl":"10.1002/ila2.26","url":null,"abstract":"<p>Pathogenic <i>Escherichia coli</i> is of great concern in the clinical setting. But few reports have demonstrated the variation in disease course. We present a severe case of multiple organ dysfunction syndrome caused by <i>E. coli</i> infection. Pathogens isolated from blood and urine samples harboured many virulence factors. Whole-genome sequencing and conventional analyses showed that the isolates experienced beneficial variations, both genetically and phenotypically, during the disease course. These findings showed that <i>E. coli</i> can cause systemic symptoms and informed us of the importance of assessing the reasons for such variations in pathogens occurring in vivo.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"190-195"},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138585754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease","authors":"Xianzong Ma,&nbsp;Wenyu Zhang,&nbsp;Xin Wang,&nbsp;Lang Yang,&nbsp;Juan Jiao,&nbsp;Yan Jia,&nbsp;Dezhi Wang,&nbsp;Junfeng Xu,&nbsp;Peng Jin,&nbsp;Mingjie Zhang,&nbsp;Shirong Li,&nbsp;Yuanming Pan,&nbsp;Jianqiu Sheng","doi":"10.1002/ila2.28","DOIUrl":"10.1002/ila2.28","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The search for biomarkers suitable for early diagnosis of Crohn's disease (CD) is challenging. This study investigated the efficacy of serological markers for the early diagnosis of CD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This was a retrospective nested cohort study. Indirect immunofluorescence and enzyme-linked immunosorbent assay were used to detect ASCA IgG, ASCA IgA, AYMA IgG, AYCA IgG, FI2Y IgG, p-ANCA IgG, GAB IgG and PAB IgG in patient serum samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The positive rates of ASCA IgG, ASCA IgA, AYMA IgG, AYCA IgG, FI2Y IgG, p-ANCA IgG, GAB IgG and PAB IgG in patients with early CD, advanced CD and other intestinal diseases were 37.0% versus 56.8% versus 27.8%; 3.7% versus 20.5% versus 19.4%; 14.8% versus 2.3% versus 2.8%; 25.9% versus 9.1% versus 8.3%; 18.5% versus 15.9% versus 8.3%; 0.0% versus 2.8%, 18.5%; 13.6% versus 18.2% versus 16.7%; and 7.4% versus 20.5% versus 0.0%, respectively. The positive rates of ASCA IgG, AYCA IgG and PAB IgG were significantly different among the three groups (<i>p</i> &lt; 0.05). In 85.2% of early CD patients, at least one antibody was detected 1 year before diagnosis. The sensitivity of the ASCA/AYMA/AYCA/FI2Y/GAB combination for early diagnosis was 85.2%. The sensitivity of the ASCA/AYMA/AYCA/FI2Y/GAB/PAB/PANCA combination for differentiating CD from other diseases was 87.3%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>ASCA IgG and AYCA IgG have potential value in identifying the course of CD. AYCA IgG may be a potential marker for the early diagnosis of CD, and ASCA IgG indicates an advanced stage. The combination of ASCA, AYMA, AYCA, FI2Y, and GAB improves early diagnostic accuracy of CD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"181-189"},"PeriodicalIF":0.0,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.28","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138596565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Torsin-1A-interacting protein 1 as a predictive and immunological biomarker in cancer","authors":"Gurleen Kaur,&nbsp;Gurparsad Singh Suri,&nbsp;Dheeraj Shinde","doi":"10.1002/ila2.25","DOIUrl":"https://doi.org/10.1002/ila2.25","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Cancer poses a significant global challenge, and with the projected rise in cancer incidence, there is an urgent need to discover new targets and treatments to improve patient outcomes. Recent advancements in genomics technologies have enhanced our understanding of cancer's complexities and led to the emergence of pan-cancer analysis as a valuable approach for identifying tumor targets. Torsin-1A-interacting protein 1 (<i>TOR1AIP1</i>) is a membrane protein involved in various cellular processes. Emerging evidence suggests its potential involvement in cancer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this study, we conducted a comprehensive analysis of multiple databases to explore <i>TOR1AIP1</i> expression across different cancer types and stages. We also investigated its correlation with clinical outcomes, such as survival rates and drug sensitivity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The results of our analysis showed significant deregulation of <i>TOR1AIP1</i> expression in multiple cancer types and its association with clinical outcomes, with a particular emphasis on kidney renal clear cell carcinoma. The results of our study highlight the potential predictive value of <i>TOR1AIP1</i> in cancer prognosis and therapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study establishes a solid foundation and rationale for future experimental investigations, which will contribute to a deeper understanding of the significance of <i>TOR1AIP1</i> in different cancer types, specifically in kidney renal clear cell carcinoma.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"171-180"},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.25","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139042178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}