Comparison of the Performances of the Accunome DXcellence and Cepheid GeneXpert Assays in Detecting Influenza A and B Viruses and Respiratory Syncytial Virus in Nasopharyngeal Swab

iLABMED Pub Date : 2025-02-19 DOI:10.1002/ila2.74

Hui Hu, Qiankun Xuan, Tong Yu, Dongjiang Wang, Jian Guo, Wenjuan Wu

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Abstract

Background

Respiratory viruses, such as influenza A and B and respiratory syncytial virus (RSV), pose a severe threat to public health. The precise identification and distinction of these viruses are crucial in clinical laboratories. Here, we comparatively evaluated the performance of the Accunome DXcellence assay and the Cepheid GeneXpert assay in the detection of influenza A and B and RSV in nasopharyngeal swab specimens.

Methods

Four hundred archived nasopharyngeal swab specimens collected for routine clinical analysis were tested in parallel with the Accunome DXcellence assay and Cepheid GeneXpert assay. RNA standards were serially diluted and tested with the Accunome DXcellence assay to calculate the limit of detection (LOD).

Results

The positive and negative percentage agreement between the Accunome DXcellence assay and the Cepheid GeneXpert assay was as follows: 94.9% (74/78) and 100% (321/321), respectively, for influenza A; 98.1% (104/106) and 100% (293/293), respectively, for influenza B; and 100% (22/22) and 100% (377/377), respectively, for RSV. The LODs of the Accunome DXcellence assay for influenza A and B and RSV were 100, 87.5, and 62.5 copies/mL, respectively.

Conclusions

The performance of the Accunome DXcellence assay was similar to that of the Cepheid GeneXpert assay in the detection of influenza A, B, and RSV in nasopharyngeal swab specimens, indicating that the Accunome DXcellence assay is a useful diagnostic tool when these viruses are cocirculating.

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Accunome DXcellence与Cepheid GeneXpert检测鼻咽拭子甲型流感病毒、乙型流感病毒及呼吸道合胞病毒的比较

呼吸道病毒，如甲型流感和乙型流感以及呼吸道合胞病毒（RSV），对公众健康构成严重威胁。在临床实验室中，准确识别和区分这些病毒是至关重要的。本研究比较了Accunome DXcellence法和Cepheid GeneXpert法检测鼻咽拭子标本中甲型流感、乙型流感和RSV病毒的性能。方法采用Accunome DXcellence法和Cepheid GeneXpert法对临床常规分析的400份存档鼻咽拭子标本进行检测。依次稀释RNA标准品，用Accunome DXcellence法检测，计算检出限（LOD）。结果Accunome DXcellence法与Cepheid GeneXpert法检测甲型流感的正阴性符合率分别为94.9%（74/78）和100% (321/321)；乙型流感分别为98.1%（104/106）和100% (293/293)；RSV分别为100%（22/22）和100%（377/377）。Accunome DXcellence法检测甲型流感、乙型流感和RSV病毒的lod分别为100、87.5和62.5拷贝/mL。结论Accunome DXcellence检测方法与造父变星GeneXpert检测方法在鼻咽拭子标本中检测甲型流感、乙型流感和RSV的效果相似，表明Accunome DXcellence检测方法在这些病毒共传播时是一种有用的诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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