iLABMED最新文献

{"title":"Regulatory factors of ILC2 are therapeutic targets for lung inflammation","authors":"Lele Cui,&nbsp;Yajie Wang","doi":"10.1002/ila2.59","DOIUrl":"https://doi.org/10.1002/ila2.59","url":null,"abstract":"<p>Type 2 innate lymphoid cells (ILC2s) are an important class of innate immune cells that play a key role in regulating immune responses, maintaining tissue homeostasis, and participating in immune responses induced by inflammatory diseases. In lung inflammation, ILC2s drive the inflammatory response by secreting type 2 cytokines, and have a significant role in tissue repair and the maintenance of barrier function by secreting IL-9 and antimicrobial peptides. ILC2s activation and function are affected by various regulatory factors, including epithelial-derived alarmins such as IL-25, IL-33, and thymic stromal lymphopoietin, neurotransmitters, metabolites and hormones. These regulatory factors affect the development and activation of ILC2s through signaling pathways under different pathological conditions. An in-depth study of regulatory factors is expected to provide new targets and strategies for the treatment of lung inflammation.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"205-220"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.59","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiology, clinical features, and epidemiological analysis of diarrhea patients visiting a gastrointestinal clinic in a comprehensive hospital in Beijing, China, in 2023","authors":"Lihua Qi,&nbsp;Siwei Zhou,&nbsp;Dongmei Gu","doi":"10.1002/ila2.60","DOIUrl":"https://doi.org/10.1002/ila2.60","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To investigate the clinical features and epidemiology of diarrhea patients and analyze the current distribution of enteropathogens causing diarrhea in a comprehensive hospital in Beijing, China, in 2023.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>From April to October 2023, we enrolled patients with diarrheal diseases who visited the gastrointestinal <span>c</span>linic in our hospital. The patients' demographic, epidemiological, and clinical features were obtained via a questionnaire. Stool samples were examined for 20 enteropathogens by multiplex polymerase chain reaction testing.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We enrolled 260 patients; men and adults accounted for 55.77% and 95.77% of the patients, respectively. The median age was 37 years. Eighty-four enteropathogens, 72 bacteria and 12 viruses, were identified in 74 patients. Enteroaggregative <i>Escherichia coli</i> was the predominant agent. Patients with and without pathogens detected in stool samples showed no significant differences in age, sex, gastrointestinal symptoms, and stool characteristics. Possible food-related events were recorded in 57.31% of the patients. Leukocyte counts in patients with bacterial infections were higher than those of patients with viral infections and those with no detected pathogens (<i>p</i> &lt; 0.05). Seasonality of bacterial distribution was observed (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Bacteria were predominant pathogens among the diarrhea patients. The incidence of diarrhea was related to hot weather and foodborne illness. Bacterial diarrhea may cause systemic infection. The clinical symptoms of infectious diarrhea were usually non-specific and unrelated to the type of infection. Timely and comprehensive multi-pathogen surveillance might be helpful to detect suspected pathogens and promote epidemic prevention and control.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"197-204"},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.60","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective analysis of the relationship between dermatomyositis-associated interstitial lung disease and disease duration, age, arterial blood gas pH, and serum Cl− levels","authors":"Xu Zhang,&nbsp;Xuemei Wei,&nbsp;Xiaojuan Luan,&nbsp;Xiujuan Li,&nbsp;Jin Dong,&nbsp;Jingzhu Nan,&nbsp;Yanhong Gao","doi":"10.1002/ila2.56","DOIUrl":"https://doi.org/10.1002/ila2.56","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Dermatomyositis-associated interstitial lung disease (DM-ILD) represents a severe and insidious complication of dermatomyositis (DM). The study aimed to investigate the association between DM-ILD and arterial blood gas indices, serum ion levels, and the timing of interstitial lung disease onset, with the goal of identifying potential predictors for DM-ILD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The investigation involved the collection of basic data from 89 patients with DM hospitalized at the Chinese PLA General Hospital between January 2019 and April 2022, and 43 normal control patients hospitalized for physical examinations during the same period. Analyses were conducted to explore the relationship between DM-ILD, arterial blood gas indices, disease duration, and serum ions. A regression model to predict DM-ILD was developed using these indices, and a receiver operating characteristic curve was generated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences were observed in pH and PaO₂ between the control group and the disease group (<i>p</i> &lt; 0.05). The DM group exhibited higher levels of pH, actual bicarbonate, and base excess (BE) compared with the control group. In contrast, pH and BE levels were lower in the DM-ILD group than in the DM group, with these differences being statistically significant (<i>p</i> &lt; 0.05). Interstitial lung disease was correlated with the duration of the disease and pH levels (<i>p</i> &lt; 0.05). The cutoff values for age, disease duration, pH, and Cl⁻ were 55.5 years, 5.5 years, 7.432, and 101.5 mmol/L, respectively. The model demonstrated a prediction sensitivity and specificity for DM-ILD of 0.809 and 0.722, respectively, with an area under the curve of 0.809.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Arterial blood gas analysis and serum Cl⁻ levels may assist in predicting DM-ILD. A combined monitoring approach involving arterial blood gas pH, disease duration, age, and serum Cl⁻ levels could enhance the accuracy of DM-ILD predictions and hold significant clinical evaluation potential.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"168-177"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.56","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgo biloba active compounds can modulate the development of acute mountain sickness and ischemic stroke as discovered by network pharmacology and molecular docking","authors":"Haoran Guo,&nbsp;Xueran Kang,&nbsp;Ying Xu,&nbsp;Chengbin Wang,&nbsp;Chi Wang","doi":"10.1002/ila2.58","DOIUrl":"https://doi.org/10.1002/ila2.58","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>A combination of molecular docking, molecular dynamics simulations, and herbal network pharmacology was used to investigate the shared key targets and potential mechanisms underlying the preventive effects of <i>Ginkgo biloba</i> active compounds against acute mountain sickness (AMS) and ischemic stroke (IS).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was used to screen the main active compounds of <i>Ginkgo biloba</i> and their corresponding targets. We obtained AMS-related genes by mining several databases and cross-correlated them with key active compounds of <i>Ginkgo biloba</i> to identify relevant action targets for treating AMS. The STRING database was used to construct a protein–protein interaction network of the effect of <i>Ginkgo biloba</i> active compounds on AMS targets. The expression of genes in the network was analyzed in an IS dataset to identify common key targets of <i>Ginkgo biloba</i> active compounds for both AMS and IS prevention.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The intersection between the targets of <i>Ginkgo biloba</i> active compounds and AMS-related genes identified 43 overlapping genes. Analysis of the protein–protein interaction network showed that <i>VEGFA</i>, <i>TP53</i>, <i>SERPINE1</i>, and <i>PTGS2</i> were among the key hub genes. Analysis of the IS dataset identified significant differences in the expression levels of <i>CAT</i>, <i>TP53</i>, <i>CXCL8</i>, <i>NFKBIA</i>, and <i>PTGS2</i>. These genes were used to construct a visual nomogram prediction model for IS prognosis with promising clinical implications. Molecular docking and molecular dynamics simulations indicated that sesamin stably targeted and bound to PTGS2.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Active ingredients of <i>Ginkgo biloba</i>, including luteolin, quercetin, and sesamin, have the potential to modulate the development of AMS and IS through targeted interactions with key proteins, including TP53, CXCL8, NFKBIA, PTGS2, and CAT.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"178-196"},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.58","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy and computed tomography diagnostic value of bedaquiline-containing regimens in the treatment of drug-resistant pulmonary tuberculosis","authors":"Saiduo Liu,&nbsp;Xinchun Ye,&nbsp;Fang Cheng,&nbsp;Kaijia Wu,&nbsp;Jiandan Yu,&nbsp;Hongye Ning,&nbsp;Jichan Shi,&nbsp;Hongzhou Lu,&nbsp;Wei Chen","doi":"10.1002/ila2.57","DOIUrl":"https://doi.org/10.1002/ila2.57","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study investigated the clinical efficacy of bedaquiline-containing regimens in the treatment of drug-resistant pulmonary tuberculosis and the diagnostic value of computed tomography (CT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We retrospectively analyzed the clinical diagnosis, treatment, and CT imaging data of patients with drug-resistant pulmonary tuberculosis treated in Wenzhou Central Hospital from 1 January to 31 December 2022. According to whether the treatment regimen contained bedaquiline, the patients were divided into an observation group (bedaquiline tablets + background regimen) and a control group (background regimen). The clinical efficacy and pulmonary CT changes before and after treatment were analyzed in both groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After 24 weeks of treatment, there was no statistically significant difference in the white blood cell count or concentrations of hemoglobin, alanine aminotransferase, serum albumin, or creatinine between the two groups (<i>t</i> = 0.71, 0.93, 0.05, 0.18, and 0.08, respectively; <i>p</i> &gt; 0.05). After 4, 8, and 12 weeks of treatment, there was no statistically significant difference in the sputum culture-negative conversion rate between the two groups (<i>χ</i>² = 2.67, 0.48, and 1.82, respectively; <i>p</i> &gt; 0.05). At 24 weeks of treatment, the sputum culture-negative conversion rate in the observation group reached 100%, which was significantly higher than that in the control group (<i>χ</i>² = 3.97, <i>p</i> &lt; 0.05). The effective absorption rates on chest imaging in the two groups of patients at 12 weeks were 83.33% and 57.89%, respectively. At 24 weeks of treatment, the effective absorption rates were 88.00% and 65.85% in the two groups, with a statistically significant difference (<i>χ</i>² = 3.98; <i>p</i> &lt; 0.05). There were significant differences in cavity absorption at 24 weeks (<i>χ</i>² = 4.33, <i>p</i> &lt; 0.05) and 48 weeks after treatment (<i>χ</i>² = 10.63, <i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The addition of bedaquiline to the background regimen improved the sputum culture-negative conversion rate and chest imaging effective rate. Patients achieved good results at the end of the 24-week treatment period.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"149-156"},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.57","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis","authors":"Ying Li, Ruiqi Xiao, Peicen Zou, Yue Du, Qinglin Lu, Jun Cui, Yajuan Wang","doi":"10.1002/ila2.55","DOIUrl":"https://doi.org/10.1002/ila2.55","url":null,"abstract":"Early identification of pathogenic bacteria and monitoring residual status are essential for accurate treatment of neonatal bacterial meningitis (NBM).Clinical data and specimens were collected from neonates with NBM. Bacterial cultures and RT‐PCR of blood and cerebrospinal fluid (CSF) were compared to assess the positivity rate, sensitivity and specificity of each method.RT‐PCR had a higher positivity rate compared with cultures, regardless of whether antibiotics had been used prior to specimen collection. After 1 week of regular antibiotic treatment, the number of pathogen DNA copy numbers in CSF was either undetectable or significantly reduced compared with previous levels.RT‐PCR is expected to provide a basis for the precise application of antibiotics and the course of treatment for NBM, particularly in patients with negative cultures or those who have already been treated with antibiotics.","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"58 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141929178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid development of neurological infection in a patient with human immunodeficiency virus following schistosomiasis: A case report","authors":"Yan Liu, Guoqiang Zhou, Wei Jiang, Xianglong Kong","doi":"10.1002/ila2.54","DOIUrl":"https://doi.org/10.1002/ila2.54","url":null,"abstract":"HIV‐1 and schistosomal infections present significant global health challenges, and neurological manifestations of these pathogens are easily misdiagnosed due to their rarity. Here, we report the case of a 36‐year‐old patient with acquired immunodeficiency syndrome who was initially diagnosed with human immunodeficiency virus‐1 (HIV‐1) infection 4 years earlier, although untreated for approximately 3 years until he began antiretroviral therapy (ART) following a tuberculosis diagnosis and hospitalization. Despite achieving virological suppression of HIV‐1 1 year after ART, he was readmitted with high fever and headache. Initial therapy for suspected tuberculosis based on clinical performance and brain imaging features failed, and further investigation confirmed an intracranial infection caused by schistosomiasis. Following anti‐schistosomal treatment and optimized ART, the patient recovered fully and was discharged. This case of a patient in Asia infected with human immunodeficiency virus (HIV) who rapidly developed a neurological infection subsequent to acquiring schistosomiasis highlights the need for awareness of such coinfections in patients with HIV.","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"50 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141814788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “The exploration of cell population data in clinical use: Beyond infectious diseases”","authors":"","doi":"10.1002/ila2.53","DOIUrl":"10.1002/ila2.53","url":null,"abstract":"<p>Huang S, Liu Y, Qian L, Zhou J, Wang D. The exploration of cell population data in clinical use: beyond infectious diseases. iLABMED. 2024;2(2):125–40.</p><p>In the last paragraph of the “3.2.2 COVID-19” section, the text “(mean lymphocyte volume [LV] x LV − SD)/(mean lymphocyte conductivity [LC])” was incorrect. This should have read: “(mean lymphocyte volume [LV] × LV − SD)/(mean lymphocyte conductivity [LC])”.</p><p>In paragraph 5 of the “3.2.3 Other infectious diseases” section and Table 1, the texts “95% <i>Cl</i>” was incorrect. These should have read: “95% <i>CI</i>”.</p><p>We apologize for these errors.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"226"},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.53","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141831344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity","authors":"Zijin Zhao, Yanbo You, Shaowei Hua, Xinxin Shen, Lingjun Li, Xuejun Ma","doi":"10.1002/ila2.51","DOIUrl":"https://doi.org/10.1002/ila2.51","url":null,"abstract":"Fluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time PCR (qPCR) assays, but they require a complex probe design. To eliminate the addition of fluorescent probes for RAA, an EvaGreen dye‐based recombinase‐aided amplification (EvaGreen‐RAA) assay using self‐avoiding molecular recognition system (SAMRS) primers was developed.The SAMRS primers effectively avoided the production of primer dimers, thus improving the detection sensitivity, while EvaGreen dye was used to quantitatively measure the amplified products in real time. Using Staphylococcus aureus (SA) and Listeria monocytogenes (LM) as examples, EvaGreen‐RAA with SAMRS primers was developed. As a reference and comparison, a traditional fluorescence probe RAA method and a RAA with SAMRS primers (SAMRS‐RAA) for detecting SA and LM were also investigated. Serial dilutions of recombinant plasmids were used to evaluate the sensitivity of the assays. Unenriched and enriched simulated milk samples were used to evaluate the limits of detection (LOD) of these methods. Using high‐resolution melting (HRM) was used to explore the sensitivity of the dual EvaGreen‐RAA assay.The sensitivity of the fluorescent RAA method for detecting SA and LM was 10 copies/μL using plasmids and the sensitivity of the SAMRS‐RAA and EvaGreen‐RAA for detecting SA and LM plasmids was 1 copies/μL. The LOD values of the EvaGreen‐RAA for SA and LM in unenriched simulated milk samples were 100 and 50 CFU/mL, respectively, and the LOD value for both SA and LM using enriched simulated milk samples was 10 CFU/mL. EvaGreen‐RAA had linear amplification in real time in the range of 1–105 copies/μL of the plasmids of SA and LM. The sensitivity of the dual EvaGreen‐RAA assay for SA and LM was estimated to be 102 CFU/mL.A real‐time quantitative EvaGreen‐RAA method for detecting SA and LM was developed, which eliminates the need to design complex RAA probes. This dye‐based RAA with SARMS primers provides a new strategy for simplifying fluorescence probe RAA and allowing the detection of multiple pathogens, which has many potential applications.","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"55 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141650395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A predictive model for ischemic colitis: Integrating clinical and laboratory parameters","authors":"Chonghui Hu,&nbsp;Xiuying Zhao,&nbsp;Bo Jiang,&nbsp;Xuan Jiang,&nbsp;Yutang Ren,&nbsp;Jiaojiao Guo","doi":"10.1002/ila2.52","DOIUrl":"10.1002/ila2.52","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>We aimed to develop a predictive model for the clinical diagnosis of ischemic colitis (IC).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Clinical data were collected from patients with acute IC lesions who were diagnosed and admitted to Beijing Tsinghua Changgung Hospital from January 2016 to December 2022. These patients were included in the IC case group in this retrospective observational study. The control group comprised patients aged ≥40 years who were diagnosed with abdominal pain during the same period, excluding those with IC. All patients were divided into a training and test sets based on the time window. Least absolute shrinkage and selection operator regression was used to screen risk factors for the occurrence of IC. Logistic stepwise regression (maximum likelihood ratio method) was performed in multifactorial analysis, and a diagnostic prediction model for IC was established using R language. The area under the receiver operating characteristic (ROC) curve (AUC) was examined to assess differentiation using working ROC curves. We used bootstrap resampling (1000 times) for internal validation. Model calibration curves and decision curve analysis (DCA) were also applied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our study indicates that constipation, hematochezia, neutrophil counts, and specific abdominal computed tomography (CT) (plain scan) findings, including intestinal wall edema and thickening, intestinal lumen stenosis, and dilation, are independent predictors of IC. The predictive model exhibited high discriminative ability with an AUC of 0.9788 in the training set, and the calibration and DCA curves demonstrated excellent model performance. After validation, the AUC remained robust at 0.9868, underscoring the model's reliability in predicting IC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>According to our model, constipation accompanied by hematochezia necessitates careful consideration of IC. Abdominal CT (plain scan) is an effective diagnostic tool for IC, and it is common for patients to exhibit elevated neutrophil counts. The predictive model, demonstrating high discriminative ability and accuracy, shows promise for practical application in clinical settings, aiding in the early diagnosis and management of IC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 3","pages":"157-167"},"PeriodicalIF":0.0,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.52","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141649614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}