iLABMED最新文献

{"title":"A preliminary study of the mechanism of compound hearing loss caused by ototoxic drugs combined with impulse noise","authors":"Xin Qiu,&nbsp;Qing-qing Jiang,&nbsp;Wei-wei Guo,&nbsp;Ning Yu,&nbsp;Shi-ming Yang","doi":"10.1002/ila2.34","DOIUrl":"10.1002/ila2.34","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hearing loss (HL) is becoming increasingly common and is more commonly caused by noise, ototoxic substances, or a combination of ototoxic factors. However, so far, few studies have examined the mechanism by which compound factors cause HL. The only relevant study is about occupational ototoxic substances combined with environmental noise at 85–110 dB SPL. In this study, to address the shortcomings of existing research, we innovatively focused on HL induced by loud noise (impulse noise, &gt;160 dB SPL) combined with common ototoxic drugs. The aim of this study was to establish and validate a mature animal model, and then to compare the characteristics of audiology, pathomorphology and molecular features, and to preliminarily predict pathogenesis in compound HL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>We selected guinea pigs to construct in vivo HL model groups for different extents of exposure, including a blank control group, a single-drug group, a single-impulse noise group, and a compound group. The animal model of the mature compound HL group was established using gentamicin combined with impulse noise. We then performed audiological and pathological verification. We analyzed the auditory brainstem response (ABR), pathological morphology of the cochlea, and molecules (including important self-radicals, cytokines, and apoptosis signal transduction pathway proteins in the pathogenesis of drug- and noise-induced HL), compared the effect of different extents of exposure on HL, and preliminarily predict the pathogenic mechanism of compound HL.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Four groups of animal models were established successfully and verified by audiology and pathology. Regarding audiology, there were no significant differences in the ABR thresholds before exposure (<i>p</i> &gt; 0.05), but differences emerged among the groups after exposure. Notably, after 3, 7, and 14 days of exposure, there were significant differences in the ABR thresholds between the compound group and both the drug and noise groups (<i>p</i> &lt; 0.01), and after 14 days, the HL of the compound group was much more severe (greater than the linear sum of single-factor HL group). Regarding the pathomorphology, compared with the control group, the cochleae were damaged to different degrees in the factor exposure groups. The drug group had the least severe HL, the noise group had serious HL (<i>p</i> &lt; 0.05), and the compound group had the most severe HL (<i>p</i> &lt; 0.01). The compound group's damage was greater than the linear sum of the single-factor group in many ways, such as the loss and damage o","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"38-52"},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140245830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful treatment of severe monkeypox case with advanced HIV infection using plasma from smallpox-vaccinated healthy population: A case report","authors":"Chuming Chen,&nbsp;Weiming Yao,&nbsp;Siran Huang,&nbsp;Ling Peng,&nbsp;Shiyan Feng,&nbsp;Weibo Wu,&nbsp;Yunlan Yi,&nbsp;Zhiqiang Cheng,&nbsp;Yang Yang,&nbsp;Yan Yang,&nbsp;Liuqing Yang,&nbsp;Hongzhou Lu,&nbsp;Fuxiang Wang","doi":"10.1002/ila2.32","DOIUrl":"10.1002/ila2.32","url":null,"abstract":"<p>Since the first reported case of monkeypox in the UK in May 2022, there has been an upward trend in monkeypox cases and a global outbreak. However, reports of severe cases are relatively limited. In this study, we report a case of severe monkeypox in a patient with HIV. The patient presented with skin lesions that started on his face and around the penis and persisted for several months. Throughout the course of the disease, he received systematic symptomatic supportive treatment, topical remedies, and special care for the rash. He also underwent cidofovir antiviral therapy and smallpox-vaccinated healthy population-derived plasma therapy in succession, with the condition ultimately showing improvement after plasma treatment. After more than 3 months of hospitalization, he fully recovered. To the best of our knowledge, this is the first reported use of smallpox-vaccinated healthy population-derived plasma in the treatment of severe monkeypox cases.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"60-66"},"PeriodicalIF":0.0,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.32","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140255168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic approaches for monkeypox virus","authors":"Guohao Fan,&nbsp;Jiahua Kuang,&nbsp;Shengjie Zhang,&nbsp;Yang Yang,&nbsp;Yingxia Liu,&nbsp;Hongzhou Lu","doi":"10.1002/ila2.36","DOIUrl":"10.1002/ila2.36","url":null,"abstract":"<p>Mpox (formerly Monkeypox) is a zoonotic infection caused by Monkeypox virus (MPXV). Since 2022, Mpox epidemics have occurred in many non-endemic countries and regions, leading the World Health Organization to declare a public health emergency of international concern. With the persistent transmission and evolution of MPXV, symptoms of Mpox have become milder, with some infections being asymptomatic. In addition, MPXV has become more contagious. Therefore, rapid and accurate diagnosis and screening of MPXV is vital to prevent and control MPXV epidemics. Here, we review and summarize the technical details, application scenarios, and the advantages and disadvantages of MPXV-specific diagnostic methods.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"6-13"},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140259101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From bench to bedside: Opportunities and challenges for iLABMED","authors":"Tetsuya Asakawa,&nbsp;Yi-Wei Tang,&nbsp;Hongzhou Lu","doi":"10.1002/ila2.33","DOIUrl":"https://doi.org/10.1002/ila2.33","url":null,"abstract":"<p>Since the new <i>iLABMED</i> was founded in June 2023, it has published three issues with 21 articles (till Dec 2023). As a journal majored in laboratory medicine, <i>iLABMED</i> also has to face many opportunities and challenges of laboratory medicine. This editorial summarized the main opportunities and challenges faced by <i>iLABMED</i>. The future prospects of laboratory medicine, which must be highlighted by <i>iLABMED</i> were also discussed based on a brief review of the current advances in laboratory medicine. <i>iLABMED</i> will continue to provide a useful platform for general-interested, insightful, and informative articles with high quality.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140291380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa infections and improper storage conditions influence the performance of 1,3-β-d-glucan in diagnosis of invasive fungal infections","authors":"Zilan Wei,&nbsp;Jie Xu,&nbsp;Fang Yuan,&nbsp;Wendong Fang,&nbsp;Jiahui Wu,&nbsp;Youliang Wang,&nbsp;Shuiping Chen","doi":"10.1002/ila2.35","DOIUrl":"https://doi.org/10.1002/ila2.35","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The association between 1,3-<i>β</i>-<span>d</span>-glucan (BDG) levels and infections caused by <i>Pseudomonas aeruginosa</i> or <i>Streptococcus pneumoniae</i>, and the stability of BDG under different storage conditions are unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Strains of <i>Pseudomonas aeruginosa</i> and <i>S</i>. <i>pneumoniae</i> were grown in medium and human serum. The BDG concentrations in culture supernatants were measured. The specificity and stability of BDG were also evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>P</i>. <i>aeruginosa</i> produced high levels of BDG in Luria–Bertani medium (&gt;4 × 10⁴ pg/mL) and human serum (527.0 pg/mL), whereas <i>S</i>. <i>pneumoniae</i> produced low levels of BDG in THY medium (175.6 pg/mL) and human serum (78.3 pg/mL). The BDG produced by these two bacteria was specifically degraded by 1,3-<i>β</i>-<span>d</span>-glucanase. BDG was degraded when stored at different temperatures, decreasing by 22.5% and 9.3% at −20°C and −70°C, respectively, for 63 days; by 30.7% at 4°C for 12 days; and by 12.6% and 22.0% at 37°C for 6 and 12 h.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>BDG false-positivity must be considered in patients with bacteremia caused by <i>P</i>. <i>aeruginosa</i> when diagnosing invasive fungal infection. Human serum samples for the BDG test in medical facilities should be tested as soon as possible or stored at low temperatures before testing.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"53-59"},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140291358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers for predicting efficacy of chimeric antigen receptor T cell therapy and their detection methods","authors":"Bo Zou,&nbsp;Yanzhou Song,&nbsp;Ning Li,&nbsp;Zhongyi Fan,&nbsp;Jie Li,&nbsp;Yuanzheng Peng,&nbsp;Wanshan Wei,&nbsp;Yuzi Zhang,&nbsp;Yinan Su,&nbsp;Xianmin Meng,&nbsp;Hongzhou Lu,&nbsp;Xingding Zhang,&nbsp;Xiaohua Tan,&nbsp;Qibin Liao","doi":"10.1002/ila2.31","DOIUrl":"10.1002/ila2.31","url":null,"abstract":"<p>Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment, alongside surgery, radiotherapy, and chemotherapy. After several decades of development, chimeric antigen receptor T (CAR-T) cell therapy, a promising branch of adoptive T-cell therapy, has demonstrated superior efficacy and safety in comparison to other cell therapies in the treatment of cancer. At present, CAR-T cells are predominantly used to treat hematological malignancies, although their application in solid tumors is being readily investigated. Although numerous studies have examined the biomarkers associated with the safety of CAR-T cell therapy, few have evaluated predictors of CAR-T cell therapeutic efficacy. Thus, the primary objective of this review article was to provide a comprehensive overview of the factors predicting the efficacy of CAR-T cell therapy, with a particular focus on biomarkers and their detection methods.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"14-26"},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of new diagnostic targets for hepatitis B virus-induced liver fibrosis","authors":"Ying Wang,&nbsp;Shuo Qin,&nbsp;Meng Yang,&nbsp;Xiaoling Wang","doi":"10.1002/ila2.30","DOIUrl":"10.1002/ila2.30","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Liver fibrosis is a transitional stage from hepatitis to cirrhosis, and hepatitis B virus (HBV) is the most common cause of liver disease. Transcriptome sequencing technology and bioinformatics analysis are increasingly being used to screen diagnostic targets for liver fibrosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The GSE171294 dataset of HBV-induced liver fibrosis tissue and normal tissue was obtained from the Gene Expression Omnibus (GEO) public database and used to screen for differentially expressed mRNAs using R software. mRNAs with |log fold change| &gt;1 and <i>p</i> &lt; 0.05 were considered to be differentially expressed. A heat map was drawn to visualize the expression patterns of the differentially expressed mRNAs. To screen for candidate target mRNAs, the differentially expressed mRNAs were annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis. Finally, a protein–protein interaction (PPI) network was constructed to analyze the relationships between the differentially expressed mRNAs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 243 differentially expressed mRNAs were identified (<i>p</i> &lt; 0.05); 129 were up-regulated and 114 were down-regulated. The up-regulated and down-regulated mRNAs were significantly enriched in 16 and 8 KEGG pathways, respectively. The enriched KEGG pathways included Salmonella infection, Protein processing in the endoplasmic reticulum, IL-17 signaling pathway, and Aldosterone synthesis and secretion. The enriched GO terms were related mainly to cell proliferation, apoptosis, endoplasmic reticulum complex assembly, and myosin synthesis. The PPI network contained 161 nodes and 120 pairs of interactions. The top 10 key nodes were <i>CAV1, CD4, NR3C1, PDIA3, EZR, IRF4, SOX9, HSP90AB1, CD40,</i> and <i>SEC13</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Bioinformatics analysis of the transcriptome sequencing data in the GSE171294 dataset identified <i>CD4</i>, <i>NR3C1</i>, and <i>EZR</i> and other genes at key nodes as new targets for the treatment of liver fibrosis caused by HBV. These results provide new insights for HBV-induced liver fibrosis research and clinical treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 1","pages":"27-37"},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.30","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139453544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the epidemic characteristics of Mycoplasma pneumoniae infection around COVID pandemic","authors":"Hongmei Sun,&nbsp;Li Xiao","doi":"10.1002/ila2.27","DOIUrl":"https://doi.org/10.1002/ila2.27","url":null,"abstract":"<p><i>Mycoplasma pneumoniae</i> (<i>M. pneumoniae</i>) is a cell wall-less respiratory pathogen causing community-acquired pneumonia and extrapulmonary manifestations. It is transmitted through close contact and shows periodic regional outbreaks. The coronavirus disease (COVID-19) pandemic interfered with the global spread of <i>M. pneumoniae</i>. A large-scale post-COVID outbreak is currently ongoing in China. To help physicians better understand and manage this epidemic, we provide this review summarizing current knowledge on the pathogenesis, epidemic characteristics, macrolide resistance, diagnostic methods, and clinical treatment strategies for this pathogen.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"148-157"},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.27","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The important role of skin biopsies in the diagnosis of mpox","authors":"Wun-Ju Shieh","doi":"10.1002/ila2.29","DOIUrl":"10.1002/ila2.29","url":null,"abstract":"<p>Skin biopsy is an important, efficient, and safe procedure for diagnosing infectious dermatopathy, including mpox. An optimal approach of laboratory diagnosis of mpox should include obtaining a skin punch biopsy from pertinent lesions for pathologic evaluation in addition to conventional swab or scrap for molecular testing.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"143-147"},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138586130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutating Escherichia coli caused multiple organ dysfunction syndrome","authors":"Lihua Qi,&nbsp;Wencheng Xue,&nbsp;Xuelin Liu,&nbsp;Shaofu Qiu,&nbsp;Jie Liu","doi":"10.1002/ila2.26","DOIUrl":"10.1002/ila2.26","url":null,"abstract":"<p>Pathogenic <i>Escherichia coli</i> is of great concern in the clinical setting. But few reports have demonstrated the variation in disease course. We present a severe case of multiple organ dysfunction syndrome caused by <i>E. coli</i> infection. Pathogens isolated from blood and urine samples harboured many virulence factors. Whole-genome sequencing and conventional analyses showed that the isolates experienced beneficial variations, both genetically and phenotypically, during the disease course. These findings showed that <i>E. coli</i> can cause systemic symptoms and informed us of the importance of assessing the reasons for such variations in pathogens occurring in vivo.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"1 3","pages":"190-195"},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138585754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}