Diagnostic value of exosomal N-glycan profiles for microvascular invasion in hepatocellular carcinoma

iLABMED Pub Date : 2024-10-14 DOI:10.1002/ila2.61
Huijuan Feng, Junping Ke, Liping Zheng, Chenjun Huang, Lijuan Liu
{"title":"Diagnostic value of exosomal N-glycan profiles for microvascular invasion in hepatocellular carcinoma","authors":"Huijuan Feng,&nbsp;Junping Ke,&nbsp;Liping Zheng,&nbsp;Chenjun Huang,&nbsp;Lijuan Liu","doi":"10.1002/ila2.61","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Microvascular invasion (MVI) was a critical high-risk factor for postoperative recurrence and adverse prognosis in patients with hepatocellular carcinoma (HCC), and there were no reliable non-invasive pre-operative diagnostic markers. The exosomal N-glycan profile was closely related to the invasion and immune escape of HCC. Therefore, this article investigated the expression of N-glycan profiles in serum exosomes of patients with HCC and its clinical significance for MVI.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Serum samples from 210 patients with HCC were collected and randomly divided into modeling and validation cohorts. The abundances of N-glycans in serum exosomes with different MVI grades were determined. A diagnostic model for MVI in HCC based on N-glycosylation was constructed and the diagnostic value was analyzed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In the modeling cohort, comparing groups M0 with M2, the area under the receiver operating characteristic (AUC) of the diagnostic model namely SUM (AUC<sub>SUM</sub>) was 0.861, the sensitivity of SUM was 92.68%, and the specificity of SUM was 79.41%, all of which were higher than the seven individual indexes (containing Peak 1, Peak 6, Peak 9, Peak 10, Peak 12, AFP, and PIVKA-II). In the comparison between the M1 and M2 groups, the AUC<sub>SUM</sub> was 0.749, the sensitivity of SUM was 79.07%, and the specificity of SUM was 76.60%, all of which were higher than the seven individual indexes. When comparing the M0 and M1 groups, the AUC<sub>SUM</sub> was 0.712, the sensitivity of SUM was 88.57%, and the specificity of SUM was 65.00%. The AUC<sub>SUM</sub> and sensitivity of SUM were higher than the seven individual indexes and the specificity of SUM was slightly lower than that of AFP (68.18%) but higher than other individual indexes. The results of the validation cohort were similar to those of the modeling cohort.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The SUM model of serum exosomes can serve as an auxiliary diagnostic index for MVI staging in patients with HCC.</p>\n </section>\n </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"2 4","pages":"238-247"},"PeriodicalIF":0.0000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.61","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"iLABMED","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ila2.61","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background

Microvascular invasion (MVI) was a critical high-risk factor for postoperative recurrence and adverse prognosis in patients with hepatocellular carcinoma (HCC), and there were no reliable non-invasive pre-operative diagnostic markers. The exosomal N-glycan profile was closely related to the invasion and immune escape of HCC. Therefore, this article investigated the expression of N-glycan profiles in serum exosomes of patients with HCC and its clinical significance for MVI.

Methods

Serum samples from 210 patients with HCC were collected and randomly divided into modeling and validation cohorts. The abundances of N-glycans in serum exosomes with different MVI grades were determined. A diagnostic model for MVI in HCC based on N-glycosylation was constructed and the diagnostic value was analyzed.

Results

In the modeling cohort, comparing groups M0 with M2, the area under the receiver operating characteristic (AUC) of the diagnostic model namely SUM (AUCSUM) was 0.861, the sensitivity of SUM was 92.68%, and the specificity of SUM was 79.41%, all of which were higher than the seven individual indexes (containing Peak 1, Peak 6, Peak 9, Peak 10, Peak 12, AFP, and PIVKA-II). In the comparison between the M1 and M2 groups, the AUCSUM was 0.749, the sensitivity of SUM was 79.07%, and the specificity of SUM was 76.60%, all of which were higher than the seven individual indexes. When comparing the M0 and M1 groups, the AUCSUM was 0.712, the sensitivity of SUM was 88.57%, and the specificity of SUM was 65.00%. The AUCSUM and sensitivity of SUM were higher than the seven individual indexes and the specificity of SUM was slightly lower than that of AFP (68.18%) but higher than other individual indexes. The results of the validation cohort were similar to those of the modeling cohort.

Conclusion

The SUM model of serum exosomes can serve as an auxiliary diagnostic index for MVI staging in patients with HCC.

Abstract Image

求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信