iLABMED最新文献

{"title":"iLABMED: A Translational Medical Journal Embracing a New Era","authors":"Tetsuya Asakawa, Yi-Wei Tang, Hongzhou Lu","doi":"10.1002/ila2.70051","DOIUrl":"https://doi.org/10.1002/ila2.70051","url":null,"abstract":"<p>The third anniversary of <i>iLABMED</i> will be commemorated in June 2026. The editorial leadership extends sincere appreciation to all authors, reviewers, editors, and readers for their continued support and contributions. Over the past year, the journal has made steady progress across multiple dimensions, reflecting its growing academic influence and sustained commitment to translational medicine.</p><p>In 2025, <i>iLABMED</i> published 48 peer-reviewed articles encompassing a wide range of experimental and clinical research topics. The journal is currently indexed in several major bibliographic databases, including the Chinese Science Citation Database (CSCD), the Directory of Open Access Journals (DOAJ), and Scopus. Applications for inclusion in the Emerging Sources Citation Index (ESCI) and PubMed Central (PMC) are under active review. Collectively, these developments represent important milestones toward enhancing the journal's global visibility and scientific impact, and they provide a strong foundation for the continued advancement of <i>iLABMED</i> in 2026.</p><p>Over the past year, <i>iLABMED</i> published a diverse and scientifically rigorous body of work encompassing clinically relevant and methodologically robust studies. Kobialka et al. conducted a systematic comparison of ten commercially available extraction protocols for point-of-need molecular diagnostics, highlighting the critical roles of flexibility, reliability, and sample-specific performance in the selection of diagnostic workflows [<span>1</span>]. Ren et al. provided a comprehensive review of recent advances in molecular point-of-care testing systems, identifying intelligent, shared, and cloud-based platforms as key directions for future development [<span>2</span>].</p><p>Several contributions focused on oncology and immunology. Zhao et al. identified CD48 as a breast cancer–associated gene linked to immune cell infiltration and clinical prognosis, supporting its potential utility as a predictive biomarker [<span>3</span>]. Cai et al. reviewed recent progress in flow cytometry, emphasizing ongoing improvements in analytical accuracy and clinical applicability as major trends shaping the field [<span>4</span>]. Wei et al. demonstrated that synaptophysin expression is associated with tumor differentiation and prognosis in gastric cancer, providing clinically meaningful prognostic insights [<span>5</span>].</p><p>In the areas of infectious diseases and clinical microbiology, Gao et al. analyzed antimicrobial susceptibility patterns of <i>Eggerthella lenta</i> isolates, revealing inconsistencies with conventional therapeutic approaches and underscoring the need for updated clinical treatment guidelines [<span>6</span>]. Tang et al. critically reviewed precision diagnostic strategies for <i>Helicobacter pylori</i> infection and antimicrobial resistance, offering clinically actionable guidance to inform diagnostic and therapeutic decision-making [<span>7</span>]. Dong et a","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147614847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA Vaccination for Treating Allergy by Modulating Allergen-Specific Immunity","authors":"Kun Guo,&nbsp;Lijing Liu,&nbsp;Mingyun Shen,&nbsp;Karin Loré,&nbsp;Ang Lin","doi":"10.1002/ila2.70047","DOIUrl":"https://doi.org/10.1002/ila2.70047","url":null,"abstract":"<p>This <i>News and Views</i> article discusses the rationale for anti-allergy vaccine development using a lipid nanoparticle-mRNA platform. The authors provide additional evidence that supports the potential of using the mRNA approach to combat allergic diseases.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"56-58"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147614902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA Vaccination for Treating Allergy by Modulating Allergen-Specific Immunity","authors":"Kun Guo,&nbsp;Lijing Liu,&nbsp;Mingyun Shen,&nbsp;Karin Loré,&nbsp;Ang Lin","doi":"10.1002/ila2.70047","DOIUrl":"https://doi.org/10.1002/ila2.70047","url":null,"abstract":"<p>This <i>News and Views</i> article discusses the rationale for anti-allergy vaccine development using a lipid nanoparticle-mRNA platform. The authors provide additional evidence that supports the potential of using the mRNA approach to combat allergic diseases.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"56-58"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147614903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Analysis of Blood Donor Deferral Reasons and Optimization Suggestions for Deferral Policies","authors":"Limei Feng,&nbsp;Li Liu,&nbsp;Rui Wang,&nbsp;Ting Zhang,&nbsp;Wei Guo,&nbsp;Yang Yu","doi":"10.1002/ila2.70050","DOIUrl":"10.1002/ila2.70050","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Blood donor deferral is a critical safeguard to protect both donor health and transfusion safety. However, deferral practices can adversely affect blood availability and donor retention. The aim of this study is to evaluate deferral patterns among voluntary blood donors and to identify opportunities to optimize deferral strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Deferral data were retrospectively collected from the blood transfusion management system of a single center between January 2019 and December 2023. Statistical analyses were performed using SPSS version 25, with <i>p</i> &lt; 0.001 considered statistically significant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 148,282 blood donors were included, of whom 18,343 (12.37%) were deferred. Temporary deferrals accounted for 10.95% (16,240/148,282) and permanent deferrals for 1.42% (2103/148,282). The leading causes of temporary deferral were abnormal alanine aminotransferase (ALT) levels, representing 51.33% (9415/18,343) of all deferrals, followed by low hemoglobin levels at 12.20% (2237/18,343), lipemic blood at 7.12% (1306/18,343), non-medical reasons including time constraints, procedural complexity, and psychological concerns at 6.90% (1265/18,343), hypertension at 4.08% (749/18,343), and medication use at 2.26% (414/18,343). Permanent deferrals were most frequently attributed to hepatitis B surface antigen (HBsAg) positivity at 4.12% (755/18,343) and syphilis antibody positivity at 2.89% (530/18,343), while nucleic acid reactivity accounted for 0.26% (48/18,343).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Targeted donor education, optimization of pre-donation screening, and refinement of deferral criteria may improve donor recruitment and retention while maintaining transfusion safety.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"37-46"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147585122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"iLABMED: A Translational Medical Journal Embracing a New Era","authors":"Tetsuya Asakawa,&nbsp;Yi-Wei Tang,&nbsp;Hongzhou Lu","doi":"10.1002/ila2.70051","DOIUrl":"https://doi.org/10.1002/ila2.70051","url":null,"abstract":"&lt;p&gt;The third anniversary of &lt;i&gt;iLABMED&lt;/i&gt; will be commemorated in June 2026. The editorial leadership extends sincere appreciation to all authors, reviewers, editors, and readers for their continued support and contributions. Over the past year, the journal has made steady progress across multiple dimensions, reflecting its growing academic influence and sustained commitment to translational medicine.&lt;/p&gt;&lt;p&gt;In 2025, &lt;i&gt;iLABMED&lt;/i&gt; published 48 peer-reviewed articles encompassing a wide range of experimental and clinical research topics. The journal is currently indexed in several major bibliographic databases, including the Chinese Science Citation Database (CSCD), the Directory of Open Access Journals (DOAJ), and Scopus. Applications for inclusion in the Emerging Sources Citation Index (ESCI) and PubMed Central (PMC) are under active review. Collectively, these developments represent important milestones toward enhancing the journal's global visibility and scientific impact, and they provide a strong foundation for the continued advancement of &lt;i&gt;iLABMED&lt;/i&gt; in 2026.&lt;/p&gt;&lt;p&gt;Over the past year, &lt;i&gt;iLABMED&lt;/i&gt; published a diverse and scientifically rigorous body of work encompassing clinically relevant and methodologically robust studies. Kobialka et al. conducted a systematic comparison of ten commercially available extraction protocols for point-of-need molecular diagnostics, highlighting the critical roles of flexibility, reliability, and sample-specific performance in the selection of diagnostic workflows [&lt;span&gt;1&lt;/span&gt;]. Ren et al. provided a comprehensive review of recent advances in molecular point-of-care testing systems, identifying intelligent, shared, and cloud-based platforms as key directions for future development [&lt;span&gt;2&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Several contributions focused on oncology and immunology. Zhao et al. identified CD48 as a breast cancer–associated gene linked to immune cell infiltration and clinical prognosis, supporting its potential utility as a predictive biomarker [&lt;span&gt;3&lt;/span&gt;]. Cai et al. reviewed recent progress in flow cytometry, emphasizing ongoing improvements in analytical accuracy and clinical applicability as major trends shaping the field [&lt;span&gt;4&lt;/span&gt;]. Wei et al. demonstrated that synaptophysin expression is associated with tumor differentiation and prognosis in gastric cancer, providing clinically meaningful prognostic insights [&lt;span&gt;5&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;In the areas of infectious diseases and clinical microbiology, Gao et al. analyzed antimicrobial susceptibility patterns of &lt;i&gt;Eggerthella lenta&lt;/i&gt; isolates, revealing inconsistencies with conventional therapeutic approaches and underscoring the need for updated clinical treatment guidelines [&lt;span&gt;6&lt;/span&gt;]. Tang et al. critically reviewed precision diagnostic strategies for &lt;i&gt;Helicobacter pylori&lt;/i&gt; infection and antimicrobial resistance, offering clinically actionable guidance to inform diagnostic and therapeutic decision-making [&lt;span&gt;7&lt;/span&gt;]. Dong et a","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147614898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Relevance and Diagnostic Utility of Standard Serum Biomarkers in Advanced Chronic Kidney Disease: A Single-Center Observational Study From Bangladesh","authors":"Fazlul Haque,&nbsp;Md. Samiul Islam,&nbsp;Nafsoon Rahman,&nbsp;Ayub Zawad,&nbsp;Sadia Afrin,&nbsp;Pulak Kanti Palit,&nbsp;Tanjil Hasan,&nbsp;Md. Isahak Ali,&nbsp;Shafin Ahmed,&nbsp;Akram Hossain,&nbsp;Marina Khatun,&nbsp;Tabassuma Marzia Prome,&nbsp;Md. Shah Alam,&nbsp;Ashekul Islam","doi":"10.1002/ila2.70040","DOIUrl":"10.1002/ila2.70040","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Chronic kidney disease (CKD) is a growing public health concern in Bangladesh, with an increasing prevalence and limited access to early diagnostic tools. This study aimed to evaluate conventional and emerging biochemical markers in an integrated manner for assessing CKD progression in Bangladeshi adults.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A primarily cross-sectional with exploratory repeated measurement study was conducted at Chittagong Medical College Hospital between May and October 2024. We included 95 participants (70 patients with CKD and 25 healthy controls). Clinical and sociodemographic data were collected. Blood samples were collected to measure serum concentrations of creatinine, uric acid, parathyroid hormone (PTH), vitamin D, phosphate, albumin, and glutamate-pyruvate transaminase and the estimated glomerular filtration rate (eGFR). Data were processed using Prism 10. Normality was assessed using the Shapiro–Wilk test. The <i>t</i>-test and Kruskal–Wallis test were used to compare variables.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Patients with CKD showed significantly higher serum creatinine, uric acid, and PTH concentrations, lower serum vitamin D and albumin concentrations, and a lower eGFR than controls (all <i>p</i> &lt; 0.05). In patients with CKD, elevated PTH and phosphate concentrations combined with reduced calcium and vitamin D concentrations indicate mineral bone disorder, whereas hypoalbuminemia reflects poor nutritional status and underlying systemic inflammation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study highlights the diagnostic utility of serum uric acid, PTH, albumin, and vitamin D concentrations in conjunction with traditional renal markers for efficient detection and monitoring of CKD progression in Bangladesh. Regular screening and effective management of comorbidities, such as hypertension and diabetes, are essential to reduce CKD-related morbidity and mortality.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"27-36"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147585155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presentation and Management of Addison's Disease in a 10-Year-Old Boy: A Case Report","authors":"Srinivasan Sivannan,&nbsp;Mohammed Azhar Hussain,&nbsp;Syed Mohammed Hussaini,&nbsp;Pasumarthi Tejashree,&nbsp;Karthikeyan Elumalai","doi":"10.1002/ila2.70053","DOIUrl":"https://doi.org/10.1002/ila2.70053","url":null,"abstract":"<p>Addison's disease, or primary adrenal insufficiency, is a rare endocrine disorder characterized by impaired adrenal hormone production. We report a case of a 10-year-old boy with congenital adrenal hyperplasia and secondary Addison's disease on long-term corticosteroid therapy who presented with recurrent non-bilious vomiting, low-grade fever, headache, and epigastric pain. Physical examination revealed short stature (below the third percentile) and overweight status (90th percentile), likely related to prolonged steroid use. He was managed with intravenous fluids, adjustment of antibiotics, and optimized steroid therapy, leading to significant clinical improvement. This case highlights the complexity of managing pediatric adrenal insufficiency, particularly in patients receiving chronic corticosteroids. Lifelong individualized hormone replacement, careful dose adjustment, and regular monitoring are essential to prevent adrenal crises and long-term metabolic complications. A multidisciplinary approach involving endocrinologists, pediatricians, and supportive care teams is crucial for optimal outcomes in children with Addison's disease.</p>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"59-63"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147614845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Machine Learning Model Based on Blood Indices for the Differential Diagnosis of Colorectal Cancer and Colorectal Polyps","authors":"Wenhui Yan,&nbsp;Lin Zhu,&nbsp;Hongming Wei,&nbsp;Jie Feng,&nbsp;Yanhong Gao","doi":"10.1002/ila2.70049","DOIUrl":"10.1002/ila2.70049","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>With high colorectal cancer (CRC) incidence, accurate early differentiation of precancerous polyps is critical for prognosis; while the gold-standard colonoscopy-biopsy is limited by invasiveness, cost and poor scalability, and routine blood tests lack efficiency with simple indicator combinations, machine learning's feature-mining capacity offers a solution. This study aimed to develop and evaluate a machine learning system for differentiating patients with CRC and colorectal polyps using routine blood indices.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective analysis was conducted on the clinical data of 284 patients with CRC and 79 patients with colorectal polyps who were diagnosed at the Chinese PLA General Hospital from October 2021 to February 2024. The extreme gradient boosting (XGBoost) algorithm was used to establish a machine learning model using demographic characteristics and routine blood indices. The Shapley additive explanation method was used to evaluate feature importance.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The constructed XGBoost model achieved high levels in differentiating CRC and colorectal polyps, with a precision of 0.906, a recall of 0.817, an accuracy of 0.791, and an area under the receiver operating characteristic curve of 0.869. The Shapley additive explanation showed that the top five important features were fibrinogen, carcinoembryonic antigen, plasma thrombin time, ferritin, and D-dimer.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The XGBoost machine learning model based on blood indices has certain application value in the differential diagnosis of CRC and colorectal polyps, providing a new efficient tool for auxiliary diagnosis to assist clinical decision-making.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"47-55"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance Evaluation of Triggering Patient-Based Real-Time Quality Control Alarms for Insufficient Remaining Amounts of K+, Na+, and Cl− Reagents Based on Three Data Truncation Methods","authors":"Rong Zheng,&nbsp;Xuemei Wei,&nbsp;Jie Feng,&nbsp;Lin Zhu,&nbsp;Fei Guo,&nbsp;Hui Ding,&nbsp;Yanhong Gao","doi":"10.1002/ila2.70021","DOIUrl":"10.1002/ila2.70021","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;In clinical laboratories, reagent depletion can significantly compromise the accuracy of K&lt;sup&gt;+&lt;/sup&gt;, Na&lt;sup&gt;+&lt;/sup&gt;, and Cl&lt;sup&gt;−&lt;/sup&gt; measurements, posing risks to patient safety. Patient-based real-time quality control (PBRTQC) has emerged as a valuable tool for early detection of analytical errors. However, the effectiveness of PBRTQC may be influenced using data processing methods. Among these, truncation, tail-retention, and tail-shrinkage are three commonly used data truncation techniques. Despite their potential, the clinical early-warning performance of PBRTQC with these methods under reagent depletion conditions remains unclear. Therefore, this study aims to evaluate the clinical early-warning efficacy of PBRTQC when integrated with these three data truncation methods (truncation, tail-retention, and tail-shrinkage) for K&lt;sup&gt;+&lt;/sup&gt;, Na&lt;sup&gt;+&lt;/sup&gt;, and Cl&lt;sup&gt;−&lt;/sup&gt; measurements during reagent depletion scenarios.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A controlled experimental design was used with routine patient test results as the control group and simulated reagent depletion scenarios as experimental groups. PBRTQC was applied to integrated datasets processed by the three truncation methods. Alarm timeliness rates for detecting abnormal results were evaluated for K&lt;sup&gt;+&lt;/sup&gt;, Na&lt;sup&gt;+&lt;/sup&gt;, and Cl&lt;sup&gt;−&lt;/sup&gt;.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Under internal standard solution depletion, the number of samples required for error detection (NPed) for K&lt;sup&gt;+&lt;/sup&gt; using the truncation, tail-retention, and tail-shrinkage methods was 38, 10, and 10, respectively. For Na&lt;sup&gt;+&lt;/sup&gt;, the NPed was 55, 5, and 10, respectively, while for Cl&lt;sup&gt;−&lt;/sup&gt;, it was 0, 11 and 16, respectively. During diluent depletion, K&lt;sup&gt;+&lt;/sup&gt; and Na&lt;sup&gt;+&lt;/sup&gt; showed 0 with the truncation method but 10 for both the tail-retention and tail-shrinkage methods; Cl&lt;sup&gt;−&lt;/sup&gt; showed 0 with the truncation method but 11 with the tail-retention method and 16 with the tail-shrinkage method. With reference solution depletion, the NPed for K&lt;sup&gt;+&lt;/sup&gt; using the truncation, tail-retention, tail-shrinkage methods was 42, 35, and 39, respectively; the NPed for Na&lt;sup&gt;+&lt;/sup&gt; was 135, 25, and 25, respectively; and the NPed for Cl&lt;sup&gt;−&lt;/sup&gt; was 140, 31, and 36, respectively.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;During a shortage of reagents, the effectiveness of early warning of the PBRTQC program was the highest with the tail-retention method, followed by the tail-shrinkage method. The tru","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"18-26"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147585163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-Intrinsic Forkhead Box A2 Regulates the Lymph Node Metastasis of Papillary Thyroid Carcinoma by Regulating T-Cell Apoptosis","authors":"Ping Yang,&nbsp;Xueying Zhang,&nbsp;Wen Deng,&nbsp;Fangning Gao,&nbsp;Shujia Peng,&nbsp;Guoqiang Bao,&nbsp;Lijuan Yuan","doi":"10.1002/ila2.70039","DOIUrl":"10.1002/ila2.70039","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Lymph node metastasis is a significant indicator of recurrence and mortality in thyroid carcinoma and often guides treatment decisions. However, the characteristics and specific mechanisms of various cancer cells that metastasize to lymph nodes are not clear. This study aims to characterize the cellular heterogeneity and transcriptomic dynamics of thyroid cancer. We further seek to elucidate the role of the key transcription factor FOXA2 in orchestrating a pro-metastatic and immunosuppressive program, specifically its regulation of T-cell activity and apoptosis. Our ultimate goal is to identify potential therapeutic strategies for thyroid cancer with lymph node metastasis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Using single-cell RNA-seq analyses, we investigated the dynamic of cellular heterogeneity transcriptome regulation and microenvironmental factors in 15,591 single cells from paired samples of primary and lymph node-metastasized tumors. The function of key factor was further investigated using data from public database and in vitro experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We found that among all the six subclusters identified, the cancer cells (cluster 3) with higher tumor different ability (tumor different score) evolved into metastatic clusters infiltrating into lymph nodes. We also showed that the immune response-activating cell surface receptor signaling pathway is enriched in malignant cells of the metastatic lymph node. The interactions between T cells and other cells were weakened, and CCLs-mediated signals out/income T-cells are more likely to affect the interactions. Tumor-intrinsic forkhead box A2 (FOXA2) orchestrated the thyroid cancer cell immunologic/metastatic signature and its expression is related to prognosis. FOXA2 knockdown promoted thyroid cancer cell migration, proliferation, and T-cell apoptosis. Moreover, its expression significantly influenced T-cell activity by the CCL2/LCN2-mediated signal pathway.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings reveal that FOXA2 regulates cancer cell immunity by influencing T-cell apoptosis in thyroid cancer. Our results provide a potential pharmacological strategy for immune therapy in thyroid cancer with lymph node metastasis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100656,"journal":{"name":"iLABMED","volume":"4 1","pages":"5-17"},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ila2.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}