Frontiers in Laboratory Medicine最新文献_第5页

A simple assay for direct colorimetric detection of prostatic acid phosphatase (PAP) at fg levels using biphosphonated loaded PEGylated gold nanoparticles 一种使用双磷酸负载聚乙二醇化金纳米颗粒直接比色法检测前列腺酸性磷酸酶(PAP)在fg水平的简单方法

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/j.flm.2017.11.005

Maroua Ben Haddada , Fatima Aouidat , Maelle Monteil , Marc Lecouvey , Marc Lamy de la Chapelle , Jolanda Spadavecchia

{"title":"A simple assay for direct colorimetric detection of prostatic acid phosphatase (PAP) at fg levels using biphosphonated loaded PEGylated gold nanoparticles","authors":"Maroua Ben Haddada , Fatima Aouidat , Maelle Monteil , Marc Lecouvey , Marc Lamy de la Chapelle , Jolanda Spadavecchia","doi":"10.1016/j.flm.2017.11.005","DOIUrl":"https://doi.org/10.1016/j.flm.2017.11.005","url":null,"abstract":"<div><p>A fast and sensitive colorimetric assay for the determination of prostatic acid phosphatase (PAP) levels using biphosphonates-PEG-coated gold nanoparticles (BP-PEG-AuNPs) has been developed. Addition of different amounts of PAP to the AuNPs results in a significant change of color, from a red-wine color to purple, and finally to blue. The PAP should bind onto the AuNPs surface and crosslink the AuNPs inducing their aggregation and as a consequence a color change of the solution from wine red to purple/blue. The color change was monitored by UV–vis spectrophotometry or with the naked eye. The ensuing concentration-dependent red-shift of surface plasmon resonance absorption allows the determination concentration within the fg range in 10 min. Importantly, the visual detection limit (1 ng/mL) allows the rapid differential diagnosis with naked eye or image analyzer. This demonstrates that the color modification observed with BP-PEG-AuNP is effectively due to an interaction between PAP and BP.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 4","pages":"Pages 186-191"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.11.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91725524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Highly crystalline sphere and rod-shaped TiO2 nanoparticles: A facile route to bio-polymer grafting 高结晶球形和棒状TiO2纳米粒子:生物聚合物接枝的便捷途径

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/j.flm.2017.12.003

Céline Falentin-Daudré, Jean-Sebastien Baumann, Véronique Migonney, Jolanda Spadavecchia

{"title":"Highly crystalline sphere and rod-shaped TiO2 nanoparticles: A facile route to bio-polymer grafting","authors":"Céline Falentin-Daudré, Jean-Sebastien Baumann, Véronique Migonney, Jolanda Spadavecchia","doi":"10.1016/j.flm.2017.12.003","DOIUrl":"https://doi.org/10.1016/j.flm.2017.12.003","url":null,"abstract":"<div><p>The goal of this report is to provide a versatile one step method based on UV irradiation in order to graft a bioactive polymer bearing sulfonate groups called poly(sodium styrene sulfonate) (polyNaSS) onto titanium nanoparticles (TiO<sub>2</sub> NPs) through covalent bond. The synthetic approach consists in two steps: (1) fabrication of TiO<sub>2</sub> nanocrystals with different shape and size by solvothermal method previous hydrolysis of titanium(IV)isopropoxide (TTIP) in the presence of benzyl alcohol (BzOH) and acetic acid (AcOH) at 180 °C, (2) TiO<sub>2</sub> NPs were immersed in a solution of monomer bearing sulfonate group and were placed under UV irradiation to induce the formation of radical to initiate the polymerization of the monomer. To demonstrate the success of the procedure, modified nano-surfaces were characterized by different techniques including colorimetric method by complexation, ATR–FTIR and SEM (EDS). This approach is promising to develop bio-active polymer–TiO<sub>2</sub> nanoparticles as carriers and further successful application in the field of regenerative-nanomedicine.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 4","pages":"Pages 217-223"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91725525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Opportunities and challenges for lung cancer screening in China 中国肺癌筛查的机遇与挑战

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/j.flm.2017.11.003

Chuanli Ren, Chongxu Han

引用次数: 0

Guide for Authors 作者指南

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/S2542-3649(18)30016-5

引用次数: 0

Highly crystalline sphere and rod-shaped TiO2 nanoparticles: A facile route to bio-polymer grafting 高结晶球形和棒状TiO2纳米粒子:生物聚合物接枝的便捷途径

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI: 10.1016/J.FLM.2017.12.003

C. Falentin-Daudré, Jean-Sébastien Baumann, V. Migonney, J. Spadavecchia

引用次数: 10

Evaluation of EGF, EGFR, and E-cadherin as potential biomarkers for gastrointestinal cancers EGF、EGFR和E-钙粘蛋白作为胃肠道癌症潜在生物标志物的评估

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.08.001

Liejun Jiang , Steven Hochwald , Shuang Deng , Yanhui Zhu , Chunyan Tan , Qiulian Zhong , Ying Zhou , Hongying Zhao , Huayi Huang

{"title":"Evaluation of EGF, EGFR, and E-cadherin as potential biomarkers for gastrointestinal cancers","authors":"Liejun Jiang , Steven Hochwald , Shuang Deng , Yanhui Zhu , Chunyan Tan , Qiulian Zhong , Ying Zhou , Hongying Zhao , Huayi Huang","doi":"10.1016/j.flm.2017.08.001","DOIUrl":"https://doi.org/10.1016/j.flm.2017.08.001","url":null,"abstract":"<div><p>Biomarkers for early diagnosis and prognostic evaluation of cancers are still unsatisfactory in clinical management. The seeking of new biomarkers with better performance is still a challenge. EGF, EGFR, and E-cad are soluble biomarkers with clinical potential. ELISA was used to analyze serum EGF, EGFR, and E-cad from 58 gastrointestinal cancer patients and 20 control subjects. Immunohistochemistry was used to analyze Ki-67 expression in tumors. The results were correlated with clinical features. This study found that the preoperative and postoperative serum EGF and EGFR levels were significantly higher than in controls. The preoperative EGF and E-cad levels were significantly higher than postoperative levels. There was no correlation between EGF, EGFR, and E-cad levels and serum CEA or tumor Ki-67 scores. The serum EGF level was significantly higher in high TNM stage, with lymph node involvement patients than in low TNM stage, no lymph node involvement patients. Serum EGF and EGFR are potential biomarkers for the diagnosis and screening of gastrointestinal cancers. Inter-combination analysis of three biomarkers has improved the performance of the assays.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 3","pages":"Pages 135-140"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72272157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Study on CD4+T lymphocytes of new Uygur HIV infectors in Urumqi area 乌鲁木齐地区维吾尔族HIV新感染者CD4+T淋巴细胞的研究

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.10.001

Guohong Huang, Yanjie Cui, Zhiwei Li, Rui Huang, Changmin Wang

{"title":"Study on CD4+T lymphocytes of new Uygur HIV infectors in Urumqi area","authors":"Guohong Huang, Yanjie Cui, Zhiwei Li, Rui Huang, Changmin Wang","doi":"10.1016/j.flm.2017.10.001","DOIUrl":"https://doi.org/10.1016/j.flm.2017.10.001","url":null,"abstract":"<div><p><em>Objective:</em> To understand the immune status of new Uygur HIV infectors in Urumqi area by detecting and analyzing CD4<sup>+</sup>T lymphocytes. <em>Methods:</em> Flow cytometry was used to detect absolute counts of CD4<sup>+</sup>T lymphocytes in 92 whole blood samples and the Student’s <em>t</em> test was used for analysis. <em>Results:</em> The mean absolute counts of CD4<sup>+</sup>T lymphocytes of 92 new Uygur HIV infectors was (305.9 ± 168.3) cells/μL. No statistical significances were found in different genders and different transmission routes (<em>P</em> > 0.05). A negative correlation was showed between absolute counts of CD4<sup>+</sup>T lymphocytes and HIV viral load. <em>Conclusions:</em> Two third of the new Uygur HIV infectors in Urumqi area have entered the AIDS phase, so monitoring and antiviral therapy were needed timely. Local government must strengthen publicity and enlarge the monitoring range of CD4<sup>+</sup>T lymphocytes. Early diagnosis and intervention could improve the survival quality of HIV infectors.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 3","pages":"Pages 122-124"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72272584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can gut flora changes be new biomarkers for depression? 肠道菌群变化能成为抑郁症的新生物标志物吗？

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.08.002

Ping Lin , Qingtian Li

引用次数: 1

High-sensitivity cardiac troponins I sandwich assay by immunomagnetic microparticle and quantum dots 免疫磁微粒和量子点夹心法测定心肌肌钙蛋白I的高灵敏度

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.09.001

Hua Jiang, Jiapin Zhu, Wenwei Liu, Fengsheng Cao

引用次数: 5

First case of Kerstersia gyiorum isolated from a patient with chronic osteomyelitis in China 中国首例从慢性骨髓炎患者中分离到的颧Kerstersia

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.08.003

Y. Lan, Qun Yan, Yuying Yan, Wenen Liu

引用次数: 3