Frontiers in Laboratory Medicine最新文献_第6页

The application of Student’s t-test in internal quality control of clinical laboratory 学生t检验在临床实验室内部质量控制中的应用

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.09.002

Yangchun Feng, Yan-Chun Huang, Xiu-Min Ma

引用次数: 33

Green extraction of endemic plants to synthesize gold nanoparticles for theranostic applications 地方植物的绿色提取合成用于治疗应用的金纳米颗粒

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.10.003

Anne-Laure Morel , Sophie Giraud , Anne Bialecki , Hanane Moustaoui , Marc Lamy de La Chapelle , Jolanda Spadavecchia

{"title":"Green extraction of endemic plants to synthesize gold nanoparticles for theranostic applications","authors":"Anne-Laure Morel , Sophie Giraud , Anne Bialecki , Hanane Moustaoui , Marc Lamy de La Chapelle , Jolanda Spadavecchia","doi":"10.1016/j.flm.2017.10.003","DOIUrl":"https://doi.org/10.1016/j.flm.2017.10.003","url":null,"abstract":"<div><p>We report a simple and original method to synthesize water-soluble gold nanoparticles in which a polyphenolic fraction, extracted by two plants originated from Reunion Island, was mixed to tetrachloroauric acid (HAuCl<sub>4</sub>) leading to shell-like hybrid flavonoid-metal nanoparticles (NPs). The nanoparticles have been characterized by ultra-violet/visible Raman spectroscopy, and also by electron microscopy imaging (TEM). The results of these analytical green methodologies highlight nanometric sized, stable, hybrid complexes of about 15 nm, or flower-shaped 40 nm diameter with outer surface rich of functional chemical groups. This paper, through an original chemical approach, will occupy an important position in the field of Nanomedicine, and the authors hope that novel perspectives will be proposed for the development of a double nanotheranostic (plasmonic phototherapeutic and X-ray based computed tomography (CT scan)) in order to treat cancer simultaneously by plasmonic phototherapy (PTT), and at the same time to allow the visualization by X-ray based computed tomography.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 3","pages":"Pages 158-171"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.10.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72272154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

The application of Student’s t-test in internal quality control of clinical laboratory Student t检验在临床实验室内部质量控制中的应用

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.09.002

Feng Yang-chun , Huang Yan-chun , Ma Xiu-min

{"title":"The application of Student’s t-test in internal quality control of clinical laboratory","authors":"Feng Yang-chun , Huang Yan-chun , Ma Xiu-min","doi":"10.1016/j.flm.2017.09.002","DOIUrl":"https://doi.org/10.1016/j.flm.2017.09.002","url":null,"abstract":"<div><h3>Background</h3><p>James O. Westgard advised that the relation between internal quality control (IQC) and statistical methods, such as <em>t</em>-test, should be seriously considered. The results of IQC fully fit in the requirements of the <em>t</em>-test. But there was no research on analyzing the results of IQC by <em>t</em>-test. So the study aimed to explore the value of <em>t</em>-test for IQC by analyzing a specific example.</p></div><div><h3>Methods</h3><p>The results of IQC about luteotropichormone (LH) were collected in July 2015 in two instruments from a clinical laboratory as a specific example. The pre-determined target value of IQC was evaluated by a one-sample <em>t</em>-test first. Then the consistency of two instruments was analyzed by comparing their results of IQC with two paired samples <em>t</em>-test.</p></div><div><h3>Results</h3><p>In middle LH level of No. 1 instrument, though there was no out-of-control IQC result, the difference between the mean of IQC results in July 2015 and the pre-determined target value was statistically significant (<em>t</em>  =0.027). So it was earlier to find whether the pre-determined target value was inappropriate by using a one-sample <em>t</em>-test when no out-of-control results appeared in the traditional IQC chart. For high LH level, the difference between the IQC results of two instruments was statistically significant (<em>t</em>  =0.020) in July 2015, so it was helpful to discover the inconsistency of two instruments by analyzing their IQC results with two paired samples <em>t</em>-test.</p></div><div><h3>Conclusions</h3><p>The one-sample <em>t</em>-test was helpful to guide the correction of pre-determined target value of IQC. Two paired samples <em>t</em>-test was beneficial to improve consistency between two instruments.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 3","pages":"Pages 125-128"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72272582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Evaluation of EGF, EGFR, and E-cadherin as potential biomarkers for gastrointestinal cancers EGF、EGFR和e -钙粘蛋白作为胃肠道癌症潜在生物标志物的评估

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.08.001

Liejun Jiang, S. Hochwald, S. Deng, Yan-bo Zhu, Tan Chunyan, Qiulian Zhong, Z. Ying, Hongying Zhao, Huayi Huang

引用次数: 2

High-sensitivity cardiac troponins I sandwich assay by immunomagnetic microparticle and quantum dots 免疫磁性微粒子和量子点夹心法测定高灵敏度心肌肌钙蛋白I

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.09.001

Hua Jiang , Jiapin Zhu , Wenwei Liu , Fengsheng Cao

{"title":"High-sensitivity cardiac troponins I sandwich assay by immunomagnetic microparticle and quantum dots","authors":"Hua Jiang , Jiapin Zhu , Wenwei Liu , Fengsheng Cao","doi":"10.1016/j.flm.2017.09.001","DOIUrl":"https://doi.org/10.1016/j.flm.2017.09.001","url":null,"abstract":"<div><h3>Objective</h3><p>High-sensitivity cardiac troponins I (hs-cTnI) has been endorsed as a standard biomarker for diagnosis of acute myocardial infarction (AMI) and can provide information for risk stratification. Our study aimed to develop a quantum-dot-labeled magnetic immunoassay for the hs-cTnI detection.</p></div><div><h3>Methods</h3><p>We developed a novel immunomagnetic microparticle and quantum dots-based sandwich assay (QM-cTnI) that employs digital recording of fluorescence. Immunomagnetic microparticle conjugated with two monoantibodies was used as capture antibody, and two biotinylated monoantibodies were used as detection antibody, then quantum dots conjugated streptavidin served as a fluorescence bioprobe and the signal was recorded by the luminescence spectrometer.</p></div><div><h3>Results</h3><p>Hs-cTnI was quantifiable in serum within one hour in the QM-cTnI assay which has a detection limit of 0.047 ng/mL and the effective measuring range was 0–40 ng/mL. The intra-assay and interassay coefficients of variation were 8.56% and 8.92% at 0.2 ng/mL, 5.83% and 7.66% at 4 ng/mL, and 6.21% and 8.76% at 20 ng/mL, respectively. All of the recovery rates were within the range of 98.11–101.89% and the average recovery rate was 99.69%. This assay performed well compared with FDA-approved SIEMENS ADVIA Centaur XP immunoassay system by 104 patient serum samples (R<sup>2</sup> = 0.9951). High-concentration interferents (bilirubin, triglycerides, and hemoglobin) did not influence the test results indicating good specificity of the MQ-cTnI assay.</p></div><div><h3>Conclusion</h3><p>Our results demonstrated that hs-cTnI sandwich assay with the immunomagnetic microparticle and quantum dots-based multi-mAb approach is a sensitive, accurate, and quantitative method for the detection of disease biomarkers.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 3","pages":"Pages 107-113"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72272585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Can gut flora changes be new biomarkers for depression 肠道菌群的变化能成为抑郁症的新生物标志物吗

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.08.002

Ping Lin, Qingtian Li

引用次数: 1

First case of Kerstersia gyiorum isolated from a patient with chronic osteomyelitis in China 中国首例从一名慢性骨髓炎患者身上分离出的脊状Kerstersia

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.08.003

You Lan, Qun Yan, Yuying Yan, Wenen Liu

引用次数: 3

Release of cardiac troponin from healthy and damaged myocardium 健康和受损心肌肌钙蛋白的释放

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/j.flm.2017.09.003

Alan H.B. Wu

{"title":"Release of cardiac troponin from healthy and damaged myocardium","authors":"Alan H.B. Wu","doi":"10.1016/j.flm.2017.09.003","DOIUrl":"https://doi.org/10.1016/j.flm.2017.09.003","url":null,"abstract":"<div><p>Cardiac troponins T and I are proteins released into serum after cardiac injury, and are the standard biomarkers for patients presenting to the emergency department with a suspicion of acute myocardial infarction (AMI). Cardiac troponin that appears in blood within a few hours is due to release from the cytosolic pool. A sustained irreversible release over the ensuing days is due to the degradation of the myofibrils, although recent data have challenged this concept. The analytical sensitivity for troponin assays have significantly improved since the initial release of commercial troponin assays over 20 years ago. As a result, the specificity of troponin for AMI has steadily declined, with abnormal concentrations seen in many non-cardiac diseases such as renal failure, sepsis, pulmonary embolism, and cardiac injury after chemotherapy such as with trastuzumab and doxorubicin. There are many theories as to how troponin is released into blood from patients with reversible myocardial ischemia and from patients with cardiac damage that is not related to ischemia. These theories include release of free subunit release through bleb formation, transient imbalance of oxygen supply and demand such as what occurs with acute vasospasm of coronary vessels, pulmonary embolism with right heart damage, apoptosis, acute cardiac stress leading to release of catecholamines and integrins, myocardial stretching, inflammation, and release of degraded troponin peptides. The mechanisms for these etiologies are reviewed.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"1 3","pages":"Pages 144-150"},"PeriodicalIF":0.0,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2017.09.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72272156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Study on CD4+T lymphocytes of new Uygur HIV infectors in Urumqi area 乌鲁木齐地区维吾尔族HIV新发感染者CD4+T淋巴细胞的研究

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.10.001

Guohong Huang, Yanjie Cui, Zhiwei Li, Rui-Jie Huang, Changmin Wang

引用次数: 0

Association between apolipoprotein C3 gene promoter polymorphisms and fasting triglyceride level: A meta-analysis 载脂蛋白C3基因启动子多态性与空腹甘油三酯水平之间的关系:一项荟萃分析

Frontiers in Laboratory Medicine Pub Date : 2017-09-01 DOI: 10.1016/J.FLM.2017.10.002

Shi Cheng, B. Pang, C. Gu, Shipeng Sun, Cheng An, Zhen-peng Zhang, Jun Li, Guijian Liu

引用次数: 1