Frontiers in Laboratory Medicine最新文献

Association of ATG16L1 genetic variant with Helicobacter pylori infection in Egyptian patients with chronic gastritis disease ATG16L1基因变异与埃及慢性胃炎患者幽门螺杆菌感染的关系

Frontiers in Laboratory Medicine Pub Date : 2018-12-01 DOI: 10.1016/j.flm.2019.08.001

Aya Hamdy Abd-EL Aziz , Heba Mohamed Abdallah , Samah Mohamed Awad , Osama EL-Bahr , Ashraf Khalil , Gamal Youssef Abo Riaa

{"title":"Association of ATG16L1 genetic variant with Helicobacter pylori infection in Egyptian patients with chronic gastritis disease","authors":"Aya Hamdy Abd-EL Aziz , Heba Mohamed Abdallah , Samah Mohamed Awad , Osama EL-Bahr , Ashraf Khalil , Gamal Youssef Abo Riaa","doi":"10.1016/j.flm.2019.08.001","DOIUrl":"10.1016/j.flm.2019.08.001","url":null,"abstract":"<div><h3>Background</h3><p>Helicobacter pylori infection remains a major risk for gastroduodenal inflammation and ulceration with capability to disrupt the defensive autophagy pathway in the gastric mucosal cell thus producing resilient infection. A single-nucleotide mutation in the autophagy gene <em>ATG16L1</em> which controls the host immune responses to viruses and bacteria was identified as a risk factor for increasing survival and persistence of many microbial infections.</p></div><div><h3>Aim</h3><p>To assess the association of <em>ATG16L1</em> T300A gene mutation with the susceptibility to H. pylori infection in patients with chronic gastritis disease.</p></div><div><h3>Methods</h3><p><em>ATG16L1</em> T300A mutation was determined by TaqMan genotyping allele specific discrimination real-time PCR assay applying specific primers, (<em>rs2241880</em> A/G) and probes for A and G allele.</p></div><div><h3>Result</h3><p>Patients with mutant homozygous GG genotype was associated with a 4.5 times increase in the risk of H. pylori infection relative to the wild homozygous AA and the heterozygous AG genotypes (p value = 0.009, OR = 4.472; 95% CI, 0.07–0.74). The carriage rate of the mutant G allele was significantly higher in H. pylori positive cases (64%) relative to (42%) in H. pylori negative cases (p value = 0.002, OR = 2.455, 95% CI, 0.3 (0.09–1.01), and this was associated with about a 2.5 times increase in the risk for H. pylori infection.</p></div><div><h3>Conclusion</h3><p>Genetic variant <em>ATG16L1</em> T300A mutation increased the risk of H. pylori infection in Egyptian patients with chronic gastritis disease.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 4","pages":"Pages 134-140"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2019.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76118781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA profiling of cerebrospinal fluid from patients with intracerebral haemorrhage 脑出血患者脑脊液MicroRNA谱分析

Frontiers in Laboratory Medicine Pub Date : 2018-12-01 DOI: 10.1016/j.flm.2019.07.001

Qinghai Shi , Di Ge , Qi Yang , Li Wang , Jianfeng Fu

{"title":"MicroRNA profiling of cerebrospinal fluid from patients with intracerebral haemorrhage","authors":"Qinghai Shi , Di Ge , Qi Yang , Li Wang , Jianfeng Fu","doi":"10.1016/j.flm.2019.07.001","DOIUrl":"10.1016/j.flm.2019.07.001","url":null,"abstract":"<div><h3>Objective</h3><p>Differential expression profiles of free microRNAs (miRNAs) in cerebrospinal fluid (CSF) from patients with intracerebral haemorrhage (ICH) and controls were analysed to identify miRNA markers associated with ICH-induced brain injury.</p></div><div><h3>Methods</h3><p>Five patients with acute ICH were enrolled alongside five control inpatients without CNS diseases. CSF specimens were collected during initial lumbar puncture after admission, total free RNAs were extracted from CSF, and miRNAs were detected by Agilent Human miRNA V21.0 chip experiments. Differential expression profiles were statistically analysed.</p></div><div><h3>Results</h3><p>Principal component analysis of miRNA microarray data confirmed that case and control groups were distinct, and biological repeats were homogeneous. After normalisation of chip data, differential miRNAs were screened using fold change and Student's <em>t</em>-tests; 77 miRNAs were up-regulated (fold change > 2), and 44 miRNAs were down-regulated (fold change < 0.5).</p></div><div><h3>Conclusion</h3><p>ICH patients display distinct miRNA expression profiles in CSF, suggesting that miRNAs may be auxiliary diagnostic biomarkers for ICH.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 4","pages":"Pages 141-145"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2019.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80316434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Application of terahertz spectroscopy in biomolecule detection 太赫兹光谱在生物分子检测中的应用

Frontiers in Laboratory Medicine Pub Date : 2018-12-01 DOI: 10.1016/j.flm.2019.05.001

Liu Wei , Liu Yu , Huang Jiaoqi , Huang Guorong , Zhang Yang , Fu Weiling

引用次数: 43

Study on the immunity protection of 14-3-3–MPLA–liposome vaccine against cystic echinococcosis in mice 14-3-3 - mpla -脂质体疫苗对小鼠囊性包虫病免疫保护作用的研究

Frontiers in Laboratory Medicine Pub Date : 2018-12-01 DOI: 10.1016/j.flm.2019.05.002

Li ZongJi, Ding Shuqing, Li Yuan

{"title":"Study on the immunity protection of 14-3-3–MPLA–liposome vaccine against cystic echinococcosis in mice","authors":"Li ZongJi, Ding Shuqing, Li Yuan","doi":"10.1016/j.flm.2019.05.002","DOIUrl":"10.1016/j.flm.2019.05.002","url":null,"abstract":"<div><p>The study aims to investigate the protective immunity against <em>Echinococcus granulosus</em> in mice immunized with14-3-3–MPLA–liposome vaccine. ICR (Institute of Cancer Research) mice were subcutaneously immunized three times with 14-3-3–MPLA–liposome vaccine, followed by the challenge with <em>Echinococcus granulosus</em> protoscoleces intraperitoneally and then sacrificed after six months of post-challenge. The levels of IL-4, and IFN-γ were measured by ELISA. The rate of reduced hydatid cyst and the levels of IgE, IgG and IgG subclasses in sera were examined. The result showed that mice vaccinated with14-3-3–MPLA–liposome vaccine and challenged with protoscoleces revealed significant protective immunity of 95.07%. ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a. The level of IFN-γ increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 levels between vaccinated and control mice. It is concluded that the14-3-3–MPLA–liposome vaccine could induce a high level of protective immunity to prevent cystic echinococcosis. The MPLA and liposome are promising vaccine adjuvant and delivery system.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 4","pages":"Pages 146-151"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2019.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85739157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Using digital PCR to detect HER2 amplification in breast and gastric cancer patients 应用数字PCR检测乳腺癌和胃癌患者的HER2扩增

Frontiers in Laboratory Medicine Pub Date : 2018-09-01 DOI: 10.1016/j.flm.2018.11.002

Ruifeng Zhou , Peng Yuan , Lihua Zhang , Shuangye Shen , Zhaoliang Li , Yan Wang

引用次数: 4

Laboratory diagnosis of antiphospholipid syndrome 抗磷脂综合征的实验室诊断

Frontiers in Laboratory Medicine Pub Date : 2018-09-01 DOI: 10.1016/j.flm.2018.08.003

Chao Wang , Xiu-ru Guan

引用次数: 2

Prevalence of hepatitis E virus infection among pregnant women in Zhenjiang, China 镇江市孕妇戊型肝炎病毒感染率调查

Frontiers in Laboratory Medicine Pub Date : 2018-09-01 DOI: 10.1016/j.flm.2018.11.003

Zhilian Rui , Lihua Jiang , Xiaochun Wang , Yan Yang , Ying Peng , Yu Ling , Wen Zhang , Xingli Fu , Chenglin Zhou , Shixing Yang , Quan Shen

{"title":"Prevalence of hepatitis E virus infection among pregnant women in Zhenjiang, China","authors":"Zhilian Rui , Lihua Jiang , Xiaochun Wang , Yan Yang , Ying Peng , Yu Ling , Wen Zhang , Xingli Fu , Chenglin Zhou , Shixing Yang , Quan Shen","doi":"10.1016/j.flm.2018.11.003","DOIUrl":"10.1016/j.flm.2018.11.003","url":null,"abstract":"<div><h3>Background</h3><p>Most hepatitis E virus (HEV) infections are mild or subclinical, however, the infections are characteristically associated with a high incidence of symptomatic presentation among pregnant women.</p></div><div><h3>Objectives</h3><p>The aim of this study was to assess the HEV prevalence among pregnant women in Zhenjiang, China.</p></div><div><h3>Materials and methods</h3><p>225 and 208 of serum samples collected from pregnant and healthy nonpregnant women, respectively, were used to detect anti-HEV IgG and IgM levels. IgM positive samples were further tested for HEV RNA by using RT-nested PCR (nPCR) method. Positive PCR products were sequenced and subjected to phylogenetic analysis.</p></div><div><h3>Results</h3><p>21.8% (49/225) of samples were anti-HEV IgG positive in pregnant women, whereas 7.7% (16/208) of samples were anti-HEV IgG positive in control subjects (<em>P</em> < 0.001). With respect to anti-HEV IgM testing, 3.6% (8/225) and 0.0% (0/208) were positive in pregnant women and control subjects, respectively (<em>P</em> = 0.006 < 0.01). 4 out of 8 IgM positive samples were positive for HEV RNA. Phylogenetic analysis indicated that four HEV strains had distinct nucleotide sequences and clustered into two different sub-genotypes in genotype 4.</p></div><div><h3>Conclusions</h3><p>In the current study, the prevalence of HEV infection with respect to IgG and IgM levels among pregnant women was significantly higher than that in the healthy nonpregnant group. Additionally, all four HEV strains belonged to genotype 4 implying that genotype 4 is the predominant genotype in the area.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 3","pages":"Pages 116-119"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.11.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75709545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Combined detection of erythrocyte and reticulocyte to improve screening efficiency of thalassaemia trait in pregnancy 红细胞与网织红细胞联合检测提高妊娠地中海贫血特征筛查效率

Frontiers in Laboratory Medicine Pub Date : 2018-09-01 DOI: 10.1016/j.flm.2018.12.001

Yuwei Yang , Jiafu Feng , Bi Peng , Xiaohong Chen , Jun Ying , Tao Yang

{"title":"Combined detection of erythrocyte and reticulocyte to improve screening efficiency of thalassaemia trait in pregnancy","authors":"Yuwei Yang , Jiafu Feng , Bi Peng , Xiaohong Chen , Jun Ying , Tao Yang","doi":"10.1016/j.flm.2018.12.001","DOIUrl":"10.1016/j.flm.2018.12.001","url":null,"abstract":"<div><h3>Objective</h3><p>Both iron deficiency anemia (IDA) and thalassaemia trait (TT) are indistinguishable in pregnancy. This study aimed to establish some new hematological indices based on erythrocyte and reticulocyte parameters to distinguish each other.</p></div><div><h3>Methods</h3><p>A total of 425 anemic pregnant women including 177 cases with IDA, 105 cases with α-TT, and 143 cases with β-TT were enrolled in the study and divided into two groups: development and validation groups. Logit-P1 (for identifying TT from IDA) and logit-P2 equations (for distinguishing between α-TT and β-TT) were designed in the development group, and their diagnostic performances were compared with 12 previous hematological indices in the validation group.</p></div><div><h3>Results</h3><p>The optimal cut-off value, sensitivity, sensitivity of logit-P1 were 0.84, 85.9%, 55.9%, and those of logit-P2 were 0.41, 85.9%, 55.9%. Compared to 12 hematological indices, logit-P1 (AUC = 0.765) and logit-P2 (AUC = 0.919) provided optimal identification performances within respective application ranges (P < 0.001). In combination with the supplementary criteria (RBC#≥4.1 × 10<sup>12</sup>/L), the two equations had low missed diagnosis rate and high accuracy rate for α-TT carriers (6.3% and 79.4%) and β-TT carriers (4.7% and 81.4%), and showed moderate agreement with clinical diagnosis (Kappa = 0.540).</p></div><div><h3>Conclusion</h3><p>For screening TT in anemic pregnant women, reticulocyte detection should be taken into account. The hematological indices based on erythrocyte and reticulocyte parameters would be superior to those only calculated by erythrocyte parameters.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 3","pages":"Pages 120-125"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74457270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Prognostic value of Bmi-1 oncoprotein expression in non-small cell lung cancer 非小细胞肺癌中Bmi-1癌蛋白表达的预后价值

Frontiers in Laboratory Medicine Pub Date : 2018-09-01 DOI: 10.1016/j.flm.2018.11.001

Dan Xiong , Yunlin Ye , Bohua Kuang , Hongmei Mo , Xiaoqing Xu , Qinquan Cai , Enyun Wang , Wensen Lu , Yanping Luo , Jiehong Wei , Xiuming Zhang

{"title":"Prognostic value of Bmi-1 oncoprotein expression in non-small cell lung cancer","authors":"Dan Xiong , Yunlin Ye , Bohua Kuang , Hongmei Mo , Xiaoqing Xu , Qinquan Cai , Enyun Wang , Wensen Lu , Yanping Luo , Jiehong Wei , Xiuming Zhang","doi":"10.1016/j.flm.2018.11.001","DOIUrl":"10.1016/j.flm.2018.11.001","url":null,"abstract":"<div><p>Background: In our prior study, Bmi-1 was elevated in non-small cell lung cancer (NSCLC) and took an important role in the early stage of progression. However, the clinical significance of Bmi-1 in NSCLC was uncertain. The aim of this study was to illuminate the relationship between Bmi-1, clinical variables and the prognosis of patients with NSCLC. Methods: Real-time PCR was used to examine the expression of Bmi-1 mRNA in 23 paired NSCLC and the adjacent normal tissues. The expression of Bmi-1 protein in 128 specimens of NSCLC was determined by immunohistochemistry assay. Statistical analyses were performed to evaluate the association between the expression of Bmi-1, clinicopathologic features and prognosis. Results: Up-regulated expression of Bmi-1 mRNA was observed in 91.3% of NSCLC (<em>P</em> < 0.0001). The level of Bmi-1 was positively correlated with T stage (<em>P</em> = 0.019), and the 5-year overall survival of patients with higher Bmi-1 level was lower than that of patients with lower Bmi-1 level without statistical significance (<em>P</em> = 0.189). Histological classification and smoking status were not related to prognosis (<em>P</em> = 0.166, 0.615). For patients with adenocarcinoma, lower level of Bmi-1 was related to good prognosis (<em>P</em> = 0.033), and in multivariate analysis, metastasis, T stage and lymph node involvement were independent prognostic factors (<em>P</em> = 0.001, 0.044, 0.013). Conclusions: Expression of Bmi-1 mRNA was higher in NSCLC than in the adjacent normal tissues. The detection of Bmi-1 protein expression is potentially useful in prognostic evaluation of lung adenocarcinoma.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 3","pages":"Pages 109-115"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84352294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A digital PCR assay development to detect EGFR T790M mutation in NSCLC patients 建立了一种检测非小细胞肺癌患者EGFR T790M突变的数字PCR方法

Frontiers in Laboratory Medicine Pub Date : 2018-09-01 DOI: 10.1016/j.flm.2018.08.002

Ruifeng Zhou , Yiran Cai , Zhaoliang Li , Shuangye Shen , Mozhou Sha , Steven R. Head , Yan Wang

{"title":"A digital PCR assay development to detect EGFR T790M mutation in NSCLC patients","authors":"Ruifeng Zhou , Yiran Cai , Zhaoliang Li , Shuangye Shen , Mozhou Sha , Steven R. Head , Yan Wang","doi":"10.1016/j.flm.2018.08.002","DOIUrl":"10.1016/j.flm.2018.08.002","url":null,"abstract":"<div><p>Epidermal growth factor receptor (EGFR) is a crucial factor in the development of non-small cell lung cancer (NSCLC). Tyrosine kinase inhibitor (TKI) therapy requires a sensitive and accurate assay for detection of drug resistant mutations in patients undergoing TKI-based targeted therapy. Digital polymerase chain reaction (PCR) is a highly sensitive PCR based detection method with the capability of absolute quantification. Here we present a digital PCR based liquid biopsy for EGFR T790M detection from blood samples with a low detection limit of 0.1% mutant allele frequency. The assay shows 100% concordance with test results obtained from cFDA approved ARMS PCR based EGFR mutation detection assay on FFPE tissue sections. It also demonstrated satisfactory sensitivity and specificity at 62.5% and 100% respectively. This assay can satisfy the clinical need of detecting EGFR T790M mutations at extremely low allele frequency. It can be used as both a companion diagnostic tool and a monitoring strategy for the development of drug resistance, and to evaluate therapeutic effects on disease progression.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 3","pages":"Pages 89-96"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2018.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88850636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10