Qinghai Shi , Di Ge , Qi Yang , Li Wang , Jianfeng Fu
{"title":"脑出血患者脑脊液MicroRNA谱分析","authors":"Qinghai Shi , Di Ge , Qi Yang , Li Wang , Jianfeng Fu","doi":"10.1016/j.flm.2019.07.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Differential expression profiles of free microRNAs (miRNAs) in cerebrospinal fluid (CSF) from patients with intracerebral haemorrhage (ICH) and controls were analysed to identify miRNA markers associated with ICH-induced brain injury.</p></div><div><h3>Methods</h3><p>Five patients with acute ICH were enrolled alongside five control inpatients without CNS diseases. CSF specimens were collected during initial lumbar puncture after admission, total free RNAs were extracted from CSF, and miRNAs were detected by Agilent Human miRNA V21.0 chip experiments. Differential expression profiles were statistically analysed.</p></div><div><h3>Results</h3><p>Principal component analysis of miRNA microarray data confirmed that case and control groups were distinct, and biological repeats were homogeneous. After normalisation of chip data, differential miRNAs were screened using fold change and Student's <em>t</em>-tests; 77 miRNAs were up-regulated (fold change > 2), and 44 miRNAs were down-regulated (fold change < 0.5).</p></div><div><h3>Conclusion</h3><p>ICH patients display distinct miRNA expression profiles in CSF, suggesting that miRNAs may be auxiliary diagnostic biomarkers for ICH.</p></div>","PeriodicalId":100555,"journal":{"name":"Frontiers in Laboratory Medicine","volume":"2 4","pages":"Pages 141-145"},"PeriodicalIF":0.0000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.flm.2019.07.001","citationCount":"5","resultStr":"{\"title\":\"MicroRNA profiling of cerebrospinal fluid from patients with intracerebral haemorrhage\",\"authors\":\"Qinghai Shi , Di Ge , Qi Yang , Li Wang , Jianfeng Fu\",\"doi\":\"10.1016/j.flm.2019.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Differential expression profiles of free microRNAs (miRNAs) in cerebrospinal fluid (CSF) from patients with intracerebral haemorrhage (ICH) and controls were analysed to identify miRNA markers associated with ICH-induced brain injury.</p></div><div><h3>Methods</h3><p>Five patients with acute ICH were enrolled alongside five control inpatients without CNS diseases. CSF specimens were collected during initial lumbar puncture after admission, total free RNAs were extracted from CSF, and miRNAs were detected by Agilent Human miRNA V21.0 chip experiments. Differential expression profiles were statistically analysed.</p></div><div><h3>Results</h3><p>Principal component analysis of miRNA microarray data confirmed that case and control groups were distinct, and biological repeats were homogeneous. After normalisation of chip data, differential miRNAs were screened using fold change and Student's <em>t</em>-tests; 77 miRNAs were up-regulated (fold change > 2), and 44 miRNAs were down-regulated (fold change < 0.5).</p></div><div><h3>Conclusion</h3><p>ICH patients display distinct miRNA expression profiles in CSF, suggesting that miRNAs may be auxiliary diagnostic biomarkers for ICH.</p></div>\",\"PeriodicalId\":100555,\"journal\":{\"name\":\"Frontiers in Laboratory Medicine\",\"volume\":\"2 4\",\"pages\":\"Pages 141-145\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.flm.2019.07.001\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Laboratory Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2542364919300135\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Laboratory Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2542364919300135","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
MicroRNA profiling of cerebrospinal fluid from patients with intracerebral haemorrhage
Objective
Differential expression profiles of free microRNAs (miRNAs) in cerebrospinal fluid (CSF) from patients with intracerebral haemorrhage (ICH) and controls were analysed to identify miRNA markers associated with ICH-induced brain injury.
Methods
Five patients with acute ICH were enrolled alongside five control inpatients without CNS diseases. CSF specimens were collected during initial lumbar puncture after admission, total free RNAs were extracted from CSF, and miRNAs were detected by Agilent Human miRNA V21.0 chip experiments. Differential expression profiles were statistically analysed.
Results
Principal component analysis of miRNA microarray data confirmed that case and control groups were distinct, and biological repeats were homogeneous. After normalisation of chip data, differential miRNAs were screened using fold change and Student's t-tests; 77 miRNAs were up-regulated (fold change > 2), and 44 miRNAs were down-regulated (fold change < 0.5).
Conclusion
ICH patients display distinct miRNA expression profiles in CSF, suggesting that miRNAs may be auxiliary diagnostic biomarkers for ICH.
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