A simple assay for direct colorimetric detection of prostatic acid phosphatase (PAP) at fg levels using biphosphonated loaded PEGylated gold nanoparticles

Frontiers in Laboratory Medicine Pub Date : 2017-12-01 DOI:10.1016/j.flm.2017.11.005

Maroua Ben Haddada , Fatima Aouidat , Maelle Monteil , Marc Lecouvey , Marc Lamy de la Chapelle , Jolanda Spadavecchia

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引用次数: 3

Abstract

A fast and sensitive colorimetric assay for the determination of prostatic acid phosphatase (PAP) levels using biphosphonates-PEG-coated gold nanoparticles (BP-PEG-AuNPs) has been developed. Addition of different amounts of PAP to the AuNPs results in a significant change of color, from a red-wine color to purple, and finally to blue. The PAP should bind onto the AuNPs surface and crosslink the AuNPs inducing their aggregation and as a consequence a color change of the solution from wine red to purple/blue. The color change was monitored by UV–vis spectrophotometry or with the naked eye. The ensuing concentration-dependent red-shift of surface plasmon resonance absorption allows the determination concentration within the fg range in 10 min. Importantly, the visual detection limit (1 ng/mL) allows the rapid differential diagnosis with naked eye or image analyzer. This demonstrates that the color modification observed with BP-PEG-AuNP is effectively due to an interaction between PAP and BP.

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一种使用双磷酸负载聚乙二醇化金纳米颗粒直接比色法检测前列腺酸性磷酸酶(PAP)在fg水平的简单方法

建立了一种快速灵敏的双膦酸盐-聚乙二醇包覆金纳米颗粒(BP-PEG-AuNPs)测定前列腺酸性磷酸酶(PAP)水平的比色法。向aunp中添加不同量的PAP会导致颜色发生显著变化，从红酒色到紫色，最后变为蓝色。PAP应结合到AuNPs表面并与AuNPs交联，从而诱导其聚集，从而使溶液的颜色从酒红色变为紫色/蓝色。用紫外-可见分光光度法或肉眼监测颜色变化。随后的表面等离子体共振吸收的浓度依赖红移允许在10 min内测定fg范围内的浓度。重要的是，视觉检测限(1 ng/mL)允许用肉眼或图像分析仪快速鉴别诊断。这表明，用BP- peg - aunp观察到的颜色修饰是由于PAP和BP之间的相互作用而有效的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Frontiers in Laboratory Medicine

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