Engineering Microbiology最新文献_第2页

Characterization of Bacillus subtilis phages PJNB030, PJNB031, and PJNB032 reveals wall teichoic acid as a key receptor determinant 枯草芽孢杆菌噬菌体PJNB030、PJNB031和PJNB032的鉴定表明壁磷壁酸是一个关键的受体决定因素

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-19 DOI: 10.1016/j.engmic.2025.100253

Jiaoyang Song , Ming Hu , Min Zhao , Shengqing Luo , Yibao Chen , Yuqing Liu , Yingjun Li

{"title":"Characterization of Bacillus subtilis phages PJNB030, PJNB031, and PJNB032 reveals wall teichoic acid as a key receptor determinant","authors":"Jiaoyang Song , Ming Hu , Min Zhao , Shengqing Luo , Yibao Chen , Yuqing Liu , Yingjun Li","doi":"10.1016/j.engmic.2025.100253","DOIUrl":"10.1016/j.engmic.2025.100253","url":null,"abstract":"<div><div><em>Bacillus subtilis</em> is widely used in industrial fermentation and probiotic applications; however, phage contamination poses a substantial economic threat. To address this, we isolated three phages (PJNB030, PJNB031, and PJNB032) from a contaminated <em>B. subtilis</em> fermentation broth and characterized their biological properties. Phenotypic analyses indicated broad pH stability (pH 4–10), variable thermal tolerance, and differential UV sensitivity. Replication kinetics revealed latent periods of 10–20 min and burst sizes ranging from 50 to 73 PFU/cell. Genomic sequencing identified linear dsDNA genomes (64–165 kb) with GC content ranging from 33.5 to 47.4%. Phylogenomic and comparative genomic analyses revealed that these phages were located on distinct branches. Deletion of <em>pgcA</em> (which encodes α-phosphoglucomutase) rendered cells completely resistant to PJNB031 and PJNB032, whereas it reduced PJNB030 infectivity (plaque formation efficiency) by approximately six orders of magnitude. Adsorption assays confirmed that the binding of PJNB031 and PJNB032 to Δ<em>pgcA</em> mutants was abolished, whereas PJNB030 retained partial adsorption capacity. In conclusion, this study identified wall teichoic acid as the primary receptor for these phages and established <em>pgcA</em> deletion as an effective strategy for engineering phage-resistant <em>B. subtilis</em> strains. Our findings provide critical insights into the mitigation of phage contamination in industrial bioprocesses.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100253"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145928176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically encoded biosensors enabled high-throughput screening of microbial cell factories 基因编码的生物传感器实现了微生物细胞工厂的高通量筛选

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-24 DOI: 10.1016/j.engmic.2025.100258

Jin Wang , Xueyan Liu , Longqian Zhao , Yue Zhang , Meng Wang

{"title":"Genetically encoded biosensors enabled high-throughput screening of microbial cell factories","authors":"Jin Wang , Xueyan Liu , Longqian Zhao , Yue Zhang , Meng Wang","doi":"10.1016/j.engmic.2025.100258","DOIUrl":"10.1016/j.engmic.2025.100258","url":null,"abstract":"<div><div>Genetically encoded biosensors provide powerful tools for coupling desired phenotypes to detectable outputs and have been extensively developed to detect a wide range of natural and unnatural products. When integrated with diverse high-throughput screening (HTS) approaches, these biosensors enable efficient product-driven screening across various throughputs, thereby expediting the engineering and optimization of microbial cell factories to produce various target compounds. For effective HTS of microbial cell factories, biosensors need to possess certain crucial characteristics. The performance features of biosensors significantly influence their application potential in HTS and can be precisely engineered through synthetic biology strategies. Furthermore, to ensure biosensor-driven HTS, additional engineering and optimizations of the biosensors are often required to increase the success rate and reduce false positives in the screening process. This review discusses the essential features of genetically encoded biosensors designed for HTS and then summarizes the latest advances in biosensor engineering for HTS purposes via synthetic biology strategies. Following this, the challenges and optimization of biosensors to adapt to different HTS processes are also discussed and exemplified. Finally, the key concerns and research prospects of developing biosensors for HTS applications are highlighted. Overall, this review provides comprehensive guidance on the engineering of genetically encoded biosensors and their applications in HTS for developing microbial cell factories to produce diverse target compounds.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100258"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in microbial mevalonolactone production: from fermentative mevalonate accumulation to downstream lactonization 微生物甲羟戊酸内酯生产的进展：从发酵甲羟戊酸积累到下游内酯化

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-24 DOI: 10.1016/j.engmic.2025.100257

Hao Tang , Lihong Yin , Yiwen Jiang , Teng Xu , Ting Xue , Meimei Fu , Jianghao Chen , Jinshan Guo

引用次数: 0

Heterologous expression of Vibrio natriegens transporters enhances the growth of Escherichia coli and Bacillus subtilis on glutamate 产氮弧菌转运体的异源表达促进了大肠杆菌和枯草芽孢杆菌在谷氨酸上的生长

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2026-02-17 DOI: 10.1016/j.engmic.2026.100264

Fei Han , Qian Wang , Yiheng Wang, Xingyu Liu, Yifan Zhang, Kunlong Liang, Xiongfeng Dai, Manlu Zhu

引用次数: 0

Diversity analysis of phytase-producing bacteria from coastal seawater and sediment and characterization of their phytases 沿海海水和沉积物中产植酸菌的多样性分析及其产植酸酶的特性

Engineering Microbiology Pub Date : 2025-12-01 Epub Date: 2025-07-03 DOI: 10.1016/j.engmic.2025.100223

Xiao-Jie Yuan , Rui Liu , Jian Li , Wen-Xiao Zhao , Hui-Hui Fu , Yan-Rong Zhou , Mei-Ling Sun , Xiu-Lan Chen , Yu-Qiang Zhang

{"title":"Diversity analysis of phytase-producing bacteria from coastal seawater and sediment and characterization of their phytases","authors":"Xiao-Jie Yuan , Rui Liu , Jian Li , Wen-Xiao Zhao , Hui-Hui Fu , Yan-Rong Zhou , Mei-Ling Sun , Xiu-Lan Chen , Yu-Qiang Zhang","doi":"10.1016/j.engmic.2025.100223","DOIUrl":"10.1016/j.engmic.2025.100223","url":null,"abstract":"<div><div>Phytic acid, also known as inositol hexaphosphate (IP6), is one of the most abundant organophosphorus compounds in nature. Its degradation by phytase plays a key role in the natural phosphorus cycle. In addition, phytases are widely used in livestock and poultry feed to enhance phosphorus utilization. While most reported and commercial phytases are derived from terrestrial organisms, relatively few originate from marine microorganisms, and information on the diversity of phytase-producing marine bacteria remains limited. In this study, following enrichment with sodium phytate, we analyzed the bacterial diversity in seawater and sediment samples collected from the coast of Aoshan Bay in Qingdao, China, using 16S rRNA gene amplicon sequencing. A total of 138 OTUs representing 10 phyla, 15 classes, 37 orders, 55 families, and 70 genera were identified. Furthermore, 27 phytase-producing bacterial strains were isolated from the enrichment cultures, primarily belonging to the phyla Firmicutes (14/27) and Proteobacteria (12/27). Five extracellular phytase genes were identified through genome sequencing of three representative strains. These phytases were subsequently expressed and characterized. All were classified as histidine acid phosphatase-type phytases, exhibiting optimal activity at temperatures of 50–60 °C and pH values of 4.0–5.0. Notably, phytase 3919 showed a specific activity as high as 2485.25 U/mg, indicating strong potential for practical applications. This study provides insight into the diversity of coastal bacteria involved in phytic acid degradation, contributing to our understanding of bacterial-driven phosphorus cycling in coastal ecosystems and facilitating the discovery of phytases with industrial potential.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100223"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterization of a potential l-Homoserine exporter in Corynebacterium glutamicum 谷氨酸棒状杆菌l-同型丝氨酸潜在输出菌的鉴定和功能表征

Engineering Microbiology Pub Date : 2025-12-01 Epub Date: 2025-09-13 DOI: 10.1016/j.engmic.2025.100240

Xiaodi Liu , Xiangyu Zhu , Wenxin Jiang , Huanmin Du

{"title":"Identification and functional characterization of a potential l-Homoserine exporter in Corynebacterium glutamicum","authors":"Xiaodi Liu , Xiangyu Zhu , Wenxin Jiang , Huanmin Du","doi":"10.1016/j.engmic.2025.100240","DOIUrl":"10.1016/j.engmic.2025.100240","url":null,"abstract":"<div><div>Exporter protein systems play a crucial role in the efficient production of valuable chemicals. However, the lack of active exporters significantly limits the application of industrial bio-based production, making the identification and utilization of novel exporters highly important. In this study, we discovered a novel <span>l</span>-Homoserine exporter, Cg0701, in <em>Corynebacterium glutamicum</em> through homology analysis. First, tolerance assays revealed that the <em>cg0701</em> overexpression strain (CgH-2) exhibited a 10.45% increase in cell growth compared to the control when cultivated with 30 g/L-Homoserine. Additionally, export assays demonstrated that the <span>l</span>-Homoserine export capacity of CgH-2 increased by approximately 30%. Furthermore, genomic overexpression of <em>cg0701</em> in an <span>l</span>-Homoserine-producing chassis also enhanced both tolerance and export activity. As a result, the recombinant strain CgH-11 produced 10.79 g/L-Homoserine in shake flask cultures and 48.72 g/L in a 5 L fermenter, representing improvements of 19.89% and 24.44%, respectively. In summary, our results indicate that Cg0701 is a novel <span>l</span>-Homoserine exporter in <em>C. glutamicum</em>, enriching our understanding of amino acid export systems and providing a valuable target for the construction of <span>l</span>-Homoserine microbial cell factories.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145121025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Archaeasins as a promising resource for developing next-generation antibiotics uncovered via deep learning 通过深度学习发现的古菌原是开发下一代抗生素的有前途的资源

Engineering Microbiology Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.engmic.2025.100241

Huan Du, Yang Liu

引用次数: 0

Electricity generation and dibenzothiophene biodegradation using a novel electroactive bacterium Lysinibacillus macroides AP in microbial fuel cells 在微生物燃料电池中利用一种新型电活性细菌大内溶杆菌AP发电和降解二苯并噻吩

Engineering Microbiology Pub Date : 2025-12-01 Epub Date: 2025-06-23 DOI: 10.1016/j.engmic.2025.100221

Zihan Huang, Lei Zhang, Ting Cai, Ruijun Liu, Xiaoyan Qi, Xia Wang

{"title":"Electricity generation and dibenzothiophene biodegradation using a novel electroactive bacterium Lysinibacillus macroides AP in microbial fuel cells","authors":"Zihan Huang, Lei Zhang, Ting Cai, Ruijun Liu, Xiaoyan Qi, Xia Wang","doi":"10.1016/j.engmic.2025.100221","DOIUrl":"10.1016/j.engmic.2025.100221","url":null,"abstract":"<div><div>Polycyclic aromatic sulfur heterocycles, such as dibenzothiophene (DBT), and their alkylated derivatives are recognized as persistent and toxic contaminants that pose major risks to the environment and human health. Here, a novel electroactive gram-positive bacterium, <em>Lysinibacillus macroides</em> AP, was isolated and identified from a microbial fuel cell (MFC) powered by aromatic compounds. An electricity generation performance with a maximum discharge voltage of 424.59 mV and a power density of 420.95 mW m⁻<sup>2</sup> was obtained using <em>L. macroides</em> AP in an MFC fueled with sodium formate. An analysis of the extracellular electron transfer (EET) mechanism indicated that the endogenous redox mediators produced by <em>L. macroides</em> AP were not detected, but exogenous redox mediators such as thionine acetate and anthraquinone-2, 6-disulfonate could temporarily enhance EET. The characterization of biofilm morphology revealed a dense network of microbial nanowires on the cell surface of <em>L. macroides</em> AP; the abundance of these nanowires was positively correlated with the discharge efficiency of the MFC, suggesting that the nanowires generated by <em>L. macroides</em> AP cells were likely to promote EET. Additionally, effective bioelectricity generation and simultaneous DBT degradation were successfully achieved using <em>L. macroides</em> AP in MFCs, with a power density of 385.20 mW m⁻<sup>2</sup> and 88.72 % DBT removal. This is the first report on a novel ecological role of <em>L. macroides</em> AP as a gram-positive electroactive bacterium, emphasizing its potential applications in environmental remediation and energy recovery.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100221"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifidobacterium animalis subsp. lactis genome resources and metabolite profiling at the strain level and their ability to alleviate anxiety-like behavior in a sleep-deprived mouse model 动物双歧杆菌亚种。Lactis基因组资源和代谢产物分析在应变水平及其缓解睡眠剥夺小鼠模型中焦虑样行为的能力

Engineering Microbiology Pub Date : 2025-12-01 Epub Date: 2025-07-30 DOI: 10.1016/j.engmic.2025.100228

Yao-Kun Zhang , Liang Zhang , Xue Ni , Shu-Wen Zhang , Min-Zhi Jiang , Si-Lu Zhang , Guo-Xun Xiao , He Jiang , Ming-Xia Bi , Yu-Lin Wang , Chang Liu , Shuang-Jiang Liu

{"title":"Bifidobacterium animalis subsp. lactis genome resources and metabolite profiling at the strain level and their ability to alleviate anxiety-like behavior in a sleep-deprived mouse model","authors":"Yao-Kun Zhang , Liang Zhang , Xue Ni , Shu-Wen Zhang , Min-Zhi Jiang , Si-Lu Zhang , Guo-Xun Xiao , He Jiang , Ming-Xia Bi , Yu-Lin Wang , Chang Liu , Shuang-Jiang Liu","doi":"10.1016/j.engmic.2025.100228","DOIUrl":"10.1016/j.engmic.2025.100228","url":null,"abstract":"<div><div><em>Bifidobacterium animalis</em> subsp. <em>lactis</em> is a well-known probiotic with potential benefits for alleviating sub-health symptoms, including immune dysfunction and anxiety. Given the strain-specific nature of its probiotic effects, identifying effective strains for sub-health alleviation is crucial. In this study, we characterized 16 <em>B animalis</em> subsp. <em>lactis</em> isolates from fecal samples and probiotic sources. We assessed the genotype-phenotype correlations related to growth, carbon source utilization, and stress tolerance <em>in vitro</em>. Subsequently, we profiled 107 metabolites (including 28 alcohols and 17 esters) and quantified the levels of short-chain fatty acids and three other organic acids. Three <em>B. animalis</em> strains, GOLDGUT-BB21, WLBA7, and WLBA6, were selected and evaluated in a sleep-deprived mouse model. <em>In vivo</em>, WLBA3 reduced inflammation and oxidative stress by inhibiting the NLRP3 inflammasome pathway and modulating gut microbiota (e.g., <em>Lactobacillus</em> and <em>Alistipes</em>), which in turn significantly improved weight gain and fatigue resistance, attenuated cognitive function, and anxiety-like behavior. These findings provide insights into the diversity of <em>B. animalis</em> subsp. <em>lactis</em> strain resources and highlight the potential of WLBA3 as a candidate for alleviating sub-health symptoms.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100228"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systems metabolic engineering of glutathione biosynthesis in Saccharomyces cerevisiae: Pathway balancing coupled with enzyme screening for high-titer production 酿酒酵母谷胱甘肽生物合成的系统代谢工程：高滴度生产的途径平衡与酶筛选

Engineering Microbiology Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.engmic.2025.100243

Zhiqi Hu , Mengyuan Su , Qibing Liu , Ying Li , Yunxiang Liang , Shuangquan Li , Yingjun Li

{"title":"Systems metabolic engineering of glutathione biosynthesis in Saccharomyces cerevisiae: Pathway balancing coupled with enzyme screening for high-titer production","authors":"Zhiqi Hu , Mengyuan Su , Qibing Liu , Ying Li , Yunxiang Liang , Shuangquan Li , Yingjun Li","doi":"10.1016/j.engmic.2025.100243","DOIUrl":"10.1016/j.engmic.2025.100243","url":null,"abstract":"<div><div>Glutathione (GSH), an essential tripeptide thiol critical for cellular redox regulation, has significant value in the pharmaceutical and nutraceutical industries. To overcome limitations of traditional GSH extraction methods, this study established a microbial cell factory platform in <em>Saccharomyces cerevisiae</em> through integrated metabolic engineering strategies: (1) host strain screening identified NJ-SQYY with superior GSH accumulation (74.14 mg·L⁻¹, 8.27 mg·g<sup>-1</sup> dry cell weight [DCW]); (2) CRISPR/Cas9-mediated genomic integration of bacterial <em>gshAB</em> introduced with a bifunctional glutathione synthetase; (3) systematic optimization via promoter tuning and Gsh1-Gsh2 enzyme fusion, and <em>CYS3</em> overexpression-resolved γ-glutamylcysteine bottlenecks. These interventions synergistically enhanced GSH synthesis to 339.3 mg·L⁻¹ in shake flasks (4.6-fold increase), representing the highest reported titer in chromosomally engineered <em>S. cerevisiae</em>. Scaling to dissolved oxygen-coupled fed-batch fermentation in a 5-L bioreactor produced 997.46 mg·L⁻¹ GSH at 33.85 mg·g⁻¹ DCW. This study demonstrated a holistic metabolic engineering-to-bioprocessing approach for industrial GSH biomanufacturing.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100243"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0