Engineering Microbiology最新文献_第2页

The way to uncovering and utilizing marine microbial resources 发掘和利用海洋微生物资源之路

Engineering Microbiology Pub Date : 2024-09-24 DOI: 10.1016/j.engmic.2024.100175

Zhi-Feng Zhang, Meng Li

引用次数: 0

Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing 通过基于嗜热菌的综合生物加工技术利用木质纤维素生产生物燃料

Engineering Microbiology Pub Date : 2024-09-10 DOI: 10.1016/j.engmic.2024.100174

Yilin Le , Mengqi Zhang , Pengju Wu , Huilei Wang , Jinfeng Ni

{"title":"Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing","authors":"Yilin Le , Mengqi Zhang , Pengju Wu , Huilei Wang , Jinfeng Ni","doi":"10.1016/j.engmic.2024.100174","DOIUrl":"10.1016/j.engmic.2024.100174","url":null,"abstract":"<div><div>The depletion of fossil fuels and their impact on the environment have led to efforts to develop alternative sustainable fuels. While biofuel derived from lignocellulose is considered a sustainable, renewable, and green energy source, enhancing biofuel production and achieving a cost-effective bioconversion of lignocellulose at existing bio-refineries remains a challenge. Consolidated bioprocessing (CBP) using thermophiles can simplify this operation by integrating multiple processes, such as hydrolytic enzyme production, lignocellulose degradation, biofuel fermentation, and product distillation. This paper reviews recent developments in the conversion of lignocellulose to biofuel using thermophile-based CBP. First, advances in thermostable enzyme and thermophilic lignocellulolytic microorganism discovery and development for lignocellulosic biorefinery use are outlined. Then, several thermophilic CBP candidates and thermophilic microbes engineered to drive CBP of lignocellulose are reviewed. CRISPR/Cas-based genome editing tools developed for thermophiles are also highlighted. The potential applications of the Design-Build-Test-Learn (DBTL) synthetic biology strategy for designing and constructing thermophilic CBP hosts are also discussed in detail. Overall, this review illustrates how to develop highly sophisticated thermophilic CBP hosts for use in lignocellulosic biorefinery applications.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100174"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically engineered bacteria as inflammatory bowel disease therapeutics 作为炎症性肠病疗法的基因工程细菌

Engineering Microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.engmic.2024.100167

Zhen-Ping Zou , Xiao-Peng Zhang , Qian Zhang, Bin-Cheng Yin, Ying Zhou, Bang-Ce Ye

引用次数: 0

Mechanisms of tigecycline resistance in Gram-negative bacteria: A narrative review 革兰氏阴性细菌对替加环素产生耐药性的机制：叙述性综述

Engineering Microbiology Pub Date : 2024-08-17 DOI: 10.1016/j.engmic.2024.100165

Wenya Su , Wenjia Wang , Ling Li , Mengge Zhang , Hai Xu , Chengzhang Fu , Xiuhua Pang , Mingyu Wang

引用次数: 0

Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439 委内瑞拉链霉菌 ATCC 15439 整个 CYPome 和 Fdxome 的功能分析

Engineering Microbiology Pub Date : 2024-08-13 DOI: 10.1016/j.engmic.2024.100166

Shuai Li , Zhong Li , Guoqiang Zhang , Vlada B. Urlacher , Li Ma , Shengying Li

{"title":"Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439","authors":"Shuai Li , Zhong Li , Guoqiang Zhang , Vlada B. Urlacher , Li Ma , Shengying Li","doi":"10.1016/j.engmic.2024.100166","DOIUrl":"10.1016/j.engmic.2024.100166","url":null,"abstract":"<div><p>Cytochrome P450 enzymes (CYPs or P450s) and ferredoxins (Fdxs) are ubiquitously distributed in all domains of life. Bacterial P450s are capable of catalyzing various oxidative reactions with two electrons usually donated by Fdxs. Particularly in <em>Streptomyces</em>, there are abundant P450s that have exhibited outstanding biosynthetic capacity of bioactive metabolites and great potential for xenobiotic metabolisms. However, no systematic study has been conducted on physiological functions of the whole cytochrome P450 complement (CYPome) and ferredoxin complement (Fdxome) of any <em>Streptomyces</em> strain to date<em>,</em> leaving a significant knowledge gap in microbial functional genomics. Herein, we functionally analyze the whole CYPome and Fdxome of <em>Streptomyces venezuelae</em> ATCC 15439 by investigating groups of single and sequential P450 deletion mutants, single P450 overexpression mutants, and Fdx gene deletion or repression mutants. Construction of an unprecedented P450-null mutant strain indicates that none of P450 genes are essential for <em>S. venezuelae</em> in maintaining its survival and normal morphology. The non-housekeeping Fdx1 and housekeeping Fdx3 not only jointly support the cellular activity of the prototypic P450 enzyme PikC, but also play significant regulatory functions. These findings significantly advance the understandings of the native functionality of P450s and Fdxs as well as their cellular interactions.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100166"},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000286/pdfft?md5=a8270b363730ae79371412a1a3077ca0&pid=1-s2.0-S2667370324000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system 瘤胃分离出的皮氏不动杆菌 ROBY 的纤维素分解特性及潜在控释给药系统的设计

Engineering Microbiology Pub Date : 2024-08-03 DOI: 10.1016/j.engmic.2024.100164

Ruken Sariboga , Omer Faruk Sarioglu

{"title":"Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system","authors":"Ruken Sariboga , Omer Faruk Sarioglu","doi":"10.1016/j.engmic.2024.100164","DOIUrl":"10.1016/j.engmic.2024.100164","url":null,"abstract":"<div><p>A novel cellulolytic bacterial strain, ROBY, was isolated from a bovine rumen sample using the enrichment culture method. This isolate was found to be <em>Acinetobacter pittii</em>, with >99 % similarity according to 16S rRNA gene sequence analysis. The potential use of this strain in combination with doxorubicin (Dox)-integrated cellulose nanoparticles (Dox-CNPs) was evaluated as a proof-of-concept study for the further development of this approach as a novel controlled-release drug delivery strategy. The isolate can utilize CNPs as the sole carbon source for growth and degrade both Dox-CNPs and empty CNPs with high efficiency. Extracellular cellulases isolated from bacteria may also be used to trigger Dox release. The results also demonstrated that the release of Dox into the environment due to nanoparticle degradation in the samples incubated with Dox-CNPs significantly affected bacterial cell viability (∼75 % decrease), proving the release of Dox due to bacterial cellulase activity and suggesting the great potential of this approach for further development.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100164"},"PeriodicalIF":0.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000262/pdfft?md5=154741f24ca8241e1873123c2ea33593&pid=1-s2.0-S2667370324000262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications Paenibacillus elgii 上清液对筛选具有生物技术应用潜力的细菌菌株的影响

Engineering Microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.engmic.2024.100163

I. C． Cunha-Ferreira , C. S． Vizzotto , T. D． Frederico , J． Peixoto , L. S Carvalho , M. R． Tótola , R. H． Krüger

{"title":"Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications","authors":"I. C． Cunha-Ferreira , C. S． Vizzotto , T. D． Frederico , J． Peixoto , L. S Carvalho , M. R． Tótola , R. H． Krüger","doi":"10.1016/j.engmic.2024.100163","DOIUrl":"10.1016/j.engmic.2024.100163","url":null,"abstract":"<div><p>The biotechnological industry faces a crucial demand for novel bioactive compounds, particularly antimicrobial agents, to address the rising challenge of bacterial resistance to current available antibiotics. Traditional strategies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial producers. This study presents a protocol for targeted selection of bacterial strains using the supernatant of <em>Paenibacillus elgii</em>, which produces abundant signal molecules and antimicrobial peptides. Soil samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant, indicating their potential to produce similar compounds. The bacterial strains isolated through this method were assessed for their antibacterial activity. In addition, the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological interest. This study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100163"},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000250/pdfft?md5=3320eb58e754f7bdc2a2f5525731fd2b&pid=1-s2.0-S2667370324000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines 蛋白质热稳定性的调节及其在开发减温疫苗中的潜在应用

Engineering Microbiology Pub Date : 2024-06-25 DOI: 10.1016/j.engmic.2024.100162

Maofeng Wang , Cancan Wu , Nan Liu , Xiaoqiong Jiang , Hongjie Dong , Shubao Zhao , Chaonan Li , Sujuan Xu , Lichuan Gu

{"title":"Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines","authors":"Maofeng Wang , Cancan Wu , Nan Liu , Xiaoqiong Jiang , Hongjie Dong , Shubao Zhao , Chaonan Li , Sujuan Xu , Lichuan Gu","doi":"10.1016/j.engmic.2024.100162","DOIUrl":"10.1016/j.engmic.2024.100162","url":null,"abstract":"<div><p>The coronavirus disease 2019 (COVID-19) pandemic has highlighted the importance of developing novel vaccines. An ideal vaccine should trigger an intense immune reaction without causing significant side effects. In this study we found that substitution of tryptophan located in the cores of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) protein structures with certain smaller amino acids resulted in variants with melting temperatures of 33–37 °C. An enzyme activity assay indicated that the proteolytic activity of the main proteinase (3CL<sup>pro</sup>) decreased sharply when the environmental temperature exceeded the melting temperature, implying that other protein variants may lose most of their functions under the same conditions. This finding suggests that a virus variant containing engineered proteins with melting temperatures of 33–37 °C may only be functional in the upper respiratory tract where the temperature is about 33 °C, but will be unable to invade internal organs, which maintain temperatures above 37 °C, thus making it possible to construct temperature-sensitive attenuated vaccines.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100162"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000249/pdfft?md5=678b37931cc38b0eac77aed4ffe7562b&pid=1-s2.0-S2667370324000249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141961076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual role of phage terminase in Salmonella enterica oxidative stress response 噬菌体终结酶在肠炎沙门氏菌氧化应激反应中的双重作用

Engineering Microbiology Pub Date : 2024-06-04 DOI: 10.1016/j.engmic.2024.100156

Senfeng Zhang , Shengsheng Ma , Feizuo Wang , Chunyi Hu

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An upgraded Myxococcus xanthus chassis with enhanced growth characteristics for efficient genetic manipulation 具有增强生长特性的升级版黄腐霉菌底盘，可用于高效遗传操作

Engineering Microbiology Pub Date : 2024-06-03 DOI: 10.1016/j.engmic.2024.100155

Wei-feng Hu, Yan Wang, Xiao-ran Yue, Wei-wei Xue, Wei Hu, Xin-jing Yue, Yue-Zhong Li

引用次数: 0