Engineering Microbiology最新文献_第3页

Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system 瘤胃分离出的皮氏不动杆菌 ROBY 的纤维素分解特性及潜在控释给药系统的设计

Engineering Microbiology Pub Date : 2024-08-03 DOI: 10.1016/j.engmic.2024.100164

Ruken Sariboga , Omer Faruk Sarioglu

{"title":"Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system","authors":"Ruken Sariboga , Omer Faruk Sarioglu","doi":"10.1016/j.engmic.2024.100164","DOIUrl":"10.1016/j.engmic.2024.100164","url":null,"abstract":"<div><p>A novel cellulolytic bacterial strain, ROBY, was isolated from a bovine rumen sample using the enrichment culture method. This isolate was found to be <em>Acinetobacter pittii</em>, with >99 % similarity according to 16S rRNA gene sequence analysis. The potential use of this strain in combination with doxorubicin (Dox)-integrated cellulose nanoparticles (Dox-CNPs) was evaluated as a proof-of-concept study for the further development of this approach as a novel controlled-release drug delivery strategy. The isolate can utilize CNPs as the sole carbon source for growth and degrade both Dox-CNPs and empty CNPs with high efficiency. Extracellular cellulases isolated from bacteria may also be used to trigger Dox release. The results also demonstrated that the release of Dox into the environment due to nanoparticle degradation in the samples incubated with Dox-CNPs significantly affected bacterial cell viability (∼75 % decrease), proving the release of Dox due to bacterial cellulase activity and suggesting the great potential of this approach for further development.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100164"},"PeriodicalIF":0.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000262/pdfft?md5=154741f24ca8241e1873123c2ea33593&pid=1-s2.0-S2667370324000262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications Paenibacillus elgii 上清液对筛选具有生物技术应用潜力的细菌菌株的影响

Engineering Microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.engmic.2024.100163

I. C． Cunha-Ferreira , C. S． Vizzotto , T. D． Frederico , J． Peixoto , L. S Carvalho , M. R． Tótola , R. H． Krüger

{"title":"Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications","authors":"I. C． Cunha-Ferreira , C. S． Vizzotto , T. D． Frederico , J． Peixoto , L. S Carvalho , M. R． Tótola , R. H． Krüger","doi":"10.1016/j.engmic.2024.100163","DOIUrl":"10.1016/j.engmic.2024.100163","url":null,"abstract":"<div><p>The biotechnological industry faces a crucial demand for novel bioactive compounds, particularly antimicrobial agents, to address the rising challenge of bacterial resistance to current available antibiotics. Traditional strategies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial producers. This study presents a protocol for targeted selection of bacterial strains using the supernatant of <em>Paenibacillus elgii</em>, which produces abundant signal molecules and antimicrobial peptides. Soil samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant, indicating their potential to produce similar compounds. The bacterial strains isolated through this method were assessed for their antibacterial activity. In addition, the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological interest. This study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100163"},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000250/pdfft?md5=3320eb58e754f7bdc2a2f5525731fd2b&pid=1-s2.0-S2667370324000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines 蛋白质热稳定性的调节及其在开发减温疫苗中的潜在应用

Engineering Microbiology Pub Date : 2024-06-25 DOI: 10.1016/j.engmic.2024.100162

Maofeng Wang , Cancan Wu , Nan Liu , Xiaoqiong Jiang , Hongjie Dong , Shubao Zhao , Chaonan Li , Sujuan Xu , Lichuan Gu

{"title":"Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines","authors":"Maofeng Wang , Cancan Wu , Nan Liu , Xiaoqiong Jiang , Hongjie Dong , Shubao Zhao , Chaonan Li , Sujuan Xu , Lichuan Gu","doi":"10.1016/j.engmic.2024.100162","DOIUrl":"10.1016/j.engmic.2024.100162","url":null,"abstract":"<div><p>The coronavirus disease 2019 (COVID-19) pandemic has highlighted the importance of developing novel vaccines. An ideal vaccine should trigger an intense immune reaction without causing significant side effects. In this study we found that substitution of tryptophan located in the cores of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) protein structures with certain smaller amino acids resulted in variants with melting temperatures of 33–37 °C. An enzyme activity assay indicated that the proteolytic activity of the main proteinase (3CL<sup>pro</sup>) decreased sharply when the environmental temperature exceeded the melting temperature, implying that other protein variants may lose most of their functions under the same conditions. This finding suggests that a virus variant containing engineered proteins with melting temperatures of 33–37 °C may only be functional in the upper respiratory tract where the temperature is about 33 °C, but will be unable to invade internal organs, which maintain temperatures above 37 °C, thus making it possible to construct temperature-sensitive attenuated vaccines.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100162"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000249/pdfft?md5=678b37931cc38b0eac77aed4ffe7562b&pid=1-s2.0-S2667370324000249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141961076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual role of phage terminase in Salmonella enterica oxidative stress response 噬菌体终结酶在肠炎沙门氏菌氧化应激反应中的双重作用

Engineering Microbiology Pub Date : 2024-06-04 DOI: 10.1016/j.engmic.2024.100156

Senfeng Zhang , Shengsheng Ma , Feizuo Wang , Chunyi Hu

引用次数: 0

An upgraded Myxococcus xanthus chassis with enhanced growth characteristics for efficient genetic manipulation 具有增强生长特性的升级版黄腐霉菌底盘，可用于高效遗传操作

Engineering Microbiology Pub Date : 2024-06-03 DOI: 10.1016/j.engmic.2024.100155

Wei-feng Hu, Yan Wang, Xiao-ran Yue, Wei-wei Xue, Wei Hu, Xin-jing Yue, Yue-Zhong Li

引用次数: 0

A novel strategy to protect prokaryotic cells from virus infection 保护原核细胞免受病毒感染的新策略

Engineering Microbiology Pub Date : 2024-06-01 DOI: 10.1016/j.engmic.2024.100153

Yoshizumi Ishino

引用次数: 0

CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids 基于 CRISPR-Cas9 的基因组编辑技术在生产植物源萜类化合物的细菌工程中的应用

Engineering Microbiology Pub Date : 2024-05-28 DOI: 10.1016/j.engmic.2024.100154

Xin Sun , Haobin Zhang , Yuping Jia , Jingyi Li , Meirong Jia

{"title":"CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids","authors":"Xin Sun , Haobin Zhang , Yuping Jia , Jingyi Li , Meirong Jia","doi":"10.1016/j.engmic.2024.100154","DOIUrl":"https://doi.org/10.1016/j.engmic.2024.100154","url":null,"abstract":"<div><p>Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis of terpenoids in microorganisms offers an alternative and sustainable approach for efficient production. Various genome-editing technologies have been developed for microbial strain construction. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) is the most commonly used system owing to its outstanding efficiency and convenience in genome editing. In this review, the basic principles of CRISPR-Cas9 systems are briefly introduced and their applications in engineering bacteria for the production of plant-derived terpenoids are summarized. The aim of this review is to provide an overview of the current developments of CRISPR-Cas9-based genome-editing technologies in bacterial engineering, concluding with perspectives on the challenges and opportunities of these technologies.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000171/pdfft?md5=2cfc44e8e076429caeecf8ed97dbd95a&pid=1-s2.0-S2667370324000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose 关键氨基酸残基决定了毛霉菌转运体 Xltr1p 对葡萄糖、甘露糖和半乳糖的底物选择性

Engineering Microbiology Pub Date : 2024-05-22 DOI: 10.1016/j.engmic.2024.100151

Wei Ma , Shiyu Yuan , Zixian Wang , Kangle Niu , Fengyi Li , Lulu Liu , Lijuan Han , Xu Fang

{"title":"Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose","authors":"Wei Ma , Shiyu Yuan , Zixian Wang , Kangle Niu , Fengyi Li , Lulu Liu , Lijuan Han , Xu Fang","doi":"10.1016/j.engmic.2024.100151","DOIUrl":"10.1016/j.engmic.2024.100151","url":null,"abstract":"<div><p>This research identified four amino acid residues (Leu174, Asn297, Tyr301, and Gln291) that contribute to substrate recognition by the high-affinity glucose transporter Xltr1p from <em>Trichoderma reesei</em>. Potential hotspots affecting substrate specificity were selected through homology modeling, evolutionary conservation analyses, and substrate-docking modeling of Xltr1p. Variants carrying mutations at these hotspots were subsequently obtained via in silico screening. Replacement of Leu174 or Asn297 in Xltr1p with alanine resulted in loss of hexose transport activity, indicating that Leu174 and Asn297 play essential roles in hexose transport. The Y301W variant exhibited accelerated mannose transport, but lost galactose transport capacity, and mutation of Gln291 to alanine greatly accelerated mannose transport. These results suggest that amino acids located in transmembrane α-helix 7 (Asn297, Tyr301, and Gln291) play critical roles in substrate recognition by the hexose transporter Xltr1p. Our results will help expand the potential applications of this transporter and provide insights into the mechanisms underlying its function and specificity.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000146/pdfft?md5=bd1fae594efb50f04e5007f9cb46944d&pid=1-s2.0-S2667370324000146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141143027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of host proteins that interact with African swine fever virus pE301R 鉴定与非洲猪瘟病毒 pE301R 相互作用的宿主蛋白质

Engineering Microbiology Pub Date : 2024-04-05 DOI: 10.1016/j.engmic.2024.100149

Menghan Shi , Niu Zhou , Mengchen Xiu , Xiangzhi Li , Fen Shan , Wu Chen , Wanping Li , Cheng-Ming Chiang , Xiaodong Wu , Youming Zhang , Aiying Li , Jingjing Cao

{"title":"Identification of host proteins that interact with African swine fever virus pE301R","authors":"Menghan Shi , Niu Zhou , Mengchen Xiu , Xiangzhi Li , Fen Shan , Wu Chen , Wanping Li , Cheng-Ming Chiang , Xiaodong Wu , Youming Zhang , Aiying Li , Jingjing Cao","doi":"10.1016/j.engmic.2024.100149","DOIUrl":"https://doi.org/10.1016/j.engmic.2024.100149","url":null,"abstract":"<div><p>African swine fever virus (ASFV) infection poses enormous threats and challenges to the global pig industry; however, no effective vaccine is available against ASFV, attributing to the huge viral genome (approximately189 kb) and numerous encoding products (>150 genes) due to the limited understanding on the molecular mechanisms of viral pathogenesis. Elucidating the host-factor/viral-protein interaction network will reveal new targets for developing novel antiviral therapies. Using proteomic analysis, we identified 255 cellular proteins that interact with the ASFV-encoded pE301R protein when transiently expressed in HEK293T cells. Gene ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) database enrichment, and protein-protein interaction (PPI) network analyses revealed that pE301R-interacting host proteins are potentially involved in various biological processes, including protein translation and folding, response to stimulation, and mitochondrial transmembrane transport. The interactions of two putative cellular proteins (apoptosis inducing factor mitochondria associated 1 (AIFM1) and vimentin (VIM)) with pE301R-apoptosis inducing factor have been verified by co-immunoprecipitation. Our study revealed the inhibitory role of pE301R in interferon (IFN) induction that involves VIM sequestration by pE301R, identified interactions between ASFV pE301R and cellular proteins, and predicted the potential function of pE301R and its associated biological processes, providing valuable information to enhance our understanding of viral protein function, pathogenesis, and potential candidates for the prevention and control of ASFV infection.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100149"},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000122/pdfft?md5=3dc64fb6bed8b0fa38356bd80c5e7daf&pid=1-s2.0-S2667370324000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-methyltransferase CbzMT catalyzes iterative 3,4-dimethylations for carbazomycin biosynthesis O-甲基转移酶 CbzMT 催化迭代 3,4-二甲基化，促进卡巴霉素的生物合成

Engineering Microbiology Pub Date : 2024-04-02 DOI: 10.1016/j.engmic.2024.100150

Baixin Lin, Dashan Zhang, Junbo Wang, Yongjian Qiao, Jinjin Wang, Zixin Deng, Lingxin Kong, Delin You

{"title":"O-methyltransferase CbzMT catalyzes iterative 3,4-dimethylations for carbazomycin biosynthesis","authors":"Baixin Lin, Dashan Zhang, Junbo Wang, Yongjian Qiao, Jinjin Wang, Zixin Deng, Lingxin Kong, Delin You","doi":"10.1016/j.engmic.2024.100150","DOIUrl":"10.1016/j.engmic.2024.100150","url":null,"abstract":"<div><p>Carbazomycins (<strong>1</strong>–<strong>8</strong>) are a subgroup of carbazole derivatives that contain oxygen at the C3 and C4 positions and an unusual asymmetric substitution pattern. Several of these compounds exhibit antifungal and antioxidant activities. To date, no systematic biosynthetic studies have been conducted on carbazomycins. In this study, carbazomycins A and B (<strong>1</strong> and <strong>2</strong>) were isolated from <em>Streptomyces luteosporeus</em> NRRL 2401 using a one-strain-many-compound (OSMAC)-guided natural product mining screen. A biosynthetic gene cluster (BGC) was identified, and possible biosynthetic pathways for <strong>1</strong> and <strong>2</strong> were proposed. The <em>in vivo</em> genetic manipulation of the O-methyltransferase-encoding gene <em>cbzMT</em> proved indispensable for <strong>1</strong> and <strong>2</strong> biosynthesis. Size exclusion chromatography indicated that CbzMT was active as a dimer. <em>In vitro</em> biochemical assays confirmed that CbzMT could repeatedly act on the hydroxyl groups at C3 and C4, producing monomethylated <strong>2</strong> and dimethylated <strong>1</strong>. Monomethylated carbazomycin B (<strong>2</strong>) is not easily methylated; however, CbzMT seemingly prefers the dimethylation of the dihydroxyl substrate (<strong>12</strong>) to <strong>1</strong>, even with a low conversion efficiency. These findings not only improve the understanding of carbazomycin biosynthesis but also expand the inventory of OMT-catalyzing iterative methylations on different acceptor sites, paving the way for engineering biocatalysts to synthesize new active carbazomycin derivatives.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000134/pdfft?md5=3368a6d51be469c2d456f31ac0ae09eb&pid=1-s2.0-S2667370324000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140776534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0