Engineering Microbiology最新文献

{"title":"A novel strategy to protect prokaryotic cells from virus infection","authors":"Yoshizumi Ishino","doi":"10.1016/j.engmic.2024.100153","DOIUrl":"https://doi.org/10.1016/j.engmic.2024.100153","url":null,"abstract":"<div><p>The recent discovery of the CRISPR-Cas-mediated acquired immunity system highlights the fact that our knowledge of phage/virus defense mechanisms encoded in bacterial and archaeal genomes is far from complete. Indeed, new prokaryotic immune systems are now continually being discovered. A recent report described a novel glycosylase that recognizes α-glycosyl-hydroxymethyl cytosin (α-Glu-hmC), a modified base observed in the T4 phage genome, where it produces an abasic site, thereby inhibiting the phage propagation.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100153"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266737032400016X/pdfft?md5=3e98fd6b6251c7270234b3d731fe4c7a&pid=1-s2.0-S266737032400016X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141263841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids","authors":"Xin Sun ,&nbsp;Haobin Zhang ,&nbsp;Yuping Jia ,&nbsp;Jingyi Li ,&nbsp;Meirong Jia","doi":"10.1016/j.engmic.2024.100154","DOIUrl":"https://doi.org/10.1016/j.engmic.2024.100154","url":null,"abstract":"<div><p>Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis of terpenoids in microorganisms offers an alternative and sustainable approach for efficient production. Various genome-editing technologies have been developed for microbial strain construction. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) is the most commonly used system owing to its outstanding efficiency and convenience in genome editing. In this review, the basic principles of CRISPR-Cas9 systems are briefly introduced and their applications in engineering bacteria for the production of plant-derived terpenoids are summarized. The aim of this review is to provide an overview of the current developments of CRISPR-Cas9-based genome-editing technologies in bacterial engineering, concluding with perspectives on the challenges and opportunities of these technologies.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000171/pdfft?md5=2cfc44e8e076429caeecf8ed97dbd95a&pid=1-s2.0-S2667370324000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose","authors":"Wei Ma ,&nbsp;Shiyu Yuan ,&nbsp;Zixian Wang ,&nbsp;Kangle Niu ,&nbsp;Fengyi Li ,&nbsp;Lulu Liu ,&nbsp;Lijuan Han ,&nbsp;Xu Fang","doi":"10.1016/j.engmic.2024.100151","DOIUrl":"10.1016/j.engmic.2024.100151","url":null,"abstract":"<div><p>This research identified four amino acid residues (Leu174, Asn297, Tyr301, and Gln291) that contribute to substrate recognition by the high-affinity glucose transporter Xltr1p from <em>Trichoderma reesei</em>. Potential hotspots affecting substrate specificity were selected through homology modeling, evolutionary conservation analyses, and substrate-docking modeling of Xltr1p. Variants carrying mutations at these hotspots were subsequently obtained via in silico screening. Replacement of Leu174 or Asn297 in Xltr1p with alanine resulted in loss of hexose transport activity, indicating that Leu174 and Asn297 play essential roles in hexose transport. The Y301W variant exhibited accelerated mannose transport, but lost galactose transport capacity, and mutation of Gln291 to alanine greatly accelerated mannose transport. These results suggest that amino acids located in transmembrane α-helix 7 (Asn297, Tyr301, and Gln291) play critical roles in substrate recognition by the hexose transporter Xltr1p. Our results will help expand the potential applications of this transporter and provide insights into the mechanisms underlying its function and specificity.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000146/pdfft?md5=bd1fae594efb50f04e5007f9cb46944d&pid=1-s2.0-S2667370324000146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141143027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of host proteins that interact with African swine fever virus pE301R","authors":"Menghan Shi ,&nbsp;Niu Zhou ,&nbsp;Mengchen Xiu ,&nbsp;Xiangzhi Li ,&nbsp;Fen Shan ,&nbsp;Wu Chen ,&nbsp;Wanping Li ,&nbsp;Cheng-Ming Chiang ,&nbsp;Xiaodong Wu ,&nbsp;Youming Zhang ,&nbsp;Aiying Li ,&nbsp;Jingjing Cao","doi":"10.1016/j.engmic.2024.100149","DOIUrl":"https://doi.org/10.1016/j.engmic.2024.100149","url":null,"abstract":"<div><p>African swine fever virus (ASFV) infection poses enormous threats and challenges to the global pig industry; however, no effective vaccine is available against ASFV, attributing to the huge viral genome (approximately189 kb) and numerous encoding products (&gt;150 genes) due to the limited understanding on the molecular mechanisms of viral pathogenesis. Elucidating the host-factor/viral-protein interaction network will reveal new targets for developing novel antiviral therapies. Using proteomic analysis, we identified 255 cellular proteins that interact with the ASFV-encoded pE301R protein when transiently expressed in HEK293T cells. Gene ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) database enrichment, and protein-protein interaction (PPI) network analyses revealed that pE301R-interacting host proteins are potentially involved in various biological processes, including protein translation and folding, response to stimulation, and mitochondrial transmembrane transport. The interactions of two putative cellular proteins (apoptosis inducing factor mitochondria associated 1 (AIFM1) and vimentin (VIM)) with pE301R-apoptosis inducing factor have been verified by co-immunoprecipitation. Our study revealed the inhibitory role of pE301R in interferon (IFN) induction that involves VIM sequestration by pE301R, identified interactions between ASFV pE301R and cellular proteins, and predicted the potential function of pE301R and its associated biological processes, providing valuable information to enhance our understanding of viral protein function, pathogenesis, and potential candidates for the prevention and control of ASFV infection.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100149"},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000122/pdfft?md5=3dc64fb6bed8b0fa38356bd80c5e7daf&pid=1-s2.0-S2667370324000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"O-methyltransferase CbzMT catalyzes iterative 3,4-dimethylations for carbazomycin biosynthesis","authors":"Baixin Lin,&nbsp;Dashan Zhang,&nbsp;Junbo Wang,&nbsp;Yongjian Qiao,&nbsp;Jinjin Wang,&nbsp;Zixin Deng,&nbsp;Lingxin Kong,&nbsp;Delin You","doi":"10.1016/j.engmic.2024.100150","DOIUrl":"10.1016/j.engmic.2024.100150","url":null,"abstract":"<div><p>Carbazomycins (<strong>1</strong>–<strong>8</strong>) are a subgroup of carbazole derivatives that contain oxygen at the C3 and C4 positions and an unusual asymmetric substitution pattern. Several of these compounds exhibit antifungal and antioxidant activities. To date, no systematic biosynthetic studies have been conducted on carbazomycins. In this study, carbazomycins A and B (<strong>1</strong> and <strong>2</strong>) were isolated from <em>Streptomyces luteosporeus</em> NRRL 2401 using a one-strain-many-compound (OSMAC)-guided natural product mining screen. A biosynthetic gene cluster (BGC) was identified, and possible biosynthetic pathways for <strong>1</strong> and <strong>2</strong> were proposed. The <em>in vivo</em> genetic manipulation of the O-methyltransferase-encoding gene <em>cbzMT</em> proved indispensable for <strong>1</strong> and <strong>2</strong> biosynthesis. Size exclusion chromatography indicated that CbzMT was active as a dimer. <em>In vitro</em> biochemical assays confirmed that CbzMT could repeatedly act on the hydroxyl groups at C3 and C4, producing monomethylated <strong>2</strong> and dimethylated <strong>1</strong>. Monomethylated carbazomycin B (<strong>2</strong>) is not easily methylated; however, CbzMT seemingly prefers the dimethylation of the dihydroxyl substrate (<strong>12</strong>) to <strong>1</strong>, even with a low conversion efficiency. These findings not only improve the understanding of carbazomycin biosynthesis but also expand the inventory of OMT-catalyzing iterative methylations on different acceptor sites, paving the way for engineering biocatalysts to synthesize new active carbazomycin derivatives.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000134/pdfft?md5=3368a6d51be469c2d456f31ac0ae09eb&pid=1-s2.0-S2667370324000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140776534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electricity generation by Pseudomonas putida B6-2 in microbial fuel cells using carboxylates and carbohydrate as substrates","authors":"Xiaoyan Qi ,&nbsp;Huangwei Cai ,&nbsp;Xiaolei Wang ,&nbsp;Ruijun Liu ,&nbsp;Ting Cai ,&nbsp;Sen Wang ,&nbsp;Xueying Liu ,&nbsp;Xia Wang","doi":"10.1016/j.engmic.2024.100148","DOIUrl":"10.1016/j.engmic.2024.100148","url":null,"abstract":"<div><p>Microbial fuel cells (MFCs) employing <em>Pseudomonas putida</em> B6-2 (ATCC BAA-2545) as an exoelectrogen have been developed to harness energy from various conventional substrates, such as acetate, lactate, glucose, and fructose. Owing to its metabolic versatility, <em>P. putida</em> B6-2 demonstrates adaptable growth rates on diverse, cost-effective carbon sources within MFCs, exhibiting distinct energy production characteristics. Notably, the anode chamber's pH rises with carboxylates' (acetate and lactate) consumption and decreases with carbohydrates' (glucose and fructose) utilization. The MFC utilizing fructose as a substrate achieved the highest power density at 411 mW m⁻². Initial analysis revealed that <em>P. putida</em> B6-2 forms biofilms covered with nanowires, contributing to bioelectricity generation. These microbial nanowires are likely key players in direct extracellular electron transport through physical contact. This study established a robust foundation for producing valuable compounds and bioenergy from common substrates in bioelectrochemical systems (BESs) utilizing <em>P. putida</em> as an exoelectrogen.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100148"},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000110/pdfft?md5=eff030301bd91a1d0c97ae88c88b75b9&pid=1-s2.0-S2667370324000110-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140399510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carotenoid productivity in human intestinal bacteria Eubacterium limosum and Leuconostoc mesenteroides with functional analysis of their carotenoid biosynthesis genes","authors":"Wataru Matsumoto ,&nbsp;Miho Takemura ,&nbsp;Haruka Nanaura ,&nbsp;Yuta Ami ,&nbsp;Takashi Maoka ,&nbsp;Kazutoshi Shindo ,&nbsp;Shin Kurihara ,&nbsp;Norihiko Misawa","doi":"10.1016/j.engmic.2024.100147","DOIUrl":"10.1016/j.engmic.2024.100147","url":null,"abstract":"<div><p>The human intestinal microbiota that comprise over 1,000 species thrive in dark and anaerobic environments. They are recognized for the production of diverse low-molecular-weight metabolites crucial to human health and diseases. Carotenoids, low-molecular-weight pigments known for their antioxidative activity, are delivered to humans through oral intake. However, it remains unclear whether human intestinal bacteria biosynthesize carotenoids as part of the <em>in-situ</em> microbiota. In this study, we investigated carotenoid synthesis genes in various human gut and probiotic bacteria. As a result, novel candidates, the <em>crtM</em> and <em>crtN</em> genes, were identified in the carbon monoxide-utilizing gut anaerobe <em>Eubacterium limosum</em> and the lactic acid bacterium <em>Leuconostoc mesenteroides</em> subsp. <em>mesenteroides</em>. These gene candidates were isolated, introduced into <em>Escherichia coli</em>, which synthesized a carotenoid substrate, and cultured aerobically. Structural analysis of the resulting carotenoids revealed that the <em>crtM</em> and <em>crtN</em> gene candidates of <em>E. limosum</em> and L. <em>mesenteroides</em> mediate the production of 4,4′-diaponeurosporene through 15-<em>cis</em>-4,4′-diapophytoene. Evaluation of the <em>crtE</em>-homologous genes in these bacteria indicated their non-functionality for C₄₀-carotenoid production. <em>E. limosum</em> and L. <em>mesenteroides</em>, along with the known carotenogenic lactic acid bacterium <em>Lactiplantibacillus plantarum</em>, were observed to produce no carotenoids under strictly anaerobic conditions. The two lactic acid bacteria synthesized detectable levels of 4,4′-diaponeurosporene under semi-aerobic conditions. The findings highlight that the obligate anaerobe <em>E. limosum</em> retains aerobically functional C₃₀-carotenoid biosynthesis genes, potentially with no immediate self-utility, suggesting an evolutionary direction in carotenoid biosynthesis. (229 words)</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100147"},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000109/pdfft?md5=a444c059b576cdeadbaf11dfc4968f7c&pid=1-s2.0-S2667370324000109-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140281374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the cathode chamber in microbial electrosynthesis: A comprehensive review of key factors","authors":"Ting Cai ,&nbsp;Xinyu Gao ,&nbsp;Xiaoyan Qi ,&nbsp;Xiaolei Wang ,&nbsp;Ruijun Liu ,&nbsp;Lei Zhang ,&nbsp;Xia Wang","doi":"10.1016/j.engmic.2024.100141","DOIUrl":"10.1016/j.engmic.2024.100141","url":null,"abstract":"<div><p>The consumption of non-renewable fossil fuels has directly contributed to a dramatic rise in global carbon dioxide (CO₂) emissions, posing an ongoing threat to the ecological security of the Earth. Microbial electrosynthesis (MES) is an innovative energy regeneration strategy that offers a gentle and efficient approach to converting CO₂ into high-value products. The cathode chamber is a vital component of an MES system and its internal factors play crucial roles in improving the performance of the MES system. Therefore, this review aimed to provide a detailed analysis of the key factors related to the cathode chamber in the MES system. The topics covered include inward extracellular electron transfer pathways, cathode materials, applied cathode potentials, catholyte pH, and reactor configuration. In addition, this review analyzes and discusses the challenges and promising avenues for improving the conversion of CO₂ into high-value products via MES.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100141"},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000043/pdfft?md5=0045968362299ca70bded635c93f6f6d&pid=1-s2.0-S2667370324000043-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139966064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of homologous recombination/recombineering on human adenovirus genome engineering: Not the only but the most competent solution","authors":"Lisa-Marie Dawson ,&nbsp;Montaha Alshawabkeh ,&nbsp;Katrin Schröer ,&nbsp;Fatima Arakrak,&nbsp;Anja Ehrhardt,&nbsp;Wenli Zhang","doi":"10.1016/j.engmic.2024.100140","DOIUrl":"10.1016/j.engmic.2024.100140","url":null,"abstract":"<div><p>Adenoviruses typically cause mild illnesses, but severe diseases may occur primarily in immunodeficient individuals, particularly children. Recently, adenoviruses have garnered significant interest as a versatile tool in gene therapy, tumor treatment, and vaccine vector development. Over the past two decades, the advent of recombineering, a method based on homologous recombination, has notably enhanced the utility of adenoviral vectors in therapeutic applications. This review summarizes recent advancements in the use of human adenoviral vectors in medicine and discusses the pivotal role of recombineering in the development of these vectors. Additionally, it highlights the current achievements and potential future impact of therapeutic adenoviral vectors.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 1","pages":"Article 100140"},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000031/pdfft?md5=06fac970e5d6d24bc3d15d640a5837dc&pid=1-s2.0-S2667370324000031-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139827339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances on the consolidated bioprocessing of lignocellulosic biomass","authors":"Zhongye Li ,&nbsp;Pankajkumar R. Waghmare ,&nbsp;Lubbert Dijkhuizen ,&nbsp;Xiangfeng Meng ,&nbsp;Weifeng Liu","doi":"10.1016/j.engmic.2024.100139","DOIUrl":"10.1016/j.engmic.2024.100139","url":null,"abstract":"<div><p>Lignocellulosic biomass is an abundant and renewable bioresource for the production of biofuels and biochemical products. The classical biorefinery process for lignocellulosic degradation and conversion comprises three stages, i.e., pretreatment, enzymatic saccharification, and fermentation. However, the complicated pretreatment process, high cost of cellulase production, and insufficient production performance of fermentation strains have restricted the industrialization of biorefinery. Consolidated bioprocessing (CBP) technology combines the process of enzyme production, enzymatic saccharification, and fermentation in a single bioreactor using a specific microorganism or a consortium of microbes and represents another approach worth exploring for the production of chemicals from lignocellulosic biomass. The present review summarizes the progress made in research of CBP technology for lignocellulosic biomass conversion. In this review, different CBP strategies in lignocellulose biorefinery are reviewed, including CBP with natural lignocellulose-degrading microorganisms as the chassis, CBP with biosynthetic microorganisms as the chassis, and CBP with microbial co-culturing systems. This review provides new perspectives and insights on the utilization of low-cost feedstock lignocellulosic biomass for production of biochemicals.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 2","pages":"Article 100139"},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266737032400002X/pdfft?md5=c68277253e9d4a1532c115f4aa451087&pid=1-s2.0-S266737032400002X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139883128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}