Characterization of a small non-coding RNA S612 in Bacillus subtilis

Engineering Microbiology Pub Date : 2024-12-09 DOI:10.1016/j.engmic.2024.100186

Anqi Peng , Weijiao Zhang , Haibo Xiong , Luyao Zhang , Jian Cheng , Yang Wang , Zhen Kang

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Abstract

Small regulatory RNAs (sRNAs) are non-coding RNA molecules that fine-tune various cellular processes and respond to various environmental stimuli. In Bacillus subtilis, the regulatory mechanisms and specific targets of several sRNAs remain largely unknown. In this study, we identified and characterized S612 as a self-terminating sRNA in B. subtilis. The expression of S612 is regulated by external signals, including nutrient availability and salt concentration. Overexpression of S612 induced filamentous cells with extensive cellular elongation and complete inhibition of sporulation, indicating its potential to control cell morphology and spore formation. S612 directly targets and downregulates genes through post-transcriptional base pairing with mRNAs, including ylmD, trpE, ycxC, yycS, rapH, and amyE, some of which are involved in cell membrane integrity, cell wall synthesis, and sporulation initiation. Therefore, we propose that S612 is an important post-transcriptional regulator of cell morphology and sporulation.

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枯草芽孢杆菌非编码小RNA S612的鉴定

小调控RNA （Small regulatory RNA, sRNAs）是一种非编码RNA分子，可以对各种细胞过程进行微调，并对各种环境刺激做出反应。在枯草芽孢杆菌中，几种srna的调控机制和特定靶点在很大程度上仍然未知。在这项研究中，我们鉴定并鉴定了S612是枯草芽孢杆菌中一个自终止的sRNA。S612的表达受外界信号的调控，包括养分有效性和盐浓度。过表达S612诱导的丝状细胞具有广泛的细胞伸长和孢子形成的完全抑制，表明其具有控制细胞形态和孢子形成的潜力。S612通过与mrna的转录后碱基配对直接靶向和下调基因，包括ylmD、trpE、ycxC、yycS、rapH和amyE，其中一些基因参与细胞膜完整性、细胞壁合成和孢子形成起始。因此，我们认为S612是细胞形态和孢子形成的重要转录后调节因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Engineering Microbiology

CiteScore

3.90

自引率

0.00%

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