Engineering Microbiology最新文献

Exploring interspecific interaction variability in microbiota: A review 探索微生物群种间相互作用的变异性：综述

Engineering Microbiology Pub Date : 2024-11-09 DOI: 10.1016/j.engmic.2024.100178

Zhong Yu , Zhihao Gan , Ahmed Tawfik , Fangang Meng

引用次数: 0

Proactive monitoring of changes in the microbial community structure in wastewater treatment bioreactors using phospholipid fatty acid analysis 利用磷脂脂肪酸分析主动监测废水处理生物反应器中微生物群落结构的变化

Engineering Microbiology Pub Date : 2024-11-03 DOI: 10.1016/j.engmic.2024.100177

Lawson Mensah , Elise Cartmell , Mandy Fletton , Mark Scrimshaw , Pablo Campo

{"title":"Proactive monitoring of changes in the microbial community structure in wastewater treatment bioreactors using phospholipid fatty acid analysis","authors":"Lawson Mensah , Elise Cartmell , Mandy Fletton , Mark Scrimshaw , Pablo Campo","doi":"10.1016/j.engmic.2024.100177","DOIUrl":"10.1016/j.engmic.2024.100177","url":null,"abstract":"<div><div>Diverse microbial community structures (MCS) in wastewater treatment plants (WWTPs) are vital for effectively removing nutrients and chemicals from wastewater. However, the regular monitoring of MCS in WWTP bioreactors remains unattractive owing to the skill and cost required for deploying modern microbial molecular techniques in the routine assessment of engineered systems. In contrast, low-resolution methods for assessing broad changes in the MCS, such as phospholipid fatty acid (PLFA) analysis, have been used effectively in soil studies for decades. Despite using PLFA analysis in soil remediation studies to capture the long-term effects of environmental changes on MCS, its application in WWTPs, where the microbial mass is dynamic and operational conditions are more fluid, remains limited. In this study, microbial communities in a controlled pilot plant and 12 full-scale activated sludge plants (ASPs) were surveyed over a two-year period using PLFA analysis. This study revealed that changes in the MCS in wastewater bioreactors could be detected using PLFA analysis. The MCS comprised 59 % Gram-negative and 9 % Gram-positive bacteria, 31 % fungi, and 1 % actinomycetes. The abundances of Gram-negative bacteria and fungi were strongly inversely correlated, with an R<sup>2</sup> = 0.93, while the fatty acids cy17:0 and 16:1ω7c positively correlated (R<sup>2</sup> = 0.869). Variations in temperature, solid retention time, and WWTP configuration significantly influenced the MCS in activated sludge reactors. This study showed that WWTP bioreactors can be routinely monitored using PLFA analysis, and changes in the bioreactor profile that may indicate imminent bioreactor failure can be identified.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100177"},"PeriodicalIF":0.0,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Thermomyces lanuginosus lipase on metal-organic frameworks and investigation of their catalytic properties and stability 在金属有机框架上固定热霉菌脂肪酶并研究其催化特性和稳定性

Engineering Microbiology Pub Date : 2024-10-28 DOI: 10.1016/j.engmic.2024.100176

Zeynab Rangraz , Mostafa M. Amini , Zohreh Habibi

{"title":"Immobilization of Thermomyces lanuginosus lipase on metal-organic frameworks and investigation of their catalytic properties and stability","authors":"Zeynab Rangraz , Mostafa M. Amini , Zohreh Habibi","doi":"10.1016/j.engmic.2024.100176","DOIUrl":"10.1016/j.engmic.2024.100176","url":null,"abstract":"<div><div>Surface adsorption is a convenient and readily available method for immobilizing enzymes on metal-organic frameworks (MOFs). Metal-organic framework-5 (MOF-5), isoreticular metal-organic frameworks-3 (IRMOF-3), and multivariate analysis of MOF-5/IRMOF-3 (MMI) with a half-amino group (-NH<sub>2</sub>) were prepared in this study. <em>Thermomyces lanuginosus</em> lipase (TLL) was chosen as a commercially available enzyme for immobilization on the surfaces of these MOFs. Briefly, 1.5 mg of TLL was added to 10 mg of the MOFs, and after 24 h, 67, 74, and 88% of the TLL was immobilized on MOF-5, IRMOF-3, and MMI, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, scanning electron microscopy, energy-dispersive X-ray analysis, and Brunauer–Emmett–Teller analysis were used to characterize the resulting biocomposites. TLL@MOF-5, TLL@IRMOF-3, and TLL@MMI exhibited activities of 55, 75, and 110 U/mg, respectively. Investigation of the activity and stability of the prepared biocatalysts showed that TLL immobilized on MMI was 2.34-fold more active than free TLL. TLL@MMI exhibited high stability and activity even under harsh conditions. After 24 h of incubation in a mixture of 50% (v/v) MeOH, TLL@MMI retained 80% of its activity, whereas TLL@MOF-5 and free TLL lost 50 and 60% of their activities, respectively. TLL@MMI was used to synthesize 2-arylidenehydrazinyl-4-arylthiozole derivatives (91–98%) in a one-pot vessel by adding benzaldehydes, phenacyl bromides, and thiosemicarbazide to water. The efficiency of the 4a derivative with free TLL was 43%, whereas that with TLL@MMI was 98%.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100176"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The way to uncovering and utilizing marine microbial resources 发掘和利用海洋微生物资源之路

Engineering Microbiology Pub Date : 2024-09-24 DOI: 10.1016/j.engmic.2024.100175

Zhi-Feng Zhang, Meng Li

引用次数: 0

Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing 通过基于嗜热菌的综合生物加工技术利用木质纤维素生产生物燃料

Engineering Microbiology Pub Date : 2024-09-10 DOI: 10.1016/j.engmic.2024.100174

Yilin Le , Mengqi Zhang , Pengju Wu , Huilei Wang , Jinfeng Ni

{"title":"Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing","authors":"Yilin Le , Mengqi Zhang , Pengju Wu , Huilei Wang , Jinfeng Ni","doi":"10.1016/j.engmic.2024.100174","DOIUrl":"10.1016/j.engmic.2024.100174","url":null,"abstract":"<div><div>The depletion of fossil fuels and their impact on the environment have led to efforts to develop alternative sustainable fuels. While biofuel derived from lignocellulose is considered a sustainable, renewable, and green energy source, enhancing biofuel production and achieving a cost-effective bioconversion of lignocellulose at existing bio-refineries remains a challenge. Consolidated bioprocessing (CBP) using thermophiles can simplify this operation by integrating multiple processes, such as hydrolytic enzyme production, lignocellulose degradation, biofuel fermentation, and product distillation. This paper reviews recent developments in the conversion of lignocellulose to biofuel using thermophile-based CBP. First, advances in thermostable enzyme and thermophilic lignocellulolytic microorganism discovery and development for lignocellulosic biorefinery use are outlined. Then, several thermophilic CBP candidates and thermophilic microbes engineered to drive CBP of lignocellulose are reviewed. CRISPR/Cas-based genome editing tools developed for thermophiles are also highlighted. The potential applications of the Design-Build-Test-Learn (DBTL) synthetic biology strategy for designing and constructing thermophilic CBP hosts are also discussed in detail. Overall, this review illustrates how to develop highly sophisticated thermophilic CBP hosts for use in lignocellulosic biorefinery applications.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100174"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Genetically engineered bacteria as inflammatory bowel disease therapeutics 作为炎症性肠病疗法的基因工程细菌

Engineering Microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.engmic.2024.100167

Zhen-Ping Zou , Xiao-Peng Zhang , Qian Zhang, Bin-Cheng Yin, Ying Zhou, Bang-Ce Ye

引用次数: 0

Mechanisms of tigecycline resistance in Gram-negative bacteria: A narrative review 革兰氏阴性细菌对替加环素产生耐药性的机制：叙述性综述

Engineering Microbiology Pub Date : 2024-08-17 DOI: 10.1016/j.engmic.2024.100165

Wenya Su , Wenjia Wang , Ling Li , Mengge Zhang , Hai Xu , Chengzhang Fu , Xiuhua Pang , Mingyu Wang

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Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439 委内瑞拉链霉菌 ATCC 15439 整个 CYPome 和 Fdxome 的功能分析

Engineering Microbiology Pub Date : 2024-08-13 DOI: 10.1016/j.engmic.2024.100166

Shuai Li , Zhong Li , Guoqiang Zhang , Vlada B. Urlacher , Li Ma , Shengying Li

{"title":"Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439","authors":"Shuai Li , Zhong Li , Guoqiang Zhang , Vlada B. Urlacher , Li Ma , Shengying Li","doi":"10.1016/j.engmic.2024.100166","DOIUrl":"10.1016/j.engmic.2024.100166","url":null,"abstract":"<div><p>Cytochrome P450 enzymes (CYPs or P450s) and ferredoxins (Fdxs) are ubiquitously distributed in all domains of life. Bacterial P450s are capable of catalyzing various oxidative reactions with two electrons usually donated by Fdxs. Particularly in <em>Streptomyces</em>, there are abundant P450s that have exhibited outstanding biosynthetic capacity of bioactive metabolites and great potential for xenobiotic metabolisms. However, no systematic study has been conducted on physiological functions of the whole cytochrome P450 complement (CYPome) and ferredoxin complement (Fdxome) of any <em>Streptomyces</em> strain to date<em>,</em> leaving a significant knowledge gap in microbial functional genomics. Herein, we functionally analyze the whole CYPome and Fdxome of <em>Streptomyces venezuelae</em> ATCC 15439 by investigating groups of single and sequential P450 deletion mutants, single P450 overexpression mutants, and Fdx gene deletion or repression mutants. Construction of an unprecedented P450-null mutant strain indicates that none of P450 genes are essential for <em>S. venezuelae</em> in maintaining its survival and normal morphology. The non-housekeeping Fdx1 and housekeeping Fdx3 not only jointly support the cellular activity of the prototypic P450 enzyme PikC, but also play significant regulatory functions. These findings significantly advance the understandings of the native functionality of P450s and Fdxs as well as their cellular interactions.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 4","pages":"Article 100166"},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000286/pdfft?md5=a8270b363730ae79371412a1a3077ca0&pid=1-s2.0-S2667370324000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system 瘤胃分离出的皮氏不动杆菌 ROBY 的纤维素分解特性及潜在控释给药系统的设计

Engineering Microbiology Pub Date : 2024-08-03 DOI: 10.1016/j.engmic.2024.100164

Ruken Sariboga , Omer Faruk Sarioglu

{"title":"Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system","authors":"Ruken Sariboga , Omer Faruk Sarioglu","doi":"10.1016/j.engmic.2024.100164","DOIUrl":"10.1016/j.engmic.2024.100164","url":null,"abstract":"<div><p>A novel cellulolytic bacterial strain, ROBY, was isolated from a bovine rumen sample using the enrichment culture method. This isolate was found to be <em>Acinetobacter pittii</em>, with >99 % similarity according to 16S rRNA gene sequence analysis. The potential use of this strain in combination with doxorubicin (Dox)-integrated cellulose nanoparticles (Dox-CNPs) was evaluated as a proof-of-concept study for the further development of this approach as a novel controlled-release drug delivery strategy. The isolate can utilize CNPs as the sole carbon source for growth and degrade both Dox-CNPs and empty CNPs with high efficiency. Extracellular cellulases isolated from bacteria may also be used to trigger Dox release. The results also demonstrated that the release of Dox into the environment due to nanoparticle degradation in the samples incubated with Dox-CNPs significantly affected bacterial cell viability (∼75 % decrease), proving the release of Dox due to bacterial cellulase activity and suggesting the great potential of this approach for further development.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100164"},"PeriodicalIF":0.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000262/pdfft?md5=154741f24ca8241e1873123c2ea33593&pid=1-s2.0-S2667370324000262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications Paenibacillus elgii 上清液对筛选具有生物技术应用潜力的细菌菌株的影响

Engineering Microbiology Pub Date : 2024-07-22 DOI: 10.1016/j.engmic.2024.100163

I. C． Cunha-Ferreira , C. S． Vizzotto , T. D． Frederico , J． Peixoto , L. S Carvalho , M. R． Tótola , R. H． Krüger

{"title":"Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications","authors":"I. C． Cunha-Ferreira , C. S． Vizzotto , T. D． Frederico , J． Peixoto , L. S Carvalho , M. R． Tótola , R. H． Krüger","doi":"10.1016/j.engmic.2024.100163","DOIUrl":"10.1016/j.engmic.2024.100163","url":null,"abstract":"<div><p>The biotechnological industry faces a crucial demand for novel bioactive compounds, particularly antimicrobial agents, to address the rising challenge of bacterial resistance to current available antibiotics. Traditional strategies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial producers. This study presents a protocol for targeted selection of bacterial strains using the supernatant of <em>Paenibacillus elgii</em>, which produces abundant signal molecules and antimicrobial peptides. Soil samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant, indicating their potential to produce similar compounds. The bacterial strains isolated through this method were assessed for their antibacterial activity. In addition, the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological interest. This study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"4 3","pages":"Article 100163"},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000250/pdfft?md5=3320eb58e754f7bdc2a2f5525731fd2b&pid=1-s2.0-S2667370324000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0