Engineering Microbiology最新文献

{"title":"Identification and functional characterization of a potential l-Homoserine exporter in Corynebacterium glutamicum","authors":"Xiaodi Liu ,&nbsp;Xiangyu Zhu ,&nbsp;Wenxin Jiang ,&nbsp;Huanmin Du","doi":"10.1016/j.engmic.2025.100240","DOIUrl":"10.1016/j.engmic.2025.100240","url":null,"abstract":"<div><div>Exporter protein systems play a crucial role in the efficient production of valuable chemicals. However, the lack of active exporters significantly limits the application of industrial bio-based production, making the identification and utilization of novel exporters highly important. In this study, we discovered a novel <span>l</span>-Homoserine exporter, Cg0701, in <em>Corynebacterium glutamicum</em> through homology analysis. First, tolerance assays revealed that the <em>cg0701</em> overexpression strain (CgH-2) exhibited a 10.45% increase in cell growth compared to the control when cultivated with 30 g/L-Homoserine. Additionally, export assays demonstrated that the <span>l</span>-Homoserine export capacity of CgH-2 increased by approximately 30%. Furthermore, genomic overexpression of <em>cg0701</em> in an <span>l</span>-Homoserine-producing chassis also enhanced both tolerance and export activity. As a result, the recombinant strain CgH-11 produced 10.79 g/L-Homoserine in shake flask cultures and 48.72 g/L in a 5 L fermenter, representing improvements of 19.89% and 24.44%, respectively. In summary, our results indicate that Cg0701 is a novel <span>l</span>-Homoserine exporter in <em>C. glutamicum</em>, enriching our understanding of amino acid export systems and providing a valuable target for the construction of <span>l</span>-Homoserine microbial cell factories.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145121025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial co-cultivation for the degradation of polystyrene plastics","authors":"Yingbo Yuan ,&nbsp;Tianyuan Su ,&nbsp;Yi Zheng,&nbsp;Baoyue Liu,&nbsp;Yuanfei Han,&nbsp;Zhongcan Wang,&nbsp;Quanfeng Liang,&nbsp;Longyang Dian,&nbsp;Qingsheng Qi","doi":"10.1016/j.engmic.2025.100232","DOIUrl":"10.1016/j.engmic.2025.100232","url":null,"abstract":"<div><div>Polystyrene (PS) is a polyolefin plastic that is used extensively in food packaging. The chemical structure of PS is extremely stable owing to its C-C backbone and styrene rings, making it highly resistant to biodegradation, which causes serious environmental pollution and health threats. Although certain microorganisms have been reported to degrade PS waste, most studies have focused on the changes in the molecular weight and surface structure of plastics. These slight degradation phenomena make it extremely difficult to detect the degradation products, thus challenging the definitive demonstration of PS degradation. This study investigated the co-cultivation of the polyolefin plastic-degrading bacterium <em>Raoultella</em> sp. DY2415 and the benzoic acid bioconversion strain <em>Pseudomonas putida</em> KT2440-ΔRBC. BA is a possible degradation product of PS and can be converted by <em>P. putida</em> KT2440-ΔRBC into the high value-added compound muconic acid (MA). After co-cultivation, MA was detected in the medium, indicating that <em>Raoultella</em> sp. DY2415 degraded PS and generated BA, which was subsequently utilized by <em>P. putida</em> KT2440-ΔRBC for MA synthesis. This study demonstrated the biodegradation of PS and the synthesis of MA through a fully biological process, thereby promoting the circular economy of plastics.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100232"},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel therapeutic strategy of methicillin-resistant Staphylococcus aureus","authors":"Ying Wang ,&nbsp;Mengyan Xu ,&nbsp;Hanne Ingmer","doi":"10.1016/j.engmic.2025.100231","DOIUrl":"10.1016/j.engmic.2025.100231","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> is a major public health threat, largely due to its remarkable capacity to develop antimicrobial resistance. Zhang <em>et al.</em> recently demonstrated a highly innovative approach to eradicate chronic methicillin-resistant <em>S. aureus</em> infections by inducing bacterial calcification with antibody-polysialic acid conjugates targeting wall teichoic acids, while simultaneously modulating host immune responses via enhanced calprotectin expression and macrophage activation. Despite limitations, this strategy represents a promising and unconventional therapy to combat resistant <em>S. aureus</em> infections.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100231"},"PeriodicalIF":0.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoupling growth phase dependency and metal ion inhibition: A dual engineering strategy for the high-yield biosynthesis of microcin J25 in Escherichia coli","authors":"Guangxin Yang ,&nbsp;Xinchan Wang ,&nbsp;Yunting Zhou ,&nbsp;Xiuliang Ding ,&nbsp;Jinxiu Huang ,&nbsp;Shiyan Qiao ,&nbsp;Aihua Deng ,&nbsp;Haitao Yu","doi":"10.1016/j.engmic.2025.100230","DOIUrl":"10.1016/j.engmic.2025.100230","url":null,"abstract":"<div><div>Microcin J25 (MccJ25) has received substantial attention as a potential solution to the global threat of infection caused by antibiotic-resistant bacteria. However, the industrial fermentation of MccJ25 faces production bottlenecks. It is imperative to further explore the production optimization strategies for MccJ25 to formulate comprehensive approaches for its industrial-scale production and other downstream applications. Here, Fe²⁺ in tap water was identified as a critical inhibitor of MccJ25 biosynthesis, selectively repressing <em>mcjA</em> transcription, which was reversible via 2,2′-bipyridine-mediated chelation. To decouple production from growth phase dependency and Fe²⁺ interference, we engineered <em>Escherichia coli</em> BL21 cells by performing two genetic modifications. First, we replaced the native <em>mcjA</em> promoter with a constitutive promoter (P_Q) to allow its mid-log phase expression. Second, we replaced the native <em>mcjBCD</em> promoter with a medium-strength variant (P₂₂₂₃) that delayed production kinetics without affecting final yields. However, the genomic integration of <em>mcjD</em> alleviated plasmid-borne toxicity, increasing the expression timing and doubling the yield to 240 mg/L. Finally, we computationally optimized the <em>mcjA</em> ribosome-binding site (RBS) to enhance translation efficiency. RBS optimization revealed that a moderate translation initiation efficiency (550,584 arbitrary units [au]) maximized production, whereas excessive efficiency (2,019,712 au) impaired growth and output. These interventions synergistically increased the MccJ25 titer 10-fold, reaching 430 mg/L in batch culture. Our findings establish a robust platform for MccJ25 overproduction, highlighting promoter engineering and translational tuning as pivotal strategies for antimicrobial peptide biosynthesis. This study provides insights for overcoming metabolic constraints in microbial fermentation, advancing the development of peptide-based therapeutics against multidrug-resistant pathogens.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100230"},"PeriodicalIF":0.0,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145121026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous co-cultivation of the thermoacidophilic methanotroph, Methylacidiphilum sp. RTK17.1, and the microalga, Galdieria sp. RTK37.1, for single cell protein production","authors":"Carlos Cartin-Caballero ,&nbsp;Christophe Collet ,&nbsp;Daniel Gapes ,&nbsp;Peter A. Gostomski ,&nbsp;Matthew B. Stott ,&nbsp;Carlo R. Carere","doi":"10.1016/j.engmic.2025.100229","DOIUrl":"10.1016/j.engmic.2025.100229","url":null,"abstract":"<div><div>The verrucomicrobial methanotroph, <em>Methylacidiphilum</em> sp. RTK17.1, and the microalgae, <em>Galdieria</em> sp. RTK37.1 are both thermoacidophilic microorganisms isolated from geothermally heated soils at Rotokawa, Aotearoa-New Zealand. In this work, we used cocultures of <em>Methylacidiphilum</em> sp. RTK17.1 and <em>Galdieria</em> sp. RTK37.1 in batch and continuous systems (45 °C, pH 2.5) to assess their biomass productivity and performance; with the goal of removing methane and carbon dioxide from simulated waste gas streams and assessing the resultant biomass for its potential use as single cell protein. Coculture performance was compared to corresponding axenic cultures and the nutritional suitability of resultant biomass was assessed as a single cell protein feedstock. Stable coculture was achieved in both batch and chemostat systems. In batch experiments, <em>Galdieria</em> sp. RTK37.1 significantly enhanced growth (29 %) and methane oxidation (300 %) rates of <em>Methylacidiphilum</em> sp. RTK17.1, and complete methane removal was achieved without formation of an explosive gas mixture. In steady state chemostat coculture experiments, <em>Galdieria</em> sp. RTK37.1 decreased net volumetric oxygen consumption by 46 %, but its oxygenic activity was unable to supply <em>Methylacidiphilum</em> sp. RTK17.1 with the O₂ required for complete CH₄ removal. Nevertheless, <em>Methylacidiphilum</em> sp. RTK17.1 benefited from the presence of <em>Galdieria</em> sp. RTK37.1 in a low O₂ environment; with O₂ algae-methanotroph cross-feeding playing a fundamental role on their interactions. <em>Methylacidiphilum</em> sp. RTK17.1, <em>Galdieria</em> sp. RTK37.1, and their coculture each displayed similar nutritional profiles, with protein quality comparable to soybean meal and fishmeal feeds used for animals. The biomass needed to meet the daily indispensable amino acid requirements of a 62 kg adult human was 568 g for <em>Methylacidiphilum</em> sp. RTK17.1, 804 g <em>Galdieria</em> sp. RTK37.1, and 754 g for the coculture, with histidine being the limiting amino acid. These thermoacidophilic cocultures, which have not previously been investigated, offer great potential to convert low (or negative) value industrial gas streams into valuable products (e.g. supplementary biofeedstocks).</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100229"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic and molecular characterization of a moderately thermophilic cyanobacterium, Gloeocapsa sp. strain BRSZ","authors":"Sasiprapa Samsri ,&nbsp;Tanwalee Deprom ,&nbsp;Chananwat Kortheerakul ,&nbsp;Sophon Sirisattha ,&nbsp;Stephen B. Pointing ,&nbsp;Hakuto Kageyama ,&nbsp;Rungaroon Waditee-Sirisattha","doi":"10.1016/j.engmic.2025.100226","DOIUrl":"10.1016/j.engmic.2025.100226","url":null,"abstract":"<div><div>A unicellular-colonial cyanobacterium, designated “BRSZ,” was isolated from a neutral-alkaline hot spring in Thailand. Morphological characterization revealed distinctive features consistent with those of the genus <em>Gloeocapsa</em>. Physiological assessments demonstrated that BRSZ is a moderately thermophilic and halotolerant cyanobacterium with the potential for chemoheterotrophic growth in dark conditions. Molecular phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences placed BRSZ within a well-defined <em>Gloeocapsa</em> clade, a finding corroborated by 16S–23S internal transcribed spacer (ITS) rRNA secondary structure analyses. Genome comparisons, including average nucleotide identity (ANI), genome-to-genome distance (GGD), and digital DNA-DNA hybridization (dDDH), between strain BRSZ and closely related taxa showed an ANI value of 95.45 %, near the lower boundary of the species delineation threshold (95–96 %). A GGD of 0.0374 (&gt;0.0258) and dDDH of 69 % (&lt;70 %) further supported genomic differentiation. Genome-based analysis revealed a mycosporine-like amino acid biosynthetic gene cluster likely involved in sunscreen compound production. Cultivation-based production of a UV-absorbing compound confirmed the functional relevance of this gene cluster. These findings expand the described diversity within the <em>Gloeocapsa</em> complex and enhance our understanding of the taxonomy of this group. In addition, they underscored the importance of hot spring environments as sources of novel extremophiles.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100226"},"PeriodicalIF":0.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifidobacterium animalis subsp. lactis genome resources and metabolite profiling at the strain level and their ability to alleviate anxiety-like behavior in a sleep-deprived mouse model","authors":"Yao-Kun Zhang ,&nbsp;Liang Zhang ,&nbsp;Xue Ni ,&nbsp;Shu-Wen Zhang ,&nbsp;Min-Zhi Jiang ,&nbsp;Si-Lu Zhang ,&nbsp;Guo-Xun Xiao ,&nbsp;He Jiang ,&nbsp;Ming-Xia Bi ,&nbsp;Yu-Lin Wang ,&nbsp;Chang Liu ,&nbsp;Shuang-Jiang Liu","doi":"10.1016/j.engmic.2025.100228","DOIUrl":"10.1016/j.engmic.2025.100228","url":null,"abstract":"<div><div><em>Bifidobacterium animalis</em> subsp. <em>lactis</em> is a well-known probiotic with potential benefits for alleviating sub-health symptoms, including immune dysfunction and anxiety. Given the strain-specific nature of its probiotic effects, identifying effective strains for sub-health alleviation is crucial. In this study, we characterized 16 <em>B animalis</em> subsp. <em>lactis</em> isolates from fecal samples and probiotic sources. We assessed the genotype-phenotype correlations related to growth, carbon source utilization, and stress tolerance <em>in vitro</em>. Subsequently, we profiled 107 metabolites (including 28 alcohols and 17 esters) and quantified the levels of short-chain fatty acids and three other organic acids. Three <em>B. animalis</em> strains, GOLDGUT-BB21, WLBA7, and WLBA6, were selected and evaluated in a sleep-deprived mouse model. <em>In vivo</em>, WLBA3 reduced inflammation and oxidative stress by inhibiting the NLRP3 inflammasome pathway and modulating gut microbiota (e.g., <em>Lactobacillus</em> and <em>Alistipes</em>), which in turn significantly improved weight gain and fatigue resistance, attenuated cognitive function, and anxiety-like behavior. These findings provide insights into the diversity of <em>B. animalis</em> subsp. <em>lactis</em> strain resources and highlight the potential of WLBA3 as a candidate for alleviating sub-health symptoms.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100228"},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of cycloastragenol in metabolically engineered yeast","authors":"Jingxian Zhang ,&nbsp;Peng Xu ,&nbsp;Yongjun Wei","doi":"10.1016/j.engmic.2025.100227","DOIUrl":"10.1016/j.engmic.2025.100227","url":null,"abstract":"<div><div>Cycloastragenol is a bioactive, high-value triterpenoid derived from <em>Astragalus membranaceus</em>. Conventional plant-based extraction and chemical synthesis methods are expensive. To our knowledge, this is the first report on the <em>de novo</em> biosynthesis of cycloastragenol in yeast. The mevalonate pathway was reconstituted in yeast peroxisomes, and the engineered yeast produced 656.55 mg/L squalene. Further introduction of heterologous enzymes led the engineered yeast to produce 1.04 mg/L cycloastragenol, which demonstrated the yeast production of value-added medicinal molecules.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100227"},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144831441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of molecular elements and improvement of heat resistance in a thermophilic bacterium","authors":"Jie Cui ,&nbsp;Caifeng Li ,&nbsp;Gongze Cao ,&nbsp;Yuxia Wu ,&nbsp;Shouying Xu ,&nbsp;Youming Zhang ,&nbsp;Xiaoying Bian ,&nbsp;Qiang Tu ,&nbsp;Wentao Zheng","doi":"10.1016/j.engmic.2025.100225","DOIUrl":"10.1016/j.engmic.2025.100225","url":null,"abstract":"<div><div>Engineering microorganisms to withstand extreme temperatures (&gt;80 °C) remains a critical challenge in industrial biotechnology owing to limited genetic tools and poor mechanistic understanding of microbial thermoadaptation. We aimed to develop a novel <em>Geobacillus stearothermophilus</em> strain with remarkable thermal resilience through an integrated approach combining adaptive laboratory evolution and rational genetic engineering. Progressive thermal adaptation (70–80 °C) followed by genome reduction generated a mutant (SL-1–80) with enhanced stability at 80 °C. Subsequent combinatorial overexpression of eight heat-associated genes (<em>murD, cysM, grpE, groES, hsp33, hslO, hrcA, clpE</em>) synergistically extended its survival to 85 °C. Genomic and transcriptomic analyses revealed a triple mechanism: (1) strategic deletion of transposable elements (IS5377/IS4/IS110) reduced genomic instability, (2) co-activation of chaperone systems (GroES-GrpE) and redox homeostasis enzymes (HslO<img>Hsp33) enhanced protein folding and oxidative stress resistance, and (3) metabolic plasticity (BglG and HTH-domain transcriptional repressor), motility optimization (FliY), and transcriptional reprogramming (Sigma-D, DUF47-family chaperone and HTH-domain transcriptional repressor) facilitated nutrient acquisition and motility-based environmental navigation under stress. Furthermore, we established the first high-efficiency electroporation protocol (10⁴ transformants/µg DNA) for this genus, enabling ATP-enhanced heterologous protein expression under heat stress. This study provided a robust platform organism for high-temperature bioprocessing and a mechanistic blueprint for engineering microbial thermotolerance, addressing key limitations in applications such as microbial-enhanced oil recovery and industrial enzyme production.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100225"},"PeriodicalIF":0.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity analysis of phytase-producing bacteria from coastal seawater and sediment and characterization of their phytases","authors":"Xiao-Jie Yuan ,&nbsp;Rui Liu ,&nbsp;Jian Li ,&nbsp;Wen-Xiao Zhao ,&nbsp;Hui-Hui Fu ,&nbsp;Yan-Rong Zhou ,&nbsp;Mei-Ling Sun ,&nbsp;Xiu-Lan Chen ,&nbsp;Yu-Qiang Zhang","doi":"10.1016/j.engmic.2025.100223","DOIUrl":"10.1016/j.engmic.2025.100223","url":null,"abstract":"<div><div>Phytic acid, also known as inositol hexaphosphate (IP6), is one of the most abundant organophosphorus compounds in nature. Its degradation by phytase plays a key role in the natural phosphorus cycle. In addition, phytases are widely used in livestock and poultry feed to enhance phosphorus utilization. While most reported and commercial phytases are derived from terrestrial organisms, relatively few originate from marine microorganisms, and information on the diversity of phytase-producing marine bacteria remains limited. In this study, following enrichment with sodium phytate, we analyzed the bacterial diversity in seawater and sediment samples collected from the coast of Aoshan Bay in Qingdao, China, using 16S rRNA gene amplicon sequencing. A total of 138 OTUs representing 10 phyla, 15 classes, 37 orders, 55 families, and 70 genera were identified. Furthermore, 27 phytase-producing bacterial strains were isolated from the enrichment cultures, primarily belonging to the phyla Firmicutes (14/27) and Proteobacteria (12/27). Five extracellular phytase genes were identified through genome sequencing of three representative strains. These phytases were subsequently expressed and characterized. All were classified as histidine acid phosphatase-type phytases, exhibiting optimal activity at temperatures of 50–60 °C and pH values of 4.0–5.0. Notably, phytase 3919 showed a specific activity as high as 2485.25 U/mg, indicating strong potential for practical applications. This study provides insight into the diversity of coastal bacteria involved in phytic acid degradation, contributing to our understanding of bacterial-driven phosphorus cycling in coastal ecosystems and facilitating the discovery of phytases with industrial potential.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100223"},"PeriodicalIF":0.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}