Engineering Microbiology最新文献

Pathway reprogramming and catalytic network engineering for the production of bioactive aspertetranones from deep-sea Aspergillus versicolor ADS-F20 深海杂色曲霉ADS-F20生产生物活性阿斯特戊酮的途径重编程和催化网络工程

Engineering Microbiology Pub Date : 2026-06-01 Epub Date: 2026-01-02 DOI: 10.1016/j.engmic.2025.100259

Peiyuan Feng , Moli Sang , Wei Zhang

{"title":"Pathway reprogramming and catalytic network engineering for the production of bioactive aspertetranones from deep-sea Aspergillus versicolor ADS-F20","authors":"Peiyuan Feng , Moli Sang , Wei Zhang","doi":"10.1016/j.engmic.2025.100259","DOIUrl":"10.1016/j.engmic.2025.100259","url":null,"abstract":"<div><div>Aspertetranones are a unique class of marine fungal meroditerpenoids characterized by a highly oxygenated, linear 6/6/6/6 tetracyclic core fused to an α-pyrone scaffold. Although the pathway of aspertetranone biosynthesis in <em>Aspergillus ochraceopetaliformis</em> has been partially elucidated, the full potential of these compounds remains untapped. The structural diversity and enzyme promiscuity of tailoring reactions offer unexplored opportunities for the generation of bioactive derivatives through combinatorial biosynthesis. In this study, we identified the <em>atn</em> biosynthetic gene cluster responsible for aspertetranone production in deep-sea-derived <em>Aspergillus versicolor</em> ADS-F20. Through the systematic heterologous expression of 12 key genes in <em>Aspergillus oryzae</em>, the full pathway reconstitution and targeted biosynthesis of 17 metabolites were achieved, thus expanding the known chemical space of meroterpenoids. Notably, bioactivity screening identified compound <strong>6</strong> as having potent antibacterial and antifungal activities against <em>Vibrio vulnificus</em> ATCC 27562 (MIC = 4.50 μg/mL) and <em>Phytophthora nicotianae</em> (MIC = 9.01 μg/mL). Compound <strong>11</strong> demonstrated broad-spectrum anticancer and cytotoxic effects against the K-562, MCF7, and PATU8988T cell lines. This study underscores the power of pathway reprogramming and catalytic network engineering as versatile strategies for expanding the structural and functional diversity of biosynthetic pathway components.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 2","pages":"Article 100259"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a CRISPRi system in Fusarium fujikuroi and its application in gibberellic acid production 藤黑镰刀菌CRISPRi系统的建立及其在赤霉素生产中的应用

Engineering Microbiology Pub Date : 2026-06-01 Epub Date: 2026-01-21 DOI: 10.1016/j.engmic.2026.100260

Yuke Cen , Hang Xiao , Jingwen Jia , Jiajia Mou , Haoyang Li , Jialiang Wang , Yaling Yi , Minghan Li , Zhiqiang Liu , Yuguo Zheng

{"title":"Development of a CRISPRi system in Fusarium fujikuroi and its application in gibberellic acid production","authors":"Yuke Cen , Hang Xiao , Jingwen Jia , Jiajia Mou , Haoyang Li , Jialiang Wang , Yaling Yi , Minghan Li , Zhiqiang Liu , Yuguo Zheng","doi":"10.1016/j.engmic.2026.100260","DOIUrl":"10.1016/j.engmic.2026.100260","url":null,"abstract":"<div><div>Gene knockdown is a pivotal genetic manipulation technique, particularly when targeting lethal genes or genes involved in product synthesis pathways, where complete gene knockout is not a viable option. This approach is particularly valuable in multinucleate species, such as <em>Fusarium fujikuroi</em>, where generating homogeneous gene knockouts is notoriously difficult. To address these limitations, we first screened a set of repression domains, and then leveraged the optimal candidates to construct a CRISPR/dCas9-mediated knockdown platform for <em>F. fujikuroi</em>. By targeting <em>erg9</em>, which encodes squalene synthase, the first committed enzyme in the mevalonate pathway for ergosterol biosynthesis, we successfully diverted a portion of the metabolic flux from sterol production to gibberellic acid (GA) biosynthesis. This strategy minimizes carbon loss to competing pathways while retaining phenotypically normal growth. Additionally, CRISPR/dCas9-mediated knockdown of the dehydrogenase gene <em>des</em> enhanced GA<sub>4</sub> production by 2.62-fold and eliminated the intermediate GA<sub>7</sub>, generating a GA<sub>3+4</sub>-producing strain and fine-tuning its metabolic profile. Using our CRISPRi system, we achieved a 70–89 % reduction in <em>erg9</em> mRNA levels and a 67– 84 % reduction in <em>des</em> mRNA levels. Our findings establish a tailored CRISPRi platform for effective gene repression in <em>F. fujikuroi</em>.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 2","pages":"Article 100260"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of heat resistance of Bacillus thermoamylovorans drives enhanced PET degradation 热淀粉样芽孢杆菌耐热性的增强促进了PET的降解

Engineering Microbiology Pub Date : 2026-06-01 Epub Date: 2025-12-23 DOI: 10.1016/j.engmic.2025.100256

Yi-Mei Cai, Kang-Qi Hu, Xin-Yu Zhang, Hui Liu, Yan Huang, Wei Xia, Jing Wu, Zheng-Fei Yan

{"title":"Enhancement of heat resistance of Bacillus thermoamylovorans drives enhanced PET degradation","authors":"Yi-Mei Cai, Kang-Qi Hu, Xin-Yu Zhang, Hui Liu, Yan Huang, Wei Xia, Jing Wu, Zheng-Fei Yan","doi":"10.1016/j.engmic.2025.100256","DOIUrl":"10.1016/j.engmic.2025.100256","url":null,"abstract":"<div><div>Microbial polyethylene terephthalate (PET) degradation has emerged as an environmentally friendly approach to reducing plastic pollution, but microbial activity is often limited by the high temperatures (60–70 °C) required for efficient degradation. In this study, six endogenous thermotolerant genes were identified in the PET-degrading strain <em>Bacillus thermoamylovorans</em> JQ3. Overexpression of the genes <em>hrcA, hsp20A,</em> and <em>hsp20B</em> significantly enhanced thermotolerance in <em>Escherichia coli</em>, which increased cell viability by >24.2% at temperatures above 40 °C. Similarly, overexpression of <em>hsp20A</em> in <em>B. thermoamylovorans</em> (<em>B. th</em>_Hsp20A) improved its thermotolerance, increasing cell viability by 46.8% at 50 °C and 69.8% at 60 °C. The <em>hsp20A</em>-encoded protein was identified as a small heat shock protein (sHSP). <em>B. th</em>_Hsp20A exhibited significantly improved PET degradation at 60 °C compared with 50 °C, which released 282 µg of terephthalic acid after 7 days of incubation, representing an 84.3% increase compared with the wildtype strain (153 µg). These results highlight that the overexpression of thermotolerant proteins, particularly sHSPs, significantly enhances microbial PET degradation under high-temperature conditions, offering a novel pathway for enhancing microbial PET waste degradation.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 2","pages":"Article 100256"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering host‑defense peptides enhanced by artificial intelligence and nano delivery systems to overcome biofilms and antimicrobial resistance 通过人工智能和纳米递送系统增强的工程宿主防御肽，以克服生物膜和抗菌素耐药性。

Engineering Microbiology Pub Date : 2026-06-01 Epub Date: 2026-05-15 DOI: 10.1016/j.engmic.2026.100277

Raman Krishnamoorthi , Muthuramalingam Kaviyadharshini , Pambayan Ulagan Mahalingam , Moovendran Srinivash , Pitchaimuthu Rajkannan , Mohan Keerthivsan , Paulraj Suganya , Arokia Vijaya Anand Mariadoss

{"title":"Engineering host‑defense peptides enhanced by artificial intelligence and nano delivery systems to overcome biofilms and antimicrobial resistance","authors":"Raman Krishnamoorthi , Muthuramalingam Kaviyadharshini , Pambayan Ulagan Mahalingam , Moovendran Srinivash , Pitchaimuthu Rajkannan , Mohan Keerthivsan , Paulraj Suganya , Arokia Vijaya Anand Mariadoss","doi":"10.1016/j.engmic.2026.100277","DOIUrl":"10.1016/j.engmic.2026.100277","url":null,"abstract":"<div><div>Infections caused by bacteria pose a risk to humanity as drugs become increasingly ineffective as resistance to bacterial strains emerge along with biofilm and persister formation. This review critically evaluates host defense peptides, rational design strategies that have guided next-generation antimicrobial peptide (AMP) discovery, and their current limitations. We also highlight optimization approaches including sequence engineering and chemical modification, synergistic combinations of antibiotics or adjuvants, and nanoscale delivery platforms that enhance stability, targeted delivery, and biofilm penetration. We also discuss the key chemical properties, delivery kinetics, and stimuli-responsive drug delivery for antibacterial and antibiofilm actions as well as the toxic effects of organic- and inorganic-based AMP delivery platforms. This underlines the importance of diverse modification techniques and artificial intelligence (AI)-assisted designs to improve the antibacterial activity, stability, and biocompatibility of AMPs. This study examines the latest advances in the combination of AMPs with drug delivery systems to improve clinical outcomes. Finally, the review discusses the clinical status, research gaps, current obstacles, and prospects of AMPs in antimicrobial resistance (AMR) therapy, offering key findings for the development of innovative AMPs with significant antibacterial activity, stability, and safety for AMR treatment.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 2","pages":"Article 100277"},"PeriodicalIF":0.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148166422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Hachiman defence by a type II toxin-antitoxin system via balancing trade-off between the fitness cost and antiphage activity II型毒素-抗毒素系统通过适应成本和抗噬菌体活性之间的平衡权衡来调节Hachiman防御

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-19 DOI: 10.1016/j.engmic.2025.100254

Xuhui Tian, Ruyi Zheng, Xin Li, Suping Jiang, Fang Wang, Yulong Shen, Qunxin She

{"title":"Modulation of Hachiman defence by a type II toxin-antitoxin system via balancing trade-off between the fitness cost and antiphage activity","authors":"Xuhui Tian, Ruyi Zheng, Xin Li, Suping Jiang, Fang Wang, Yulong Shen, Qunxin She","doi":"10.1016/j.engmic.2025.100254","DOIUrl":"10.1016/j.engmic.2025.100254","url":null,"abstract":"<div><div>Hachiman systems provide innate antiphage immunity across prokaryotic domains. The system encodes a HamA nuclease and a HamB helicase both of which exhibit great diversity in sequence. Phylogenetic analyses of HamA and HamB proteins revealed similar phylogenetic trees for both proteins, falling into three major types. Close examination of one of the subclades identified a distinct subfamily in which most of these Hachiman systems stands alone, however, Hachiman in the <em>Streptococcus</em> genus is combined with PezAT, a distinct pneumococcal epsilon zeta toxin-antitoxin system, yielding the Pez-Ham system. Investigation of a <em>S. thermophilus</em> Pez-Ham system revealed that only the Hachiman system is required for mediating antiphage defence. Biochemical characterization of encoded proteins, i.e., HamA or HamB individually or in protein complex revealed that the HamA nuclease is inactive alone, but upon the formation of heterologous dimer with HamB, the resulting protein complex effectively cleaves DNAs of various forms with a broad specificity (5′-CNNNG-3′), and the nuclease activity is greatly facilitated by ATP-binding in HamB and to a less degree by ATP hydrolysis. Genetic investigations further showed, while the Pez system did not function in antiphage immunity in <em>Escherichia coli</em>, the system repressed the expression of Hachiman, and thereby balancing the trade-off between the fitness cost and the effectiveness of antiphage defence.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100254"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145928177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in polyethylene biodegradation and bioconversion: Microbial, enzymatic, and biotechnological insights 聚乙烯生物降解和生物转化的进展：微生物、酶和生物技术的见解

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-20 DOI: 10.1016/j.engmic.2025.100255

Jie Qiao , Luxuan Wu , Xiaoru Ma , Anni Li , Yannan Tian , Hailong Lin , Dongsheng Guo , Xiujuan Li

{"title":"Advances in polyethylene biodegradation and bioconversion: Microbial, enzymatic, and biotechnological insights","authors":"Jie Qiao , Luxuan Wu , Xiaoru Ma , Anni Li , Yannan Tian , Hailong Lin , Dongsheng Guo , Xiujuan Li","doi":"10.1016/j.engmic.2025.100255","DOIUrl":"10.1016/j.engmic.2025.100255","url":null,"abstract":"<div><div>Polyethylene (PE) is one of the most widely used plastics worldwide and is valued for its versatility, durability, and cost-effectiveness. However, the chemical stability of PE combined with its widespread use makes it a persistent environmental pollutant that contributes to the accumulation of plastic waste in terrestrial and marine ecosystems. The escalating issue of plastic pollution has underscored the importance of developing sustainable solutions, of which PE biodegradation has emerged as a promising avenue for mitigating the environmental burden of recalcitrant polyolefins. This review systematically summarizes the recent advances in the biodegradation and bioconversion of PE, focusing on methods for evaluating degradation efficiency, the mechanisms by which microorganisms and enzymes contribute to PE degradation, and the microbial and enzymatic resources identified to date. In addition, we discuss physicochemical strategies that enhance degradation efficiency and their integration with biological approaches, as well as the potential applications of emerging biotechnological tools in PE degradation. The integration of cutting-edge biotechnological tools such as synthetic biology and machine learning with traditional biodegradation methods holds great potential for accelerating PE degradation rates and achieving more sustainable plastic waste management.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100255"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145928178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gdx mediates low-affinity Cs⁺/H⁺ antiport and confers cesium resistance in Escherichia coli Gdx介导低亲和力Cs + /H +反端口，并在大肠杆菌中赋予铯抗性

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.engmic.2025.100251

Daiki Kojima , Masahiro Ito

{"title":"Gdx mediates low-affinity Cs⁺/H⁺ antiport and confers cesium resistance in Escherichia coli","authors":"Daiki Kojima , Masahiro Ito","doi":"10.1016/j.engmic.2025.100251","DOIUrl":"10.1016/j.engmic.2025.100251","url":null,"abstract":"<div><div>Following the Fukushima Daiichi nuclear power plant accident, radioactive cesium was released into the environment, prompting intensified efforts to identify cesium-resistant microorganisms. During these studies, we isolated a cesium-resistant <em>Escherichia coli</em> strain, designated ZX-1, which exhibits remarkable tolerance to cesium concentrations exceeding 700 mM. As no prior reports of cesium-resistant <em>E. coli</em> exist, this finding suggests the presence of a previously unrecognized resistance mechanism. This study aims to elucidate the molecular basis of cesium resistance in ZX-1.</div><div>RNA-seq analysis comparing ZX-1 with its parental strain, the commercial <em>E. coli</em> Mach1™, revealed constitutive upregulation of the guanidinium exporter gene <em>gdx</em> in ZX-1. Reanalysis of the whole-genome sequence identified a 20-bp deletion upstream of the <em>gdx</em> open reading frame, likely disrupting formation of the guanidinium riboswitch P2 loop and resulting in constitutive <em>gdx</em> expression.</div><div>To evaluate <em>gdx</em> function, the gene was cloned into the expression vector pBAD24 and expressed in <em>E. coli</em>. The resulting <em>gdx</em>-expressing strain exhibited even greater cesium resistance than ZX-1. Functional assays demonstrated that this strain mediates not only guanidinium/H⁺ antiport activity but also Cs⁺/H⁺ antiport activity. Cesium resistance was further enhanced in the presence of guanidinium, consistent with riboswitch-mediated induction of <em>gdx</em>.</div><div>Collectively, these findings provide evidence for a novel cesium efflux mechanism in <em>E. coli</em> and uncover an unexpected role of the guanidinium exporter Gdx in cesium export. These insights may facilitate the discovery of additional cesium-resistant microorganisms and broaden the potential for future applications.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100251"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145928175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase variation in Bacteroides fragilis governs susceptibility to a microvirus and drives its evolution 脆弱拟杆菌的阶段变异控制着对微病毒的易感性并驱动其进化

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.engmic.2025.100252

Pan Huang , Meiqi Du , Yanqiu Liu , Zhenhao Han , Qian Wan , Fuming Liang , Wenyuan Han

{"title":"Phase variation in Bacteroides fragilis governs susceptibility to a microvirus and drives its evolution","authors":"Pan Huang , Meiqi Du , Yanqiu Liu , Zhenhao Han , Qian Wan , Fuming Liang , Wenyuan Han","doi":"10.1016/j.engmic.2025.100252","DOIUrl":"10.1016/j.engmic.2025.100252","url":null,"abstract":"<div><div>The interaction and co-evolution between human gut bacteria and their phages shape the dynamic gut microbiome, exerting a significant impact on human health. However, the underlying mechanisms are largely unexplored. In particular, a bacteria-phage interaction model of the Bacteroidota phylum and the <em>Microviridae</em> phages is lacking, limiting our understanding of their ecological roles in human gut. In this study, we isolated a Bacteroidota-infecting <em>Microviridae</em> phage φHBP1 from human feces. Infection of its host <em>Bacteroides fragilis</em> with φHBP1 drives multiple genomic structural variations, which are correlated with host resistance to φHBP1. In turn, our phage evolution assay in <em>B. fragilis</em> H1 obtained φHBP1 mutants that carry mutations within the capsid and pilot proteins and can reinfect the resistant bacterial population. Together, our findings provide novel insights into an antagonistic co-evolution mechanism between gut phage and bacteria, and hold important implications for diversifying phages through evolution to target resistant bacteria in phage therapy.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100252"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the PRE configuration reveals the constraints in Ste12 oligomerization in mediating pheromone-inducible transcription in Saccharomyces cerevisiae PRE结构的分析揭示了在酿酒酵母中介导信息素诱导转录的Ste12寡聚化的限制

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-19 DOI: 10.1016/j.engmic.2025.100249

Yiqing Zhang , Yinfeng Wei , Yuxin Huang , Chenyu Wang , Tao Wang , Yujiao Wang , Yingxuan Qi , Guannan Liu

{"title":"Analysis of the PRE configuration reveals the constraints in Ste12 oligomerization in mediating pheromone-inducible transcription in Saccharomyces cerevisiae","authors":"Yiqing Zhang , Yinfeng Wei , Yuxin Huang , Chenyu Wang , Tao Wang , Yujiao Wang , Yingxuan Qi , Guannan Liu","doi":"10.1016/j.engmic.2025.100249","DOIUrl":"10.1016/j.engmic.2025.100249","url":null,"abstract":"<div><div>Yeast cell mating is regulated by the transcription factor Ste12, which activates the transcription of mating genes by binding to pheromone response elements (PREs) in their promoters. PREs vary in number and position among different promoters. However, the effect of PRE organization on Ste12-promoter interactions and the possible downstream transcription of mating genes remains to be fully understood. In this study, we analyzed yeast pheromone-induced gene expression using RNA-seq transcription profiling. We retrieved the promoters of the significantly upregulated genes, focusing on the occurrence and arrangement of PREs. <em>PPRM1</em>, which carries three adjacent consensus PREs, was selected as a model for investigating the relative contribution of each PRE to promoter activity through single-base mutation or deletion. We then evaluated the impact of different PRE organizations on pheromone-induced expression by altering their orientations and copy numbers. Subsequently, we proposed a model to explain the mechanism of transcriptional regulation of pheromone-inducible genes, in which the organization of PREs modulates promoter activity by influencing Ste12 oligomerization. This study paves the way for deciphering the transcriptional mechanisms of eukaryotic regulatory systems.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100249"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147448768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of resistant starch type 5 on gut health through modulating gut microbiota 抗性淀粉5型通过调节肠道菌群对肠道健康的影响

Engineering Microbiology Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.engmic.2025.100250

Raju Ahmmed , Andrew Paff , Lingyan Kong , Songnan Li , Darrell W. Cockburn , Libo Tan

{"title":"Effect of resistant starch type 5 on gut health through modulating gut microbiota","authors":"Raju Ahmmed , Andrew Paff , Lingyan Kong , Songnan Li , Darrell W. Cockburn , Libo Tan","doi":"10.1016/j.engmic.2025.100250","DOIUrl":"10.1016/j.engmic.2025.100250","url":null,"abstract":"<div><div>Resistant starch is a dietary fiber that escapes digestion in the small intestine and undergoes fermentation by gut microbiota in the colon, producing beneficial short-chain fatty acids (SCFAs). Among the various types of resistant starch, resistant starch type 5 (RS5) has gained significant attention due to its unique and stable structural and functional properties. RS5 is a self-assembled V-type inclusion complex formed when amylose helices encapsulate guest molecules. This formation occurs through non-covalent interactions after the native starch structure is disrupted, and a guest compound is introduced. This structure provides enhanced resistance to enzymatic digestion, slows fermentation, and facilitates targeted release of bioactive molecules, making it effective in modulating gut health. RS5 promotes the proliferation of beneficial gut microbiota while suppressing pathogenic species, leading to increased SCFAs production, mostly butyrate, acetate, and propionate, which maintain intestinal integrity, reduces inflammation, and supports metabolic regulation. RS5 also contributes to preventing and managing chronic diseases such as obesity, type 2 diabetes, and colorectal cancer. While prior research has focused on its preparation methods and physicochemical characteristics, the influence of RS5 on gut microbiota and host health remains inadequately explored. This review summarizes the formation, classification, and structural diversity of RS5 complexes and how these factors influence digestibility and fermentation kinetics. Furthermore, it explores how RS5 modulates the composition and metabolic activity of the gut microbiota, enhancing SCFAs production. By comparing RS5 with other RS types, this review highlights its superior prebiotic potential and supports RS5-based functional food development for improving gut and metabolic health, targeting gut microecology.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"6 1","pages":"Article 100250"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0