Engineering Microbiology最新文献

The role of microbiota dysbiosis in Parkinson’s disease: Pathophysiology and therapeutic opportunities 微生物群失调在帕金森病中的作用：病理生理学和治疗机会

Engineering Microbiology Pub Date : 2025-06-28 DOI: 10.1016/j.engmic.2025.100222

Shabnam Santos , Ivonne Salinas , Nicolás Almeida , Andrés Caicedo

{"title":"The role of microbiota dysbiosis in Parkinson’s disease: Pathophysiology and therapeutic opportunities","authors":"Shabnam Santos , Ivonne Salinas , Nicolás Almeida , Andrés Caicedo","doi":"10.1016/j.engmic.2025.100222","DOIUrl":"10.1016/j.engmic.2025.100222","url":null,"abstract":"<div><div>Parkinson’s disease (PD) is a chronic, progressive neurodegenerative disorder characterized by debilitating motor and non-motor symptoms. Its etiology is multifactorial, with no single definitive cause identified, although aging is a significant risk factor. Additional risks include genetic predisposition, family history, and environmental factors such as pesticide exposure and <em>Helicobacter pylori</em> infection. Dysbiosis of the gut microbiota, and in particular bacterial imbalances, has been implicated in the disruption of the gut-brain axis, contributing to both systemic and neuroinflammation. Environmental factors such as antibiotic exposure and toxins can precipitate microbial dysregulation, potentially accelerating PD progression. Understanding the mechanisms of the gut-brain axis and identifying strategies to preserve a healthy microbiome are essential for developing novel therapeutic approaches. This review synthesizes current therapeutic strategies and ongoing research focused on restoring gut-brain balance to combat PD. These approaches include fecal microbiota transplantation, dietary interventions, and probiotic therapies, all of which show promise in mitigating both motor and non-motor symptoms. Furthermore, we emphasize the urgent need for continued research into probiotics and innovative therapeutic approaches for gut-brain axis modulation, presenting novel opportunities for effective PD management.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100222"},"PeriodicalIF":0.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electricity generation and dibenzothiophene biodegradation using a novel electroactive bacterium Lysinibacillus macroides AP in microbial fuel cells 在微生物燃料电池中利用一种新型电活性细菌大内溶杆菌AP发电和降解二苯并噻吩

Engineering Microbiology Pub Date : 2025-06-23 DOI: 10.1016/j.engmic.2025.100221

Zihan Huang, Lei Zhang, Ting Cai, Ruijun Liu, Xiaoyan Qi, Xia Wang

{"title":"Electricity generation and dibenzothiophene biodegradation using a novel electroactive bacterium Lysinibacillus macroides AP in microbial fuel cells","authors":"Zihan Huang, Lei Zhang, Ting Cai, Ruijun Liu, Xiaoyan Qi, Xia Wang","doi":"10.1016/j.engmic.2025.100221","DOIUrl":"10.1016/j.engmic.2025.100221","url":null,"abstract":"<div><div>Polycyclic aromatic sulfur heterocycles, such as dibenzothiophene (DBT), and their alkylated derivatives are recognized as persistent and toxic contaminants that pose major risks to the environment and human health. Here, a novel electroactive gram-positive bacterium, <em>Lysinibacillus macroides</em> AP, was isolated and identified from a microbial fuel cell (MFC) powered by aromatic compounds. An electricity generation performance with a maximum discharge voltage of 424.59 mV and a power density of 420.95 mW m⁻<sup>2</sup> was obtained using <em>L. macroides</em> AP in an MFC fueled with sodium formate. An analysis of the extracellular electron transfer (EET) mechanism indicated that the endogenous redox mediators produced by <em>L. macroides</em> AP were not detected, but exogenous redox mediators such as thionine acetate and anthraquinone-2, 6-disulfonate could temporarily enhance EET. The characterization of biofilm morphology revealed a dense network of microbial nanowires on the cell surface of <em>L. macroides</em> AP; the abundance of these nanowires was positively correlated with the discharge efficiency of the MFC, suggesting that the nanowires generated by <em>L. macroides</em> AP cells were likely to promote EET. Additionally, effective bioelectricity generation and simultaneous DBT degradation were successfully achieved using <em>L. macroides</em> AP in MFCs, with a power density of 385.20 mW m⁻<sup>2</sup> and 88.72 % DBT removal. This is the first report on a novel ecological role of <em>L. macroides</em> AP as a gram-positive electroactive bacterium, emphasizing its potential applications in environmental remediation and energy recovery.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 4","pages":"Article 100221"},"PeriodicalIF":0.0,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering thermotolerant microbial strains via TrRCC1 overexpression for efficient bioethanol production 通过TrRCC1过表达工程耐热微生物菌株高效生产生物乙醇

Engineering Microbiology Pub Date : 2025-06-01 DOI: 10.1016/j.engmic.2025.100212

Tingting Chen, Xiao He, Xinyan Zhang, Tian Tian, Jian Cheng, Tingting Long, Yonghao Li

{"title":"Engineering thermotolerant microbial strains via TrRCC1 overexpression for efficient bioethanol production","authors":"Tingting Chen, Xiao He, Xinyan Zhang, Tian Tian, Jian Cheng, Tingting Long, Yonghao Li","doi":"10.1016/j.engmic.2025.100212","DOIUrl":"10.1016/j.engmic.2025.100212","url":null,"abstract":"<div><div>Efficient conversion of corn stover to bioethanol via simultaneous saccharification and fermentation (SSF) is a promising strategy for sustainable biofuel production. A major current barrier to this process is the limited thermotolerance of <em>Saccharomyces cerevisiae</em>, which hampers its performance under the high-temperature conditions required for efficient SSF. In this study, we identified <em>TrRCC1</em>, a gene from <em>Trichoderma reesei</em>, as a candidate for improving microbial stress resistance. Overexpression of <em>TrRCC1</em> in both <em>T. reesei</em> Rut C30 and <em>S. cerevisiae</em> BY4741 significantly enhanced thermotolerance. In <em>T. reesei</em> Rut C30, <em>TrRCC1</em> overexpression improved heat resistance and increased cellulase production by 2.5-fold compared to the wild-type strain. In <em>S. cerevisiae</em> BY4741, <em>TrRCC1</em> overexpression resulted in enhanced thermotolerance and a 21.8 % increase in ethanol production during SSF of corn stover. The ethanol concentration achieved in the SSF process with <em>TrRCC1</em>-overexpressing <em>S. cerevisiae</em> was 44.1 g/L, which was a notable improvement over control strain production. These findings highlight the potential of <em>TrRCC1</em> as a key gene for engineering microbial strains with improved stress resistance to enhance the efficiency of bioethanol production from lignocellulosic biomass.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100212"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional heterologous expression of the reversible Cu-decarboxylase from the lichen, Cladonia uncialis 地衣中可逆cu脱羧酶的功能异源表达

Engineering Microbiology Pub Date : 2025-06-01 DOI: 10.1016/j.engmic.2025.100211

Harman Gill, John L. Sorensen

{"title":"Functional heterologous expression of the reversible Cu-decarboxylase from the lichen, Cladonia uncialis","authors":"Harman Gill, John L. Sorensen","doi":"10.1016/j.engmic.2025.100211","DOIUrl":"10.1016/j.engmic.2025.100211","url":null,"abstract":"<div><div>Despite the isolation of over 1000 known bioactive lichen mycobiont-derived secondary metabolites (SMs), understanding the genetic basis of their biosynthesis remains elusive. Biosynthetic gene clusters (BGCs) have been tentatively linked to chemical structures, with core genes such as polyketide synthases (PKSs) surrounded by accessory genes like decarboxylases. In this study, we focused on a decarboxylase gene from the genome of the lichen <em>cladonia uncialis</em> (named as <em>Cu</em>-decarboxylase) to elucidate its role in SM biosynthesis. A 963 bp gene was cloned from <em>C. uncialis</em> and expressed in <em>Escherichia coli</em> (BL21(DE3) cells using the pQE80L expression vector. The resulting 35 kDa protein was purified by applying a Ni<sup>+</sup>-NTA column using an FPLC system. Functional activity assays revealed the decarboxylation and reversible carboxylation of resorcinol to 2,4-dihydroxybenzoic acid and orcinol to orsellinic acid. This suggests a potential role for this <em>Cu</em>-decarboxylase in SM biosynthesis.</div><div>Furthermore, the lack of activity on substrates like anthranilic acid and aniline highlighted the importance of the phenolic OH group in facilitating these reactions. The 3D protein structure was predicted with AlphaFold3, based on sequence similarity with a known decarboxylases and revealed the importance of a zinc cofactor for the catalytic activity of the enzyme. The optimization of the reaction conditions, particularly for orsellinic acid production from orcinol, may enhance conversion rates and offer a viable route for industrial-scale production of bioactive compounds. This study marks the first known instance of functional heterologous expression of a non-codon-optimized gene isolated from lichen in <em>E. coli</em>.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100211"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to “Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines” [Engineering Microbiology 4 (2024) 100162] “蛋白质热稳定性的调控及其在热减毒疫苗开发中的潜在应用”的勘误[工程微生物学4 (2024)100162]

Engineering Microbiology Pub Date : 2025-06-01 DOI: 10.1016/j.engmic.2025.100204

Maofeng Wang , Cancan Wu , Nan Liu , Xiaoqiong Jiang , Hongjie Dong , Shubao Zhao , Chaonan Li , Sujuan Xu , Lichuan Gu

引用次数: 0

Superoxide-mediated O2 activation drives radical cyclization in ergot alkaloid biosynthesis 超氧化物介导的O2活化驱动麦角生物碱生物合成中的自由基环化

Engineering Microbiology Pub Date : 2025-06-01 DOI: 10.1016/j.engmic.2025.100207

Yuanyuan Jiang , Zhong Li , Shengying Li

引用次数: 0

The Kongming defense: Host-pathogen battles take a new face 孔明防御：宿主-病原体的战斗有了新的面貌

Engineering Microbiology Pub Date : 2025-06-01 DOI: 10.1016/j.engmic.2025.100209

Dongchun Ni

引用次数: 0

Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis 五烯内酯生物合成中细胞色素p450催化烯丙基氧化五烯烯生成1-脱氧五烯酸

Engineering Microbiology Pub Date : 2025-06-01 DOI: 10.1016/j.engmic.2025.100206

Jing Li , Chengde Zhang , Shiwen Wu , Jiao Xue , Ke Chen , Zixin Deng , Dongqing Zhu

引用次数: 0

Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-free in vitro biosynthetic system 利用无辅助因子和无共底物的体外生物合成系统从ω-氨基酸生产二羧酸盐

Engineering Microbiology Pub Date : 2025-05-17 DOI: 10.1016/j.engmic.2025.100210

Jinxin Yan , Hui Zhang , Hongxu Zhang , Hairong Yu , Wenjia Tian , Mingyuan Liu , Weikang Sun , Leilei Guo , Xiaoxu Tan , Kaiyu Gao , Tianyi Jiang , Chuanjuan Lü , Qianjin Kang , Wensi Meng , Cuiqing Ma , Chao Gao , Ping Xu

{"title":"Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-free in vitro biosynthetic system","authors":"Jinxin Yan , Hui Zhang , Hongxu Zhang , Hairong Yu , Wenjia Tian , Mingyuan Liu , Weikang Sun , Leilei Guo , Xiaoxu Tan , Kaiyu Gao , Tianyi Jiang , Chuanjuan Lü , Qianjin Kang , Wensi Meng , Cuiqing Ma , Chao Gao , Ping Xu","doi":"10.1016/j.engmic.2025.100210","DOIUrl":"10.1016/j.engmic.2025.100210","url":null,"abstract":"<div><div>Dicarboxylates are valuable platform compounds with a broad range of applications. The <em>in vitro</em> biosynthetic system used to produce dicarboxylates from ω-amino acids via the natural pathway requires costly cofactors and co-substrates, which restricts its economic feasibility of use. In this study, we designed a cofactor- and co-substrate-free artificial pathway for the production of dicarboxylates from ω-amino acids. Only three enzymes (viz., amine oxidase from <em>Kluyveromyces marxianus</em> DMKU3-1042, xanthine oxidase from bovine milk, and catalase from <em>Aspergillus niger</em>) were required for dicarboxylate production. Succinate (0.79 g g<sup>-1</sup>), glutarate (0.83 g g<sup>-1</sup>), and adipate (0.77 g g<sup>-1</sup>) were produced in high yields from the corresponding ω-amino acids through the <em>in vitro</em> biosynthetic system with the artificial pathway. Glutarate could also be produced from <span>l</span>-lysine by further introducing <span>l</span>-lysine monooxygenase and 5-aminovaleramide amidohydrolase from <em>Pseudomonas putida</em> KT2440 into the <em>in vitro</em> biosynthetic system, with the cofactor- and co-substrate-free system achieving a product yield of 0.63 g g<sup>-1</sup>. Considering its desirable characteristics, this artificial pathway-based <em>in vitro</em> biosynthetic system may be a promising alternative for dicarboxylate production from biotechnologically produced ω-amino acids.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100210"},"PeriodicalIF":0.0,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of single-cell RNA sequencing data to examine the gastric inflammation-to-cancer transition and evaluation of the effect of probiotic on precancerous lesions 单细胞RNA测序数据分析胃炎症-癌转化及益生菌对癌前病变影响的评价

Engineering Microbiology Pub Date : 2025-05-09 DOI: 10.1016/j.engmic.2025.100208

Minmin Hu , Shiyang Xu , Ruofei Xu , Xiangjie Qi , Xiaofeng Yu , Jinqi Wang , Yige Li , Yangyang Liu , Guiran Xi , Junbao Yu , Mei Shi

{"title":"Analysis of single-cell RNA sequencing data to examine the gastric inflammation-to-cancer transition and evaluation of the effect of probiotic on precancerous lesions","authors":"Minmin Hu , Shiyang Xu , Ruofei Xu , Xiangjie Qi , Xiaofeng Yu , Jinqi Wang , Yige Li , Yangyang Liu , Guiran Xi , Junbao Yu , Mei Shi","doi":"10.1016/j.engmic.2025.100208","DOIUrl":"10.1016/j.engmic.2025.100208","url":null,"abstract":"<div><div>Gastric cancer (GC) is the fifth most prevalent malignancy globally. However, its heterogeneity and asymptomatic early-stage development hinder timely diagnosis and effective treatment. Here, we employed single-cell RNA sequencing to delineate the transitional features of pit mucous cells (PMCs) during the gastritis-to-cancer transition and identified 100 core genes. Characterization of the gene set revealed the role of ribosomal protein small subunit and ribosomal protein large subunit in inflammation-to-cancer transition, which promoted ribonucleoprotein complex biogenesis and cytoplasmic translation. External validation using independent cohorts confirmed that this core gene set discriminated disease progression (AUC > 0.7) and was significantly enriched in GC tissues (<em>p</em> < 0.01). Moreover, we evaluated the therapeutic intervention effects of <em>C. butyricum</em> and synbiotics (Weichanghao®) using a rat model of gastritis and demonstrated the targeted suppression of inflammation-to-cancer transition genes. Our findings establish the basis for early diagnosis of GC through PMC-driven molecular dynamics. Additionally, we propose microbiota-based strategies to prevent the inflammation-to-cancer transition in preneoplastic stages. Furthermore, our results highlight that dysbiosis of the gastric microbiome can be addressed using probiotic supplementations and the core gene set may provide labeling for the evaluation of probiotics-based treatment.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 3","pages":"Article 100208"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0