Engineering Microbiology最新文献_第10页

Sustainable biosynthesis of valuable diterpenes in microbes 微生物中有价值二萜的可持续生物合成

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100058

Yanbin Liu, Xixian Chen, Congqiang Zhang

{"title":"Sustainable biosynthesis of valuable diterpenes in microbes","authors":"Yanbin Liu, Xixian Chen, Congqiang Zhang","doi":"10.1016/j.engmic.2022.100058","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100058","url":null,"abstract":"<div><p>Diterpenes, or diterpenoids, are the most abundant and diverse subgroup of terpenoids, the largest family of secondary metabolites. Most diterpenes possess broad biological activities including anti-inflammatory, antiviral, anti-tumoral, antimicrobial, anticancer, antifungal, antidiabetic, cardiovascular protective, and phytohormone activities. As such, diterpenes have wide applications in medicine (e.g., the anticancer drug Taxol and the antibiotic pleuromutilin), agriculture (especially as phytohormones such as gibberellins), personal care (e.g., the fragrance sclareol) and food (e.g., steviol glucosides as low-calorie sweeteners) industries. Diterpenes are biosynthesized in a common route with various diterpene synthases and decoration enzymes like cytochrome P450 oxidases, glycosidases, and acyltransferases. Recent advances in DNA sequencing and synthesis, omics analysis, synthetic biology, and metabolic engineering have enabled efficient production of diterpenes in several chassis hosts like <em>Escherichia coli, Saccharomyces cerevisiae, Yarrowia lipolytica, Rhodosporidium toruloides</em>, and <em>Fusarium fujikuroi</em>. This review summarizes the recently discovered diterpenes, their related enzymes and biosynthetic pathways, particularly highlighting the microbial synthesis of high-value diterpenes directly from inexpensive carbon sources (e.g., sugars). The high titers (>4 g/L) achieved mean that some of these endeavors are reaching or close to commercialization. As such, we envisage a bright future in translating microbial synthesis of diterpenes into commercialization.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100058"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods of DNA introduction for the engineering of commensal microbes 共生微生物工程中的DNA导入方法

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100048

Dake Liu , Nicole E. Siguenza , Amir Zarrinpar , Yousong Ding

引用次数: 3

Metabolic engineering using acetate as a promising building block for the production of bio‐based chemicals 利用乙酸盐作为生产生物基化学品的有前景的构建块的代谢工程

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100036

Guiping Gong , Bo Wu , Linpei Liu, Jianting Li, Qili Zhu, Mingxiong He, Guoquan Hu

{"title":"Metabolic engineering using acetate as a promising building block for the production of bio‐based chemicals","authors":"Guiping Gong , Bo Wu , Linpei Liu, Jianting Li, Qili Zhu, Mingxiong He, Guoquan Hu","doi":"10.1016/j.engmic.2022.100036","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100036","url":null,"abstract":"<div><p>The production of biofuels and biochemicals derived from microbial fermentation has received a lot of attention and interest in light of concerns about the depletion of fossil fuel resources and climatic degeneration. However, the economic viability of feedstocks for biological conversion remains a barrier, urging researchers to develop renewable and sustainable low-cost carbon sources for future bioindustries. Owing to the numerous advantages, acetate has been regarded as a promising feedstock targeting the production of acetyl-CoA-derived chemicals. This review aims to highlight the potential of acetate as a building block in industrial biotechnology for the production of bio-based chemicals with metabolic engineering. Different alternative approaches and routes comprised of lignocellulosic biomass, waste streams, and C1 gas for acetate generation are briefly described and evaluated. Then, a thorough explanation of the metabolic pathway for biotechnological acetate conversion, cellular transport, and toxin tolerance is described. Particularly, current developments in metabolic engineering of the manufacture of biochemicals from acetate are summarized in detail, with various microbial cell factories and strategies proposed to improve acetate assimilation and enhance product formation. Challenges and future development for acetate generation and assimilation as well as chemicals production from acetate is eventually shown. This review provides an overview of the current status of acetate utilization and proves the great potential of acetate with metabolic engineering in industrial biotechnology.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"2 4","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370322000273/pdfft?md5=088f7fc51c1719804dc0bdd1323b2ec2&pid=1-s2.0-S2667370322000273-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71894964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Lysine acetylation decreases enzyme activity and protein level of Escherichia coli lactate dehydrogenase 赖氨酸乙酰化降低大肠杆菌乳酸脱氢酶的酶活性和蛋白质水平

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100045

Min Liu , Meitong Huo , Likun Guo , Yingxin Fu , Mo Xian , Qingsheng Qi , Wei Liu , Guang Zhao

{"title":"Lysine acetylation decreases enzyme activity and protein level of Escherichia coli lactate dehydrogenase","authors":"Min Liu , Meitong Huo , Likun Guo , Yingxin Fu , Mo Xian , Qingsheng Qi , Wei Liu , Guang Zhao","doi":"10.1016/j.engmic.2022.100045","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100045","url":null,"abstract":"<div><p>Lactate is an important bulk chemical with widespread applications and a major byproduct of other chemicals bioprocess in microbial fermentation. Lactate dehydrogenase A (LdhA) catalyzes the synthesis of lactate from pyruvate. Lysine acetylation is an evolutionarily conserved post-translational modification; however, the mechanisms underlying the regulation of LdhA function by lysine acetylation in <em>Escherichia coli</em> remain poorly understood. Herein, we demonstrate acetylation of <em>E. coli</em> LdhA occurs via enzymatic and non-enzymatic mechanisms. Further, we show carbon source type and concentration affect the lysine acetylation status of LdhA via a non-enzymatic mechanism. Lysine acetylation significantly inhibits the enzymatic activity and protein level of LdhA. The results of the present study demonstrate lysine acetylation of <em>E. coli</em> LdhA is irreversible. Understanding of the effects of lysine acetylation on LdhA function may provide a new perspective for regulating lactate production in microbial synthesis.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"2 4","pages":"Article 100045"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370322000364/pdfft?md5=c8b18f6fe6bad875cc8ba439238b6b55&pid=1-s2.0-S2667370322000364-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71894963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioproduction of monoterpene indole alkaloids in a single cell factory 单细胞工厂中单萜吲哚生物碱的生物生产

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100050

Jianping Huang , Shengxiong Huang

引用次数: 3

Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination Cascade-Cas3利用噬菌体编码的同源重组促进假单胞菌的高精度基因组工程

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100046

Wentao Zheng , Yandong Xia , Xue Wang , Shiqing Gao , Diao Zhou , Jun Fu , Ruijuan Li , Jia Yin

引用次数: 4

Recent developments in the identification and biosynthesis of antitumor drugs derived from microorganisms 微生物来源的抗肿瘤药物的鉴定和生物合成的最新进展

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100047

Qi Gao , Sizhe Deng , Tianyu Jiang

引用次数: 2

Aquaculture sustainability through alternative dietary ingredients: Microalgal value-added products 通过替代膳食成分实现水产养殖的可持续性:微藻增值产品

Engineering Microbiology Pub Date : 2022-12-01 DOI: 10.1016/j.engmic.2022.100049

John N. Idenyi , Jonathan C. Eya , Amechi S. Nwankwegu , Emeka G. Nwoba

{"title":"Aquaculture sustainability through alternative dietary ingredients: Microalgal value-added products","authors":"John N. Idenyi , Jonathan C. Eya , Amechi S. Nwankwegu , Emeka G. Nwoba","doi":"10.1016/j.engmic.2022.100049","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100049","url":null,"abstract":"<div><p>Aquaculture contributes remarkably to the global economy and food security through seafood production, an important part of the global food supply chain. The success of this industry depends heavily on aquafeeds, and the nutritional composition of the feed is an important factor for the quality, productivity, and profitability of aquaculture species. The sustainability of the aquaculture industry depends on the accessibility of quality feed ingredients, such as fishmeal and fish oil. These traditional feedstuffs are under increasing significant pressure due to the rapid expansion of aquaculture for human consumption and the decline of natural fish harvest. In this review, we evaluated the development of microalgal molecules in aquaculture and expanded the use of these high-value compounds in the production of aquaculture diets. Microalgae-derived functional ingredients emerged as one of the promising alternatives for aquafeed production with positive health benefits. Several compounds found in microalgae, including carotenoids (lutein, astaxanthin, and β-carotene), essential amino acids (leucine, valine, and threonine), β-1–3-glucan, essential oils (docosahexaenoic acid and eicosapentaenoic acid), minerals, and vitamins, are of high nutritional value to aquaculture.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"2 4","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370322000406/pdfft?md5=06265d972f4fa1627cbf4a7604ac81c7&pid=1-s2.0-S2667370322000406-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71894967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Bioproduction of monoterpene indole alkaloids in a single cell factory. 单细胞工厂中单萜吲哚生物碱的生物生产。

Engineering Microbiology Pub Date : 2022-10-01 eCollection Date: 2022-12-01 DOI: 10.1016/j.engmic.2022.100050

Jianping Huang, Shengxiong Huang

引用次数: 0

Aquaculture sustainability through alternative dietary ingredients: Microalgal value-added products. 通过替代膳食成分实现水产养殖的可持续性：微藻增值产品。

Engineering Microbiology Pub Date : 2022-09-21 eCollection Date: 2022-12-01 DOI: 10.1016/j.engmic.2022.100049

John N Idenyi, Jonathan C Eya, Amechi S Nwankwegu, Emeka G Nwoba

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引用次数: 0