Engineering Microbiology最新文献_第9页

A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency 一种通过引入额外的切割位点来提高Vip3Aa蛋白水解激活效率来提高其杀虫效力的策略

Engineering Microbiology Pub Date : 2023-03-17 DOI: 10.1016/j.engmic.2023.100083

Kun Jiang , Zhe Chen , Yiting Shi , Yuanrong Zang , Chengbin Shang , Xi Huang , Jiahe Zang , Zhudong Bai , Xuyao Jiao , Jun Cai , Xiang Gao

{"title":"A strategy to enhance the insecticidal potency of Vip3Aa by introducing additional cleavage sites to increase its proteolytic activation efficiency","authors":"Kun Jiang , Zhe Chen , Yiting Shi , Yuanrong Zang , Chengbin Shang , Xi Huang , Jiahe Zang , Zhudong Bai , Xuyao Jiao , Jun Cai , Xiang Gao","doi":"10.1016/j.engmic.2023.100083","DOIUrl":"https://doi.org/10.1016/j.engmic.2023.100083","url":null,"abstract":"<div><p>Microbially derived, protein-based biopesticides have become a vital element in pest management strategies. Vip3 family proteins from <em>Bacillus thuringiensis</em> have distinct characteristics from known insecticidal Cry toxins and show efficient insecticidal activity against several detrimental lepidopteran pests. They are considered to be a promising toxic candidate for the management of various detrimental pests. In this study, we found that in addition to the preliminary digestion sites lysine, there are multiple cleavage activation sites in the linker region between domain I (DI) and DII of Vip3Aa. We further demonstrated that by adding more cleavage sites between DI and DII of Vip3Aa, its proteolysis efficiency by midgut proteases can be significantly increased, and correspondingly enhance its insecticidal activity against <em>Spodoptera frugiperda</em> and <em>Helicoverpa armigera</em> larvae. Our study promotes the understanding of the insecticidal mechanism of Vip3 proteins and illustrates an easily implementable strategy to increase the insecticidal potency of Vip3Aa. This facilitates their potential future development and efficient application for sustainable agriculture.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 4","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative modification of free-standing amino acids by Fe(II)/αKG-dependent oxygenases Fe（II）/αKG依赖性加氧酶对独立氨基酸的氧化修饰

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100062

Hui Tao , Ikuro Abe

{"title":"Oxidative modification of free-standing amino acids by Fe(II)/αKG-dependent oxygenases","authors":"Hui Tao , Ikuro Abe","doi":"10.1016/j.engmic.2022.100062","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100062","url":null,"abstract":"<div><p>Fe(II)/α-ketoglutarate (αKG)-dependent oxygenases catalyze the oxidative modification of various molecules, from DNA, RNA, and proteins to primary and secondary metabolites. They also catalyze a variety of biochemical reactions, including hydroxylation, halogenation, desaturation, epoxidation, cyclization, peroxidation, epimerization, and rearrangement. Given the versatile catalytic capability of such oxygenases, numerous studies have been conducted to characterize their functions and elucidate their structure–function relationships over the past few decades. Amino acids, particularly nonproteinogenic amino acids, are considered as important building blocks for chemical synthesis and components for natural product biosynthesis. In addition, the Fe(II)/αKG-dependent oxygenase superfamily includes important enzymes for generating amino acid derivatives, as they efficiently modify various free-standing amino acids. The recent discovery of new Fe(II)/αKG-dependent oxygenases and the repurposing of known enzymes in this superfamily have promoted the generation of useful amino acid derivatives. Therefore, this study will focus on the recent progress achieved from 2019 to 2022 to provide a clear view of the mechanism by which these enzymes have expanded the repertoire of free amino acid oxidative modifications.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100062"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors 双组分系统GacS/GacA，细菌生理行为的全局反应调节因子

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100051

Huihui Song , Yuying Li , Yan Wang

{"title":"Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors","authors":"Huihui Song , Yuying Li , Yan Wang","doi":"10.1016/j.engmic.2022.100051","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100051","url":null,"abstract":"<div><p>The signal transduction system of microorganisms helps them adapt to changes in their complex living environment. Two-component system (TCS) is a representative signal transduction system that plays a crucial role in regulating cellular communication and secondary metabolism. In Gram-negative bacteria, an unorthodox TCS consisting of histidine kinase protein GacS (initially called LemA) and response regulatory protein GacA is widespread. It mainly regulates various physiological activities and behaviors of bacteria, such as quorum sensing, secondary metabolism, biofilm formation and motility, through the Gac/Rsm (<u>R</u>egulator of <u>s</u>econdary <u>m</u>etabolism) signaling cascade pathway. The global regulatory ability of GacS/GacA in cell physiological activities makes it a potential research entry point for developing natural products and addressing antibiotic resistance. In this review, we summarize the progress of research on GacS/GacA from various perspectives, including the reaction mechanism, related regulatory pathways, main functions and GacS/GacA-mediated applications. Hopefully, this review will facilitate further research on GacS/GacA and promote its application in regulating secondary metabolism and as a therapeutic target.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Next-generation synthetic biology approaches for the accelerated discovery of microbial natural products 加速发现微生物天然产物的下一代合成生物学方法

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100060

Lei Li

{"title":"Next-generation synthetic biology approaches for the accelerated discovery of microbial natural products","authors":"Lei Li","doi":"10.1016/j.engmic.2022.100060","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100060","url":null,"abstract":"<div><p>Microbial natural products (NPs) and their derivates have been widely used in health care and agriculture during the past few decades. Although large-scale bacterial or fungal (meta)genomic mining has revealed the tremendous biosynthetic potentials to produce novel small molecules, there remains a lack of universal approaches to link NP biosynthetic gene clusters (BGCs) to their associated products at a large scale and speed. In the last ten years, a series of emerging technologies have been established alongside the developments in synthetic biology to engineer cryptic metabolite BGCs and edit host genomes. Diverse computational tools, such as antiSMASH and PRISM, have also been simultaneously developed to rapidly identify BGCs and predict the chemical structures of their products. This review discusses the recent developments and trends pertaining to the accelerated discovery of microbial NPs driven by a wide variety of next-generation synthetic biology approaches, with an emphasis on the <em>in situ</em> activation of silent BGCs at scale, the direct cloning or refactoring of BGCs of interest for heterologous expression, and the synthetic-bioinformatic natural products (syn-BNP) approach for the guided rapid access of bioactive non-ribosomal peptides.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Constitutive overexpression of cellobiohydrolase 2 in Trichoderma reesei reveals its ability to initiate cellulose degradation 里氏木霉纤维生物水解酶2的组成型过表达揭示了其引发纤维素降解的能力

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100059

Yubo Wang , Meibin Ren , Yifan Wang , Lu Wang , Hong Liu , Mei Shi , Yaohua Zhong

{"title":"Constitutive overexpression of cellobiohydrolase 2 in Trichoderma reesei reveals its ability to initiate cellulose degradation","authors":"Yubo Wang , Meibin Ren , Yifan Wang , Lu Wang , Hong Liu , Mei Shi , Yaohua Zhong","doi":"10.1016/j.engmic.2022.100059","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100059","url":null,"abstract":"<div><p>Cellulose degradation results from the synergistic effect of different enzymes, but which enzyme is involved in the initial stage of cellulose degradation is still not well understood. Cellobiohydrolase 2 (CBH2) attached to the conidial surface is possibly associated with the initial stage. However, its specific mechanism is still incompletely known. This study explored the potential role of CBH2 in initiating cellulose degradation using a constitutive overexpression strategy. First, the CBH2-overexpression <em>Trichoderma reesei</em> strains Qgc2–5 and Qrc2–40 were constructed using the constitutive promoters P<em>gpd1</em> and P<em>rpS30</em>, respectively. It was found that <em>cbh2</em> was expressed at a high level under the glucose conditions and was significantly higher than that of the parental strain QM9414 at the early stage of 29 h when cellulose was used as the carbon source. Particularly, the constitutive overexpression of <em>cbh2</em> caused the strong expression of major cellulase-encoding genes (<em>cbh1, eg1</em>, and <em>eg2</em>) and the rapid decomposition of cellulosic material. Meanwhile, the scanning electron microscope showed that the groove-like structure of the cellulose surface was eroded seriously owing to CBH2 overexpression, which caused the cellulose surface to be smooth. These results showed that the overexpression of CHB2 caused the major cellulase enzymes to be expressed and contributed to cellulose degradation, showing the potential role of CBH2 in the initial stage of the cellulose hydrolytic process.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100059"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Synergistic antifungal mechanism of effective components from essential oil against Penicillium roqueforti 精油有效成分对罗克福青霉菌的协同抗真菌机制

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100057

Fangyuan Zhao , Qianyu Li , Hao Wu , Jinglin Huang , Jian Ju

{"title":"Synergistic antifungal mechanism of effective components from essential oil against Penicillium roqueforti","authors":"Fangyuan Zhao , Qianyu Li , Hao Wu , Jinglin Huang , Jian Ju","doi":"10.1016/j.engmic.2022.100057","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100057","url":null,"abstract":"<div><p>Essential oil (EO) has significant antifungal activity. However, there is limited information on the mechanism of the synergistic antifungal effect of the effective components of EO against fungi. In the present study, molecular electrostatic potential and molecular docking were used for the first time to investigate the synergistic antifungal mechanism of eugenol and citral small molecule (C<sub>EC</sub>) against <em>Penicillium roqueforti</em>. The results showed that the C<sub>EC</sub> treatment made the activity of β-(1,3)-glucan synthase (GS) and chitin synthase (CS) decreas by 20.2% and 11.1%, respectively, and the contents of which decreased by 85.0% and 27.9%, respectively compared with the control group. Molecular docking revealed that C<sub>EC</sub> small molecules could bind to GS and CS through different amino acid residues, inhibiting their activity and synthesis. The C<sub>EC</sub> can combine with tryptophan, tyrosine, and phenylalanine in the cell membrane, causing damage to the cell membrane. The binding sites between small molecules and amino acids were mainly around the OH group. In addition, C<sub>EC</sub> affected the energy metabolism system and inhibited the glycolysis pathway<em>.</em> Simultaneously, C<sub>EC</sub> treatment reduced the ergosterol content in the cell membrane by 58.2% compared with the control group. Finally, changes in β-galactosidase, metal ion leakage, and relative conductivity confirmed the destruction of the cell membrane, which resulted in the leakage of cell contents. The above results showed that C<sub>EC</sub> can kill <em>P. roqueforti</em> by inhibiting energy metabolism and destroying the integrity of the cell membrane.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100057"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50204328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Recent advances in screening amino acid overproducers 筛选氨基酸过量生产菌的研究进展

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100066

Junkai Tuo , Said Nawab , Xiaoyan Ma , Yi-Xin Huo

{"title":"Recent advances in screening amino acid overproducers","authors":"Junkai Tuo , Said Nawab , Xiaoyan Ma , Yi-Xin Huo","doi":"10.1016/j.engmic.2022.100066","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100066","url":null,"abstract":"<div><p>Microbial fermentation has contributed to 80% of global amino acid production. The key to microbial fermentation is to obtain fermentation strains with high performance to produce target amino acids with a high yield. These strains are primarily derived from screening enormous mutant libraries. Therefore, a high-throughput, rapid, accurate, and universal screening strategy for amino acid overproducers has become a guarantee for obtaining optional amino acid overproducers. In recent years, the rapid development of various novel screening strategies has been witnessed. However, proper analysis and discussion of these innovative technologies are lacking. Here we systematically reviewed recent advances in screening strategies: the auxotrophic-based strategy, the biosensor-based strategy, and the latest translation-based screening strategy. The design principle, application scope, working efficiency, screening accuracy, and universality of these strategies were discussed in detail. The potential for screening nonstandard amino acid overproducers was also analyzed. Guidance for the improvement of future screening strategies is provided in this review, which could expedite the reconstruction of amino acid overproducers and help promote the fermentation industry to reduce cost, increase yield, and improve quality.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100066"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Natural and engineered cyclodipeptides: Biosynthesis, chemical diversity, and engineering strategies for diversification and high-yield bioproduction. 天然和工程环二肽：生物合成、化学多样性和多样化和高产生物生产的工程策略。

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100067

Wahyu Setia Widodo , Sonja Billerbeck

{"title":"Natural and engineered cyclodipeptides: Biosynthesis, chemical diversity, and engineering strategies for diversification and high-yield bioproduction.","authors":"Wahyu Setia Widodo , Sonja Billerbeck","doi":"10.1016/j.engmic.2022.100067","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100067","url":null,"abstract":"<div><p>Cyclodipeptides are diverse chemical scaffolds that show a broad range of bioactivities relevant for medicine, agriculture, chemical catalysis, and material sciences. Cyclodipeptides can be synthesized enzymatically through two unrelated enzyme families, non-ribosomal peptide synthetases (NRPS) and cyclodipeptide synthases (CDPSs). The chemical diversity of cyclodipeptides is derived from the two amino acid <strong>side</strong> chains and the modification of those side-chains by cyclodipeptide tailoring enzymes. While a large spectrum of chemical diversity is already known today, additional chemical space - and as such potential new bioactivities - could be accessed by exploring yet undiscovered NRPS and CDPS gene clusters as well as via engineering. Further, to exploit cyclodipeptides for applications, the low yield of natural biosynthesis needs to be overcome. In this review we summarize current knowledge on NRPS and CDPS-based cyclodipeptide biosynthesis, engineering approaches to further diversity the natural chemical diversity as well as strategies for high-yield production of cyclodipeptides, including a discussion of how advancements in synthetic biology and metabolic engineering can accelerate the translational potential of cyclodipeptides.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100067"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Challenges and opportunities for third-generation ethanol production: A critical review 第三代乙醇生产面临的挑战和机遇：综述

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100056

Caroline Müller , Thamarys Scapini , Alan Rempel , Ederson Rossi Abaide , Aline Frumi Camargo , Mateus Torres Nazari , Viviani Tadioto , Charline Bonatto , Marcus Vinícius Tres , Giovani Leone Zabot , Luciane Maria Colla , Helen Treichel , Sérgio Luiz Alves Jr.

{"title":"Challenges and opportunities for third-generation ethanol production: A critical review","authors":"Caroline Müller , Thamarys Scapini , Alan Rempel , Ederson Rossi Abaide , Aline Frumi Camargo , Mateus Torres Nazari , Viviani Tadioto , Charline Bonatto , Marcus Vinícius Tres , Giovani Leone Zabot , Luciane Maria Colla , Helen Treichel , Sérgio Luiz Alves Jr.","doi":"10.1016/j.engmic.2022.100056","DOIUrl":"https://doi.org/10.1016/j.engmic.2022.100056","url":null,"abstract":"<div><p>In recent decades, third-generation (3G) biofuels have become a more attractive method of fuel production, as algae cultivation does not infringe on resources needed for food production. Additionally, algae can adapt to different environments, has high photosynthetic efficiency (CO<sub>2</sub> fixation), and has a high potential for carbohydrate accumulation. The prevalence of algae worldwide demonstrates its ability to adapt to different environments and climates, proving its biodiversity and versatility. Algae can be grown in wastewater, seawater, and even sewage, thus ensuring a lower water footprint and greater energy efficiency during algal biomass production. Because of this, the optimization of 3G ethanol production appears to be an excellent alternative to mitigate environmental impacts and increase energy and food security. This critical review presents (i) the stages of cultivation and processing of micro and macroalgae; (ii) the selection of yeasts (through engineering and/or bioprospecting) to produce ethanol from these biomasses; (iii) the potential of seawater-based facilities to reduce water footprint; and (iv) the mass and energy balances of 3G ethanol production in the world energy matrix. This article is, above all, a brainstorm on the environmental viability of algae bioethanol.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"3 1","pages":"Article 100056"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50203895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Distinct dynamic phases observed in bacterial microcosms 在细菌微宇宙中观察到不同的动力学阶段

Engineering Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.engmic.2022.100063

Andrea Aparicio , Yang-Yu Liu

引用次数: 1