Engineering Microbiology最新文献_第4页

Production of cycloastragenol in metabolically engineered yeast 代谢工程酵母中环黄芪醇的生产

Engineering Microbiology Pub Date : 2025-09-01 Epub Date: 2025-07-23 DOI: 10.1016/j.engmic.2025.100227

Jingxian Zhang , Peng Xu , Yongjun Wei

引用次数: 0

Tumor-targeting bioluminescent bacteria for in vivo imaging. 肿瘤靶向生物发光细菌在体内成像。

Engineering Microbiology Pub Date : 2025-07-11 eCollection Date: 2025-09-01 DOI: 10.1016/j.engmic.2025.100224

Chenghao Ma, Jingxi Liu, Hongjing Liu, Xiaohan Zhao, Geng Li, Youming Zhang, Tianyu Jiang

引用次数: 0

Carbon sequestration pathways in microorganisms: Advances, strategies, and applications 微生物中的碳固存途径：进展、策略和应用

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-03-08 DOI: 10.1016/j.engmic.2025.100196

Shupeng Ruan , Yuchen Jiang , Aoxue Wang , Xinying Zhang , Ying Lin , Shuli Liang

{"title":"Carbon sequestration pathways in microorganisms: Advances, strategies, and applications","authors":"Shupeng Ruan , Yuchen Jiang , Aoxue Wang , Xinying Zhang , Ying Lin , Shuli Liang","doi":"10.1016/j.engmic.2025.100196","DOIUrl":"10.1016/j.engmic.2025.100196","url":null,"abstract":"<div><div>In recent years, industrial activities have significantly increased atmospheric CO<sub>2</sub> levels, exacerbating global warming. Carbon reduction involves implementing measures to minimize CO<sub>2</sub> emissions from human activities and achieve a balance between carbon absorption and emissions. Therefore, effective reduction of CO<sub>2</sub> emissions is crucial. Conventional physical and chemical methods for CO₂ fixation frequently cause secondary environmental pollution. As a result, utilizing microorganisms for CO<sub>2</sub> fixation has gained considerable interest. This review provides an overview of the natural pathways for microbial CO<sub>2</sub> fixation, recent advancements in artificial CO<sub>2</sub> fixation, and strategies for enhancing the efficiency of microbial CO<sub>2</sub> fixation. We also discuss the conversion of CO<sub>2</sub> into diverse metabolic products and high-value chemicals. By identifying efficient carbon fixation pathways for microorganisms, this review aims to lay the foundation for the biological production of high-value chemicals using CO<sub>2</sub> as a raw material.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100196"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica 脂溶耶氏菌基因编辑CRISPR/Cas9系统的优化

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-03-18 DOI: 10.1016/j.engmic.2025.100193

Jianhui Liu , Yamin Zhu , Jin Hou

{"title":"Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica","authors":"Jianhui Liu , Yamin Zhu , Jin Hou","doi":"10.1016/j.engmic.2025.100193","DOIUrl":"10.1016/j.engmic.2025.100193","url":null,"abstract":"<div><div><em>Yarrowia lipolytica</em> is a promising host for producing valuable chemicals owing to its robustness and metabolic versatility. Efficient genome editing tools are essential for advancing its biotechnological applications. Although CRISPR/Cas9 technology has been applied in <em>Y. lipolytica</em>, achieving a consistently high editing performance remains challenging owing to the low homologous recombination efficiency and variability in system components. In this study, we optimized CRISPR/Cas9-mediated genome editing in <em>Y. lipolytica</em> to enhance its editing efficiency. Using the RNA polymerase III promoter <em>SCR1-tRNA</em> for sgRNA expression, we achieved a gene disruption efficiency of 92.5 %. The tRNA-sgRNA architecture enabled a dual gene disruption efficiency of 57.5 %. <em>KU70</em> deletion in the Cas9 system increased the integration efficiency to 92.5 %, and <em>Rad52</em> and <em>Sae2</em> overexpression boosted homologous recombination. The introduction of Cas9<sup>D147Y, P411T</sup> (iCas9) enhanced the efficiency of both gene disruption and genome integration. This study provides a powerful tool for efficient gene editing in <em>Y. lipolytica</em>, which will accelerate the construction of yeast cell factories.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100193"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional heterologous expression of the reversible Cu-decarboxylase from the lichen, Cladonia uncialis 地衣中可逆cu脱羧酶的功能异源表达

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-05-25 DOI: 10.1016/j.engmic.2025.100211

Harman Gill, John L. Sorensen

{"title":"Functional heterologous expression of the reversible Cu-decarboxylase from the lichen, Cladonia uncialis","authors":"Harman Gill, John L. Sorensen","doi":"10.1016/j.engmic.2025.100211","DOIUrl":"10.1016/j.engmic.2025.100211","url":null,"abstract":"<div><div>Despite the isolation of over 1000 known bioactive lichen mycobiont-derived secondary metabolites (SMs), understanding the genetic basis of their biosynthesis remains elusive. Biosynthetic gene clusters (BGCs) have been tentatively linked to chemical structures, with core genes such as polyketide synthases (PKSs) surrounded by accessory genes like decarboxylases. In this study, we focused on a decarboxylase gene from the genome of the lichen <em>cladonia uncialis</em> (named as <em>Cu</em>-decarboxylase) to elucidate its role in SM biosynthesis. A 963 bp gene was cloned from <em>C. uncialis</em> and expressed in <em>Escherichia coli</em> (BL21(DE3) cells using the pQE80L expression vector. The resulting 35 kDa protein was purified by applying a Ni<sup>+</sup>-NTA column using an FPLC system. Functional activity assays revealed the decarboxylation and reversible carboxylation of resorcinol to 2,4-dihydroxybenzoic acid and orcinol to orsellinic acid. This suggests a potential role for this <em>Cu</em>-decarboxylase in SM biosynthesis.</div><div>Furthermore, the lack of activity on substrates like anthranilic acid and aniline highlighted the importance of the phenolic OH group in facilitating these reactions. The 3D protein structure was predicted with AlphaFold3, based on sequence similarity with a known decarboxylases and revealed the importance of a zinc cofactor for the catalytic activity of the enzyme. The optimization of the reaction conditions, particularly for orsellinic acid production from orcinol, may enhance conversion rates and offer a viable route for industrial-scale production of bioactive compounds. This study marks the first known instance of functional heterologous expression of a non-codon-optimized gene isolated from lichen in <em>E. coli</em>.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100211"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering thermotolerant microbial strains via TrRCC1 overexpression for efficient bioethanol production 通过TrRCC1过表达工程耐热微生物菌株高效生产生物乙醇

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-05-26 DOI: 10.1016/j.engmic.2025.100212

Tingting Chen, Xiao He, Xinyan Zhang, Tian Tian, Jian Cheng, Tingting Long, Yonghao Li

{"title":"Engineering thermotolerant microbial strains via TrRCC1 overexpression for efficient bioethanol production","authors":"Tingting Chen, Xiao He, Xinyan Zhang, Tian Tian, Jian Cheng, Tingting Long, Yonghao Li","doi":"10.1016/j.engmic.2025.100212","DOIUrl":"10.1016/j.engmic.2025.100212","url":null,"abstract":"<div><div>Efficient conversion of corn stover to bioethanol via simultaneous saccharification and fermentation (SSF) is a promising strategy for sustainable biofuel production. A major current barrier to this process is the limited thermotolerance of <em>Saccharomyces cerevisiae</em>, which hampers its performance under the high-temperature conditions required for efficient SSF. In this study, we identified <em>TrRCC1</em>, a gene from <em>Trichoderma reesei</em>, as a candidate for improving microbial stress resistance. Overexpression of <em>TrRCC1</em> in both <em>T. reesei</em> Rut C30 and <em>S. cerevisiae</em> BY4741 significantly enhanced thermotolerance. In <em>T. reesei</em> Rut C30, <em>TrRCC1</em> overexpression improved heat resistance and increased cellulase production by 2.5-fold compared to the wild-type strain. In <em>S. cerevisiae</em> BY4741, <em>TrRCC1</em> overexpression resulted in enhanced thermotolerance and a 21.8 % increase in ethanol production during SSF of corn stover. The ethanol concentration achieved in the SSF process with <em>TrRCC1</em>-overexpressing <em>S. cerevisiae</em> was 44.1 g/L, which was a notable improvement over control strain production. These findings highlight the potential of <em>TrRCC1</em> as a key gene for engineering microbial strains with improved stress resistance to enhance the efficiency of bioethanol production from lignocellulosic biomass.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100212"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to “Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines” [Engineering Microbiology 4 (2024) 100162] “蛋白质热稳定性的调控及其在热减毒疫苗开发中的潜在应用”的勘误[工程微生物学4 (2024)100162]

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-03-25 DOI: 10.1016/j.engmic.2025.100204

Maofeng Wang , Cancan Wu , Nan Liu , Xiaoqiong Jiang , Hongjie Dong , Shubao Zhao , Chaonan Li , Sujuan Xu , Lichuan Gu

引用次数: 0

Superoxide-mediated O2 activation drives radical cyclization in ergot alkaloid biosynthesis 超氧化物介导的O2活化驱动麦角生物碱生物合成中的自由基环化

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-05-08 DOI: 10.1016/j.engmic.2025.100207

Yuanyuan Jiang , Zhong Li , Shengying Li

引用次数: 0

Advances in synthetic microbial ecosystems approach for studying ecological interactions and their influencing factors 合成微生物生态系统方法研究生态相互作用及其影响因素的进展

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1016/j.engmic.2025.100205

Wei Jiang , Sumeng Wang , Fei Gu , Xiaoya Yang , Qingsheng Qi , Quanfeng Liang

{"title":"Advances in synthetic microbial ecosystems approach for studying ecological interactions and their influencing factors","authors":"Wei Jiang , Sumeng Wang , Fei Gu , Xiaoya Yang , Qingsheng Qi , Quanfeng Liang","doi":"10.1016/j.engmic.2025.100205","DOIUrl":"10.1016/j.engmic.2025.100205","url":null,"abstract":"<div><div>Investigating ecological interactions within microbial ecosystems is essential for enhancing our comprehension of key ecological issues, such as community stability, keystone species identification, and the manipulation of community structures. However, exploring these interactions proves challenging within complex natural ecosystems. With advances in synthetic biology, the design of synthetic microbial ecosystems has received increasing attention due to their reduced complexity and enhanced controllability. Various ecological relationships, including commensalism, amensalism, mutualism, competition, and predation have been established within synthetic ecosystems. These relationships are often context-dependent and shaped by physical and chemical environmental factors, as well as by interacting populations and surrounding species. This review consolidates current knowledge of synthetic microbial ecosystems and factors influencing their ecological dynamics. A deeper understanding of how these ecosystems function and respond to different variables will advance our understanding of microbial-community interactions.</div></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":"5 2","pages":"Article 100205"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis 五烯内酯生物合成中细胞色素p450催化烯丙基氧化五烯烯生成1-脱氧五烯酸

Engineering Microbiology Pub Date : 2025-06-01 Epub Date: 2025-04-05 DOI: 10.1016/j.engmic.2025.100206

Jing Li , Chengde Zhang , Shiwen Wu , Jiao Xue , Ke Chen , Zixin Deng , Dongqing Zhu

引用次数: 0