Wei-feng Hu, Yan Wang, Xiao-ran Yue, Wei-wei Xue, Wei Hu, Xin-jing Yue, Yue-Zhong Li
{"title":"具有增强生长特性的升级版黄腐霉菌底盘，可用于高效遗传操作","authors":"Wei-feng Hu, Yan Wang, Xiao-ran Yue, Wei-wei Xue, Wei Hu, Xin-jing Yue, Yue-Zhong Li","doi":"10.1016/j.engmic.2024.100155","DOIUrl":null,"url":null,"abstract":"<div><p>Myxobacteria are well known for multicellular social behaviors and valued for biosynthesis of natural products. Myxobacteria social behaviors such as clumping growth severely hamper strain cultivation and genetic manipulation. Using <em>Myxococcus xanthus</em> DK1622, we engineered Hu04, which is deficient in multicellular behavior and pigmentation. Hu04, while maintaining nutritional growth and a similar metabolic background, exhibits improved dispersed growth, streamlining operational procedures. It achieves high cell densities in culture and is promising for synthetic biology applications.</p></div>","PeriodicalId":100478,"journal":{"name":"Engineering Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667370324000183/pdfft?md5=c2064724dd08cca2123f11fd56275bb0&pid=1-s2.0-S2667370324000183-main.pdf","citationCount":"0","resultStr":"{\"title\":\"An upgraded Myxococcus xanthus chassis with enhanced growth characteristics for efficient genetic manipulation\",\"authors\":\"Wei-feng Hu, Yan Wang, Xiao-ran Yue, Wei-wei Xue, Wei Hu, Xin-jing Yue, Yue-Zhong Li\",\"doi\":\"10.1016/j.engmic.2024.100155\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Myxobacteria are well known for multicellular social behaviors and valued for biosynthesis of natural products. Myxobacteria social behaviors such as clumping growth severely hamper strain cultivation and genetic manipulation. Using <em>Myxococcus xanthus</em> DK1622, we engineered Hu04, which is deficient in multicellular behavior and pigmentation. Hu04, while maintaining nutritional growth and a similar metabolic background, exhibits improved dispersed growth, streamlining operational procedures. It achieves high cell densities in culture and is promising for synthetic biology applications.</p></div>\",\"PeriodicalId\":100478,\"journal\":{\"name\":\"Engineering Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667370324000183/pdfft?md5=c2064724dd08cca2123f11fd56275bb0&pid=1-s2.0-S2667370324000183-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Engineering Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667370324000183\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Engineering Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667370324000183","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
An upgraded Myxococcus xanthus chassis with enhanced growth characteristics for efficient genetic manipulation
Myxobacteria are well known for multicellular social behaviors and valued for biosynthesis of natural products. Myxobacteria social behaviors such as clumping growth severely hamper strain cultivation and genetic manipulation. Using Myxococcus xanthus DK1622, we engineered Hu04, which is deficient in multicellular behavior and pigmentation. Hu04, while maintaining nutritional growth and a similar metabolic background, exhibits improved dispersed growth, streamlining operational procedures. It achieves high cell densities in culture and is promising for synthetic biology applications.
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