Carbohydrate Polymer Technologies and Applications最新文献

{"title":"Wastewater treatment using cellulose nanofiber (CNF)-based green filters: A sustainable approach to water purification","authors":"M.M. Alam ,&nbsp;Md. Johurul Islam ,&nbsp;Sumsun Naher ,&nbsp;Kenneth T V Grattan ,&nbsp;S. Narjim ,&nbsp;Claire Heffernan","doi":"10.1016/j.carpta.2025.100824","DOIUrl":"10.1016/j.carpta.2025.100824","url":null,"abstract":"<div><div>Nanocellulose (NC), as a material, is attracting considerable attention due to its renewable nature, anisotropic shape, excellent mechanical properties, good biocompatibility, tailorable surface chemistry and interesting optical properties. In addition is it easy to source in many developing countries and is inexpensive, as it is often simply considered as a waste product from banana production. In this study, banana fibers (BFs) are chemically modified and converted into Cellulose NanoFibers (CNFs), for incorporation into water filters. The properties of the CNFs thus created were then characterized by using several analytical techniques, such as scanning electron microscopy (SEM), Fourier Transform Infra-Red (FTIR) spectroscopy, X-ray diffraction (XRD) and a thermal study, to understand them better and thus optimize their properties. The aim in so doing is for the CNFs to be used as the basis for fabricating an innovative and inexpensive design of filter, for water purification, and which can be used locally. The key purpose of the work has been the development of a CNF-based water filter, which was fabricated by using a mixture of locally-sourced kaolin (Bijoypur clay) and CNFs, in a 10:1 wt ratio. The water filters thus fabricated were tested and their performance evaluated by exposure to simulated wastewater, containing a combination of six readily available antibiotics (Ciprofloxacin, Clarithromycin, Erythromycin, Metronidazole, Sulfamethoxazole and Trimethoprim). This evaluation was enabled by using UV–Visible spectroscopy measurements, before and after filtration. These simple, CNF-based water filters were found to be highly effective in removing the antibiotic solution used on each pass through the filter, showing a figure of merit of 2.7 ± 0.1 per pass. This approach to filter production provides a simple, inexpensive environmentally friendly and locally-sourced solution to the reduction of antibiotics in wastewater, reducing the pollution problems those antibiotics entering the wastewater supply causes.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100824"},"PeriodicalIF":6.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteolin stabilized in nanosheet and cubic γ-cyclodextrin-based metal organic framework for enhanced bioavailability and anti-inflammatory therapy","authors":"Mubarak G. Bello ,&nbsp;Shiyu Huang ,&nbsp;Zifan Qiao ,&nbsp;Zhenhua Chen ,&nbsp;Lihua Chen","doi":"10.1016/j.carpta.2025.100833","DOIUrl":"10.1016/j.carpta.2025.100833","url":null,"abstract":"<div><div>Luteolin (LT), a biologically active flavonoid with potent anti-inflammatory properties, faces significant limitations due to its instability, poor absorption, and low bioavailability. To overcome these challenges, this study explores the encapsulation of LT within nanosheet and cubic γ-cyclodextrin-based metal-organic frameworks (N-MOF and CD-MOF). This approach achieved a two-fold improvement in LT loading efficiency compared to previously reported methods. A critical finding was the identification of pH as a key factor in optimizing the loading process, which significantly enhanced encapsulation efficiency. The resulting LT/N-MOF and LT/CD-MOF formulations demonstrated improved photo-stability and thermal stability, alongside significantly enhanced cellular permeation. In vitro studies revealed remarkable anti-inflammatory properties of these formulations. Moreover, the bioavailability of LT was increased by 6-fold compared to pure luteolin. In a rat paw edema model, the optimized LT/CD-MOF and LT/N-MOF formulations exhibited 4.86- and 5.4-fold improvements in anti-inflammatory activity, respectively, over pure luteolin. These results underscore the potential of CD-MOFs as effective delivery systems for enhancing the therapeutic efficacy of luteolin.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100833"},"PeriodicalIF":6.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printing-assisted nanoimprinting of hyaluronic acid/chitosan interpolymer complex hydrogels for guiding cell growth","authors":"Young-Qi Xu ,&nbsp;Peng-Chin Tsai ,&nbsp;Kun-Liang Hsieh ,&nbsp;Ming-Chia Li","doi":"10.1016/j.carpta.2025.100829","DOIUrl":"10.1016/j.carpta.2025.100829","url":null,"abstract":"<div><div>The synergy of hydrogels and rapid prototyping technologies offers potential in tissue engineering scaffolds. Hydrogels are promising for soft and hard tissue regeneration but face challenges in shaping and maintaining geometric integrity due to weak mechanical strength during post-swelling. This study develops an interpolymer complex (IPC) hydrogel using HA/CHI polysaccharides for bioengineered skin equivalents, integrating 3D printing and nanoimprinting technologies. The IPC hydrogel is printed on commercial CD and DVD discs, which serve as stampers to create grooved topographies. Scanning Electron Microscopy (SEM) observations reveal that the application of 3D printing-assisted nanoimprinting techniques to IPC hydrogels facilitates large-scale and uniform grooved surface topographies. This design, using the lotus effect to minimize swelling, preserves the nanoimprinted topography during cell culture. Furthermore, our results showed that the grooved topography can guide mouse fibroblasts (mFB), human foreskin (BJ), and human smooth muscle (SMC) cells to directional growth on the IPC membrane with groove structures. Our experimental findings during the wound healing process indicate that an IPC membrane with a CD grooved surface is more conducive to cell proliferation and is more effective as a dressing for wound healing applications.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100829"},"PeriodicalIF":6.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the effectiveness of anticancer properties of capecitabine using aminocaproic acid-hyaluronic acid/sulfonamide IR-MOF nanoplatform for colon cancer treatment","authors":"Sedigheh Alavinia ,&nbsp;Ramin Ghorbani-Vaghei ,&nbsp;Rasool Haddadi ,&nbsp;Khashayar Sanemar ,&nbsp;Seyed Sepehr Uroomiye ,&nbsp;Alireza Nourian","doi":"10.1016/j.carpta.2025.100827","DOIUrl":"10.1016/j.carpta.2025.100827","url":null,"abstract":"<div><div>Colorectal cancer (CRC) is one of the most prevalent and deadly malignancies worldwide, accounting for a significant proportion of cancer-related mortality. Despite advancements in treatment modalities, tumor recurrence and the development of metastases remain major challenges in CRC management. Current chemotherapeutic agents, such as capecitabine (CAP), are widely used in CRC treatment. However, their clinical efficacy is often limited by poor bioavailability and systemic toxicity, which can compromise patient outcomes. Therefore, it is important to find novel ways to prevent these limitations. In this study, we developed a novel nanoscale Zn-sulfonamide isoreticular metal-organic framework (Sul-IRMOF) modified with 6-aminocaproic acid-hyaluronic acid (Sul-IRMOF-ACA-HA) to improve drug release behavior. The MTT assay demonstrated that Sul-IRMOF-ACA-HA@CAP exhibited a 3.2-fold increase in cytotoxicity against CT26 colon cancer cells compared to pure CAP (<em>p</em> ≤ 0.05) after 72 h of treatment. Flow cytometry analysis revealed a 1.52-fold increase in apoptosis induction in CT26 cells treated with Sul-IRMOF-ACA-HA@CAP compared to pure CAP. Tumor weight studies showed that Sul-IRMOF-ACA-HA@CAP reduced tumor weight by 80.3 % compared to the control group (<em>p</em> ≤ 0.05), while CAP treatment achieved only a 25.79 % reduction. Histological assay results are consistent with MTT assay and flow cytometry analysis. The formulation also demonstrated a high entrapment efficiency of 97 % and a sustained release profile in a pH-dependent manner. Furthermore, biochemical analysis of tumors revealed a significant reduction in tumor markers (e.g., SOD, GSH, CAT, and total thiol) in the Sul-IRMOF-ACA-HA@CAP group compared to CAP alone (<em>p</em> ≤ 0.05). This formulation combines the unique properties of HA for receptor-mediated targeting, ACA for enhanced stability, and a Sul-IRMOF for controlled release. The significance of this study lies in its potential to overcome the limitations of conventional chemotherapy by providing a smart, targeted, and pH-responsive drug delivery system that enhances therapeutic efficacy and minimizes systemic toxicity. The combination of HA, ACA, and Sul-IRMOF represents a significant advancement in the field of nanotechnology-based cancer therapy. As a result, Sul-IRMOF-ACA-HA has proven to be effective anticancer formulation and overcomes the issues associated with free CAP.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100827"},"PeriodicalIF":6.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation and bioactivity analysis of exopolysaccharides from Lactiplantibacillus plantarum L3","authors":"Jingyan Zhang,&nbsp;Tao Hu,&nbsp;Yuying Ma,&nbsp;Yiming Ma,&nbsp;Qingshen Sun","doi":"10.1016/j.carpta.2025.100830","DOIUrl":"10.1016/j.carpta.2025.100830","url":null,"abstract":"<div><div>This study systematically investigated the structural characteristics and functional properties of exopolysaccharides (EPS) produced by <em>Lactiplantibacillus plantarum</em> L₃, focusing on their immunomodulatory and antioxidant potential. The EPS, with a molecular weight (Mw) of 79.93 kDa, was composed of diverse monosaccharides, including <span>d</span>-glucose, <span>d</span>-glucosamine, <span>d</span>-mannose, <span>d</span>-galactose, <span>l</span>-rhamnose, <span>d</span>-xylose, <span>d</span>-glucuronic acid, <span>d</span>-galacturonic acid, <span>l</span>-fucose, <span>l</span>-guluronic acid, <span>d</span>-mannuronic acid, and <span>d</span>-ribose, in molar ratios of 397.403: 236.233: 148.526: 52.448: 46.485: 25.344: 6.902: 4.290: 2.158: 1.533: 1.117:0.619: 0.545. Structural analyses using FT-IR, Raman and NMR revealed the presence of both α- and β-glycosidic bonds, while X-ray diffraction indicated an amorphous structure. The Congo red assay confirmed a triple-helix conformation, and scanning electrical microscopy (SEM) and atomic force microscopy (AFM) imaging demonstrated a highly branched and rough surface morphology. Thermal analysis revealed EPS decomposition at 216.33 °C and 260.45 °C, indicating good thermal stability. In vitro experiments using RAW264.7 cells demonstrated that EPS (200–400 µg/mL) significantly enhanced nitric oxide (NO) production and pro-inflammatory cytokine secretion (TNF-α and IL-6), while also increasing the activities of antioxidant enzymes (SOD, CAT, and GSH-Px), highlighting its potent antioxidant capacity. At a higher concentration (600 µg/mL), EPS exhibited anti-inflammatory effects by promoting IL-10 secretion, suggesting a balanced immunomodulatory function. These findings underscore the potential of <em>L. plantarum</em> L₃ EPS as a promising postbiotic agent with dual antioxidant and immunomodulatory properties.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100830"},"PeriodicalIF":6.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan, alginate, and carboxymethyl cellulose-based film for a controlled release of indocyanine green for antibiofilm applications","authors":"Veronica Ciaramitaro ,&nbsp;Magdalena Szpunar ,&nbsp;Filippo Vitale ,&nbsp;Enrico Tornatore ,&nbsp;Alessandro Presentato ,&nbsp;Rosa Alduina ,&nbsp;David Aebisher ,&nbsp;Delia Francesca Chillura Martino ,&nbsp;Andrzej Wal ,&nbsp;Elena Piacenza","doi":"10.1016/j.carpta.2025.100828","DOIUrl":"10.1016/j.carpta.2025.100828","url":null,"abstract":"<div><div>Biofilms on medical devices and wounds significantly contribute to chronic infections in healthcare, as they are highly resistant and difficult to remove, endangering human life and health.</div><div>Searching for a suitable system to counteract this issue, here we enriched a chitosan (CS), sodium alginate (SA), and carboxymethylcellulose (CMC) film with the indocyanine green (ICG) dye as an antibiofilm drug delivery system. These films retained their structural integrity, showing a homogenous dye distribution. Modulating the ICG concentration enabled obtaining different aggregates, whose stability and release kinetics were evaluated in a phosphate-buffered saline (PBS) solution. A portion of ICG remains trapped in the polymer matrix as a local reservoir, and its release was concentration-dependent, with lower or higher concentrations promoting the monomeric ICG or the preferred J-type aggregate. Infrared spectroscopy elucidated non-covalent interactions between the dye and polysaccharide matrix. X-ray diffraction revealed that low and high ICG concentrations improved film crystallinity, while an intermediate concentration preserved the amorphous structure. The dye enhanced film stability by reducing solubility and moisture uptake. We also modeled the ICG release, which followed Higuchi’s diffusion-controlled model, with increasing concentrations enhancing aggregate diffusion. Lastly, ICG-enriched films effectively inhibited <em>Staphylococcus aureus</em> biofilm formation, demonstrating their potential as antimicrobial coatings.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100828"},"PeriodicalIF":6.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes","authors":"Domonkos Pál ,&nbsp;Fanni Bugyi ,&nbsp;Dávid Virág ,&nbsp;Dániel Szabó ,&nbsp;Gitta Schlosser ,&nbsp;Ilona Kovalszky ,&nbsp;Tünde Harkó ,&nbsp;Judit Moldvay ,&nbsp;Lilla Turiák","doi":"10.1016/j.carpta.2025.100826","DOIUrl":"10.1016/j.carpta.2025.100826","url":null,"abstract":"<div><div>Lung cancer is among the most commonly diagnosed cancer types worldwide. Studying the molecular changes underlying lung cancer is crucial for understanding tumor formation and identifying new therapeutic targets and early markers of the disease to decrease mortality. Investigation of glycosaminoglycan chains offer novel insights, as they play major roles in various signaling events in the tumor microenvironment. In this work we have determined the abundance and sulfation characteristics of chondroitin/dermatan sulfate (CS/DS) glycosaminoglycans in human lung adenocarcinoma tissue sections. The cohort included samples with genetic alterations in key genes such as anaplastic lymphoma kinase (<em>ALK)</em>, Kirsten rat sarcoma viral oncogene homolog <em>(KRAS)</em>, epidermal growth factor receptor (<em>EGFR)</em> as well as triple wild type samples. CS/DS chains were cleaved using Chondroitinase ABC enzyme and the disaccharides were extracted from the tissue surface and analyzed using liquid chromatography-mass spectrometry. We identified significant changes in the relative abundance of the 4-<em>O</em>-sulfated CS disaccharide between samples with different genetic alterations and in the 6-<em>O</em>-/4-<em>O</em>-sulfation ratio between the <em>ALK</em> and <em>EGFR</em> group. This is the first study comparing CS/DS characteristics in lung adenocarcinoma samples with different genetic alterations and our findings suggest the importance of further testing to understand their role in tumor progression.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100826"},"PeriodicalIF":6.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of probiotic dark chocolate by alginate-chitosan microcapsules containing protective yeast with potent anti-Salmonella activity","authors":"Sara Shahryari ,&nbsp;Alireza Sadeghi ,&nbsp;Maryam Ebrahimi ,&nbsp;Delasa Rahimi ,&nbsp;Seid Mahdi Jafari","doi":"10.1016/j.carpta.2025.100820","DOIUrl":"10.1016/j.carpta.2025.100820","url":null,"abstract":"<div><div>Application of encapsulated probiotics in complex food matrices is a promising way to improve their viability and targeted delivery. In the present study, sequencing results of the PCR products led to the identification of <em>Pichia kudriavzevii</em> as the selected yeast isolate (YI) with high thermo-osmotolerance ability and low survival under simulated gastrointestinal (SGI) conditions. The inhibitory effect of YI on <em>Salmonella enterica</em> was also significantly (<em>p</em> &lt; 0.05) higher than that of the other foodborne bacteria tested. Furthermore, viable and heat-killed cells of YI, as well as its cell-free supernatant reduced ochratoxin A by 35.28, 59.67 and 24.39 %, respectively according to the results of the HPLC-based assay. Moreover, field emission scanning electron microscopy and Fourier transform infrared analyses approved proper encapsulation of YI in alginate-chitosan (Alg-Ch). Survivability of encapsulated yeast under SGI conditions, before and after application in the produced dark chocolate was significantly higher than the free cells. <em>In situ</em> inhibitory activity of encapsulated yeast against <em>S. enterica</em> was also verified compared to the free yeast and free Ch during storage period of the product. Accordingly, the encapsulated yeast can be used as a proper biological preservative in processing of clean-label PRO chocolate.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100820"},"PeriodicalIF":6.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot production of Carboxymethyl Holocelluloses from mango and pineapple wastes optimized via design of experiments","authors":"Elaine C. de Souza ,&nbsp;Heloise O.M.A. Moura ,&nbsp;Aisha V.S. Pereira ,&nbsp;Jordanna L.B. Costa ,&nbsp;Enrique Rodríguez-Castellón ,&nbsp;Daniel Ballesteros-Plata ,&nbsp;Luciene S. de Carvalho","doi":"10.1016/j.carpta.2025.100822","DOIUrl":"10.1016/j.carpta.2025.100822","url":null,"abstract":"<div><div>The polysaccharide fraction of lignocellulosic biomass represents a promising resource for producing high value-added carbohydrate derivatives. This study aimed to develop a fast and sustainable one-pot process for synthesizing carboxymethyl holocellulose (CMHC) from pineapple crown (PACel) and mango seed tegument (MACel) abundant agro-food wastes, eliminating the need for polysaccharide separation. Holocellulose (cellulose and hemicellulose) was efficiently extracted using a mild acid/peroxide-alkali (APA) treatment, which preserved hemicellulose and generated highly reactive cellulose fractions detected by XRD. Carboxymethylation conditions were screened using a <span>d</span>-Optimal design of experiments, and the process was optimized via response surface methodology (RSM). Degree of substitution reached up to 1.63, with mass yields of up to 246.9% (w/w), surpassing literature values. Statistical models showed high accuracy (<em>F</em> &gt; 36.36, p 〈 0.0001, R² 〉 0.9655). The proposed method offers higher reactivity, lower reagent consumption, and reduced processing time (2:15 h), demonstrating the feasibility of converting agro-waste holocelluloses into high quality water-soluble CMHC products. This research contributes to the efficient valorization of underutilized bioresources in an innovative, rapid, scalable and more environmentally friendly biorefinery process, producing a viable alternative polymer to commercial CMC, with potential applications in the food, cosmetic, pharmaceutical, and oil drilling fluids industries.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100822"},"PeriodicalIF":6.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Ameliorative effects of Hydroxypropyl Betadex and Betadex Sulfobutyl ether sodium on acute ulcerative colitis induced by DSS in Mice","authors":"Lan Zhang ,&nbsp;Fang Li ,&nbsp;Huanhuan Wang ,&nbsp;Bin Chen ,&nbsp;Yongzhi Hua ,&nbsp;Zhentao An","doi":"10.1016/j.carpta.2025.100825","DOIUrl":"10.1016/j.carpta.2025.100825","url":null,"abstract":"<div><div>Hydroxypropyl Betadex (HPB) and Betadex Sulfobutyl Ether Sodium (SEB) were widely employed as pharmaceutical excipients in oral and injectable formulations. This study investigated their therapeutic potential and underlying mechanisms in ulcerative colitis (UC) treatment. RAW264.7 cell experiments and DSS-induced acute UC mice assays were conducted to determine the effects of HPB and SEB on UC. Mechanistic investigations were conducted through network pharmacology, molecular docking, Caco-2 monolayer transepithelial electrical resistance (TEER) assays, and 16S rRNA sequencing of gut microbiota. Treatment with HPB or SEB reduced pro-inflammatory cytokines (NO, IL-6, and TNF-α) levels to 77.8 %-96.3 % of LPS-induced values in RAW264.7 cells. Compared with the DSS-induced UC model group, both HPB and SEB treatment groups showed significantly reduced levels of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) (<em>P</em> &lt; 0.01, <em>P</em> &lt; 0.001). Network pharmacology predicted them to act on proteins related to inflammation. Molecular docking analysis revealed that HPB and SEB could function as “buffer”, demonstrating a tendency to form stable inclusion complexes with intestinal epithelial disruptors. Co-treatment with either (N-AT + HPB), (AMP + HPB), (N-AT + SEB), or (AMP + SEB) attenuated the TEER decline compared to the N-AT or AMP-only groups (<em>P</em> &lt; 0.05). 16S rRNA sequencing showed that SEB and HPB regulated the disturbed diversity of intestinal flora. SEB and HPB were promising candidates for UC treatment, as they inhibited inflammatory pathways, formed epithelial disruptors inclusion, and regulated intestinal flora, providing valuable insights for the development of UC therapies.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100825"},"PeriodicalIF":6.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}