Biosensors最新文献_第3页

Optimization of Nanowell-Based Label-Free Impedance Biosensor Based on Different Nanowell Structures 基于不同纳米孔结构的无标记纳米孔阻抗生物传感器的优化

Biosensors Pub Date : 2024-09-04 DOI: 10.3390/bios14090426

Ali Fardoost, Hassan Raji, Mehdi Javanmard

{"title":"Optimization of Nanowell-Based Label-Free Impedance Biosensor Based on Different Nanowell Structures","authors":"Ali Fardoost, Hassan Raji, Mehdi Javanmard","doi":"10.3390/bios14090426","DOIUrl":"https://doi.org/10.3390/bios14090426","url":null,"abstract":"Nanowell-based impedance-based label-free biosensors have demonstrated significant advantages in sensitivity, simplicity, and accuracy for detecting cancer biomarkers and macromolecules compared to conventional impedance-based biosensors. Although nanowell arrays have previously been employed for biomarker detection, a notable limitation exists in the photolithography step of their fabrication process, leading to a reduced efficiency rate. Historically, the diameter of these nanowells has been 2 μm. To address this issue, we propose alternative geometries for nanowells that feature larger surface areas while maintaining a similar circumference, thereby enhancing the fabrication efficiency of the biosensors. We investigated three geometries: tube, spiral, and quatrefoil. Impedance measurements of the samples were conducted at 10 min intervals using a lock-in amplifier. The study utilized interleukin-6 (IL-6) antibodies and antigens/proteins at a concentration of 100 nM as the target macromolecules. The results indicated that tube-shaped nanowells exhibited the highest sensitivity for detecting IL-6 protein, with an impedance change of 9.55%. In contrast, the spiral, quatrefoil, and circle geometries showed impedance changes of 0.91%, 0.95%, and 1.62%, respectively. Therefore, the tube-shaped nanowell structure presents a promising alternative to conventional nanowell arrays for future studies, potentially enhancing the efficiency and sensitivity of biosensor fabrication.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing Drug Discovery: The Impact of Distinct Designs and Biosensor Integration in Microfluidics-Based Organ-on-a-Chip Technology 革新药物发现：基于微流控芯片的有机技术中不同设计和生物传感器集成的影响

Biosensors Pub Date : 2024-09-03 DOI: 10.3390/bios14090425

Sheng Yuan, Huipu Yuan, David C. Hay, Huan Hu, Chaochen Wang

{"title":"Revolutionizing Drug Discovery: The Impact of Distinct Designs and Biosensor Integration in Microfluidics-Based Organ-on-a-Chip Technology","authors":"Sheng Yuan, Huipu Yuan, David C. Hay, Huan Hu, Chaochen Wang","doi":"10.3390/bios14090425","DOIUrl":"https://doi.org/10.3390/bios14090425","url":null,"abstract":"Traditional drug development is a long and expensive process with high rates of failure. This has prompted the pharmaceutical industry to seek more efficient drug development frameworks, driving the emergence of organ-on-a-chip (OOC) based on microfluidic technologies. Unlike traditional animal experiments, OOC systems provide a more accurate simulation of human organ microenvironments and physiological responses, therefore offering a cost-effective and efficient platform for biomedical research, particularly in the development of new medicines. Additionally, OOC systems enable quick and real-time analysis, high-throughput experimentation, and automation. These advantages have shown significant promise in enhancing the drug development process. The success of an OOC system hinges on the integration of specific designs, manufacturing techniques, and biosensors to meet the need for integrated multiparameter datasets. This review focuses on the manufacturing, design, sensing systems, and applications of OOC systems, highlighting their design and sensing capabilities, as well as the technical challenges they currently face.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Imaging Methodology in Bacterial Biofilms with a Fluorescent Enzymatic Sensor for pepN Activity 利用荧光酶传感器检测 pepN 活性的细菌生物膜先进成像方法

Biosensors Pub Date : 2024-09-03 DOI: 10.3390/bios14090424

Javier Valverde-Pozo, Jose M. Paredes, María Eugenia García-Rubiño, María Dolores Girón, Rafael Salto, Jose M. Alvarez-Pez, Eva M. Talavera

引用次数: 0

Are Aptamer-Based Biosensors the Future of the Detection of the Human Gut Microbiome?—A Systematic Review and Meta-Analysis 基于色聚体的生物传感器是检测人类肠道微生物组的未来吗？

Biosensors Pub Date : 2024-09-02 DOI: 10.3390/bios14090423

Maria João Moreira, Manuela Pintado, José M. M. M. De Almeida

{"title":"Are Aptamer-Based Biosensors the Future of the Detection of the Human Gut Microbiome?—A Systematic Review and Meta-Analysis","authors":"Maria João Moreira, Manuela Pintado, José M. M. M. De Almeida","doi":"10.3390/bios14090423","DOIUrl":"https://doi.org/10.3390/bios14090423","url":null,"abstract":"The gut microbiome is shaped early in life by dietary and lifestyle factors. Specific compounds in the gut affect the growth of different bacterial species and the production of beneficial or harmful byproducts. Dysbiosis of the gut microbiome has been linked to various diseases resulting from the presence of harmful bacteria and their byproducts. Existing methods for detecting microbial species, such as microscopic observation and molecular biological techniques, are costly, labor-intensive, and require skilled personnel. Biosensors, which integrate a recognition element, transducer, amplifier, signal processor, and display unit, can convert biological events into electronic signals. This review provides a comprehensive and systematic survey of scientific publications from 2018 to June 2024, obtained from ScienceDirect, PubMed, and Scopus databases. The aim was to evaluate the current state-of-the-art and identify knowledge gaps in the application of aptamer biosensors for the determination of gut microbiota. A total of 13 eligible publications were categorized based on the type of study: those using microbial bioreceptors (category 1) and those using aptamer bioreceptors (category 2) for the determination of gut microbiota. Point-of-care biosensors are being developed to monitor changes in metabolites that may lead to disease. They are well-suited for use in the healthcare system and offer an excellent alternative to traditional methods. Aptamers are gaining attention due to their stability, specificity, scalability, reproducibility, low production cost, and low immunogenicity. While there is limited research on using aptamers to detect human gut microbiota, they show promise for providing accurate, robust, and cost-effective diagnostic methods for monitoring the gut microbiome.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in Biosensors for Depression Monitoring—Advancing Personalized Treatment 抑郁症监测生物传感器的最新进展--推进个性化治疗

Biosensors Pub Date : 2024-08-30 DOI: 10.3390/bios14090422

Jiaju Yin, Xinyuan Jia, Haorong Li, Bingchen Zhao, Yi Yang, Tian-Ling Ren

引用次数: 0

Optical Interferometric Device for Rapid and Specific Detection of Biological Cells 用于快速、特异性检测生物细胞的光学干涉仪

Biosensors Pub Date : 2024-08-29 DOI: 10.3390/bios14090421

Sándor Valkai, Dániel Petrovszki, Zsombor Fáskerti, Margaréta Baumgärtner, Brigitta Biczók, Kira Dakos, Kevin Dósa, Berill B. Kirner, Anna E. Kocsis, Krisztina Nagy, István Andó, András Dér

{"title":"Optical Interferometric Device for Rapid and Specific Detection of Biological Cells","authors":"Sándor Valkai, Dániel Petrovszki, Zsombor Fáskerti, Margaréta Baumgärtner, Brigitta Biczók, Kira Dakos, Kevin Dósa, Berill B. Kirner, Anna E. Kocsis, Krisztina Nagy, István Andó, András Dér","doi":"10.3390/bios14090421","DOIUrl":"https://doi.org/10.3390/bios14090421","url":null,"abstract":"Here, we report a rapid and accurate optical method for detecting cells from liquid samples in a label-free manner. The working principle of the method is based on the interference of parts of a conical laser beam, coming from a single-mode optical fiber directly, and reflected from a flat glass surface. The glass is functionalized by antibodies against the cells to be detected from the liquid sample. Cells bound to that surface modify the reflected beam, and hence, change the resulting interference pattern, too. By registering and interpreting the variation in the image, the presence of cells from the sample can be detected. As for a demonstration, cell suspensions from a U937 cell line were used in glass chambers functionalized by antibodies (TMG6-5 (mIgG1)) to which the cells specifically bind. The limit of detection (LOD) of the method was also estimated. This proof-of-concept setup offers a cost-effective and easy-to-use way of rapid and specific detection of any type of cells (including pathogens) from suspensions (e.g., body fluids). The possible portability of the device predicts its applicability as a rapid test in clinical diagnostics.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrocatalysis in MOF Films for Flexible Electrochemical Sensing: A Comprehensive Review 用于柔性电化学传感的 MOF 薄膜中的电催化：全面综述

Biosensors Pub Date : 2024-08-28 DOI: 10.3390/bios14090420

Suyuan Zhang, Min Wang, Xusheng Wang, Jun Song, Xue Yang

{"title":"Electrocatalysis in MOF Films for Flexible Electrochemical Sensing: A Comprehensive Review","authors":"Suyuan Zhang, Min Wang, Xusheng Wang, Jun Song, Xue Yang","doi":"10.3390/bios14090420","DOIUrl":"https://doi.org/10.3390/bios14090420","url":null,"abstract":"Flexible electrochemical sensors can adhere to any bendable surface with conformal contact, enabling continuous data monitoring without compromising the surface’s dynamics. Among various materials that have been explored for flexible electronics, metal–organic frameworks (MOFs) exhibit dynamic responses to physical and chemical signals, offering new opportunities for flexible electrochemical sensing technologies. This review aims to explore the role of electrocatalysis in MOF films specifically designed for flexible electrochemical sensing applications, with a focus on their design, fabrication techniques, and applications. We systematically categorize the design and fabrication techniques used in preparing MOF films, including in situ growth, layer-by-layer assembly, and polymer-assisted strategies. The implications of MOF-based flexible electrochemical sensors are examined in the context of wearable devices, environmental monitoring, and healthcare diagnostics. Future research is anticipated to shift from traditional microcrystalline powder synthesis to MOF thin-film deposition, which is expected to not only enhance the performance of MOFs in flexible electronics but also improve sensing efficiency and reliability, paving the way for more robust and versatile sensor technologies.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Developments in Personal Glucose Meters as Point-of-Care Testing Devices (2020–2024) 作为床旁检测设备的个人血糖仪的近期发展（2020-2024 年）

Biosensors Pub Date : 2024-08-27 DOI: 10.3390/bios14090419

Dan-Ni Yang, Shan Geng, Rong Jing, Hao Zhang

{"title":"Recent Developments in Personal Glucose Meters as Point-of-Care Testing Devices (2020–2024)","authors":"Dan-Ni Yang, Shan Geng, Rong Jing, Hao Zhang","doi":"10.3390/bios14090419","DOIUrl":"https://doi.org/10.3390/bios14090419","url":null,"abstract":"Point-of-care testing (POCT) is a contemporary diagnostic approach characterized by its user-friendly nature, cost efficiency, environmental compatibility, and lack of reliance on professional experts. Therefore, it is widely used in clinical diagnosis and other analytical testing fields to meet the demand for rapid and convenient testing. The application of POCT technology not only improves testing efficiency, but also brings convenience and benefits to the healthcare industry. The personal glucose meter (PGM) is a highly successful commercial POCT tool that has been widely used not only for glucose analysis, but also for non-glucose target detection. In this review, the recent advances from 2020 to 2024 in non-glucose target analysis for PGMs as POCT devices are summarized. The signal transduction strategies for non-glucose target analysis based on PGMs, including enzymatic transduction, nanocarrier transduction (enzyme or glucose), and glucose consumption transduction are briefly introduced. Meanwhile, the applications of PGMs in non-glucose target analysis are outlined, encompassing biomedical, environmental, and food analysis, along with other diverse applications. Finally, the prospects of and obstacles to employing PGMs as POCT tools for non-glucose target analysis are discussed.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming Limited Access to Virus Infection Rapid Testing: Development of a Lateral Flow Test for SARS-Cov-2 with Locally Available Resources 克服病毒感染快速检测的局限性：利用当地现有资源开发 SARS-Cov-2 侧流检测试剂盒

Biosensors Pub Date : 2024-08-27 DOI: 10.3390/bios14090416

Estefanía S. Peri Ibáñez, Agostina Mazzeo, Carolina Silva, Maria Juliana Juncos, Guadalupe S. Costa Navarro, Horacio M. Pallarés, Virginia J. Wolos, Gabriel L. Fiszman, Silvia L. Mundo, Julio J. Caramelo, Marcelo J. Yanovsky, Matías Fingermann, Alejandro A. Castello, Andrea V. Gamarnik, Ana S. Peinetti, Daiana A. Capdevila

{"title":"Overcoming Limited Access to Virus Infection Rapid Testing: Development of a Lateral Flow Test for SARS-Cov-2 with Locally Available Resources","authors":"Estefanía S. Peri Ibáñez, Agostina Mazzeo, Carolina Silva, Maria Juliana Juncos, Guadalupe S. Costa Navarro, Horacio M. Pallarés, Virginia J. Wolos, Gabriel L. Fiszman, Silvia L. Mundo, Julio J. Caramelo, Marcelo J. Yanovsky, Matías Fingermann, Alejandro A. Castello, Andrea V. Gamarnik, Ana S. Peinetti, Daiana A. Capdevila","doi":"10.3390/bios14090416","DOIUrl":"https://doi.org/10.3390/bios14090416","url":null,"abstract":"The COVID-19 pandemic highlighted testing inequities in developing countries. Lack of lateral flow test (LFT) manufacturing capacity was a major COVID-19 response bottleneck in low- and middle-income regions. Here we report the development of an open-access LFT for SARS-CoV-2 detection comparable to commercial tests that requires only locally available supplies. The main critical resource is a locally developed horse polyclonal antibody (pAb) whose sensitivity and selectivity are greatly enhanced by affinity purification. We demonstrate that these Abs can perform similarly to commercial monoclonal antibodies (mAbs), as well as mAbs and other pAbs developed against the same antigen. We report a workflow for test optimization using nasopharyngeal swabs collected for RT-qPCR, spiked with the inactivated virus to determine analytical performance characteristics as the limit of detection, among others. Our final prototype showed a performance similar to available tests (sensitivity of 83.3% compared to RT-qPCR, and 90.9% compared to commercial antigen tests). Finally, we discuss the possibility and the challenges of utilizing affinity-purified pAbs as an alternative for the local development of antigen tests in an outbreak context and as a tool to address inequalities in access to rapid tests.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Natural-Polymer-Based Hydrogels for Body Movement and Biomedical Monitoring 用于人体运动和生物医学监测的天然聚合物水凝胶的最新进展

Biosensors Pub Date : 2024-08-27 DOI: 10.3390/bios14090415

Jing Liu, Saisai Li, Shuoze Li, Jinyue Tian, Hang Li, Zhifeng Pan, Lijun Lu, Yanchao Mao

引用次数: 0