Biosensors最新文献_第2页

2PP-Hydrogel Covered Electrodes to Compensate for Media Effects in the Determination of Biomass in a Capillary Wave Micro Bioreactor 在毛细管波微型生物反应器中测定生物量时补偿介质效应的 2PP 水凝胶覆盖电极

Biosensors Pub Date : 2024-09-09 DOI: 10.3390/bios14090438

Sven Meinen, Steffen Brinkmann, Kevin Viebrock, Bassant Elbardisy, Henning Menzel, Rainer Krull, Andreas Dietzel

{"title":"2PP-Hydrogel Covered Electrodes to Compensate for Media Effects in the Determination of Biomass in a Capillary Wave Micro Bioreactor","authors":"Sven Meinen, Steffen Brinkmann, Kevin Viebrock, Bassant Elbardisy, Henning Menzel, Rainer Krull, Andreas Dietzel","doi":"10.3390/bios14090438","DOIUrl":"https://doi.org/10.3390/bios14090438","url":null,"abstract":"Microbioreactors increase information output in biopharmaceutical screening applications because they can be operated in parallel without consuming large quantities of the pharmaceutical formulations being tested. A capillary wave microbioreactor (cwMBR) has recently been reported, allowing cost-efficient parallelization in an array that can be activated for mixing as a whole. Although impedance spectroscopy can directly distinguish between dead and viable cells, the monitoring of cells in suspension within bioreactors is challenging because the signal is influenced by the potentially varying properties of the culture medium. In order to address this challenge, an impedance sensor consisting of two sets of microelectrodes in a cwMBR is presented. Only one set of electrodes was covered by a two-photon cross-linked hydrogel to become insensitive to the influence of cells while remaining sensitive to the culture medium. With this impedance sensor, the biomass of Saccharomyces cerevisiae could be measured in a range from 1 to 20g/l. In addition, the sensor can compensate for a change in the conductivity of the suspension of 5 to 15mS/cm. Moreover, the two-photon cross-linking of hydroxyethyl starch methacrylate hydrogel, which has been studied in detail, recommends itself for even much broader sensing applications in miniaturized bioreactors and biosensors.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in the Synthesis of 3D Complex Plasmonic Intragap Nanostructures and Their Applications in Surface-Enhanced Raman Scattering 三维复杂等离子体隙内纳米结构的合成及其在表面增强拉曼散射中的应用的最新进展

Biosensors Pub Date : 2024-09-06 DOI: 10.3390/bios14090433

Li Ma, Keyi Zhou, Xinyue Wang, Jiayue Wang, Ruyu Zhao, Yifei Zhang, Fang Cheng

{"title":"Recent Progress in the Synthesis of 3D Complex Plasmonic Intragap Nanostructures and Their Applications in Surface-Enhanced Raman Scattering","authors":"Li Ma, Keyi Zhou, Xinyue Wang, Jiayue Wang, Ruyu Zhao, Yifei Zhang, Fang Cheng","doi":"10.3390/bios14090433","DOIUrl":"https://doi.org/10.3390/bios14090433","url":null,"abstract":"Plasmonic intragap nanostructures (PINs) have garnered intensive attention in Raman-related analysis due to their exceptional ability to enhance light–matter interactions. Although diverse synthetic strategies have been employed to create these nanostructures, the emphasis has largely been on PINs with simple configurations, which often fall short in achieving effective near-field focusing. Three-dimensional (3D) complex PINs, distinguished by their intricate networks of internal gaps and voids, are emerging as superior structures for effective light trapping. These structures facilitate the generation of hot spots and hot zones that are essential for enhanced near-field focusing. Nevertheless, the synthesis techniques for these complex structures and their specific impacts on near-field focusing are not well-documented. This review discusses the recent advancements in the synthesis of 3D complex PINs and their applications in surface-enhanced Raman scattering (SERS). We begin by describing the foundational methods for fabricating simple PINs, followed by a discussion on the rational design strategies aimed at developing 3D complex PINs with superior near-field focusing capabilities. We also evaluate the SERS performance of various 3D complex PINs, emphasizing their advanced sensing capabilities. Lastly, we explore the future perspective of 3D complex PINs in SERS applications.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cost-Effective and Easy-to-Fabricate Conductive Velcro Dry Electrode for Durable and High-Performance Biopotential Acquisition 一种经济高效、易于制造的导电尼龙搭扣干电极，可用于持久、高性能的生物电位采集

Biosensors Pub Date : 2024-09-06 DOI: 10.3390/bios14090432

Jun Guo, Xuanqi Wang, Ruiyu Bai, Zimo Zhang, Huazhen Chen, Kai Xue, Chuang Ma, Dawei Zang, Erwei Yin, Kunpeng Gao, Bowen Ji

{"title":"A Cost-Effective and Easy-to-Fabricate Conductive Velcro Dry Electrode for Durable and High-Performance Biopotential Acquisition","authors":"Jun Guo, Xuanqi Wang, Ruiyu Bai, Zimo Zhang, Huazhen Chen, Kai Xue, Chuang Ma, Dawei Zang, Erwei Yin, Kunpeng Gao, Bowen Ji","doi":"10.3390/bios14090432","DOIUrl":"https://doi.org/10.3390/bios14090432","url":null,"abstract":"Compared with the traditional gel electrode, the dry electrode is being taken more seriously in bioelectrical recording because of its easy preparation, long-lasting ability, and reusability. However, the commonly used dry AgCl electrodes and silver cloth electrodes are generally hard to record through hair due to their flat contact surface. Claw electrodes can contact skin through hair on the head and body, but the internal claw structure is relatively hard and causes discomfort after being worn for a few hours. Here, we report a conductive Velcro electrode (CVE) with an elastic hook hair structure, which can collect biopotential through body hair. The elastic hooks greatly reduce discomfort after long-time wearing and can even be worn all day. The CVE electrode is fabricated by one-step immersion in conductive silver paste based on the cost-effective commercial Velcro, forming a uniform and durable conductive coating on a cluster of hook microstructures. The electrode shows excellent properties, including low impedance (15.88 kΩ @ 10 Hz), high signal-to-noise ratio (16.0 dB), strong water resistance, and mechanical resistance. After washing in laundry detergent, the impedance of CVE is still 16% lower than the commercial AgCl electrodes. To verify the mechanical strength and recovery capability, we conducted cyclic compression experiments. The results show that the displacement change of the electrode hook hair after 50 compression cycles was still less than 1%. This electrode provides a universal acquisition scheme, including effective acquisition of different parts of the body with or without hair. Finally, the gesture recognition from electromyography (EMG) by the CVE electrode was applied with accuracy above 90%. The CVE proposed in this study has great potential and promise in various human–machine interface (HMI) applications that employ surface biopotential signals on the body or head with hair.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave Technique for Linear Skull Fracture Detection—Simulation and Experimental Study Using Realistic Human Head Models 用于线性颅骨骨折检测的微波技术--利用真实人体头部模型进行的模拟和实验研究

Biosensors Pub Date : 2024-09-06 DOI: 10.3390/bios14090434

Mariella Särestöniemi, Daljeet Singh, Mikael von und zu Fraunberg, Teemu Myllylä

{"title":"Microwave Technique for Linear Skull Fracture Detection—Simulation and Experimental Study Using Realistic Human Head Models","authors":"Mariella Särestöniemi, Daljeet Singh, Mikael von und zu Fraunberg, Teemu Myllylä","doi":"10.3390/bios14090434","DOIUrl":"https://doi.org/10.3390/bios14090434","url":null,"abstract":"Microwave (MW) sensing is regarded as a promising technique for various medical monitoring and diagnostic applications due to its numerous advantages and the potential to be developed into a portable device for use outside hospital settings. The detection of skull fractures and the monitoring of their healing process would greatly benefit from a rapidly and frequently usable application that can be employed outside the hospital. This paper presents a simulation- and experiment-based study on skull fracture detection with the MW technique using realistic models for the first time. It also presents assessments on the most promising frequency ranges for skull fracture detection within the Industrial, Scientific and Medical (ISM) and ultrawideband (UWB) ranges. Evaluations are carried out with electromagnetic simulations using different head tissue layer models corresponding to different locations in the human head, as well as an anatomically realistic human head simulation model. The measurements are conducted with a real human skull combined with tissue phantoms developed in our laboratory. The comprehensive evaluations show that fractures cause clear differences in antenna and channel parameters (S11 and S21). The difference in S11 is 0.1–20 dB and in S21 is 0.1–30 dB, depending on the fracture width and location. Skull fractures with a less than 1 mm width can be detected with microwaves at different fracture locations. The detectability is frequency dependent. Power flow representations illustrate how fractures impact on the signal propagation at different frequencies. MW-based detection of skull fractures provides the possibility to (1) detect fractures using a safe and low-cost portable device, (2) monitor the healing-process of fractures, and (3) bring essential information for emerging portable MW-based diagnostic applications that can detect, e.g., strokes.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of QCM and Electrochemical Aptasensors for SARS-CoV-2 Detection 用于检测 SARS-CoV-2 的 QCM 和电化学 Aptasensor 的比较分析

Biosensors Pub Date : 2024-09-06 DOI: 10.3390/bios14090431

Katarína Nemčeková, Jana Korčeková, Veronika Svitková, Denis Baraniak, Michaela Domšicová, Eva Melníková, Michaela Hornychová, Viktória Szebellaiová, Miroslav Gál, Alexandra Poturnayová

{"title":"Comparative Analysis of QCM and Electrochemical Aptasensors for SARS-CoV-2 Detection","authors":"Katarína Nemčeková, Jana Korčeková, Veronika Svitková, Denis Baraniak, Michaela Domšicová, Eva Melníková, Michaela Hornychová, Viktória Szebellaiová, Miroslav Gál, Alexandra Poturnayová","doi":"10.3390/bios14090431","DOIUrl":"https://doi.org/10.3390/bios14090431","url":null,"abstract":"The rapid and accurate detection of SARS-CoV-2, particularly its spike receptor-binding domain (S-RBD), was crucial for managing the COVID-19 pandemic. This study presents the development and optimization of two types of aptasensors: quartz crystal microbalance (QCM) and electrochemical sensors, both employing thiol-modified DNA aptamers for S-RBD detection. The QCM aptasensor demonstrated exceptional sensitivity, achieved by optimizing aptamer concentration, buffer composition, and pre-treatment conditions, with a limit of detection (LOD) of 0.07 pg/mL and a linear range from 1 pg/mL to 0.1 µg/mL, and a significant frequency change was observed upon target binding. The electrochemical aptasensor, designed for rapid and efficient preparation, utilized a one-step modification process that reduced the preparation time to 2 h while maintaining high sensitivity and specificity. Electrochemical impedance spectroscopy (EIS) enabled the detection of S-RBD concentrations as low as 132 ng/mL. Both sensors exhibited high specificity, with negligible non-specific interactions observed in the presence of competing proteins. Additionally, the QCM aptasensor’s functionality and stability were verified in biological fluids, indicating its potential for real-world applications. This study highlights the comparative advantages of QCM and electrochemical aptasensors in terms of preparation time, sensitivity, and specificity, offering valuable insights for the development of rapid, sensitive, and specific diagnostic tools for the detection of SARS-CoV-2 and other viruses.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Advanced Liver-on-a-Chip Model for Evaluating Drug Metabolism and Hepatotoxicity 用于评估药物代谢和肝毒性的先进肝芯片模型

Biosensors Pub Date : 2024-09-06 DOI: 10.3390/bios14090435

Sonia Frojdenfal, Agnieszka Zuchowska

引用次数: 0

Tumor Microenvironment Based on Extracellular Matrix Hydrogels for On-Chip Drug Screening 基于细胞外基质水凝胶的肿瘤微环境用于片上药物筛选

Biosensors Pub Date : 2024-09-05 DOI: 10.3390/bios14090429

Xiaoyan Liu, Jinxiong Cheng, Yingcan Zhao

引用次数: 0

Enhancing Colorimetric Detection of Nucleic Acids on Nitrocellulose Membranes: Cutting-Edge Applications in Diagnostics and Forensics 增强硝酸纤维素膜上核酸的比色检测：诊断和法医学中的前沿应用

Biosensors Pub Date : 2024-09-05 DOI: 10.3390/bios14090430

Nidhi Subhashini, Yannick Kerler, Marcus M. Menger, Olga Böhm, Judith Witte, Christian Stadler, Alexander Griberman

{"title":"Enhancing Colorimetric Detection of Nucleic Acids on Nitrocellulose Membranes: Cutting-Edge Applications in Diagnostics and Forensics","authors":"Nidhi Subhashini, Yannick Kerler, Marcus M. Menger, Olga Böhm, Judith Witte, Christian Stadler, Alexander Griberman","doi":"10.3390/bios14090430","DOIUrl":"https://doi.org/10.3390/bios14090430","url":null,"abstract":"This study re-introduces a protein-free rapid test method for nucleic acids on paper based lateral flow assays utilizing special multichannel nitrocellulose membranes and DNA-Gold conjugates, achieving significantly enhanced sensitivity, easier protocols, reduced time of detection, reduced costs of production and advanced multiplexing possibilities. A protein-free nucleic acid-based lateral flow assay (NALFA) with a limit of detection of 1 pmol of DNA is shown for the first time. The total production duration of such an assay was successfully reduced from the currently known several days to just a few hours. The simplification and acceleration of the protocol make the method more accessible and practical for various applications. The developed method supports multiplexing, enabling the simultaneous detection of up to six DNA targets. This multiplexing capability is a significant improvement over traditional line tests and offers more comprehensive diagnostic potential in a single assay. The approach significantly reduces the run time compared to traditional line tests, which enhances the efficiency of diagnostic procedures. The protein-free aspect of this assay minimizes the prevalent complications of cross-reactivity in immunoassays especially in cases of multiplexing. It is also demonstrated that the NALFA developed in this study is amplification-free and hence does not rely on specialized technicians, nor does it involve labour-intensive steps like DNA extraction and PCR processes. Overall, this study presents a robust, efficient, and highly sensitive platform for DNA or RNA detection, addressing several limitations of current methods documented in the literature. The advancements in sensitivity, cost reduction, production time, and multiplexing capabilities mark a substantial improvement, holding great potential for various applications in diagnostics, forensics, and molecular biology.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Assessment of Methyl Methanesulfonate Absorption by Amphipods from the Environment Using Lux-Biosensors 利用 Lux 生物传感器评估片脚类动物对环境中甲磺酸甲酯的吸收情况

Biosensors Pub Date : 2024-09-05 DOI: 10.3390/bios14090427

Uliana S. Novoyatlova, Anna A. Kudryavtseva, Sergey V. Bazhenov, Anna A. Utkina, Vadim V. Fomin, Shamil A. Nevmyanov, Bagila S. Zhoshibekova, Maria A. Fedyaeva, Mikhail Y. Kolobov, Ilya V. Manukhov

{"title":"The Assessment of Methyl Methanesulfonate Absorption by Amphipods from the Environment Using Lux-Biosensors","authors":"Uliana S. Novoyatlova, Anna A. Kudryavtseva, Sergey V. Bazhenov, Anna A. Utkina, Vadim V. Fomin, Shamil A. Nevmyanov, Bagila S. Zhoshibekova, Maria A. Fedyaeva, Mikhail Y. Kolobov, Ilya V. Manukhov","doi":"10.3390/bios14090427","DOIUrl":"https://doi.org/10.3390/bios14090427","url":null,"abstract":"The ability of aquatic mesofauna representatives involved in trophic chains to sorb and accumulate toxicants is important for understanding the functioning of aquatic ecosystems and for fishing industry. This study investigated the capacity of marine amphipod Gammarus oceanicus and freshwater amphipods Eulimnogammarus vittatus and Gammarus lacustris to absorb the DNA-alkylating agent methyl methanesulfonate (MMS). The presence of alkylating agents in the environment and in the tissues of the amphipods was determined using whole-cell lux-biosensor Escherichia coli MG1655 pAlkA-lux, in which the luxCDABE genes from Photorhabdus luminescens, enabling the luminescence of the cell culture, are controlled by the PalkA promoter of DNA glycosylase. It was shown that within one day of incubation in water containing MMS at a concentration above 10 μM, the amphipods absorbed the toxicant and their tissues produce more alkylation damage to biosensor cells than the surrounding water. Concentrations of MMS above 1 mM in the environment caused the death of the amphipods before the toxicant could be significantly concentrated in their tissues. The sensitivity and the capacity to absorb MMS were found to be approximately the same for the marine amphipod G. oceanicus and the freshwater amphipods E. vittatus and G. lacustris.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Labeling with Mulberrin-Cy3: A New Fluorescent Probe for Live Cell Visualization 用 Mulberrin-Cy3 标记线粒体：用于活细胞可视化的新型荧光探针

Biosensors Pub Date : 2024-09-05 DOI: 10.3390/bios14090428

Gangxiang Yuan, Yiwei Luo, Peng Qian, Ningjia He

{"title":"Mitochondrial Labeling with Mulberrin-Cy3: A New Fluorescent Probe for Live Cell Visualization","authors":"Gangxiang Yuan, Yiwei Luo, Peng Qian, Ningjia He","doi":"10.3390/bios14090428","DOIUrl":"https://doi.org/10.3390/bios14090428","url":null,"abstract":"Mitochondria, crucial intracellular organelles, are central to energy metabolism, signal transduction, apoptosis, calcium homeostasis, and a myriad of other biological processes, making them a focal point in diverse research fields. The capacity to fluorescently label and visually track mitochondria is crucial for understanding their biological roles. We present mulberrin-Cy3, a novel small molecule fluorescent probe that selectively labels mitochondria in animal cells, including cancer cells, with relative ease. This protocol details the synthesis of mulberrin-Cy3 and its use for visualizing mitochondria in living cells. The synthesis is straightforward and time-efficient, and the labeling method is more accessible than traditional approaches, providing a cost-effective option for mitochondrial visualization at room temperature. The labeling is rapid, with effective labeling achieved within 5 min of incubation. The fluorescent signal is stable and brighter, offering a significant advantage over existing methods. Mulberrin-Cy3 represents a promising mitochondrial labeling compound, providing researchers with a novel experimental tool to explore the complex biological functions of mitochondria. This innovation has the potential to significantly advance our comprehension of mitochondrial dynamics and their role in cellular health and disease.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142186550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0