Biosensors最新文献_第10页

Integrated Droplet-Based Digital Loop-Mediated Isothermal Amplification Microfluidic Chip with Droplet Generation, Incubation, and Continuous Fluorescence Detection 具有液滴生成、孵育和连续荧光检测功能的集成液滴式数字环路介导等温扩增微流控芯片

Biosensors Pub Date : 2024-07-08 DOI: 10.3390/bios14070334

Yen-Heng Lin, Yuan-Ting Hung, Wei Chang, Chiuan-Chian Chiou

{"title":"Integrated Droplet-Based Digital Loop-Mediated Isothermal Amplification Microfluidic Chip with Droplet Generation, Incubation, and Continuous Fluorescence Detection","authors":"Yen-Heng Lin, Yuan-Ting Hung, Wei Chang, Chiuan-Chian Chiou","doi":"10.3390/bios14070334","DOIUrl":"https://doi.org/10.3390/bios14070334","url":null,"abstract":"This study integrated sample partition, incubation, and continuous fluorescence detection on a single microfluidic chip for droplet-based digital Loop-Mediated Isothermal Amplification (LAMP) of nucleic acids. This integration eliminated the need to transfer reactions between different platforms, avoiding sample contamination and loss. Prior to the reaction, filling the channels with an oil phase and adding a glass cover slip on top of the chip overcame the problem of bubble generation in the channels during the LAMP reaction due to heating. Additionally, using two fluorescence intensity thresholds enabled simultaneous detection and counting of positive and negative droplets within a single fluorescence detection channel. The chip can partition approximately 6000 droplets from a 5 µL sample within 10 min, with a droplet diameter of around 110 µm and a coefficient of variation (CV) value of 0.82%. Staphylococcus aureus was quantified via the proposed platform. The results demonstrated a highly accurate correlation coefficient (R = 0.9998), and the detection limit reached a concentration of 1.7 × 102 copies/µL. The entire process of the droplet digital LAMP reaction, from droplet generation to incubation to quantitative results, took a maximum of 70 min.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luminescence Probes in Bio-Applications: From Principle to Practice 生物应用中的发光探针：从原理到实践

Biosensors Pub Date : 2024-07-08 DOI: 10.3390/bios14070333

Tao Yan, Fan Weng, Yang Ming, Shijie Zhu, Miao Zhu, Chunsheng Wang, Changfa Guo, Kai Zhu

引用次数: 0

A 3D-Printed Do-It-Yourself ELISA Plate Reader as a Biosensor Tested on TNFα Assay 三维打印的自助式酶联免疫吸附板阅读器作为生物传感器在 TNFα 检测中进行测试

Biosensors Pub Date : 2024-07-06 DOI: 10.3390/bios14070331

Miroslav Pohanka, Ondřej Keresteš, Jitka Žáková

引用次数: 0

Recent Progress in Organic Electrochemical Transistor-Structured Biosensors 有机电化学晶体管结构生物传感器的最新进展

Biosensors Pub Date : 2024-07-04 DOI: 10.3390/bios14070330

Zhuotao Hu, Yingchao Hu, Lu Huang, Wei Zhong, Jianfeng Zhang, Dengyun Lei, Yayi Chen, Yao Ni, Yuan Liu

引用次数: 0

Thiram Determination in Milk Samples by Surface Plasmon Resonance Based on Molecularly Imprinted Polymers and Sulphur-Doped Titanium Dioxide 基于分子印迹聚合物和掺硫二氧化钛的表面等离子体共振法测定牛奶样品中的硫拉姆

Biosensors Pub Date : 2024-07-03 DOI: 10.3390/bios14070329

Sezen Harmankaya, Hacı Ahmet Deveci, Ahmet Harmankaya, Fatma Hazan Gül, Necip Atar, Mehmet Lütfi Yola

{"title":"Thiram Determination in Milk Samples by Surface Plasmon Resonance Based on Molecularly Imprinted Polymers and Sulphur-Doped Titanium Dioxide","authors":"Sezen Harmankaya, Hacı Ahmet Deveci, Ahmet Harmankaya, Fatma Hazan Gül, Necip Atar, Mehmet Lütfi Yola","doi":"10.3390/bios14070329","DOIUrl":"https://doi.org/10.3390/bios14070329","url":null,"abstract":"In this work, a new surface plasmon resonance (SPR) sensor based on sulphur-doped titanium dioxide (S-TiO2) nanostructures and molecularly imprinted polymer (MIP) was presented for thiram (THI) determination in milk samples. Firstly, the S-TiO2 nanomaterial with a high product yield was prepared by using a facile sol–gel hydrolysis technique with a high product yield. After that, UV polymerization was carried out for the preparation of the THI-imprinted SPR chip based on S-TiO2 using a mixture including ethylene glycol dimethacrylate (EGDMA) as the cross-linker, N,N′-azobisisobutyronitrile (AIBN) as the initiator, and methacryloylamidoglutamicacid (MAGA) as the monomer. The reliability of the sensor preparation procedure has been successfully proven by characterization studies of the prepared nanomaterials and SPR chip surfaces through spectroscopic, microscopic, and electrochemical methods. As a result, the prepared SPR sensor showed linearity in the range of 1.0 × 10−9–1.0 × 10−7 M with a detection limit (LOD) of 3.3 × 10−10 M in the real samples, and a sensor technique for THI determination with high sensitivity, repeatability, and selectivity can be included in the literature.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141520693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Au24Cd Nanoenzyme Coating for Enhancing Electrochemical Sensing Performance of Metal Wire Microelectrodes 用于提高金属丝微电极电化学传感性能的 Au24Cd 纳米酶涂层

Biosensors Pub Date : 2024-07-02 DOI: 10.3390/bios14070328

Jia-Yi Chen, Shuang Huang, Shuang-Jie Liu, Zheng-Jie Liu, Xing-Yuan Xu, Meng-Yi He, Chuan-Jie Yao, Tao Zhang, Han-Qi Yang, Xin-Shuo Huang, Jing Liu, Xiao-Dong Zhang, Xi Xie, Hui-Jiuan Chen

{"title":"Au24Cd Nanoenzyme Coating for Enhancing Electrochemical Sensing Performance of Metal Wire Microelectrodes","authors":"Jia-Yi Chen, Shuang Huang, Shuang-Jie Liu, Zheng-Jie Liu, Xing-Yuan Xu, Meng-Yi He, Chuan-Jie Yao, Tao Zhang, Han-Qi Yang, Xin-Shuo Huang, Jing Liu, Xiao-Dong Zhang, Xi Xie, Hui-Jiuan Chen","doi":"10.3390/bios14070328","DOIUrl":"https://doi.org/10.3390/bios14070328","url":null,"abstract":"Dopamine (DA), ascorbic acid (AA), and uric acid (UA) are crucial neurochemicals, and their abnormal levels are involved in various neurological disorders. While electrodes for their detection have been developed, achieving the sensitivity required for in vivo applications remains a challenge. In this study, we proposed a synthetic Au24Cd nanoenzyme (ACNE) that significantly enhanced the electrochemical performance of metal electrodes. ACNE-modified electrodes demonstrated a remarkable 10-fold reduction in impedance compared to silver microelectrodes. Furthermore, we validated their excellent electrocatalytic activity and sensitivity using five electrochemical detection methods, including cyclic voltammetry, differential pulse voltammetry, square-wave pulse voltammetry, normal pulse voltammetry, and linear scanning voltammetry. Importantly, the stability of gold microelectrodes (Au MEs) modified with ACNEs was significantly improved, exhibiting a 30-fold enhancement compared to Au MEs. This improved performance suggests that ACNE functionalization holds great promise for developing micro-biosensors with enhanced sensitivity and stability for detecting small molecules.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141506397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensors for Cancer Biomarkers Based on Mesoporous Silica Nanoparticles 基于介孔二氧化硅纳米颗粒的癌症生物标记物生物传感器

Biosensors Pub Date : 2024-06-30 DOI: 10.3390/bios14070326

Minja Mladenović, Stefan Jarić, Mirjana Mundžić, Aleksandra Pavlović, Ivan Bobrinetskiy, Nikola Ž. Knežević

引用次数: 0

Biosensor with Microchannel for Broadband Dielectric Characterization of Nanoliter Cell Suspensions up to 110 GHz 用于纳升细胞悬浮液宽带介电特性分析的微通道生物传感器，频率高达 110 千兆赫

Biosensors Pub Date : 2024-06-30 DOI: 10.3390/bios14070327

Wen Sun, Jianhua Wang, Jin Chen, Xiwei Huang, Xin Rao, Jiangtao Su, Yuqiao Huang, Boyu Zhang, Lingling Sun

{"title":"Biosensor with Microchannel for Broadband Dielectric Characterization of Nanoliter Cell Suspensions up to 110 GHz","authors":"Wen Sun, Jianhua Wang, Jin Chen, Xiwei Huang, Xin Rao, Jiangtao Su, Yuqiao Huang, Boyu Zhang, Lingling Sun","doi":"10.3390/bios14070327","DOIUrl":"https://doi.org/10.3390/bios14070327","url":null,"abstract":"Cell dielectric property measurement holds significant potential for application in cell detection and diagnosis due to its label-free and noninvasive nature. In this study, we developed a biosensor designed to measure the permittivity of liquid samples, particularly cell suspensions at the nanoliter scale, utilizing microwave and millimeter wave coplanar waveguides in conjunction with a microchannel. This biosensor facilitates the measurement of scattering parameters within a frequency domain ranging from 1 GHz to 110 GHz. The obtained scattering parameters are then converted into dielectric constants using specific algorithms. A cell capture structure within the microchannel ensures that cell suspensions remain stable within the measurement zone. The feasibility of this biosensor was confirmed by comparison with a commercial Keysight probe. We measured the dielectric constants of three different cell suspensions (HepG2, A549, MCF-7) using our biosensor. We also counted the number of cells captured in multiple measurements for each cell type and compared the corresponding changes in permittivity. The results indicated that the real part of the permittivity of HepG2 cells is 0.2–0.8 lower than that of the other two cell types. The difference between A549 and MCF-7 was relatively minor, only 0.2–0.4. The fluctuations in the dielectric spectrum caused by changes in cell numbers during measurements were smaller than the differences observed between different cell types. Thus, the sensor is suitable for measuring cell suspensions and can be utilized for label-free, noninvasive studies in identifying biological cell suspensions.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141520695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Directed Evolution of Protein-Based Sensors for Anaerobic Biological Activation of Methane 基于蛋白质的甲烷厌氧生物活化传感器的定向进化

Biosensors Pub Date : 2024-06-30 DOI: 10.3390/bios14070325

Ehsan Bahrami Moghadam, Nam Nguyen, Yixi Wang, Patrick C. Cirino

{"title":"Directed Evolution of Protein-Based Sensors for Anaerobic Biological Activation of Methane","authors":"Ehsan Bahrami Moghadam, Nam Nguyen, Yixi Wang, Patrick C. Cirino","doi":"10.3390/bios14070325","DOIUrl":"https://doi.org/10.3390/bios14070325","url":null,"abstract":"Microbial alkane degradation pathways provide biological routes for converting these hydrocarbons into higher-value products. We recently reported the functional expression of a methyl-alkylsuccinate synthase (Mas) system in Escherichia coli, allowing for the heterologous anaerobic activation of short-chain alkanes. However, the enzymatic activation of methane via natural or engineered alkylsuccinate synthases has yet to be reported. To address this, we employed high-throughput screening to engineer the itaconate (IA)-responsive regulatory protein ItcR (WT-ItcR) from Yersinia pseudotuberculosis to instead respond to methylsuccinate (MS, the product of methane addition to fumarate), resulting in genetically encoded biosensors for MS. Here, we describe ItcR variants that, when regulating fluorescent protein expression in E. coli, show increased sensitivity, improved overall response, and enhanced specificity toward exogenously added MS relative to the wild-type repressor. Structural modeling and analysis of the ItcR ligand binding pocket provide insights into the altered molecular recognition. In addition to serving as biosensors for screening alkylsuccinate synthases capable of methane activation, MS-responsive ItcR variants also establish a framework for the directed evolution of other molecular reporters, targeting longer-chain alkylsuccinate products or other succinate derivatives.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141506399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Scalable, Programmable Neural Stimulator for Enhancing Generalizability in Neural Interface Applications 用于增强神经接口应用通用性的可扩展、可编程神经刺激器

Biosensors Pub Date : 2024-06-28 DOI: 10.3390/bios14070323

Meng Yin, Xiao Wang, Liuxindai Zhang, Guijun Shu, Zhen Wang, Shoushuang Huang, Ming Yin

{"title":"A Scalable, Programmable Neural Stimulator for Enhancing Generalizability in Neural Interface Applications","authors":"Meng Yin, Xiao Wang, Liuxindai Zhang, Guijun Shu, Zhen Wang, Shoushuang Huang, Ming Yin","doi":"10.3390/bios14070323","DOIUrl":"https://doi.org/10.3390/bios14070323","url":null,"abstract":"Each application of neurostimulators requires unique stimulation parameter specifications to achieve effective stimulation. Balancing the current magnitude with stimulation resolution, waveform, size, and channel count is challenging, leading to a loss of generalizability across broad neural interfaces. To address this, this paper proposes a highly scalable, programmable neurostimulator with a System-on-Chip (SOC) capable of 32 channels of independent stimulation. The compliance voltage reaches up to ±22.5 V. A pair of 8-bit current-mode DACs support independent waveforms for source and sink operations and feature a user-selectable dual range for low-current intraparenchymal microstimulation with a resolution of 4.31 μA/bit, as well as high current stimulation for spinal cord and DBS applications with a resolution of 48.00 μA/bit, achieving a wide stimulation range of 12.24 mA while maintaining high-resolution biological stimulation. A dedicated communication protocol enables full programmable control of stimulation waveforms, effectively improving the range of stimulation parameters. In vivo electrophysiological experiments successfully validate the functionality of the proposed stimulator. This flexible stimulator architecture aims to enhance its generality across a wide range of neural interfaces and will provide more diverse and refined stimulation strategies.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141520696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0