Biosensors最新文献_第9页

Magnetic Immunoassay Based on Au Pt Bimetallic Nanoparticles/Carbon Nanotube Hybrids for Sensitive Detection of Tetracycline Antibiotics 基于金铂双金属纳米粒子/碳纳米管杂化物的磁性免疫测定，用于灵敏检测四环素类抗生素

Biosensors Pub Date : 2024-07-15 DOI: 10.3390/bios14070342

Jianxia Lv, Rui Huang, Kun Zeng, Zhen Zhang

{"title":"Magnetic Immunoassay Based on Au Pt Bimetallic Nanoparticles/Carbon Nanotube Hybrids for Sensitive Detection of Tetracycline Antibiotics","authors":"Jianxia Lv, Rui Huang, Kun Zeng, Zhen Zhang","doi":"10.3390/bios14070342","DOIUrl":"https://doi.org/10.3390/bios14070342","url":null,"abstract":"Misusage of tetracycline (TC) antibiotics residue in animal food has posed a significant threat to human health. Therefore, there is an urgent need to develop highly sensitive and robust assays for detecting TC. In the current study, gold and platinum nanoparticles were deposited on carbon nanotubes (CNTs) through the superposition method (Au@Pt/CNTs-s) and one-pot method (Au@Pt/CNTs-o). Au@Pt/CNTs-s displayed higher enzyme-like activity than Au@Pt/CNTs-o, which were utilized for the development of sensitive magnetic immunoassays. Under the optimized conditions, the limits of detection (LODs) of magnetic immunoassays assisted by Au@Pt/CNTs-s and Au@Pt/CNTs-o against TCs could reach 0.74 ng/mL and 1.74 ng/m, respectively, which were improved 6-fold and 2.5-fold in comparison with conventional magnetic immunoassay. In addition, the measurement of TC-family antibiotics was implemented by this assay, and ascribed to the antibody used that could recognize TC, oxytetracycline, chlortetracycline, and doxycycline with high cross-reactivity. Furthermore, the method showed good accuracy (recoveries, 92.1–114.5% for milk; 88.6–92.4% for pork samples), which also were applied for determination of the targets in real samples. This study provides novel insights into the rapid detection of targets based on high-performance nanocatalysts.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"44 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141644814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling and Analysis of Environmental Electromagnetic Interference in Multiple-Channel Neural Recording Systems for High Common-Mode Interference Rejection Performance 多通道神经记录系统中环境电磁干扰的建模与分析，实现高共模干扰抑制性能

Biosensors Pub Date : 2024-07-15 DOI: 10.3390/bios14070343

Gang Wang, Chang-Seok You, Chengcong Feng, Wenliang Yao, Zhengtuo Zhao, Ning Xue, Lei Yao

引用次数: 0

Evaluation of Transducer Elements Based on Different Material Configurations for Aptamer-Based Electrochemical Biosensors 评估基于不同材料配置的换能器元件，用于基于色聚体的电化学生物传感器

Biosensors Pub Date : 2024-07-13 DOI: 10.3390/bios14070341

Ivan Lopez Carrasco, Gianaurelio Cuniberti, Jörg Opitz, Natalia Beshchasna

{"title":"Evaluation of Transducer Elements Based on Different Material Configurations for Aptamer-Based Electrochemical Biosensors","authors":"Ivan Lopez Carrasco, Gianaurelio Cuniberti, Jörg Opitz, Natalia Beshchasna","doi":"10.3390/bios14070341","DOIUrl":"https://doi.org/10.3390/bios14070341","url":null,"abstract":"The selection of an appropriate transducer is a key element in biosensor development. Currently, a wide variety of substrates and working electrode materials utilizing different fabrication techniques are used in the field of biosensors. In the frame of this study, the following three specific material configurations with gold-finish layers were investigated regarding their efficacy to be used as electrochemical (EC) biosensors: (I) a silicone-based sensor substrate with a layer configuration of 50 nm SiO/50 nm SiN/100 nm Au/30–50 nm WTi/140 nm SiO/bulk Si); (II) polyethylene naphthalate (PEN) with a gold inkjet-printed layer; and (III) polyethylene terephthalate (PET) with a screen-printed gold layer. Electrodes were characterized using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) to evaluate their performance as electrochemical transducers in an aptamer-based biosensor for the detection of cardiac troponin I using the redox molecule hexacyanoferrade/hexacyaniferrade (K3[Fe (CN)6]/K4[Fe (CN)6]. Baseline signals were obtained from clean electrodes after a specific cleaning procedure and after functionalization with the thiolate cardiac troponin I aptamers “Tro4” and “Tro6”. With the goal of improving the PEN-based and PET-based performance, sintered PEN-based samples and PET-based samples with a carbon or silver layer under the gold were studied. The effect of a high number of immobilized aptamers will be tested in further work using the PEN-based sample. In this study, the charge-transfer resistance (Rct), anodic peak height (Ipa), cathodic peak height (Ipc) and peak separation (∆E) were determined. The PEN-based electrodes demonstrated better biosensor properties such as lower initial Rct values, a greater change in Rct after the immobilization of the Tro4 aptamer on its surface, higher Ipc and Ipa values and lower ∆E, which correlated with a higher number of immobilized aptamers compared with the other two types of samples functionalized using the same procedure.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141611473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances of Fluorescent Nanodiamond Platforms for Intracellular and On-Chip Biosensing 用于细胞内和芯片上生物传感的荧光纳米金刚石平台的研究进展

Biosensors Pub Date : 2024-07-12 DOI: 10.3390/bios14070340

Taisuke Shimada, Yasuyuki Ueda, Yoshinobu Baba, Hiroshi Yukawa

引用次数: 0

Metal Nanoparticle-Based Biosensors for the Early Diagnosis of Infectious Diseases Caused by ESKAPE Pathogens in the Fight against the Antimicrobial-Resistance Crisis 基于金属纳米粒子的生物传感器用于早期诊断 ESKAPE 病原体引起的传染病，应对抗菌药耐药性危机

Biosensors Pub Date : 2024-07-11 DOI: 10.3390/bios14070339

Juan Carlos Gutiérrez-Santana, Viridiana Rosas-Espinosa, Evelin Martinez, Esther Casiano-García, Victor Rafael Coria-Jiménez

{"title":"Metal Nanoparticle-Based Biosensors for the Early Diagnosis of Infectious Diseases Caused by ESKAPE Pathogens in the Fight against the Antimicrobial-Resistance Crisis","authors":"Juan Carlos Gutiérrez-Santana, Viridiana Rosas-Espinosa, Evelin Martinez, Esther Casiano-García, Victor Rafael Coria-Jiménez","doi":"10.3390/bios14070339","DOIUrl":"https://doi.org/10.3390/bios14070339","url":null,"abstract":"The species included in the ESKAPE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and the genus Enterobacter) have a high capacity to develop antimicrobial resistance (AMR), a health problem that is already among the leading causes of death and could kill 10 million people a year by 2050. The generation of new potentially therapeutic molecules has been insufficient to combat the AMR “crisis”, and the World Health Organization (WHO) has stated that it will seek to promote the development of rapid diagnostic strategies. The physicochemical properties of metallic nanoparticles (MNPs) have made it possible to design biosensors capable of identifying low concentrations of ESKAPE bacteria in the short term; other systems identify antimicrobial susceptibility, and some have been designed with dual activity in situ (bacterial detection and antimicrobial activity), which suggests that, in the near future, multifunctional biosensors could exist based on MNPs capable of quickly identifying bacterial pathogens in clinical niches might become commercially available. This review focuses on the use of MNP-based systems for the rapid and accurate identification of clinically important bacterial pathogens, exhibiting the necessity for exhaustive research to achieve these objectives. This review focuses on the use of metal nanoparticle-based systems for the rapid and accurate identification of clinically important bacterial pathogens.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Application of Terahertz Metamaterials Based on Metallic Strip Structures in Detection of Reverse Micelles 探索基于金属带结构的太赫兹超材料在反向胶束检测中的应用

Biosensors Pub Date : 2024-07-11 DOI: 10.3390/bios14070338

Ziqin Fu, Jin Chen, Xiangxue Chen, Yu Sun, Fengchao Wang, Jing Yang

{"title":"Exploring the Application of Terahertz Metamaterials Based on Metallic Strip Structures in Detection of Reverse Micelles","authors":"Ziqin Fu, Jin Chen, Xiangxue Chen, Yu Sun, Fengchao Wang, Jing Yang","doi":"10.3390/bios14070338","DOIUrl":"https://doi.org/10.3390/bios14070338","url":null,"abstract":"Terahertz spectroscopy has unique advantages in the study of biological molecules in aqueous solutions. However, water has a strong absorption capability in the terahertz region. Reducing the amount of liquid could decrease interference with the terahertz wave, which may, however, affect the measurement accuracy. Therefore, it is particularly important to balance the amount and water content of liquid samples. In this work, a terahertz metamaterial sensor based on metallic strips is designed, fabricated, and used to detect reverse micelles. An aqueous confinement environment in reverse micelles can improve the signal-to-noise ratio of the terahertz response. Due to “water pool” trapped in reverse micelles, the DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) solution and DOPC emulsion can successfully be identified in intensity by terahertz spectroscopy. Combined with the metamaterial sensor, an obvious frequency shift of 30 GHz can be achieved to distinguish the DOPC emulsion (5%) from the DOPC solution. This approach may provide a potential way for improving the sensitivity of detecting trace elements in a buffer solution, thus offering a valuable toolkit toward bioanalytical applications.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances of 3D Cell Co-Culture Technology Based on Microfluidic Chips 基于微流控芯片的三维细胞共培养技术的进展

Biosensors Pub Date : 2024-07-10 DOI: 10.3390/bios14070336

Can Li, Wei He, Yihua Song, Xia Zhang, Jianfei Sun, Zuojian Zhou

{"title":"Advances of 3D Cell Co-Culture Technology Based on Microfluidic Chips","authors":"Can Li, Wei He, Yihua Song, Xia Zhang, Jianfei Sun, Zuojian Zhou","doi":"10.3390/bios14070336","DOIUrl":"https://doi.org/10.3390/bios14070336","url":null,"abstract":"Cell co-culture technology aims to study the communication mechanism between cells and to better reveal the interactions and regulatory mechanisms involved in processes such as cell growth, differentiation, apoptosis, and other cellular activities. This is achieved by simulating the complex organismic environment. Such studies are of great significance for understanding the physiological and pathological processes of multicellular organisms. As an emerging cell cultivation technology, 3D cell co-culture technology, based on microfluidic chips, can efficiently, rapidly, and accurately achieve cell co-culture. This is accomplished by leveraging the unique microchannel structures and flow characteristics of microfluidic chips. The technology can simulate the native microenvironment of cell growth, providing a new technical platform for studying intercellular communication. It has been widely used in the research of oncology, immunology, neuroscience, and other fields. In this review, we summarize and provide insights into the design of cell co-culture systems on microfluidic chips, the detection methods employed in co-culture systems, and the applications of these models.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the Human Motion Recognition Method Based on Wearable 基于可穿戴设备的人体动作识别方法研究

Biosensors Pub Date : 2024-07-10 DOI: 10.3390/bios14070337

Zhao Wang, Xing Jin, Yixuan Huang, Yawen Wang

{"title":"Research on the Human Motion Recognition Method Based on Wearable","authors":"Zhao Wang, Xing Jin, Yixuan Huang, Yawen Wang","doi":"10.3390/bios14070337","DOIUrl":"https://doi.org/10.3390/bios14070337","url":null,"abstract":"The accurate analysis of human dynamic behavior is very important for overcoming the limitations of movement diversity and behavioral adaptability. In this paper, a wearable device-based human dynamic behavior recognition method is proposed. The method collects acceleration and angular velocity data through a six-axis sensor to identify information containing specific behavior characteristics in a time series. A human movement data acquisition platform, the DMP attitude solution algorithm, and the threshold algorithm are used for processing. In this experiment, ten volunteers wore wearable sensors on their bilateral forearms, upper arms, thighs, calves, and waist, and movement data for standing, walking, and jumping were collected in school corridors and laboratory environments to verify the effectiveness of this wearable human movement recognition method. The results show that the recognition accuracy for standing, walking, and jumping reaches 98.33%, 96.67%, and 94.60%, respectively, and the average recognition rate is 96.53%. Compared with similar methods, this method not only improves the recognition accuracy but also simplifies the recognition algorithm and effectively saves computing resources. This research is expected to provide a new perspective for the recognition of human dynamic behavior and promote the wider application of wearable technology in the field of daily living assistance and health management.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Invasive Brain Sensing Technologies for Modulation of Neurological Disorders 用于调节神经系统疾病的非侵入性大脑传感技术

Biosensors Pub Date : 2024-07-09 DOI: 10.3390/bios14070335

Salman Alfihed, Majed Majrashi, Muhammad Ansary, Naif Alshamrani, Shahad H. Albrahim, Abdulrahman Alsolami, Hala A. Alamari, Adnan Zaman, Dhaifallah Almutairi, Abdulaziz Kurdi, Mai M. Alzaydi, Thamer Tabbakh, Faisal Al-Otaibi

{"title":"Non-Invasive Brain Sensing Technologies for Modulation of Neurological Disorders","authors":"Salman Alfihed, Majed Majrashi, Muhammad Ansary, Naif Alshamrani, Shahad H. Albrahim, Abdulrahman Alsolami, Hala A. Alamari, Adnan Zaman, Dhaifallah Almutairi, Abdulaziz Kurdi, Mai M. Alzaydi, Thamer Tabbakh, Faisal Al-Otaibi","doi":"10.3390/bios14070335","DOIUrl":"https://doi.org/10.3390/bios14070335","url":null,"abstract":"The non-invasive brain sensing modulation technology field is experiencing rapid development, with new techniques constantly emerging. This study delves into the field of non-invasive brain neuromodulation, a safer and potentially effective approach for treating a spectrum of neurological and psychiatric disorders. Unlike traditional deep brain stimulation (DBS) surgery, non-invasive techniques employ ultrasound, electrical currents, and electromagnetic field stimulation to stimulate the brain from outside the skull, thereby eliminating surgery risks and enhancing patient comfort. This study explores the mechanisms of various modalities, including transcranial direct current stimulation (tDCS) and transcranial magnetic stimulation (TMS), highlighting their potential to address chronic pain, anxiety, Parkinson’s disease, and depression. We also probe into the concept of closed-loop neuromodulation, which personalizes stimulation based on real-time brain activity. While we acknowledge the limitations of current technologies, our study concludes by proposing future research avenues to advance this rapidly evolving field with its immense potential to revolutionize neurological and psychiatric care and lay the foundation for the continuing advancement of innovative non-invasive brain sensing technologies.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weak Value Amplification Based Optical Sensor for High Throughput Real-Time Immunoassay of SARS-CoV-2 Spike Protein 基于弱值放大的光学传感器用于 SARS-CoV-2 Spike 蛋白的高通量实时免疫测定

Biosensors Pub Date : 2024-07-08 DOI: 10.3390/bios14070332

Xiaonan Zhang, Lizhong Zhang, Han Li, Yang Xu, Lingqin Meng, Gengyu Liang, Bei Wang, Le Liu, Tian Guan, Cuixia Guo, Yonghong He

{"title":"Weak Value Amplification Based Optical Sensor for High Throughput Real-Time Immunoassay of SARS-CoV-2 Spike Protein","authors":"Xiaonan Zhang, Lizhong Zhang, Han Li, Yang Xu, Lingqin Meng, Gengyu Liang, Bei Wang, Le Liu, Tian Guan, Cuixia Guo, Yonghong He","doi":"10.3390/bios14070332","DOIUrl":"https://doi.org/10.3390/bios14070332","url":null,"abstract":"The demand for accurate and efficient immunoassays calls for the development of precise, high-throughput analysis methods. This paper introduces a novel approach utilizing a weak measurement interface sensor for immunoassays, offering a solution for high throughput analysis. Weak measurement is a precise quantum measurement method that amplifies the weak value of a system in the weak interaction through appropriate pre- and post-selection states. To facilitate the simultaneous analysis of multiple samples, we have developed a chip with six flow channels capable of conducting six immunoassays concurrently. We can perform real-time immunoassay to determine the binding characteristics of spike protein and antibody through real-time analysis of the flow channel images and calculating the relative intensity. The proposed method boasts a simple structure, eliminating the need for intricate nano processes. The spike protein concentration and relative intensity curve were fitted using the Log-Log fitting regression equation, and R2 was 0.91. Utilizing a pre-transformation approach to account for slight variations in detection sensitivity across different flow channels, the present method achieves an impressive limit of detection(LOD) of 0.85 ng/mL for the SARS-CoV-2 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, with a system standard deviation of 5.61. Furthermore, this method has been successfully verified for monitoring molecular-specific binding processes and differentiating binding capacities.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0