利用荧光酶传感器检测 pepN 活性的细菌生物膜先进成像方法

Biosensors Pub Date : 2024-09-03 DOI:10.3390/bios14090424
Javier Valverde-Pozo, Jose M. Paredes, María Eugenia García-Rubiño, María Dolores Girón, Rafael Salto, Jose M. Alvarez-Pez, Eva M. Talavera
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引用次数: 0

摘要

本研究探讨了 pepN 活性荧光传感器 DCM-Ala 在细菌生物膜中的应用,强调了生物膜在各种生物过程中的重要作用。我们采用了先进的成像技术来观察 pepN 的活性,为检查生物膜的成熟度引入了一种新方法。我们发现,过表达 pepN 能增强大肠杆菌形成生物膜的能力。研究结果表明,在整个生物膜发育过程中,pepN 的活性水平各不相同,这表明它在生物膜研究和管理方面具有潜在的应用价值。研究结果表明,该传感器的荧光发射可作为生物膜成熟度的可靠指标,所开发的成像技术可增强我们对生物膜相关过程的理解和控制。这项工作强调了创新方法在生物膜研究中的重要性,并为在生物膜管理中利用化学排放物开辟了新途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Advanced Imaging Methodology in Bacterial Biofilms with a Fluorescent Enzymatic Sensor for pepN Activity
This research explores the use of the pepN activity fluorescent sensor DCM-Ala in bacterial biofilms, emphasizing its significance due to the critical role of biofilms in various biological processes. Advanced imaging techniques were employed to visualize pepN activity, introducing a novel approach to examining biofilm maturity. We found that the overexpression of pepN increases the ability of E. coli to form biofilm. The findings demonstrate varying levels of pepN activity throughout biofilm development, suggesting potential applications in biofilm research and management. The results indicate that the fluorescent emission from this sensor could serve as a reliable indicator of biofilm maturity, and the imaging techniques developed could enhance our understanding and control of biofilm-related processes. This work highlights the importance of innovative methods in biofilm study and opens new avenues for utilizing chemical emissions in biofilm management.
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