Acta Genetica Sinica最新文献_第7页

Relationship Between Ploidy Variation of Citrus Calli and Competence for Somatic Embryogenesis 柑桔愈伤组织倍性变异与体细胞胚胎发生能力的关系

Acta Genetica Sinica Pub Date : 2006-07-01 DOI: 10.1016/S0379-4172(06)60095-4

ZHANG Jun-E, GUO Wen-Wu, DENG Xiu-Xin

引用次数: 14

Molecular Evolution of Prolactin Gene Family in Rodents 啮齿动物催乳素基因家族的分子进化

Acta Genetica Sinica Pub Date : 2006-07-01 DOI: 10.1016/S0379-4172(06)60088-7

LI Ying , ZHANG Ya-Ping

引用次数: 2

Saccharomyces cerevisiae ste20 Mutant Showing Resistance to Glucose-Induced Cell Death 酿酒酵母ste20突变体对葡萄糖诱导的细胞死亡表现出抗性

Acta Genetica Sinica Pub Date : 2006-07-01 DOI: 10.1016/S0379-4172(06)60097-8

DU Han, LIANG Ying

引用次数: 2

Mapping QTL for Biomass Yield and Its Components in Rice (Oryza sativa L.) 水稻生物量产量及其组成成分QTL定位

Acta Genetica Sinica Pub Date : 2006-07-01 DOI: 10.1016/S0379-4172(06)60090-5

LIU Gui-Fu , YANG Jian , ZHU Jun

{"title":"Mapping QTL for Biomass Yield and Its Components in Rice (Oryza sativa L.)","authors":"LIU Gui-Fu , YANG Jian , ZHU Jun","doi":"10.1016/S0379-4172(06)60090-5","DOIUrl":"10.1016/S0379-4172(06)60090-5","url":null,"abstract":"<div>Addicive effects, additive by additive epistatic effects, and their environmental interactions of QTLs are important genetic components of quantitative traits. Genetic architecture underlying rice biomass yield and its two component traits (straw yield and grain yield) were analyzed for a population of 125 DH lines from an inter-subspecific cross of IR64/Azucena. The mixed-model based composite interval mapping approach (MCIM) was used to detect QTLs, There were 12 QTLs detected with additive main effects, 27 QTLs involved in digenic interaction with aa and/or aae effects, and 18 QTLs affected by environments with ae and/or aae effects. It was revealed that epistatic effects and QE interaction effects existed on biomass yield and its component traits in rice. In addition, the genetic basis of relationships among these traits were investigated. Four QTLs and one pair of epistatic QTLs were detected to be responsible for the positive correlation between biomass yield and straw yield. Three QTLs might be responsible for the negative correlation between straw yield and grain yield. This result could partially explain the genetic basis of correlation among the three traits, and provide useful information for genetic improvement of these traits by marker-assisted selection.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60090-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26171608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Detection of Streptomycin Resistance in Mycobacterium tuberculosis Clinical Isolates Using Four Molecular Methods in China 应用四种分子方法检测中国结核分枝杆菌临床分离株链霉素耐药性

Acta Genetica Sinica Pub Date : 2006-07-01 DOI: 10.1016/S0379-4172(06)60096-6

WU Xue-Qiong , LU Yang , ZHANG Jun-Xian , LIANG Jian-Qin , ZHANG Guang-Yu , LI Hong-Min , LÜ Cui-Huan , DING Bei-Chuan

{"title":"Detection of Streptomycin Resistance in Mycobacterium tuberculosis Clinical Isolates Using Four Molecular Methods in China","authors":"WU Xue-Qiong , LU Yang , ZHANG Jun-Xian , LIANG Jian-Qin , ZHANG Guang-Yu , LI Hong-Min , LÜ Cui-Huan , DING Bei-Chuan","doi":"10.1016/S0379-4172(06)60096-6","DOIUrl":"10.1016/S0379-4172(06)60096-6","url":null,"abstract":"<div>To evaluate the relationship between mutations in rpsL or rrs genes and streptomycin (SM) resistance, we compared four molecular methods for their clinical value in the detection of SM resistance. Genotypic analysis of SM resistance in 167 M. tuberculosis clinical strains isolated from Chinese patients was performed by direct DNA sequencing, SSCP, RFLP, and reverse dot-blot hybridization (RDBH) assays. Of the 98 SM-resistant isolates, 78 (79.6%) had missense mutations in codon 43 or 88 of rpsL resulting in a Lys to Arg substitution, 6 (6.1%) had mutations of the rrs gene at positions 513 A to C or T or 516 C to T, and 14 (14.3%) had the wild-type sequence. None of the 69 SM-susceptible isolates examined had alterations in rpsL or rrs. The results of the SSCP, RFLP, and RDBH analyses for these mutations and wild-type sequences were completely consistent with DNA sequencing data. Five distinct single-nucleotide substitutions in codon 43 or 88 of rpsL gene or in position 513 or 516 of rrs gene were correctly identified in 84 of 98 (85.7%) phenotypically SM-resistant isolates by RDBH assay. Molecular analyses of the rpsL and rrs genes are useful for rapid prediction of SM resistance in most clinical strains of M. tuberculosis. Reverse dot-blot hybridization assay is a rapid, simple, and reliable method for the detection of drug resistance.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60096-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26169858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Improving QTL Mapping Resolution Based on Genotypic Sampling—a Case Using a RIL Population 基于基因型抽样提高QTL定位分辨率——以RIL群体为例

Acta Genetica Sinica Pub Date : 2006-07-01 DOI: 10.1016/S0379-4172(06)60091-7

YAN Jian-Bing, TANG Ji-Hua, MENG Yi-Jiang, MA Xi-Qing, TENG Wen-Tao, Subhash Chander, LI Lin, LI Jian-Sheng

引用次数: 11

Somatic Cell and Sperm Cell Cytogenetics in a Patient with t(14; 21) t(14)患者的体细胞和精子细胞遗传学;21)

Acta Genetica Sinica Pub Date : 2006-06-01 DOI: 10.1016/S0379-4172(06)60076-0

TANG Yan-Ping , LIU Xiu-Sheng , LIU Yuan , YANG Zhen-Rong , CHEN Yan , XIONG Cheng-Liang

引用次数: 3

Isolation and Characterization of OsGSTL1 Promoter from Rice 水稻OsGSTL1启动子的分离与鉴定

Acta Genetica Sinica Pub Date : 2006-06-01 DOI: 10.1016/S0379-4172(06)60081-4

HU Ting-Zhang , WANG Wei-Ping , CAO Kai-Ming , XIA Mian , WANG Xi-Ping

{"title":"Isolation and Characterization of OsGSTL1 Promoter from Rice","authors":"HU Ting-Zhang , WANG Wei-Ping , CAO Kai-Ming , XIA Mian , WANG Xi-Ping","doi":"10.1016/S0379-4172(06)60081-4","DOIUrl":"10.1016/S0379-4172(06)60081-4","url":null,"abstract":"<div>OsGSTL1 gene was isolated from the rice genomic library. Semi-quantitative RT-PCR analysis demonstrated that the expression of the OsGSTL1 in rice was not induced by chlorsulfuron, ethylene, abscisic acid, salicylic acid, and methyl jasmonate. In order to investigate the cis-elements of OsGSTL1 promoter, the promoter regions with different lengths were fused to the β-glucuronidase (GUS) reporter gene. All constructs were transformed into onion epidermal cells or A. thaliana plants to detect the expression patterns. In onion epidermal cells, the 160 bp fragment and longer ones were functional for directing GUS expression. In transgenic A. thaliana, the 2 155 bp upstream region of OsGSTL1 gene directed the GUS expression only in cotyledon after germination, but not in the root of young seedlings. In the later seedling, the 2 155 bp upstream region of OsGSTL1 gene directed GUS expression in roots, stems, and leaves. However, the GUS gene directed by a 1 224 bp upstream fragment is expressed in all the checked tissues. These results suggest that the spatiotemporal expression response elements of OsGSTL1 existed in the 5′-upstream region between −2 155 and −1 224 bp.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60081-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Quantitative Trait Loci for ABA Sensitivity at Seed Germination and Seedling Stages in Rice 水稻种子萌发期和苗期ABA敏感性数量性状位点的鉴定

Acta Genetica Sinica Pub Date : 2006-06-01 DOI: 10.1016/S0379-4172(06)60082-6

YOU Jun , LI Qiang , YUE Bing , XUE Wei-Ya , LUO Li-Jun , XIONG Li-Zhong

{"title":"Identification of Quantitative Trait Loci for ABA Sensitivity at Seed Germination and Seedling Stages in Rice","authors":"YOU Jun , LI Qiang , YUE Bing , XUE Wei-Ya , LUO Li-Jun , XIONG Li-Zhong","doi":"10.1016/S0379-4172(06)60082-6","DOIUrl":"10.1016/S0379-4172(06)60082-6","url":null,"abstract":"<div>Abscisic acid (ABA) is one of the important plant hormones, which plays a critical role in seed development and adaptation to abiotic stresses. The sensitivity of rice (Oryza sativa L.) to exogenous ABA at seed germination and seedling stages was investigated in the recombinant inbred line (RIL) population derived from a cross between irrigated rice Zhenshan 97 and upland rice IRAT109, using relative germination vigor (RGV), relative germination rate (RGR) and leaf rolling scores of spraying (LRS) or culturing (LRC) with ABA as sensitivity indexes. The phenotypic correlation analysis revealed that only RGV at germination stage was positively correlated to ABA sensitivity at seedling stage. QTL detection using composite interval mapping (CIM) and mixed linear model was conducted to dissect the genetic basis of ABA sensitivity, and the single-locus QTLS detected by both methods are in good agreement with each other. Five single QTLs and six pairs of epistatic QTLs were detected for ABA sensitivity at germination stage. Eight single QTLs and five pairs of epistatic QTLs were detected for ABA sensitivity at seedling stage. Two QTLs were common between LRS and LRC; and one common QTL was detected for RGV, LRS and LRC simultaneously. These results indicated that both single and epistatic loci were involved in the ABA sensitivity in rice, and the genetic basis of ABA sensitivity at seed germination and seedling stage was largely different.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60082-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Identification of Quantitative Trait Loci Controlling Drought Tolerance at Seedling Stage in Chinese Dongxiang Common Wild Rice (Oryza rufipogon Griff.) 中国东乡普通野生稻苗期抗旱性控制数量性状位点的鉴定

Acta Genetica Sinica Pub Date : 2006-06-01 DOI: 10.1016/S0379-4172(06)60084-X

ZHOU Shao-Xia, TIAN Feng, ZHU Zuo-Feng, FU Yong-Cai, WANG Xiang-Kun, SUN Chuan-Qing

{"title":"Identification of Quantitative Trait Loci Controlling Drought Tolerance at Seedling Stage in Chinese Dongxiang Common Wild Rice (Oryza rufipogon Griff.)","authors":"ZHOU Shao-Xia, TIAN Feng, ZHU Zuo-Feng, FU Yong-Cai, WANG Xiang-Kun, SUN Chuan-Qing","doi":"10.1016/S0379-4172(06)60084-X","DOIUrl":"10.1016/S0379-4172(06)60084-X","url":null,"abstract":"<div>Common wild rice (Oryza rufipogon Griff.) is the ancestor of cultivated rice (O. sativa L.), which has a greater genetic diversity and important traits that remain to be employed in cultivated rice. In this study, a set of introgression lines (BC4F5 and/or BC4F6) carrying various introgressed segments from common wild rice, collected from Dongxiang county, Jiangxi Province, China, in the background of an Indica (O. sativa L. ssp. indica) cultivar, Guichao 2, was used. A total of 12 drought-related quantitative trait loci (QTL) were identified by investigating drought tolerance of introgression lines under 30% PEG treatment at the young seedlings stage. Of these QTLs, the alleles of 4 QTLs on chromosome 2, 6 and 12 from Dongxiang common wild rice were responsible for increased drought tolerance of the introgression lines. In particular, a QTL qSDT12-2, near RM17 on chromosome 12, was consistently detected in different replications, and expressed stably under PEG stress throughout the study. It was also found that the QTLs located on different chromosomes might express at different stages.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60084-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24