Acta Genetica Sinica最新文献

Study on Tandem Repeat Sequence Variation in Sheep mtDNA D-loop Region 绵羊mtDNA d环区串联重复序列变异的研究

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60146-7

LI Xiang-Long , GONG Yuan-Fang , LIU Zheng-Zhu , ZHENG Gui-Ru , ZHOU Rong-Yan , JIN Xiao-Min , LI Lan-Hui , WANG Hai-Liang

{"title":"Study on Tandem Repeat Sequence Variation in Sheep mtDNA D-loop Region","authors":"LI Xiang-Long , GONG Yuan-Fang , LIU Zheng-Zhu , ZHENG Gui-Ru , ZHOU Rong-Yan , JIN Xiao-Min , LI Lan-Hui , WANG Hai-Liang","doi":"10.1016/S0379-4172(06)60146-7","DOIUrl":"10.1016/S0379-4172(06)60146-7","url":null,"abstract":"<div><p>The 75-nt-long tandem repeat sequence in the control region of mtDNA of 77 individuals, of which 69 were from different indigenous sheep breeds in China and 8 were from imported breeds, was sequenced and analyzed to investigate the origin and differentiation of Chinese indigenous sheep breeds and also the genetic diversities and relationships among them. A total of 28 variable sites were detected within 309 repeated sequences, among which 7 sites were singleton variable sites with two variants, 1 site was a singleton variable site with three variants, and 20 sites were parsimony informative sites with two variants. A total of 63 haplotypes were sorted from 28 polymorphic sites, among which two main and basic haplotypes, namely, Hap 1 and Hap 3 were present at a much higher proportion, at 12.94% and 30.42%, respectively. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors because of the maternal inheritance characteristics of the mtDNA. Altay sheep and Kazakstan sheep are closely related and do not differentiate significantly. Mongolian sheep and Ujumuqin sheep also share a close relationship. Tibetan sheep, Mongolian sheep, and Ujumuqin sheep have lower genetic diversity than Altay sheep and Kazakstan sheep.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1087-1095"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60146-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Differential Expression of Endogenous Ferritin Genes and Iron Homeostasis Alteration in Transgenic Tobacco Overexpressing Soybean Ferritin Gene 过表达大豆铁蛋白基因转基因烟草内源铁蛋白基因差异表达及铁稳态改变

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60150-9

JIANG Ting-Bo, DING Bao-Jian, LI Feng-Juan, YANG Chuan-Ping

{"title":"Differential Expression of Endogenous Ferritin Genes and Iron Homeostasis Alteration in Transgenic Tobacco Overexpressing Soybean Ferritin Gene","authors":"JIANG Ting-Bo, DING Bao-Jian, LI Feng-Juan, YANG Chuan-Ping","doi":"10.1016/S0379-4172(06)60150-9","DOIUrl":"10.1016/S0379-4172(06)60150-9","url":null,"abstract":"<div><p>For studying the effects of endogenous ferritin gene expressions (<em>NtFer1</em>, GenBank accession number ay083924; and <em>NtFer2</em>, GenBank accession number ay141105) on the iron homeostasis in transgenic tobacco (<em>Nicotiana tabacum</em> L.) plants expressing soybean (<em>Glycine max</em> Merr) ferritin gene (<em>SoyFer1</em>, GenBank accession number m64337), the transgenic tobacco has been produced by placing soybean ferritin cDNA cassette under the control of the CaMV 35S promoter. The exogenous gene expression was examined by both Northern- and Western-blot analyses. Comparison of endogenous ferritin gene expressions between nontransformant and transgenic tobacco plants showed that the expression of <em>NtFer1</em> was increased in the leaves of transgenic tobacco plants, whereas the <em>NtFer2</em> expression was unchanged. The iron concentration in the leaves of transgenic tobacco plants was about 1.5-folds higher than that in nontransformant. Enhanced growth of transgenic tobacco was observed at the early development stages, resulting in plant height and fresh weights significantly greater than those in the nontransformant. These results demonstrated that exogenous ferritin expression induced increased expression of at least one of the endogenous ferritin genes in transgenic tobacco plants by enhancing the ferric chelate reductase activity and iron transport ability of the root, and improved the rate of photosynthesis.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1120-1126"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60150-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Analysis of the Genetic Diversity and the Phylogenetic Evolution of Chinese Sheep Based on Cyt b Gene Sequences 基于Cyt b基因序列的中国绵羊遗传多样性及系统进化分析

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60145-5

WANG Xin , MA Yue-Hui , CHEN Hong

引用次数: 14

Inheritance Analysis of Herbicide-Resistant Transgenic Soybean Lines 转基因大豆抗除草剂品系的遗传分析

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60148-0

ZHANG Yong, YANG Bao-Yu, CHEN Shi-Yun

{"title":"Inheritance Analysis of Herbicide-Resistant Transgenic Soybean Lines","authors":"ZHANG Yong, YANG Bao-Yu, CHEN Shi-Yun","doi":"10.1016/S0379-4172(06)60148-0","DOIUrl":"10.1016/S0379-4172(06)60148-0","url":null,"abstract":"<div><p>Four transgenic soybean lines generated <em>via Agrobacterium</em>-mediated transformation were used to analyze inheritance of the transgenes. Seed chip GUS assay and herbicide leaf painting and spraying assays were applied to test the gus reporter gene and the herbicide resistant <em>bar</em> selectable marker gene, respectively. Three of the four transgenic soybean lines were stably inherited in a Mendelian fashion with co-segregation of both transgenes in a 3:1 segregation ratio in the T<sub>1</sub> progeny, indicating that both transgenes were integrated into the same locus of the soybean genome. Homozygous transgenic progeny plants were obtained in the T<sub>2</sub> generation of these lines, and the transgenes were inherited in five successive generations. However, in one transgenic line, all the T<sub>1</sub> progeny plants showed GUS negative and herbicide sensitive. Southern blotting analysis confirmed that the transgenes were passed into the T<sub>1</sub> progeny, indicating that the transgenes were both silenced. To test if the transgene silencing was due to transcriptional or post-transcriptional level, <em>Soybean mosaic virus</em> (SMV) was inoculated on leaf tissues of the T<sub>1</sub> plants to test possible reverse effects on transgene silencing. Infection with SMV did not suppress transgene silencing, suggesting that transgene silencing in this transgenic line may not be due to post-transcriptional gene silencing.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1105-1111"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60148-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Analysis of the Phylogenetic Relationships Among Several Species of Gramineae Using ACGM Markers 利用ACGM标记分析几种禾科植物的系统发育关系

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60151-0

LU Yong-Quan , YE Zi-Hong , WU Wei-Ren

引用次数: 6

Y-chromosome Genotyping and Genetic Structure of Zhuang Populations 壮族群体y染色体基因分型与遗传结构

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60143-1

CHEN Jing , LI Hui , QIN Zhen-Dong , LIU Wen-Hong , LIN Wei-Xiong , YIN Rui-Xing , JIN Li , PAN Shang-Ling

{"title":"Y-chromosome Genotyping and Genetic Structure of Zhuang Populations","authors":"CHEN Jing , LI Hui , QIN Zhen-Dong , LIU Wen-Hong , LIN Wei-Xiong , YIN Rui-Xing , JIN Li , PAN Shang-Ling","doi":"10.1016/S0379-4172(06)60143-1","DOIUrl":"10.1016/S0379-4172(06)60143-1","url":null,"abstract":"<div><p>Zhuang, the largest ethnic minority population in China, is one of the descendant groups of the ancient Bai-Yue. Linguistically, Zhuang languages are grouped into northern and southern dialects. To characterize its genetic structure, 13 East Asian-specific Y-chromosome biallelic markers and 7 Y-chromosome short tandem repeat (STR) markers were used to infer the haplogroups of Zhuang populations. Our results showed that O*, O2a, and O1 are the predominant haplogroups in Zhuang. Frequency distribution and principal component analysis showed that Zhuang was closely related to groups of Bai-Yue origin and therefore was likely to be the descendant of Bai-Yue. The results of principal component analysis and hierarchical clustering analysis contradicted the linguistically derived north-south division. Interestingly, a west-east clinal trend of haplotype frequency changes was observed, which was supported by AMOVA analysis that showed that between-population variance of east-west division was larger than that of north-south division. O* network suggested that the Hongshuihe branch was the center of Zhuang. Our study suggests that there are three major components in Zhuang. The O* and O2a constituted the original component; later, O1 was brought into Zhuang, especially eastern Zhuang; and finally, northern Han population brought O3 into the Zhuang populations.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1060-1072"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60143-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Ultrastructure and Gene Mapping of the Albino Mutant al12 in Rice (Oryza sativa L.) 水稻白化突变体al12的超微结构及基因定位

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60149-2

XIA Jiu-Cheng , WANG Yu-Ping , MA Bing-Tian , YIN Zhao-Qing , HAO Ming , KONG De-Wei , LI Shi-Gui

引用次数: 5

Comparison of Different Foreground and Background Selection Methods in Marker-Assisted Introgression 标记辅助渗入中不同前景和背景选择方法的比较

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60144-3

BAI Jun-Yan , ZHANG Qin , JIA Xiao-Ping

{"title":"Comparison of Different Foreground and Background Selection Methods in Marker-Assisted Introgression","authors":"BAI Jun-Yan , ZHANG Qin , JIA Xiao-Ping","doi":"10.1016/S0379-4172(06)60144-3","DOIUrl":"10.1016/S0379-4172(06)60144-3","url":null,"abstract":"<div><p>Three different methods for foreground selection and four different methods for background selection were compared in terms of the efficiency of marker-assisted introgression of a QTL allele from a donor line into a recipient line and also in terms of the recovery of the recipient genetic background. The results showed that for the introgression of a donor QTL allele, a direct selection on the QTL itself (when the QTL genotype can be directly identified) would ensure that the allele is successfully introgressed and rapidly fixed. However, when a direct selection on the QTL is not feasible, an indirect selection using two closely linked flanking markers can be used, which also shows similar results. For the recovery of the recipient genetic background, if the goal is to recover the whole genetic background of the recipient, genomic similarity selection or marker index selection would be the best choice: Only three generations of backcrosses were required to recover over 98% of the recipient genome. Whereas if the goal is to recover certain background traits of the recipient, MBLUP selection would give the best results, which achieved not only over 99% recovery of the recipient QTL alleles for the background traits after three generations of backcrosses, but also showed the best genetic improvement of these traits.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1073-1080"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60144-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Fluorescent Multiplex Amplification of Three X-STR Loci 三个X-STR基因座的荧光多重扩增

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60142-X

LIU Qiu-Ling , LÜ De-Jian , ZHU Jia-Zhen , LU Hui-Ling , LUO Yan-Min , FANG Qun

引用次数: 3

Powers of Multiple-Testing Procedures for Identification of Genes Significantly Differentially Expressed in Microarray Experiments 在微阵列实验中鉴定显著差异表达基因的多重测试程序的力量

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60152-2

TAN Yuan-De, YAN Heng-Mei

{"title":"Powers of Multiple-Testing Procedures for Identification of Genes Significantly Differentially Expressed in Microarray Experiments","authors":"TAN Yuan-De, YAN Heng-Mei","doi":"10.1016/S0379-4172(06)60152-2","DOIUrl":"10.1016/S0379-4172(06)60152-2","url":null,"abstract":"<div><p>Because of the high operation costs involved in microarray experiments, the determination of the number of replicates required to detect a gene significantly differentially expressed in a given multiple-testing procedure is of considerable significance. Calculation of power/replicate numbers required in multiple-testing procedures provides design guidance for microarray experiments. Based on this model and by choice of a multiple-testing procedure, expression noises based on permutation resampling can be considerably minimized. The method for mixture distribution model is suitable to various microarray data types obtained from single noise sources, or from multiple noise sources. By using the biological replicate number required in microarray experiments for a given power or by determining the power required to detect a gene significantly differentially expressed, given the sample size, or the best multiple-testing method can be chosen. As an example, a single-distribution model of t-statistic was fitted to an observed microarray dataset of 3 000 genes responsive to stroke in rat, and then used to calculate powers of four popular multiple-testing procedures to detect a gene of an expression change <em>D</em>. The results show that the B-procedure had the lowest power to detect a gene of small change among the multiple-testing procedures, whereas the BH-procedure had the highest power. However, all multiple-testing procedures had the same power to identify a gene having the largest change. Similar to a single test, the power of the BH-procedure to detect a small change does not vary as the number of genes increases, but powers of the other three multiple-testing procedures decline as the number of genes increases.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1132-1140"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60152-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1