Acta Genetica Sinica最新文献_第2页

Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits 牛FABGL基因的分子克隆及其对牛生物经济性状的影响

Acta Genetica Sinica Pub Date : 2006-12-01 DOI: 10.1016/S0379-4172(06)60147-9

MA Yun , XU Shang-Zhong , GAO Xue , REN Hong-Yan , XIN Ya-Ping , GAO Shu-Xin , ZHANG Ying-Han

{"title":"Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits","authors":"MA Yun , XU Shang-Zhong , GAO Xue , REN Hong-Yan , XIN Ya-Ping , GAO Shu-Xin , ZHANG Ying-Han","doi":"10.1016/S0379-4172(06)60147-9","DOIUrl":"10.1016/S0379-4172(06)60147-9","url":null,"abstract":"<div>The complete CDS sequence of the bovine FABGL gene was determined by homology cloning approach combined with RT-PCR and 3′- and 5′-RACE. The results of sequence analysis and bioinformatics study showed that this cDNA contained 994 nucleotides, with a 780 bp open reading frame (ORF) flanked by a 16 bp 5′-UTR (incompletely) and a 198 bp 3′-UTR. The deduced amino acid sequence (260 AA) shows 88% identity with the corresponding sequence in humans. Two single nucleotide substitutions, one located in intron 5 (I5) at position 1 065 bp (Y = C/T) (GenBank: DQ409814) and the other in intron 8 (I8) at position 1 792 bp (R = A/G), were detected using the PCR-SSCP method. Analysis of the allele frequencies of the two polymorphic sites in three different cattle breeds (Angus, Hereford, and Simmental) with different genotypes showed large differences: in locus I8, cattle with the GG genotype showed higher beef performance index (BPI) (4.283 ± 0.475 kg/cm) in comparison with cattle with the AA genotype (4.008 ± 0.465 kg/cm) (P = 0.01). Regarding the ribeye area, cattle with the GG genotype showed significantly higher ribeye area (73.380 ± 13.005 cm2) compared with cattle with the AA genotype (67.744 ± 12.777 cm2) (p = 0.05). In locus I5, some associations for the average daily gain (ADG) were found at the significance level of 0.01 between three different genotypes (CC, CT, TT): cattle with the TT genotype showed the highest ADG (0.652 ± 0.330 kg/d), whereas cattle with the CC genotype showed the lowest ADG value (0.421 ± 0.178 kg/d).</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 12","pages":"Pages 1096-1104"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60147-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26457932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of the MyoG Gene on the Partial Growth Traits in Pigs MyoG基因对猪部分生长性状的影响

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60134-0

XUE Hui-Liang , ZHOU Zhong-Xiao

{"title":"Effects of the MyoG Gene on the Partial Growth Traits in Pigs","authors":"XUE Hui-Liang , ZHOU Zhong-Xiao","doi":"10.1016/S0379-4172(06)60134-0","DOIUrl":"10.1016/S0379-4172(06)60134-0","url":null,"abstract":"<div>Single-nucleotide polymorphisms of the MyoG gene were tested using PCR-SSCP in different pig breeds including Landrace, Large White, Duroc, Shanxi Black, and Mashen pigs, and the effects of the MyoG gene on the birth weight, the weaning weight, the 6-month body weight, and the backfat thickness were also analyzed. On the basis of the published sequence of the porcine MyoG gene, ten pairs of primers were designed, and one polymorphism was found in the PCR product amplified with In2-3 primers. The results showed that: (1) the Landrace, the Large White, and the Duroc breeds differ significantly (P < 0.05) in genotype distribution from the Shanxi Black and the Mashen breeds; (2) On the basis of the fixed effect model, significant differences were found in the birth weight and the backfat thickness among the different MyoG genotypes, whereas no significant differences existed in the weaning weight and the 6-month body weight; (3) Using least square analysis, it was seen that individuals of the BB genotype had significantly less (P < 0.01) birth weight than those of the AA and AB genotypes, with the order being AA>AB>BB; the pigs of the AA genotype had significantly lower (P < 0.01) backfat thickness than those of the AB and BB genotypes, with the order being AA<AB<BB. These results suggest that the genotype has significant effects on the individual birth weight and the backfat thickness, and that the selection of the A allele is favored with regard to the birth weight and the backfat thickness.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 992-997"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60134-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26371116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Application of TILLING in Plant Improvement 耕作技术在植物改良中的应用

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60130-3

WANG De-Kai , SUN Zong-Xiu , TAO Yue-Zhi

引用次数: 14

Analysis of Amylose Accumulation During Seed Development in Maize 玉米种子发育过程中直链淀粉积累的分析

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60137-6

GUO Shang-Jing , LI Jia-Rui , QIAO Wei-Hua , ZHANG Xian-Sheng

引用次数: 13

Carrot Antifreeze Protein Does Not Exhibit the Polygalacturonase-inhibiting Activity of PGIP Family 胡萝卜抗冻蛋白不具有PGIP家族的聚半乳糖醛酸酶抑制活性

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60139-X

ZHANG Dang-Quan , WANG Hong-Bin , LIU Bin , FENG Dong-Ru , HE Yan-Ming , WANG Jin-Fa

{"title":"Carrot Antifreeze Protein Does Not Exhibit the Polygalacturonase-inhibiting Activity of PGIP Family","authors":"ZHANG Dang-Quan , WANG Hong-Bin , LIU Bin , FENG Dong-Ru , HE Yan-Ming , WANG Jin-Fa","doi":"10.1016/S0379-4172(06)60139-X","DOIUrl":"10.1016/S0379-4172(06)60139-X","url":null,"abstract":"<div>The carrot (Daucus carota) antifreeze protein (DcAFP) has a strong antifreeze activity and identified as belonging to the plant polygalacturonase-inhibiting protein (PGIP) family based on its sequence similarities, including the presence of a leucine-rich repeat (LRR) motif. In this study, yeast two-hybrid technology was used to analyze whether the carrot AFP could act as a PGIP. The complete DcAFP and polygalacturonase (PGase; obtained from fungus Alternaria alternata by RT-PCR) coding sequences were cloned into the bait and capture vectors, respectively, and yeast two-hybrid assays were performed. The results revealed that there was no evidence of an interaction between DcAFP and PGase, which suggests that DcAFP probably lacks PGIP activity. An analysis of the electrostatic potential of DcAFP and other PGIPs revealed that a large number of nonconservative residues within the β-helix of the DcAFP LRR motif had been substituted to basic amino acids, thus changing the surface from negative to positive. This will electrostatically prevent DcAFP from binding with the positively charged surface of PGase. This is the first report that showed the correlation between nonconservative amino acids within the LRR motif of the DcAFP and its loss of polygalacturonase inhibiting activity.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 1027-1036"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60139-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Cloning and Characterization of Bombyx mori PP-BP a Gene Induced by Viral Infection 家蚕PP-BP a基因的克隆与鉴定

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60132-7

HU Zhi-Gang, CHEN Ke-Ping, YAO Qin, GAO Gui-Tian, XU Jia-Ping, CHEN Hui-Qing

{"title":"Cloning and Characterization of Bombyx mori PP-BP a Gene Induced by Viral Infection","authors":"HU Zhi-Gang, CHEN Ke-Ping, YAO Qin, GAO Gui-Tian, XU Jia-Ping, CHEN Hui-Qing","doi":"10.1016/S0379-4172(06)60132-7","DOIUrl":"10.1016/S0379-4172(06)60132-7","url":null,"abstract":"<div>The ENF peptide family, so termed after the consensus sequence in their amino termini (Glu-Asn-Phe-), is assumed to play multiple important roles in defense reactions, growth regulation, and homeostasis of Lepidopteran insects. The paralytic peptide of Bombyx mori (BmPP) is one such peptide that is involved in the paralytic and plasmatocyte-spreading activities in the hemocyte immune reaction. The growth-blocking peptide of Pseudaletia separata (PsGBP), which is also a member of the ENF peptide family, has similar functions that can reportedly be attenuated by the growth-blocking peptide-binding protein (GBP-BP). Using the fluorescent differential display (FDD) technique, the differential expression pattern of genes in highly susceptible silkworm strain 306 were analyzed, following infection with B. mori nuclear polyhedrosis virus (BmNPV), and a differential band (G12782) was obtained from the hemolymph RNA pools. Using 5′-RACE with a specially designed primer based on the FDD study, a 1 401 bp cDNA clone was obtained containing a 1 311 bp open reading frame (ORF, GenBank accession number DQ306881). The deduced protein was highly homologous in primary structure to GBP-BP and was termed B. mori paralytic peptide-binding protein (PP-BP). The B. mori PP-BP gene is organized into two exons and only one intron, using bioinformatics searches.Using RT-PCR analysis, it was found that the B. mori PP-BP gene was expressed almost exclusively in the hemolymph. Real-time quantitative PCR analysis indicated that the B. mori PP-BP mRNA level in B. mori strain 306 exposed to BmNPV was much higher than that in B. mori strain without the virus infection. This result implies that the B. mori PP-BP is related to the cellular immune response after BmNPV invades the hemolymph.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 975-983"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60132-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26371114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Analysis of Expressed Sequence Tags from Skeletal Muscle-specific cDNA Library of Chinese Native Xiang Pig 中国乡土湘猪骨骼肌特异性cDNA文库表达序列标签分析

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60133-9

WANG Xiu-Li , WU Ke-Liang , LI Ning , LI Chang-Lv , QIU Xue-Mei , WANG Ai-Hua , WU Chang-Xin

{"title":"Analysis of Expressed Sequence Tags from Skeletal Muscle-specific cDNA Library of Chinese Native Xiang Pig","authors":"WANG Xiu-Li , WU Ke-Liang , LI Ning , LI Chang-Lv , QIU Xue-Mei , WANG Ai-Hua , WU Chang-Xin","doi":"10.1016/S0379-4172(06)60133-9","DOIUrl":"10.1016/S0379-4172(06)60133-9","url":null,"abstract":"<div>A Longissimus Dorsi muscle cDNA library of Xiang Pig was constructed, and 131 randomly isolated clones were sequenced in this study. The results of bioinformatics analysis showed that 131 ESTs represented 109 unique clones sequences, of which 99 showed homology to previously identified genes in humans or other mammals, 3 matched other uncharacterized expressed sequence tags (ESTs), and 7 showed no significant matches to sequences already present in DNA databases. No protein matches were found for 10 ESTs. Functional analysis of the ESTs showed that a considerable proportion of them encoded proteins involved in gene/protein expression (45.46%). Other classes included genes involved in metabolism (10.10%), cell structure/motility (10.10%), cell/organism defense (5.05%), cell signaling/communication (2.02%), and cell division (0.0%). Unclassified genes constituted the remaining 27.27%. This study reported the results of the first gene expression profile analysis of Chinese native Xiang Pig skeletal muscle cells, thereby greatly facilitating the functional study of candidate genes involved in muscle growth as well as in the improvement of meat quality in domestic pigs.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 984-991"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60133-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26371115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Genetic Relationships of Ornamental Cultivars of Ginkgo biloba Analyzed by AFLP Techniques 银杏观赏品种亲缘关系的AFLP分析

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60138-8

WANG Li , XING Shi-Yan , YANG Ke-Qiang , WANG Zheng-Hua , GUO Yan-Yan , SHU Huai-Rui

{"title":"Genetic Relationships of Ornamental Cultivars of Ginkgo biloba Analyzed by AFLP Techniques","authors":"WANG Li , XING Shi-Yan , YANG Ke-Qiang , WANG Zheng-Hua , GUO Yan-Yan , SHU Huai-Rui","doi":"10.1016/S0379-4172(06)60138-8","DOIUrl":"10.1016/S0379-4172(06)60138-8","url":null,"abstract":"<div>Eight primer combinations that produced clear and a large number of polymorphic bands were screened from 64 EcoR I/Mse I primer combinations (Mse I fluorescent labeled). The genetic relationships of 21 ornamental cultivars of Ginkgo biloba L. from the United States of America, Holland, Japan, France, and China were analyzed. These primer combinations produced a total of 1 119 bands, 229 specific loci (including 54 absent bands, and 175 monomorphic bands). Among them, 983 polymorphic bands (PPB), accounting for 88%, were detected. The percentage of identification per primer combination was as high as 100%. The average PPB of 14 foreign cultivars was 35.86% and the average PPB of seven domestic cultivars was 31.51%. Genetic similarity coefficient (SC) among all cultivars varied from 0.4899 to 0.8499, and all cultivars were divided into the four clusters when SC was set at 0.7300. The cultivars from the same origin did not fall into the same group. The cultivars from France and China were classified into three groups. According to the comprehensive analyses based on specific loci, similarity coefficient, and clustering results, eight cultivars ‘Fastigiata’, ‘Tit’, ‘Tubifolia’, ‘Daeryinxing’, ‘Variegata’, ‘Horizontalis, ‘Pendula’, and ‘Yiyuanyeziyinxing’ were considered to be important germplasms of ornamental cultivars of Ginkgo biloba.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 1020-1026"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60138-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4 血杆菌C4几丁质酶基因的克隆与表达

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60140-6

TAO Yong , JIN Hong , LONG Zhang-Fu , ZHANG Li , DING Xiu-Qiong , TAO Ke , LIU Shi-Gui

{"title":"Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4","authors":"TAO Yong , JIN Hong , LONG Zhang-Fu , ZHANG Li , DING Xiu-Qiong , TAO Ke , LIU Shi-Gui","doi":"10.1016/S0379-4172(06)60140-6","DOIUrl":"10.1016/S0379-4172(06)60140-6","url":null,"abstract":"<div>The chitinase Chi58 is an extracellular chitinase produced by Sanguibacter sp.strain C4. The gene-specific PCR primers were used to detect the presence of the chiA gene in strain C4. A chiA fragment (chiA-F) was amplified from the C4 genomic DNA and was used to blast-search the related sequences from the GenBank dadabase. By alignment and selection of the highly conserved regions of the homologous sequences, two pairs of primers were designed to amplify the open reading frame (ORF) of the chitinase from strain C4 by nested PCR. The results revealed that the Chi58 ORF consisted of 1 692 nucleotides encoding a protein of 563 amino acid residues. The molecular weight of the mature protein was predicted to be 58.544 kDa. The Chi58 ORF was a modular enzyme composed of a signal peptide sequence, a polycystic kidney disease I domain, and a glycosyl hydrolase family 18 domain. The chitinase of C4 exhibited a high level of similarity to the chitinase A of Serratia (88.9%–99.6%) at the amino acid sequence level. The Chi58 gene was cloned into the expression vector pET32a to construct the recombinant plasmid pChi58 and was expressed in E. coli BL-21 (DE3) cells with IPTG induction. The molecular weight of the Trx-Chi58 fusion protein was estimated to be 81.1 kDa by SDS-PAGE.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 1037-1046"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60140-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Sequence Analysis of a Novel Insertion Site of Transposon IS10 转座子IS10新插入位点的序列分析

Acta Genetica Sinica Pub Date : 2006-11-01 DOI: 10.1016/S0379-4172(06)60141-8

XIANG Tai-He, WANG Li-Lin, WANG Hui-Zhong

引用次数: 3