Acta Genetica Sinica最新文献

{"title":"Genetic and QTL Analysis for Low-Temperature Vigor of Germination in Rice","authors":"HAN Long-Zhi ,&nbsp;ZHANG Yuan-Yuan ,&nbsp;QIAO Yong-Li ,&nbsp;CAO Gui-Lan ,&nbsp;ZHANG San-Yuan ,&nbsp;KIM Jong-Hwan ,&nbsp;KOH Hee-Jong","doi":"10.1016/S0379-4172(06)60135-2","DOIUrl":"10.1016/S0379-4172(06)60135-2","url":null,"abstract":"<div><p>The quantitative trait loci (QTLs) for low-temperature vigor of germination (LVG) with a germination period of 7 d, 11 d, 14 d, and 17 d at 14 °C was identified using F_2:3 population, which included 200 individuals and lines derived from a cross of <em>indica</em> and <em>japonica</em> “Milyang 23/Jileng 1” with microsatellite markers. The correlation coefficient between LVG and other cold tolerance traits was analyzed. LVG and the cold response index for vigor of germination (CIVG) detected when the germination period was 7 d showed a continuous distribution, which was partial to lower LVG and lower CIVG in F₃ lines. LVG and CIVG detected when the germination periods were 11 d, 14 d, and 17 d showed a continuous distribution near normal, which were quantitative traits controlled by multiple genes. LVG detected when the germination period was 14 d was more correlated with other cold tolerance traits than LVG detected when the germination periods were 7 d, 11 d, and 17 d, which was significantly associated with cold tolerance during the bud bursting period, the seedling stage, the booting stage, and the growing ability under cold conditions. <em>qLVG2</em> located in RM29-RM262 on chromosome 2, <em>qLVG7-2</em> and <em>qCIVG7-2</em> located in RM336-RM118 on chromosome 7 were detected when the germination periods were 11 d, 14 d, and 17 d. <em>qCIVG2</em> located in RM29-RM262 on chromosome 2 was detected when the germination periods were 11 d and 14 d. The variation is due to the observed phenotypic variation by the above QTLs, which was increased following the germination. The variation of <em>qLVG2</em> related to LVG was increased from 6.9% to 14.2%. The variation of <em>qLVG7-2</em> associated with LVG was increased from 9.9% to 11.2%. The variation of <em>qCIVG2</em> correlated with CIVG was increased from 6.3% to 9.0%. The variation of <em>qCIVG7-2</em> associated with CIVG was increased from 8.3% to 12.9%. These QTL alleles were obtained from the tolerant parent Jileng 1, and the gene action was most likely to be partially dominant.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 998-1006"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60135-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26371117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Complete Mitochondrial DNA Control Region of Four Species of Strigiformes","authors":"XIAO Bing ,&nbsp;MA Fei ,&nbsp;SUN Yi ,&nbsp;LI Qing-Wei","doi":"10.1016/S0379-4172(06)60131-5","DOIUrl":"10.1016/S0379-4172(06)60131-5","url":null,"abstract":"<div><p>The sequence of the whole mitochondrial (mt) DNA control region (CR) of four species of Strigiformes was obtained. Length of the CR was 3 290 bp, 2 848 bp, 2 444 bp, and 1 771 bp for <em>Asio flammeus, Asio otus, Athene noctua,</em> and <em>Strix aluco</em>, respectively. Interestingly, the length of the control region was maximum in <em>Asio flammeus</em> among all the avian mtDNA control regions sequenced thus far. In addition, the base composition and organization of mtDNA CR of <em>Asio flammeus</em> were identical to those reported for other birds. On the basis of the differential frequencies of base substitutions, the CR may be divided two variable domains, I and III, and a central conserved domain, II. The 3′ end of the CR contained many tandem repeats of varying lengths and repeat numbers. In <em>Asio flammeus</em>, the repeated sequences consisted of a 126 bp sequence that was repeated seven times and a 78 bp sequence that was repeated 14 times. In <em>Asio otus</em>, there were also two repeated sequences, namely a 127 bp sequence that was repeated eight times and a 78 bp sequence that was repeated six times. The control region of <em>Athene noctua</em> contained three sets of repeats: a 89 bp sequence that was repeated three times, a 77 bp sequence that was repeated four times, and a 71 bp sequence that was repeated six times. <em>Strix aluco</em>, however, had only one repeated sequence, a 78 bp sequence that was repeated five times. The results of this study seem to indicate that these tandem repeats may have resulted from slipped-strand mispairing during mtDNA replication. Moreover, there are many conserved motifs within the repeated units. These sequences could form stable stem-loop secondary structures, which suggests that these repeated sequences play an important role in regulating transcription and replication of the mitochondrial genome.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 965-974"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60131-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26371113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Genotype × Environment Interaction Effects for Starch Pasting Viscosity Characteristics in Indica Rice","authors":"BAO Jin-Song,&nbsp;SHEN Sheng-Quan,&nbsp;XIA Ying-Wu","doi":"10.1016/S0379-4172(06)60136-4","DOIUrl":"10.1016/S0379-4172(06)60136-4","url":null,"abstract":"<div><p>Milled rice (<em>Oryza sativa</em> L.) is composed of approximately 90% starch. The properties of starch have considerable effects on cooked rice palatability and consumer acceptability. Starch pasting viscosity parameters serve as important indices in the estimation of eating, cooking, and processing qualities of rice. In the present study, four cytoplasmic male-sterile (CMS) lines and eight restorer (R) lines have been used in an incomplete diallel cross to analyze seed effects, cytoplasmic effects, maternal gene effects, and their genotype × environment (GE) effects on the following starch pasting viscosity parameters: breakdown (BD), consistency (CS), and setback (SB). The results demonstrated that the total main genetic variances (<em>V_G</em>) accounted for over 64% of the total genetic variance (<em>V_G</em> + <em>V_GE</em>) for the three traits, indicating that these traits were mainly controlled by the main genetic effects in addition to the GE interaction effects. The estimated total narrow-sense heritability were 67.8%, 79.5%, and 79.5% for BD, CS, and SB, respectively. The general heritability (<em>h²_G</em>) accounted for over 75% of the total heritability (<em>h²_G</em> + <em>h²_GE</em>), indicating that early selection would be effective for those traits and the selection efficiencies were relatively stable in different environments.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 11","pages":"Pages 1007-1013"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60136-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26371118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microarray Analysis of Gene-Expression Profile in Hepatocellular Carcinoma Cell, BEL-7402, with Stable Suppression of hLRH-1 via a DNA Vector-based RNA Interference","authors":"WANG Shui-Liang ,&nbsp;LAN Feng-Hua ,&nbsp;ZHUANG Yue-Peng ,&nbsp;LI Hui-Zhong ,&nbsp;HUANG Liang-Hu ,&nbsp;ZHENG De-Zhu ,&nbsp;ZENG Jian ,&nbsp;DONG Li-Hong ,&nbsp;ZHU Zhong-Yong ,&nbsp;FU Ji-Liang","doi":"10.1016/S0379-4172(06)60122-4","DOIUrl":"10.1016/S0379-4172(06)60122-4","url":null,"abstract":"<div><p>To establish a cell line with a permanent suppression of hLRH-1 in this study, a stable RNAi vector (pSineo<em>hLRH-1</em>) targeting <em>hLRH-1</em> was constructed and introduced into hepatocellular carcinoma cell, BEL-7402. By semiquantitative RT-PCR analysis, the expression of <em>hLRH-1</em> in BEL-7402 cells carrying pSineo<em>hLRH-1</em> was shown to be significantly suppressed by up to ∼60%. In addition, microarray analysis was carried out to assess the extent of altered gene expression in BEL-7402 cells with stable knockdown of hLRH-1. Direct comparison of gene-expression profiles of more than 18 000 genes showed that 405 of the expressed genes in hLRH-1-knockdown cells differed dramatically in expression levels from those in controls, which suggested the even extensive biological functions of hLRH-1. Interestingly, among those differentially expressed genes, some are cancer-associated such as Gadd45β and PTEN, and their expressions were further validated. Although the identification of the exact relationship between these genes and hLRH-1 awaits intensive investigation, the findings of this study provide new insights into the mechanism by which hLRH-1 is involved in tumorigenesis.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 881-891"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60122-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26313888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Differentially Expressed Genes in the High and Low Metastatic Human Ovarian Cancer Cell Lines and Analyses of Their Chromosomal Localizations and Functions","authors":"XU Shen-Hua,&nbsp;MU Han-Zhou,&nbsp;GU Lin-Hui,&nbsp;ZHU Chi-Hong,&nbsp;LIU Xiang-Lin","doi":"10.1016/S0379-4172(06)60123-6","DOIUrl":"10.1016/S0379-4172(06)60123-6","url":null,"abstract":"<div><p>Oligonucleotide microarrays were used to study the differences of gene expressions in high (H) and low (L) metastatic ovarian cancer cell lines and in normal ovarian tissues (C). Bioinformatics was used to identify novel genes and their functions as well as chromosomal localizations. A total of 409 genes were differentially expressed between the high and low metastatic ovarian cancer cell lines. Of them, 271 genes were up regulated (Signal Log Ratio[SLR] ≥1), and 138 genes were down regulated (SLR≤-1). Except one gene whose location was unknown, all these genes were localized randomly on all the chromosomes, with a majority of them localized to Chromosomes 1, 6, 2, 17, 3, 5 and 11. Chromosome 1 contained, 43 of them (10.7%), the most for a single chromosome. A total of 264 genes (64.7%) were localized on the short arm of the chromosome (q). Functional classification showed that the 104 (25.4%) genes coding for enzymes and enzyme regulators made up the largest functional group, followed by signal transduction activity genes (43, 10.5%), nucleic acid binding activity genes (42, 10.3%), and proteins binding activity genes (34, 8.3%). These four groups accounted for 54.5% of all the differentially expressed genes. In addition, the functions of 76 genes (18.6%) were unknown. Tumor metastasis is the result of a number of genes acting in concert. The four functional groups of genes classified among these genes and their abnormalities would be the focus of further studies on ovarian cancer metastasis.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 892-900"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60123-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26313889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shrinkage Estimation Method for Mapping Multiple Quantitative Trait Loci","authors":"ZHANG Yuan-Ming","doi":"10.1016/S0379-4172(06)60120-0","DOIUrl":"10.1016/S0379-4172(06)60120-0","url":null,"abstract":"<div><p>In this article, shrinkage estimation method for multiple-marker analysis and for mapping multiple quantitative trait loci (QTL) was reviewed. For multiple-marker analysis, Xu (Genetics, 2003, 163:789-801) developed a Bayesian shrinkage estimation (BSE) method. The key to the success of this method is to allow each marker effect have its own variance parameter, which in turn has its own prior distribution so that the variance can be estimated from the data. Under this hierarchical model, a large number of markers can be handled although most of them may have negligible effects. Under epistatic genetic model, however, the running time is very long. To overcome this problem, a novel method of incorporating the idea described above into maximum likelihood, known as penalized likelihood method, was proposed. A simulated study showed that this method can handle a model with multiple effects, which are ten times larger than the sample size. For multiple QTL analysis, two modified versions for the BSE method were introduced: one is the fixed-interval method and another is the variable-interval method. The former deals with markers with intermediate density, and the latter can handle markers with extremely high density as well as model with epistatic effects. For the detection of epistatic effects, penalized likelihood method and the variable-interval approach of the BSE method are available.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 861-869"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60120-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26313886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Polymorphism of Microsatellite DNA in Two Populations of Northern Sheatfish (Silurus soldatovi)","authors":"QUAN Ying-Chun ,&nbsp;SUN Xiao-Wen ,&nbsp;LIANG Li-Qun","doi":"10.1016/S0379-4172(06)60125-X","DOIUrl":"10.1016/S0379-4172(06)60125-X","url":null,"abstract":"<div><p>In this article, population variations and genetic structures of two populations of northern sheatfish (<em>Silurus soldatovi</em>) were analyzed using 24 microsatellite loci enriched from southern catfish (<em>S. meriaionalis</em> Chen) by magnetic beads. Gene frequency (<em>P</em>), observed heterozygosity (<em>Ho</em>), expected heterozygosity (<em>He</em>), polymorphism information contents (<em>PIC</em>), and number of effective alleles (<em>Ne</em>) were determined. One population was wild, ripe individuals collected from Heilongjiang River (<em>HNS</em>); the other was cultured fry collected from Songhuajiang River (<em>SNS</em>). The Hardy-Weinberg equilibrium (HWE) was tested by the genetic departure index (<em>d</em>). The coefficient of gene differentiation <em>G_ST</em> and Φ_<em>ST</em> by AMOVA (Analysis of Molecular Variety) was imputed using Arlequin software in this study. In addition, a phylogenetic tree was constructed by UPGMA method based on the pairwise Nei's standard distances using PHYLIP. A total of 1 357 fragments with sizes ranging between 102 bp and 385 bp were acquired by PCR amplifications. The average number of alleles of the two populations was 8.875. Results indicated that these microsatellite loci were highly polymorphic and could be used as genetic markers. The mean values of the parameters <em>P, Ho, He, PIC,</em> and <em>Ne</em> were 0.165, 0.435, 0.758, 0.742, and 5.019 for <em>HNS</em> and 0.147, 0.299, 0.847, 0.764, and 5.944 for <em>SNS</em>, respectively. Although there were differences, there were no significant differentiations except for the locus <em>HLJcf37</em>. These populations to a certain extent deviated from HWE, such as excessive and deficient heterozygote numbers. The value of <em>G_ST</em> was 0.078 and above 98% of the variation were differences among individuals within the population, so the variation between populations was insignificant. Cluster analysis also showed that the relationships among individuals were very close. In conclusion, the microsatellite markers that were developed through this study are useful for genetic analysis and the genetic culture that was proposed in this study has no significant impact on <em>S. soldatovi</em>.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 908-916"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60125-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26313891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Gene Differential Expression of Leaves in Full Opening Flower Stages of Hybrids & Their Parents and Heterosis in Pest-resistant Cotton","authors":"XING Chao-Zhu ,&nbsp;ZHAO Yun-Lei ,&nbsp;YU Shu-Xun ,&nbsp;ZHANG Xian-Long ,&nbsp;GUO Li-Ping ,&nbsp;WANG Hai-Lin","doi":"10.1016/S0379-4172(06)60129-7","DOIUrl":"10.1016/S0379-4172(06)60129-7","url":null,"abstract":"<div><p>cDNAs of leaves of 24 pest-resistant cotton (authorization) crosses and their parents in full opening flower stages were amplified and different bands were displayed by DDRT-PCR, with 45 primer combinations. The mean percentage of bands duplicated was 70.1%, which implied that there was a high proportion of pseudopositive fragments in the amplified cDNA. These pseudopositive bands can be reduced using duplicate PCR. Correlation analysis between differential gene expression and hybrid performance and heterosis showed that M4 (bands detected in one parent and F₁) was positively correlated with all yield traits, and significantly correlated with boll number; M2 (bands observed in one parent but not in F₁ and another parent) was negatively correlated with heterosis of boll number; and M3 (bands observed only in F₁) was positively correlated with heterosis of lint yield. These results showed that genes that had dominant and specific expression in top leaves of full opening flower stages were beneficial to yield formation and heterosis occurrence.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 948-956"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60129-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26314383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Human Calcium-Sensing Receptor and Interleukin-6 Genes are Associated with Bone Mineral Density in Chinese","authors":"WANG Yan-Bo ,&nbsp;GUO Jing-Jing ,&nbsp;LIU Yong-Jun ,&nbsp;DENG Fei-Yan ,&nbsp;JIANG De-Ke ,&nbsp;DENG Hong-Wen","doi":"10.1016/S0379-4172(06)60121-2","DOIUrl":"10.1016/S0379-4172(06)60121-2","url":null,"abstract":"<div><p>Calcium sensing receptor (CASR) is a central factor involved in calcium metabolism. Interleukin-6 (IL-6) is a pleiotropic cytokine that plays an important role in osteoclast differentiation. Thus, both <em>CASR</em> and <em>IL-6</em> are important in bone and mineral metabolism and are prominent candidate genes for osteoporosis. The study aimed to test association and/or linkage between the <em>CASR</em> and <em>IL-6</em> genes with bone mineral density (BMD) variation in a Chinese population. A cytosine-adenine (<em>CA</em>)_<em>n</em> repeat polymorphism in the <em>CASR</em> gene and the <em>IL-6</em> gene was genotyped, respectively, in 1 263 subjects from 402 Chinese nuclear families. Employing tests implemented in the program QTDT (quantitative transmission disequilibrium tests), a significant total association of the <em>CASR</em> (CA)₁₂ allele (<em>P</em> = 0.006) and (CA)₁₈ allele (<em>P</em> = 0.02) with BMD at the femoral neck was found. For the <em>IL-6</em> gene, significant within-family associations were found between the (<em>CA</em>)₁₄ allele and BMD at the total hip (<em>P</em> = 0.021), the femoral neck (<em>P</em> = 0.041), and the intertrochanteric region (<em>P</em> = 0.029). A significant linkage was also observed between <em>IL-6 CA</em> repeat polymorphism and BMD at the spine (<em>P</em> = 0.001). The results suggest that the <em>CASR</em> gene and the <em>IL-6</em> gene may have effects on BMD variation in Chinese.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 870-880"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60121-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26313887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity Analysis of Tibetan Wild Barley Using SSR Markers","authors":"FENG Zong-Yun ,&nbsp;LIU Xian-Jun ,&nbsp;ZHANG Yi-Zheng ,&nbsp;LING Hong-Qing","doi":"10.1016/S0379-4172(06)60126-1","DOIUrl":"10.1016/S0379-4172(06)60126-1","url":null,"abstract":"<div><p>One hundred and six accessions of wild barley collected from Tibet, China, including 50 entries of the two-rowed wild barley <em>Hordeum vulgare</em> ssp. <em>spontaneum</em> (HS), 29 entries of the six-rowed wild barley <em>Hordeum vulgare</em> ssp. <em>agriocrithon</em> (HA), and 27 entries of the six-rowed wild barley <em>Hordeum vulgare</em> ssp. <em>agriocrithon</em> var. <em>lagunculiforme</em> (HL), were analyzed using 30 SSR markers selected from the seven barley linkage groups for studying genetic diversity and evolutionary relationship of the three subspecies of Tibetan wild barley to cultivated barley in China. Over the 30 genetic loci that were studied, 229 alleles were identified among the 106 accessions, of which 70 were common alleles. <em>H. vulgare</em> ssp. <em>spontaneum</em> possesses about thrice more private alleles (2.83 alleles/locus) than HS (0.93 alleles/locus), whereas almost no private alleles were detected in HL. The genetic diversity among-subspecies is much higher than that within-subspecies. Generally, the genetic diversity among the three subspecies is of the order HS &gt; HL &gt; HA. Phylogenetic analysis of the 106 accessions showed that all the accessions of HS and HA was clustered in their own groups, whereas the 27 accessions of HL were separated into two groups (14 entries with group HS and the rest with group HA). This indicated that HL was an intermediate form between HS and HA. Based on this study and previous works, we suggested that Chinese cultivated barley might evolve from HS via HL to HA.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 917-928"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60126-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26314380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}