Acta Genetica Sinica最新文献_第10页

Effects of degU32(Hy), degQa and degR Pleiotropic Regulatory Genes on the Growth and Protease Fermentation of Bacillus Subtilis Ki-2-132 degU32(Hy)、degQa和degR多效调控基因对枯草芽孢杆菌Ki-2-132生长和蛋白酶发酵的影响

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60063-2

PAN Xue-Feng

引用次数: 2

Screening for and Genetic Analysis on T-DNA-inserted Mutant Pool in Rice 水稻t - dna插入突变体库的筛选与遗传分析

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60057-7

LI Ai-Hong , ZHANG Ya-Fang , WU Chang-Yin , TANG Wen , WU Ru , DAI Zheng-Yuan , LIU Guang-Qing , ZHANG Hong-Xi , PAN Xue-Biao

{"title":"Screening for and Genetic Analysis on T-DNA-inserted Mutant Pool in Rice","authors":"LI Ai-Hong , ZHANG Ya-Fang , WU Chang-Yin , TANG Wen , WU Ru , DAI Zheng-Yuan , LIU Guang-Qing , ZHANG Hong-Xi , PAN Xue-Biao","doi":"10.1016/S0379-4172(06)60057-7","DOIUrl":"10.1016/S0379-4172(06)60057-7","url":null,"abstract":"<div><p>T-DNA tagging technique has provided a powerful strategy for identifying new functional genes in plants, and the key for success is the discovery of T-DNA-inserted mutants with changed phenotype. In this study, we screened 4 416 rice T<sub>1</sub> tagged lines generated by enhancer trap system integrated with GLL4/VP16-UAS elements from two transformed parents, ZH11 and ZH15. We found many lines showed obvious morphological mutations, including two types—fake-homozygous mutation and separating mutation. The mutation phenotype was related to 14 kinds of trait such as plant height, heading date, leaf shape, leaf color, tiller number, panicle shape, spikelet number, grain shape, disease-like mutation, male sterility, awn, and so on. Among them, plant height, heading date, leaf color and male sterility had a comparatively high mutation frequency (over 1%). The mutation frequency of plant height and leaf color had no significant change between different years or transformed parents, but the frequency of heading date and male sterility varied greatly, suggesting that environment had a great effect on the expression of latter two traits. By conducting continuously co-segregating analyses in T<sub>1</sub> and T<sub>2</sub> generation, we identified 3 T-DNA-inserted mutants with malformed panicle or spikelets, which would provide a base for cloning correlative functional genes. At the same time, we selected randomly 42 lines with mutation phenotype and obtained 40 flanking sequences from 39 tagged lines by plasmid rescue or TAIL-PCR, of which, 26 were vector backbone sequence, 14 had good identity to rice genome sequence. The BlastN result showed the T-DNA preferentially integrated into protein-coding region in plants.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60057-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Evidence of Different Ploidy Eggs Produced by Diploid F2 Hybrids of Carassius auratus (♀) × Cyprinus carpio (♂) 鲫鱼(♀)与鲫鱼(♂)二倍体F2杂交产生不同倍性卵的证据

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60055-3

LIU Shao-Jun, SUN Yuan-Dong, LUO Kai-Kun, LIU Yun

{"title":"Evidence of Different Ploidy Eggs Produced by Diploid F2 Hybrids of Carassius auratus (♀) × Cyprinus carpio (♂)","authors":"LIU Shao-Jun, SUN Yuan-Dong, LUO Kai-Kun, LIU Yun","doi":"10.1016/S0379-4172(06)60055-3","DOIUrl":"https://doi.org/10.1016/S0379-4172(06)60055-3","url":null,"abstract":"<div><p>Based on the presence of three types of eggs with different diameters 0.13, 0.17 and 0.2 cm, we made two crosses: F<sub>2</sub> (♀) × diploid red crucian carp (♂), and F<sub>2</sub> (♀) × F<sub>10</sub> tetraploid (♂). The ploidy levels of the progeny of the two crosses were examined by chromosome counting and DNA content measurement by flow cytometer. In the offspring of the former cross, tetraploids, trip-loids, and diploid were obtained. In the progeny of the latter cross, tetraploids and triploids were observed. The production of the different ploidy level fish in the progeny of the two crosses provided a further evidence that F<sub>2</sub> might generate triploid, diploid and haploid eggs. The presence of the male tetraploid found in F<sub>2</sub> (♀) × diploid red crucian carp (♂) suggested that the genotype of XXXY probably existed in the tetraploid progeny. The gonadal structures of the tetraploids and triploids indicated that both female and male tetraploids were fertile and the triploids were sterile. We concluded that the formations of different ploidy level eggs from F<sub>2</sub> were contributed by endoreduplication and fusion of germ cells.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60055-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91983587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Molecular Cloning and Preliminary Function Study of a Novel Human Gene, TSARG7, Related to Spermatogenesis 人类精子发生相关基因TSARG7的分子克隆及功能初步研究

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60054-1

TAN Xiao-Jun , HUANG Zhi-Ping , LI Lu-Yun , NIE Dong-Song , ZHONG Chang-Gao , FU Jun-Jiang , LU Guang-Xiu

{"title":"Molecular Cloning and Preliminary Function Study of a Novel Human Gene, TSARG7, Related to Spermatogenesis","authors":"TAN Xiao-Jun , HUANG Zhi-Ping , LI Lu-Yun , NIE Dong-Song , ZHONG Chang-Gao , FU Jun-Jiang , LU Guang-Xiu","doi":"10.1016/S0379-4172(06)60054-1","DOIUrl":"10.1016/S0379-4172(06)60054-1","url":null,"abstract":"<div><p>A novel human gene <em>TSARG7</em> (GenBank accession No. AY513610) was identified from a human testis cDNA library by using the m <em>TSARG7</em> gene (GenBank accession No. AY489184) as an electronic probe. The gene whose full cDNA length is 2 463 bp containing 12 exons and 11 introns is located in the human chromosome 8p11.21. The predicted protein encoded by this gene contains 456 amino acids with a theoretical molecular weight of 56 295 dalton and isoelectric point of 9.13. It is a new member of the acyltransferase family since its sequence possesses the highly conserved PlsC domain existing in all acyltransferase-like proteins. Two groups, the <em>TSARG7</em> and m<em>TSARG7</em>, the <em>TSARG7</em> and Au041707, share 97% identity in the 456 amino acids. Expression of the <em>TSARG7</em> gene is restricted to the testis. Subcellular localization studies show that the EGFP-tagged <em>TSARG7</em> protein was localized in the cytoplasm of GC-1 cells. The <em>TSARG7</em> mRNA expression was initiated in the testis of a 13-year-old boy, and its level increased steadily along with spermatogenesis and sexual maturation of the human. The results of heat stress experiment demonstrate that <em>TSARG7</em> expression has a relation with temperature. In conclusion, our study suggests that we have cloned a novel human gene and this gene may play an important role in human spermatogenesis and sexual maturation.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60054-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

RFLP Analysis for Mitochondrial Genome of CMS-Rice cms水稻线粒体基因组的RFLP分析

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60058-9

HUANG Wei , WANG Li , YI Ping , TAN Xue-Lin , ZHANG Xue-Mei , ZHANG Zai-Jun , LI Yang-Sheng , ZHU Ying-Guo

引用次数: 8

Research Advances on Transgenic Plant Vaccines 转基因植物疫苗的研究进展

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60053-X

HAN Mei , SU Tao , ZU Yuan-Gang , AN Zhi-Gang

{"title":"Research Advances on Transgenic Plant Vaccines","authors":"HAN Mei , SU Tao , ZU Yuan-Gang , AN Zhi-Gang","doi":"10.1016/S0379-4172(06)60053-X","DOIUrl":"10.1016/S0379-4172(06)60053-X","url":null,"abstract":"<div><p>In recent years, with the development of genetics molecular biology and plant biotechnology, the vaccination (e.g. genetic engineering subunit vaccine, living vector vaccine, nucleic acid vaccine) programs are taking on a prosperous evolvement. In particular, the technology of the use of transgenic plants to produce human or animal therapeutic vaccines receives increasing attention. Expressing vaccine candidates in vegetables and fruits open up a new avenue for producing oral/edible vaccines. Transgenic plant vaccine disquisitions exhibit a tempting latent exploiting foreground. There are a lot of advantages for transgenic plant vaccines, such as low cost, easiness of storage, and convenient immune-inoculation. Some productions converged in edible tissues, so they can be consumed directly without isolation and purification. Up to now, many transgenic plant vaccine productions have been investigated and developed. In this review, recent advances on plant-derived recombinant protein expression systems, infectious targets, and delivery systems are presented. Some issues of high concern such as biosafety and public health are also discussed. Special attention is given to the prospects and limitations on transgenic plant vaccines.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60053-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25981924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Changes in Variance Components of Flanking Marker Genotypes Under Varying Selection Intensities 不同选择强度下侧翼标记基因型方差组成的变化

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60056-5

WANG Hui , CHEN Yao-Sheng

{"title":"Changes in Variance Components of Flanking Marker Genotypes Under Varying Selection Intensities","authors":"WANG Hui , CHEN Yao-Sheng","doi":"10.1016/S0379-4172(06)60056-5","DOIUrl":"10.1016/S0379-4172(06)60056-5","url":null,"abstract":"<div><p>Selection is practically ubiquitous during marker-QTL linkage analysis with an experimental population. Thus, it is necessary to investigate the impacts of selection upon linkage analyses in order to obtain unbiased estimates of QTL position and effect. In this article, by exploiting flanking markers through the widely applied half-sib design, we have developed the structures of three variance components, i.e., variance component between marker genotypes, polygenic variance component and recombinant variance component within marker genotypes. Changes in these variance components under varying selection intensities were investigated in this study to formulate the effects of selection on various variance components. Results showed clearly that all variance components presented were quite sensitive to changes in selection intensity. As selection intensity increased, all variance components declined by differing extents in a quadratic fashion. Comparatively speaking, the variance between marker genotypes decreased most drastically, followed by the polygenic variance within marker genotypes and then the recombinant variance within marker genotypes, which suggested a decrease of power for QTL linkage analysis. Therefore, steps should be taken to avoid as much as possible the presence of selection in real populations, so as to further eliminate the negative effects of selection on QTL linkage analysis.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60056-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Analysis and Molecular Tagging on a Novel Excessive Tillering Mutant in Rice 水稻一个新的多分蘖突变体的遗传分析与分子标记

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60059-0

JIANG Zhao-Xue , WANG Shi-Quan , DENG Qi-Ming , HE Ti-Hong , LI Ping

{"title":"Genetic Analysis and Molecular Tagging on a Novel Excessive Tillering Mutant in Rice","authors":"JIANG Zhao-Xue , WANG Shi-Quan , DENG Qi-Ming , HE Ti-Hong , LI Ping","doi":"10.1016/S0379-4172(06)60059-0","DOIUrl":"10.1016/S0379-4172(06)60059-0","url":null,"abstract":"<div><p>A rice (<em>Oryza sativa</em> L.) mutant with an excessive tiller number, designated ext-M1B, was found in the F<sub>2</sub> progenies generated from the cross between M1B and GMS-1 (a genetic male sterile), whose number of tillers was 121. The excessive tillering mutant also resulted in significant changes in plant height, flag leaf, stem, filled grains per panicle, and productive panicles per plant. The inbreeding progenies of ext-M1B exhibited the same mutant phenotype. The crosses from ext-M1B/M1B, M1B/ext-M1B, 2480B/ext-M1B, D62B/ext-M1B, G46B/ext-M1B, and G683B/ext-M1B expressed normal tillering in F<sub>1</sub>, and segregated into two different phenotypes of normal tillering type and excessive tillering type in a ratio of 3:1 in F<sub>2</sub>. Inheritance analysis indicated that the excessive tillering character was controlled by a single recessive nucleic gene. By BSA (bulked segregants analysis) and microsatellite makers with the F<sub>2</sub> population of 2480B/ext-M1B as the mapping population, RM197, RM584, and RM225, all of which were located on the short arm of rice chromosome 6, were identified to be linked with the excessive tillering gene with genetic distance of 3.8 cM, 5.1 cM, and 5.2 cM, respectively. This gene is probably a new excessive tillering gene in rice and is designated tentatively <em>ext</em>-M1B (t).</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60059-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Analysis of Microsatellites in Citrus Unigenes 柑橘属植物微卫星分析

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60060-7

JIANG Dong, ZHONG Guang-Yan, HONG Qi-Bing

{"title":"Analysis of Microsatellites in Citrus Unigenes","authors":"JIANG Dong, ZHONG Guang-Yan, HONG Qi-Bing","doi":"10.1016/S0379-4172(06)60060-7","DOIUrl":"10.1016/S0379-4172(06)60060-7","url":null,"abstract":"<div><p>Simple sequence repeats (SSRs) were investigated in the unigene sequences from expressed sequence tags (EST) of sweet orange (<em>Citrus sinensis osbeck</em>), trifoliate orange (<em>Poncirus trifoliata Raf.</em>) and other citrus species and cultivars. A total of 37 802 citrus unigene sequences corresponding to 23.29 Mb were searched, resulting in the identification of 8 218 SSRs. Among them there were 4 913 (59.8%) mono-, 1 419 (17.3%) di-, 1 709 (20.8%) tri-, 114 (1.39%) tetra-, 23 (0.28%) penta- and 40 (0.49%) hexa-nucleotide SSRs. The estimated frequency of SSRs was approximately 1/2.8 kb, which could be extrapolated to 1 SSR-containing unigene in 4.6 unigenes. The maximum length of the SSR ranged from 40 to 105 bp depending on the repeating numbers of the motif in the SSR. The overall average length of SSRs was 20.9 bp. The frequencies of different SSR types (di-, tri-, tetra-, and penta-nucleotide repeats) were very similar between sweet orange and trifoliate orange. The mononucelotide repeats appeared to be the most abundant SSRs within sweet orange and trifoliate orange, followed by trimeric repeats. The adenine rich repeats such as A/T, AG, AT, AAG, AAAT, AAAG, AAAT, AAAAG, AAAAT etc. were predominant in each type of SSRs (mono-, di-, tri-, tetra-, and penta-), whereas the C/G, CG, CCG repeats were less abundant. Twenty-five primer pairs flanking EST-SSR loci were designed to detect the possible polymorphism of six citrus cultivars including sweet orange and trifoliate orange. The PCR result with all these 25 primer pairs revealed the existence of polymorphism within six citrus cultivars confirming that citrus EST database could be efficiently exploited for the development of gene-derived SSR markers.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60060-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Polymorphism of SARS-CoV Genomes sars冠状病毒基因组多态性研究

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60061-9

SHANG Lei , QI Yan , BAO Qi-Yu , TIAN Wei , XU Jian-Cheng , FENG Ming-Guang , YANG Huan-Ming

{"title":"Polymorphism of SARS-CoV Genomes","authors":"SHANG Lei , QI Yan , BAO Qi-Yu , TIAN Wei , XU Jian-Cheng , FENG Ming-Guang , YANG Huan-Ming","doi":"10.1016/S0379-4172(06)60061-9","DOIUrl":"10.1016/S0379-4172(06)60061-9","url":null,"abstract":"<div><p>In this work, severe acute respiratory syndrome associated <em>coronavirus</em> (SARS-CoV) genome BJ202 (AY864806) was completely sequenced. The genome was directly accessed from the stool sample of a patient in Beijing. Comparative genomics methods were used to analyze the sequence variations of 116 SARS-CoV genomes (including BJ202) available in the NCBI Gen-Bank. With the genome sequence of GZ02 as the reference, there were 41 polymorphic sites identified in BJ202 and a total of 278 polymorphic sites present in at least two of the 116 genomes. The distribution of the polymorphic sites was biased over the whole genome. Nearly half of the variations (50.4%, 140/278) clustered in the one third of the whole genome at the 3′ end (19.0 kb-29.7 kb). Regions encoding Orf10–11, Orf3/4, E, M and S protein had the highest mutation rates. A total of 15 PCR products (about 6.0 kb of the genome) including 11 fragments containing 12 known polymorphic sites and 4 fragments without identified polymorphic sites were cloned and sequenced. Results showed that 3 unique polymorphic sites of BJ202 (positions 13 804, 15 031 and 20 792) along with 3 other polymorphic sites (26 428, 26 477 and 27 243) all contained 2 kinds of nucleotides. It is interesting to find that position 18379 which has not been identified to be polymorphic in any of the other 115 published SARS-CoV genomes is actually a polymorphic site. The nucleotide composition of this site is A (8) to G (6). Among 116 SARS-CoV genomes, 18 types of deletions and 2 insertions were identified. Most of them were related to a 300 bp region (27 700–28 000) which encodes parts of the putative ORF9 and ORF10–11. A phylogenetic tree illustrating the divergence of whole BJ202 genome from 115 other completely sequenced SARS-CoVs was also constructed. BJ202 was phylogeneticly closer to BJ01 and LLJ-2004.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60061-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5