Acta Genetica Sinica最新文献_第4页

Molecular Characterization of a HMW Glutenin Subunit Allele Providing Evidence for Silencing of x-type Gene on Glu-B1 一个HMW谷蛋白亚基等位基因的分子特征为Glu-B1上x型基因的沉默提供证据

Acta Genetica Sinica Pub Date : 2006-10-01 DOI: 10.1016/S0379-4172(06)60127-3

YANG Zu-Jun , LI Guang-Rong , LIU Chang , FENG Juan , ZHOU Jian-Ping , REN Zheng-Long

{"title":"Molecular Characterization of a HMW Glutenin Subunit Allele Providing Evidence for Silencing of x-type Gene on Glu-B1","authors":"YANG Zu-Jun , LI Guang-Rong , LIU Chang , FENG Juan , ZHOU Jian-Ping , REN Zheng-Long","doi":"10.1016/S0379-4172(06)60127-3","DOIUrl":"10.1016/S0379-4172(06)60127-3","url":null,"abstract":"<div>Understanding the molecular structure of high-molecular-weight glutenin subunit (HMW-GS) may provide useful evidence for the study on the improvement of quality of cultivated wheat and the evolution of Glu-1 alleles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) shows that the subunits encoded by Glu-B1 were null, named 1Bxm, in a Triticum turgidum var. dicoccoides line PI94640. Primers based on the conserved regions in wheat HMW-GS gene promoter and coding sequences were used to amplify the genomic DNA of line PI94640. The PCR products were sequenced, and the total nucleotide sequence of 3 442 bp including upstream sequence of 1 070 bp was obtained. Compared with the reported gene sequences of Glu-1Bx alleles, the promoter region of the Glu-1Bxm showed close resemblance to 1Bx7. The Glu-1Bxm coding region differs from the other Glu-1Bx alleles for a deduced mature protein with only 212 residues, and a stop codon (TAA) at 637 bp downstream from the start codon was present, which was probably responsible for the silencing of x-type subunit genes at the Glu-B1 locus. Phylogenetic tree based on the nucleotide sequence alignment of HMW glutenin subunit genes showed that 1Bxm was the most ancient type of Glu-B1 alleles, suggesting that the evolution rates are different among Glu-1Bx genes. Further study on the contribution of the unique silenced Glu-B1 alleles to quality improvement was also discussed.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 929-936"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60127-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26314381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Effect of Genetic Variations of the POU1F1 Gene on Growth Traits of Nanyang Cattle POU1F1基因遗传变异对南阳牛生长性状的影响

Acta Genetica Sinica Pub Date : 2006-10-01 DOI: 10.1016/S0379-4172(06)60124-8

XUE Kai , CHEN Hong , WANG Shan , CAI Xin , LIU Bo , ZHANG Cun-Fang , LEI Chu-Zhao , WANG Xin-Zhuang , WANG Yi-Min , NIU Hui

{"title":"Effect of Genetic Variations of the POU1F1 Gene on Growth Traits of Nanyang Cattle","authors":"XUE Kai , CHEN Hong , WANG Shan , CAI Xin , LIU Bo , ZHANG Cun-Fang , LEI Chu-Zhao , WANG Xin-Zhuang , WANG Yi-Min , NIU Hui","doi":"10.1016/S0379-4172(06)60124-8","DOIUrl":"10.1016/S0379-4172(06)60124-8","url":null,"abstract":"<div>PCR-RFLP was applied to analyze the effect of the genetic variations of the POU1F1 gene on growth traits of 100 Nanyang cattle. The results showed that the 451 bp PCR product digested with Hinf I demonstrated polymorphism in the population, which was at Hardy-Weinberg equilibrium. Moreover, the frequencies of alleles A/B in the Nanyang population were 0.465/0.535. The association of the variations of the POU1F1 gene with the growth traits in the population was analyzed. The following parameters were greater in individuals with a genotype BB than in those with an genotype AB: birth weight, average weight increase before ablactation, body height at 12 months, body weight, body length, and chest girth at 6 months and 12 months (P<0.05). The body weight at 12 months was higher in the BB individuals than in the AA individuals (P <0.05). The body weight and body sizes also showed a trend of allele B> allele A in the other age groups. Therefore, the genotype BB maybe a dominant genotype and the allele B may be a dominant allele. These results imply that the allele B of the POU1F1 gene is likely to positively affect the growth traits.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 901-907"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60124-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26313890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Cloning and Characterization of Four B-hordein Genes from Tibetan Hull-less Barley (Hordeum vulgare subsp. vulgare) 西藏无壳大麦(Hordeum vulgare subsp) 4个b -苦苷基因的克隆与特性分析。)

Acta Genetica Sinica Pub Date : 2006-10-01 DOI: 10.1016/S0379-4172(06)60128-5

HAN Zhao-Xue , QIAN Gang , PAN Zhi-Fen , DENG Guang-Bing , WU Fang , TANG Ya-Wei , QIANG Xiao-Lin , YU Mao-Qun

{"title":"Cloning and Characterization of Four B-hordein Genes from Tibetan Hull-less Barley (Hordeum vulgare subsp. vulgare)","authors":"HAN Zhao-Xue , QIAN Gang , PAN Zhi-Fen , DENG Guang-Bing , WU Fang , TANG Ya-Wei , QIANG Xiao-Lin , YU Mao-Qun","doi":"10.1016/S0379-4172(06)60128-5","DOIUrl":"10.1016/S0379-4172(06)60128-5","url":null,"abstract":"<div>Four B-hordein genes, designated BH1-BH4, were cloned using PCR amplification from two hull-less barley cultivars, ZQ7239 and ZQ148, collected from Tibet. The results of sequencing indicated that BH1-BH4 contained complete open reading frames (ORFs). Comparison of their predicted polypeptide sequences with the published sequences suggested that they all share the same basic protein structure. Phylogenetic analysis indicated that the deduced amino-acid sequences of BH1-BH4 genes were more closely related to B-hordeins from cultivated barley (Hordeum vulgare L.) than to any other prolamins from wild barley and Aegilops tauschii. Comparison of the coding regions of BH1-BH4 genes showed that BH1 had a lower sequence identity to other previously published B-hordeins than the other three B-hordeins obtained in this study. BH1 was then cloned in a bacterial expression vector based on bacteriophage T7 RNA polymerase. The resulting plasmid produced a 28.15 kDa protein in Escherichia coli. The potential value of B-hordein genes in grain quality improvement of hull-less barley has been discussed.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 10","pages":"Pages 937-947"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60128-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26314382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Structure of the Mitochondrial DNA Control Region of the Sinipercine Fishes and Their Phylogenetic Relationship 鳜类鱼类线粒体DNA控制区结构及其系统发育关系

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60112-1

ZHAO Jin-Liang, WANG Wei-Wei, LI Si-Fa, CAI Wan-Qi

{"title":"Structure of the Mitochondrial DNA Control Region of the Sinipercine Fishes and Their Phylogenetic Relationship","authors":"ZHAO Jin-Liang, WANG Wei-Wei, LI Si-Fa, CAI Wan-Qi","doi":"10.1016/S0379-4172(06)60112-1","DOIUrl":"10.1016/S0379-4172(06)60112-1","url":null,"abstract":"<div>The mitochondrial DNA control region of Siniperca chuatsi, S. kneri, S. scherzeri, S. obscura, S. undulata, Coreosiniperca roulei and Coreoperca whiteheadi were amplified by PCR amplification and directly sequenced. The mtDNA control region of the sinipercine fishes could be separated into three domains, namely, the terminal associated sequence domain, the central conserved sequence domain and the conserved sequence block domain. The extended terminal associated sequence (ETAS), three conserved sequence blocks (CSB-F, CSB-E, CSB-D) in the central conserved sequence domain and three conserved sequence blocks (CSB1, CSB2, CSB3) in the conserved sequence block domain were also identified. The phylogenetic relationships among these sinipercine fishes were constructed through neighbor-joining and maximum parsimony methods using Percidae and Serranidae as outgroups. Results showed that sinipercine fishes were a monophyletic group, with Siniperca forming one group, and Coreoperca forming another group. Coreosiniperca roulei did not form an independent group but was merged into the genus Siniperca. Thus it should be renamed as Siniperca roulei.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 9","pages":"Pages 793-799"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60112-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26315243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

RFLP and AFLP Analysis of Inter- and Intraspecific Variation of Brassica rapa and B. napus Shows that B. rapa Is an Important Genetic Resource for B. napus Improvement 油菜和甘蓝型油菜种间和种内变异的RFLP和AFLP分析表明，甘蓝型油菜是甘蓝型油菜改良的重要遗传资源

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60115-7

LIU Ren-Hu , MENG Jin-Ling

{"title":"RFLP and AFLP Analysis of Inter- and Intraspecific Variation of Brassica rapa and B. napus Shows that B. rapa Is an Important Genetic Resource for B. napus Improvement","authors":"LIU Ren-Hu , MENG Jin-Ling","doi":"10.1016/S0379-4172(06)60115-7","DOIUrl":"10.1016/S0379-4172(06)60115-7","url":null,"abstract":"<div>Fingerprinting of 29 accessions of oilseed rape, including seven accessions of Brassica napus, and 22 accessions of B. rapa (B. campestris) from Europe, North America, and China was analyzed using RFLP and AFLP markers. In total, 1 477 polymorphic RFLP bands and 183 polymorphic AFLP bands from 166 enzyme-probe combinations and two pairs of AFLP primers, respectively, were scored for the 29 accessions. On average, RFLP analysis showed that the Arabidopsis EST probe detected more polymorphic bands in Brassica than the random genomic probe performed. More polymorphic RFLP markers were detected with the digestion of EcoR I or BamH I than HindIII. According to the number of bands amplified from each accession, the copy numbers of each gene in the genomes of B. rapa and B. napus were estimated. The average copy numbers in B. rapa of China, B. rapa of Europe, and B. napus, were 3.2, 3.1, and 2.9, respectively. Genetic distance based on the AFLP data was well correlated with that based on the RFLP data (r = 0.72, P<0.001), but 0.39 smaller on average. Genetic diversity analysis showed that Chinese B. rapa was more polymorphic than Chinese B. napus and European materials. Some European B. napus accessions were clustered into European B. rapa, which were distinctly different from Chinese B. napus. The larger variations of Chinese accessions of B. rapa suggest that they are valuable in oilseed rape breeding. Novel strategies to use intersubgenomic heterosis between genome of B. rapa (ArAr) and genome of B. napus (AnAnCnCn) were elucidated.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 9","pages":"Pages 814-823"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60115-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26256803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Construction of Fingerprinting and Genetic Diversity of Mulberry Cultivars in China by ISSR Markers 利用ISSR标记构建中国桑树品种指纹图谱及遗传多样性

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60119-4

ZHAO Wei-Guo , MIAO Xue-Xia , ZANG Bo , ZHANG Lin , PAN Yi-Le , HUANG Yong-Ping

引用次数: 12

Linkage and Association Between CA Repeat Polymorphism of the TNFR2 Gene and Obesity Phenotypes in Two Independent Caucasian Populations 两个独立高加索人群中TNFR2基因CA重复多态性与肥胖表型的连锁和关联

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60110-8

HUANG Qing-Yang , SHEN Hui , DENG Hong-Yi , Theresa Conway , Leo Elze , K. Michael Davies , Robert R. Recker , DENG Hong-Wen

{"title":"Linkage and Association Between CA Repeat Polymorphism of the TNFR2 Gene and Obesity Phenotypes in Two Independent Caucasian Populations","authors":"HUANG Qing-Yang , SHEN Hui , DENG Hong-Yi , Theresa Conway , Leo Elze , K. Michael Davies , Robert R. Recker , DENG Hong-Wen","doi":"10.1016/S0379-4172(06)60110-8","DOIUrl":"10.1016/S0379-4172(06)60110-8","url":null,"abstract":"<div>Previously, our group has reported a suggestive linkage evidence of 1p36 with body mass index (BMI) (LOD = 2.09). The tumor necrosis factor receptor 2 (TNFR2) at 1p36 is an excellent positional and functional candidate gene for obesity. In this study, we have investigated the linkage and association between the TNFR2 gene and obesity phenotypes in two large independent samples, using the quantitative transmission disequilibrium tests (QTDT). The first group was made up of 1 836 individuals from 79 multi-generation pedigrees. The second group was a randomly ascertained set of 636 individuals from 157 US Caucasian nuclear families. Obesity phenotypes tested include BMI, fat mass, and percentage fat mass (PFM). A significant result (P = 0.0056) was observed for linkage with BMI in the sample of the multigenerational pedigrees. Our data support the TNFR2 gene as a quantitative trait locus (QTL) underlying BMI variation in the Caucasian populations.</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 9","pages":"Pages 775-781"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60110-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26315241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Tissue Distribution and Developmental Changes of ghrelin mRNA Expression in Sheep 绵羊胃饥饿素mRNA表达的组织分布及发育变化

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60114-5

HUANG Zhi-Guo , XIONG Li , LIU Zhen-Shan , QIAO Yong , DAI Rong , XIE Zhuang , LIU Shou-Ren , SHI Guo-Qing , LIU Guo-Qing

{"title":"The Tissue Distribution and Developmental Changes of ghrelin mRNA Expression in Sheep","authors":"HUANG Zhi-Guo , XIONG Li , LIU Zhen-Shan , QIAO Yong , DAI Rong , XIE Zhuang , LIU Shou-Ren , SHI Guo-Qing , LIU Guo-Qing","doi":"10.1016/S0379-4172(06)60114-5","DOIUrl":"10.1016/S0379-4172(06)60114-5","url":null,"abstract":"<div>Male Kazak sheep and Xinjiang fine wool sheep, six for each different age group (days 2, 30, 60, 90 and 120), were used in the present study to investigate the tissue distribution and developmental changes of ghrelin mRNA expression in abomasum; however, there was no 120-day-old Kazak sheep. After measurement of body weight, the tissues such as hypothalamus, pituitary, heart, liver, rumen, reticulum, omasum, abomasum, duodenum, and longissimus dorsi muscle were sampled. And the total RNA of different tissues was extracted to determine the abundance of ghrelin mRNA by RT-PCR and real-time PCR. The results showed that (1) for both breeds, body weight among different ages was significantly different (P<0.05). And from day 30 to 90, the body weight of Kazak was significantly higher than that of Xinjiang (P<0.01); (2) Ghrelin mRNA existed in all the above tissues and was significantly higher in the abomasum than in other tissues (P<0.05); (3) the temporal patterns of abomasum ghrelin mRNA expression in Kazak and Xinjiang were similar. From day 2 to 60 in Kazak and 2 to 90 in Xinjiang, there was a steady increase in the ghrelin mRNA level. By day 60 in Kazak and day 90 in Xinjiang, the level reached a plateau and remained steady. These results also demonstrated that from birth to day 90, ghrelin mRNA level was significantly higher in Kazak than in Xinjiang (P<0.01).</div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 9","pages":"Pages 808-813"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60114-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26256802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Progress in Plant CACTA Elements 植物CACTA元件研究进展

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60109-1

TIAN Ping-Fang

引用次数: 13

Salt Induces Expression of RH3.2A, Encoding an H3.2-type Histone H3 Protein in Rice (Oryza sativa L.) 盐诱导水稻h3.2型组蛋白H3蛋白RH3.2A的表达

Acta Genetica Sinica Pub Date : 2006-09-01 DOI: 10.1016/S0379-4172(06)60117-0

QIU Sheng-Ping, HUANG Ji, PAN Li-Juan, WANG Mei-Mei, ZHANG Hong-Sheng

引用次数: 6