Analysis of the Genetic Diversity and the Phylogenetic Evolution of Chinese Sheep Based on Cyt b Gene Sequences

WANG Xin , MA Yue-Hui , CHEN Hong
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引用次数: 14

Abstract

The complete sequences of Cyt b gene from 20 individuals belonging to eight Chinese indigenous sheep breeds and one foreign breed were studied. The results showed that the hapolotype diversity of Chinese sheep breeds was 97.1%. The mean nucleotide composition of all the sequences was 27.1% T, 28.5% C, 31.4% A, and 13.0% G. The nucleotide diversity was 0.602%. A total of 43 mutation sites were detected, including 40 transitions and 3 transversions. Fu's test of selective neutrality showed that the sheep populations had no population demographic expansion (0.10 > P > 0.05). The different clustering methods, namely neighbor-joining, minimum evolution, and unweighted pair group method with arithmetic means, all showed a similar result, which indicated that Chinese local sheep had three maternal resources.

基于Cyt b基因序列的中国绵羊遗传多样性及系统进化分析
对中国8个地方羊品种和1个外源羊品种20只羊的Cyt b基因全序列进行了研究。结果表明,中国绵羊品种的单倍型多样性为97.1%。所有序列的平均核苷酸组成分别为27.1% T、28.5% C、31.4% A和13.0% g,核苷酸多样性为0.602%。共检测到43个突变位点,包括40个过渡位点和3个翻转位点。Fu's选择性中性检验表明,绵羊种群没有种群人口扩张(0.10 >P比;0.05)。不同的聚类方法,即邻居加入法、最小进化法和带算术平均数的非加权对群聚类结果相似,表明中国地方羊有三个母系资源。
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