{"title":"Mutator Transposon in Maize and MULEs in the Plant Genome","authors":"DIAO Xian-Min , Lisch Damon","doi":"10.1016/S0379-4172(06)60075-9","DOIUrl":"10.1016/S0379-4172(06)60075-9","url":null,"abstract":"<div><p><em>Mutator (Mu)</em> is by far the most mutagenic plant transposon. The high frequency of transposition and the tendency to insert into low copy sequences for such transposon have made it the primary means by which genes are mutagenized in maize (<em>Zea mays</em> L.). <em>Mus</em> like elements (<em>MULEs</em>) are widespread among angiosperms and multiple-diverged functional variants can be present in a single genome. <em>MULEs</em> often capture genetic sequences. These <em>Pack-MuLEs</em> can mobilize thousands of gene fragments, which may have had a significant impact on host genome evolution. There is also evidence that <em>MULEs</em> can move between reproductively isolated species. Here we present an overview of the discovery, features and utility of <em>Mu</em> transposon. Classification of <em>Mu</em> elements and future directions of related research are also discussed. Understanding <em>Mu</em> will help us elucidate the dynamic genome.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 477-487"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60075-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation Analysis Between Single-Nucleotide Polymorphism of Malate Dehydrogenase Gene 5′-Flanking Region and Growth and Body Composition Traits in Chicken","authors":"GUAN Hong-Ying, TANG Zhi-Quan, LI Hui","doi":"10.1016/S0379-4172(06)60078-4","DOIUrl":"10.1016/S0379-4172(06)60078-4","url":null,"abstract":"<div><p>Malate dehydrogenase (MD) is a key enzyme that plays an important role in energy metabolism. It catalyzes the oxidative decarboxylation of L-malate to yield CO<sub>2</sub> and pyruvate, while simultaneously generating NADPH from NADP<sup>+</sup>. The NADPH generated can be utilized in <em>de novo</em> synthesis of palmitate, which is the precursor molecule for the formation of other long-chain fatty acids. And high levels of MD will also activate muscle development. The current study was designed to investigate the effects of <em>MD</em> gene on growth and body-composition traits in chicken. The eighth generation population of Northeast Agricultural University broiler lines divergently selected for its abdominal fat and Northeast Agricultural University F<sub>2</sub> resource population were used in the research. Polymorphisms were detected by DNA sequencing and PCR-RFLP method was then developed to screen the population. A single mutation at the position of the 235 bp (Accession No. U49693) of <em>MD</em> 5′-flanking region was found. The correlation analysis between the polymorphism of the <em>MD</em> gene and growth and body composition traits was carried out using the appropriate statistic model. Least-square analysis showed that the <em>BB</em> genotype birds had much higher pectoralis major weight and percentage of pectoralis major than <em>AA</em> genotype birds (<em>P</em><0.05). The abdominal fat weight, percentage of abdominal fat, the liver weight and percentage of liver weight of the <em>AA</em> genotype birds were much higher than those of <em>BB</em> genotype birds (<em>P</em><0.05). These results indicate that <em>MD</em> gene is the major gene or is linked to the major gene that affects the growth and body composition traits in chicken.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 501-506"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60078-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HUANG Zhi-Guo , XIONG Li , LIU Zhen-Shan , QIAO Yong , LIU Shou-Ren , REN Hang-Xing , XIE Zhuang , LIU Guo-Qing , LI Xue-Bin
{"title":"The Developmental Changes and Effect on IMF Content of H-FABP and PPARγ mRNA Expression in Sheep Muscle","authors":"HUANG Zhi-Guo , XIONG Li , LIU Zhen-Shan , QIAO Yong , LIU Shou-Ren , REN Hang-Xing , XIE Zhuang , LIU Guo-Qing , LI Xue-Bin","doi":"10.1016/S0379-4172(06)60079-6","DOIUrl":"10.1016/S0379-4172(06)60079-6","url":null,"abstract":"<div><p>Male Kazak sheep and Xinjiang fine wool sheep of different ages were selected to investigate the developmental changes and effect on intramuscular fat (IMF) content of heart fatty acid-binding protein (<em>H-FABP</em>) and peroxisome proliferator-activated receptor γ (<em>PPAR</em>γ) mRNA expression in muscle. Longissimus dorsal muscle was sampled to measure IMF content; and total RNA was extracted to determine <em>H-FABP</em> and <em>PPAR</em>γ mRNA expression levels by real-time PCR. The results showed that: (1) The IMF content increased continuously with growing and showed significant differences (<em>P</em><0.05) between ages in male Kazak sheep, but no such differences (<em>P</em>>0.05) existed in Xinjiang fine wool sheep. Furthermore, the IMF content in Kazak sheep was very much higher (<em>P</em><0.01) than that of the other breed from day 30 to 90; (2) <em>H-FABP</em> mRNA expression level was the highest on day 2 and showed significant differences (<em>P</em><0.05) between ages in male Kazak sheep as well as in Xinjiang fine wool sheep. In the former breed, the expression reached the lowest point at day 30, and then rose continuously. But in the latter breed, it declined continuously from day 2 to 90, and then increased; (3) Significant differences (<em>P</em><0.05) of <em>PPAR</em>γ mRNA expression between ages occurred in both breeds. In male Kazak sheep, <em>PPAR</em>γ mRNA expression declined from day 2 to 90, while in the other breed it increased continuously from day 2 to 60, but reached the lowest level at day 90, then increased; (4) In male Kazak sheep, the mRNA expression level of <em>H-FABP</em> was highly positively correlated (<em>r</em>=0.737, <em>P</em><0.01) with IMF content from day 30 to 90, but that of <em>PPAR</em>γ was highly negatively correlated (<em>r</em>=−0.835, <em>P</em><0.01) with IMF content from day 2 to 90.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 507-514"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60079-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association Between a Functional COMT Polymorphism, Mental Retardation and Cognition in Qinba Area Children","authors":"ZHANG Ke-Jin , GAO Xiao-Cai , LI Rui-Lin , CHEN Chao , ZHENG Zi-Jian , HUANG Shao-Ping , ZHANG Fu-Chang","doi":"10.1016/S0379-4172(06)60077-2","DOIUrl":"10.1016/S0379-4172(06)60077-2","url":null,"abstract":"<div><p>Catechol-O-methyl transferase (COMT) plays an important role in the metabolism of neurotransmitters. Two alleles of the <em>COMT</em> gene as a result of a G/A transition in the exon 4 can lead to different <em>COMT</em> enzymatic activities. Much genetic research has revealed that this <em>COMT</em> functional polymorphism was related to human psychiatric disorders. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods were used to discern the relationships among the functional polymorphism of <em>COMT</em>, mental retardation (MR), and general cognitive ability of children. The results of the case-control analysis showed that there was no association between the frequencies of genotypes of <em>COMT</em> and MR (<em>x</em><sup>2</sup>=0.776, <em>P</em>>0.05) or between the frequency of <em>COMT</em> alleles and MR (<em>x</em><sup>2</sup>=0.335, <em>P</em>>0.05). <em>COMT</em> polymorphism was found in children whose intelligence quotient (IQ) was above 55. In normal children (IQ≥85), the frequencies of high-activity allele <em>COMT</em><sup>H</sup> and the homozygote genotype <em>COMT</em><sup>HH</sup> were 60.98% and 79.28%, respectively. Both were higher than those of the borderline group (46.67% and 70.67%, 0.10 > <em>P</em>>0.05). Therefore, the result of this study suggests that this functional polymorphism is not an important risk factor for MR, but the <em>COMT<sup>HH</sup></em> genotype may have a positive effect on cognitive performance in normal children in the Qinba area.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 495-500"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60077-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
YU Xin-Qiao, MEI Han-Wei, LUO Li-Jun, LIU Guo-Lan, LIU Hong-Yan, ZOU Gui-Hua, HU Song-Ping, LI Ming-Sou, WU Jin-Hong
{"title":"Dissection of Additive, Epistatic Effect and Q × E Interaction of Quantitative Trait Loci Influencing Stigma Exsertion Under Water Stress in Rice","authors":"YU Xin-Qiao, MEI Han-Wei, LUO Li-Jun, LIU Guo-Lan, LIU Hong-Yan, ZOU Gui-Hua, HU Song-Ping, LI Ming-Sou, WU Jin-Hong","doi":"10.1016/S0379-4172(06)60083-8","DOIUrl":"10.1016/S0379-4172(06)60083-8","url":null,"abstract":"<div><p>Four flowering related traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES), dual exserted stigma (PDES) and total exserted stigma (PES) of a RI population with 185 lines under water stress and non-stress conditions for 2 years, were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. Highest phenotypic correlation was found between PSES and PES (<em>r</em> = 0.9752<sup>***</sup>), followed by PDES and PES (<em>r</em> = 0.7150<sup>***</sup>), and PSES and PDES (<em>r</em> = 0.5424<sup>***</sup>). Based on a linkage map of 203 SSR markers, six main effect QTLs were detected for SNP and three or four main effect QTLs were associated with PSES, PDES and PES under stress or non-stress conditions. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%–9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES, PDES and PES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress condition, implying that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 542-550"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60083-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HAO Lin , SONG Guo-Li , LI Bing-Lin , WANG Kun-Bo , WANG Chun-Ying , LI Xin
{"title":"Karyotype Analysis of Gossypium arboreum × G. bickii by Genome in situ Hybridization","authors":"HAO Lin , SONG Guo-Li , LI Bing-Lin , WANG Kun-Bo , WANG Chun-Ying , LI Xin","doi":"10.1016/S0379-4172(06)60086-3","DOIUrl":"10.1016/S0379-4172(06)60086-3","url":null,"abstract":"<div><p>By using genome <em>in situ</em> hybridization (GISH) on root somatic chromosomes of allotetraploid derived from the cross <em>Gossypium arboreum</em> × <em>G. bickii</em> with genomic DNA (gDNA) of <em>G. bickii</em> as a probe, two sets of chromosomes, consisting of 26 chromosomes each, were easily distinguished from each other by their distinctive hybridization signals. GISH analysis directly proved that the hybrid <em>GarboreumxG. bickii</em> is an allotetraploid amphiploid. The karyotype formula of the species was 2n = 4x = 52 = 46m (4sat) + 6sm (4sat). We identified four pairs of satellites with two pairs in each sub-genome. FISH analysis using 45S rDNA as a probe showed that the cross <em>G. arboreumxG. bickii</em> contained 14 NORs. At least five pairs of chromosomes in the G sub-genome showed double hybridization (red and blue) in their long arms, which indicates that chromatin introgression from the A sub-genome had occurred.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 565-572"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60086-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HAN Lei , ZHANG Hong-Yu , XU Pei-Zhou , LI Yun , WANG Xu-Dong , WU Xian-Jun
{"title":"Genetic Analysis and Histological Study of Red Seed in Rice","authors":"HAN Lei , ZHANG Hong-Yu , XU Pei-Zhou , LI Yun , WANG Xu-Dong , WU Xian-Jun","doi":"10.1016/S0379-4172(06)60085-1","DOIUrl":"10.1016/S0379-4172(06)60085-1","url":null,"abstract":"<div><p>Reciprocal crosses between red and achromatic rice revealed that the seed color of F<sub>1</sub> was determined by its female parent. According to the seed color and plant segregation ratio of F<sub>1</sub>, F<sub>2</sub>, and F<sub>3</sub> generations, the red phenotype of red double-haploid seed was determined by a dominant, monogene with maternal effect. Histological study showed that the red pigments accumulated in the pericarp layer only. The assay of developmental timing of pigment accumulation showed that the red color accumulated from desiccation stage to perfectly maturation stage of the seeds.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 559-564"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60085-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship Among H-FABP Gene Polymorphism, Intramuscular Fat Content, and Adipocyte Lipid Droplet Content in Main Pig Breeds with Different Genotypes in Western China","authors":"PANG Wei-Jun, BAI Liang, YANG Gong-She","doi":"10.1016/S0379-4172(06)60080-2","DOIUrl":"10.1016/S0379-4172(06)60080-2","url":null,"abstract":"<div><p>H-FABP (Heart fatty acid-binding protein), a member of FABP family, plays an essential role in long-chain fatty acid uptake and metabolic homeostasis. Its role in pig intramuscular fat content remains poorly understood, especially in local pig breeds in western China. In this study, the genetic variations of 5′-upstream region and the second intron in porcine <em>H-FABP</em> gene were investigated by PCR-RFLP in 256 pigs including Duroc, Large White, Landrace, Neijiang, Rongchang, Bamei pig, Hanjiang Black, Hanzhong White, and the wild ones. The effect of <em>H-FABP</em> gene on the IMF content was analyzed by the least square method. Lipid droplet morphology and content in adipocytes cultured from pigs with different <em>H-FABP</em> genotypes, were studied by oil red O staining and a triglyceride assay kit. Results showed a <em>Hin</em>f I -RFLP in these eight pig breeds and wild pigs, among which Large white, Bamei pig, Hanjiang Black, Hanzhong White, and wild pigs presented with low polymorphism while the other breeds had intermediate polymorphism. There was no <em>Hae</em> III or <em>Msp</em> I -RFLPs in the four Chinese local pig breeds tested, but Duroc, Landrace, Large White, Hanzhong White and wild pig had polymorphism. Landrace, Large White and wild pigs had low levels of <em>Hae</em> III-and <em>Msp</em> I -RFLP, whereas others had intermediate polymorphism. <em>H-FABP</em> genotypes significantly affected the IMF content (<em>P</em><0.05). The IMF content ordered by <em>H-FABP</em> genotypes were <em>HH</em>><em>Hh</em>><em>hh</em>, <em>DD</em><<em>Dd</em><<em>dd</em>, and <em>AA</em><<em>Aa</em><<em>aa.</em> The genetic effect values were 3.89, 3.42, 3.17, 2.27, 2.49, 2.91, 2.28, 2.70, and 2.95, respectively. Fat deposition in adipocytes was stronger in the <em>HH</em>, <em>dd</em> and <em>aa</em> genotypes than in others. The results suggest that porcine meat quality may be improved by increasing the frequency of genotype <em>aa</em>-<em>dd</em>-<em>HH</em> in pig breeds.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 6","pages":"Pages 515-524"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60080-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26111067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TONG Han-Hua , MEI Han-Wei , YU Xin-Qiao , XU Xiao-Yan , LI Ming-Shou , ZHANG Shan-Qing , LUO Li-Jun
{"title":"Identification of Related QTLs at Late Developmental Stage in Rice (Oryza sativa L.) Under Two Nitrogen Levels","authors":"TONG Han-Hua , MEI Han-Wei , YU Xin-Qiao , XU Xiao-Yan , LI Ming-Shou , ZHANG Shan-Qing , LUO Li-Jun","doi":"10.1016/S0379-4172(06)60073-5","DOIUrl":"10.1016/S0379-4172(06)60073-5","url":null,"abstract":"<div><p>QTL underlying related traits at the late developmental stage under two different nitrogen levels were investigated in rice using a population of chromosome segment substitution lines (CSSL) derived from a cross between Teqing and Lemont. A total of 31 QTLs referring 5 traits, that is, plant height (PH), panicle number per plant (PN), chlorophyll content (CC), shoot dry weight (SDW) and grain yield per plant (YD), were detected. Under normal N level, 3 QTLs were detected for each trait, while under low N level, 5,4, 5 and 2 QTLs were detected for PH, PN, CC and SDW respectively. Most of the QTLs were located on chromosome 2, 3, 7, 11 and 12. QTLs controlling different traits or the same trait under different N levels were mapped on the same or adjacent intervals, forming several clusters in rice chromosomes. More than two traits were controlled by QTLs on one of four intervals (RM30-RM439, RM18-RM478, RM309-RM270, and RM235-RM17), suggesting that there were some pleiotropic effects. It was supposed that some QTLs only detected at low N level might be associated with the ability to tolerate the low N stress in rice.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 5","pages":"Pages 458-467"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60073-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26042087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FENG Yuan-Xi , BAI Jing , ZHANG Chun-Yu , FU Song-Bin
{"title":"Proteomic Analysis for the Identification of Proteins Related to Methotrexate Resistance","authors":"FENG Yuan-Xi , BAI Jing , ZHANG Chun-Yu , FU Song-Bin","doi":"10.1016/S0379-4172(06)60065-6","DOIUrl":"10.1016/S0379-4172(06)60065-6","url":null,"abstract":"<div><p>Methotrexate(MTX) is one of the most important and frequently used drugs in cancer therapy, but the efficacy of this drug is often compromised by the development of resistance in cancer cells. To seek and identify differentially expressed proteins related to MTX resistance and provide clues for the mechanism of MTX resistance, proteins from cell line MTX300 (resistant to 300 μmol/L MTX) and its control cell line 3T3R500 were separated by two-dimensional electrophoresis (2-DE). The colloidal Coomassie brilliant blue-stained 2-DE gels were subjected to image analysis, which revealed several spots with high levels of differential expression between MTX300 and 3T3R500. The protein spot with highest differential expression was submitted for tryptic peptide mass fingerprinting(PMF) for identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MS analysis and database searches revealed it to be dihydrofolate reductase (DHFR), which was subsequently confirmed by Western blot. The result suggested that DHFR might play an important role in the MTX resistance.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 5","pages":"Pages 391-396"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60065-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26041566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}