Acta Genetica Sinica最新文献_第8页

Analysis of Microsatellites in Citrus Unigenes 柑橘属植物微卫星分析

Acta Genetica Sinica Pub Date : 2006-04-01 DOI: 10.1016/S0379-4172(06)60060-7

JIANG Dong, ZHONG Guang-Yan, HONG Qi-Bing

{"title":"Analysis of Microsatellites in Citrus Unigenes","authors":"JIANG Dong, ZHONG Guang-Yan, HONG Qi-Bing","doi":"10.1016/S0379-4172(06)60060-7","DOIUrl":"10.1016/S0379-4172(06)60060-7","url":null,"abstract":"<div><p>Simple sequence repeats (SSRs) were investigated in the unigene sequences from expressed sequence tags (EST) of sweet orange (<em>Citrus sinensis osbeck</em>), trifoliate orange (<em>Poncirus trifoliata Raf.</em>) and other citrus species and cultivars. A total of 37 802 citrus unigene sequences corresponding to 23.29 Mb were searched, resulting in the identification of 8 218 SSRs. Among them there were 4 913 (59.8%) mono-, 1 419 (17.3%) di-, 1 709 (20.8%) tri-, 114 (1.39%) tetra-, 23 (0.28%) penta- and 40 (0.49%) hexa-nucleotide SSRs. The estimated frequency of SSRs was approximately 1/2.8 kb, which could be extrapolated to 1 SSR-containing unigene in 4.6 unigenes. The maximum length of the SSR ranged from 40 to 105 bp depending on the repeating numbers of the motif in the SSR. The overall average length of SSRs was 20.9 bp. The frequencies of different SSR types (di-, tri-, tetra-, and penta-nucleotide repeats) were very similar between sweet orange and trifoliate orange. The mononucelotide repeats appeared to be the most abundant SSRs within sweet orange and trifoliate orange, followed by trimeric repeats. The adenine rich repeats such as A/T, AG, AT, AAG, AAAT, AAAG, AAAT, AAAAG, AAAAT etc. were predominant in each type of SSRs (mono-, di-, tri-, tetra-, and penta-), whereas the C/G, CG, CCG repeats were less abundant. Twenty-five primer pairs flanking EST-SSR loci were designed to detect the possible polymorphism of six citrus cultivars including sweet orange and trifoliate orange. The PCR result with all these 25 primer pairs revealed the existence of polymorphism within six citrus cultivars confirming that citrus EST database could be efficiently exploited for the development of gene-derived SSR markers.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 4","pages":"Pages 345-353"},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60060-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25984652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Development of Transformation System of Rice Based on Binary Bacterial Artificial Chromosome (BIBAC) Vector 基于二元细菌人工染色体(BIBAC)载体的水稻转化体系的建立

Acta Genetica Sinica Pub Date : 2006-03-01 DOI: 10.1016/S0379-4172(06)60050-4

Rui-Feng HE, Yuan-Yuan WANG, Bo DU, Ming TANG, Ai-Qing YOU, Li-Li ZHU, Guang-Cun HE

{"title":"Development of Transformation System of Rice Based on Binary Bacterial Artificial Chromosome (BIBAC) Vector","authors":"Rui-Feng HE, Yuan-Yuan WANG, Bo DU, Ming TANG, Ai-Qing YOU, Li-Li ZHU, Guang-Cun HE","doi":"10.1016/S0379-4172(06)60050-4","DOIUrl":"10.1016/S0379-4172(06)60050-4","url":null,"abstract":"<div><h3>Abstract</h3><p>An <em>Agrobacterium</em>-mediated transformation protocol using binary bacterial artificial chromosome (BIBAC) vector system in rice (<em>Oryza sativa</em> L.) was developed. Calli derived from mature embryos of <em>japonica</em> rice cv. H1493 were used as target tissues. Various aspects in transformation and regeneration processes including callus induction and culture, <em>Agrobacterium</em> concentration and duration of co-cultivation, bacterial elimination and transformant selection were examined in order to improve the transformation efficiency. An optimized transformation conditions was established including: using an <em>Agrobacterium</em> strain, LBA4404(HP4404), which carries a super-virulent helper plasmid pCH32, for the infection; a modified N6 medium system for callus induction and culture; pH 5.6 for media in pre-cultivation and co-cultivation; <em>Agrobacterium</em> concentration at OD<sub>600</sub> = 1.0 for 3 days co-cultivation and 7 days for a resting period of the infected calli. Based on PCR and Southern blot analysis, it was demonstrated that insert DNA and marker genes carried by BIBAC2 were integrated into the rice genome.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 3","pages":"Pages 269-276"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60050-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25920551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Identify Female Carriers and De Novo Mutations in Deletional Duchenne/Becker Muscular Dystrophy Families 在缺失的杜氏/贝克尔肌营养不良家族中识别女性携带者和新生突变

Acta Genetica Sinica Pub Date : 2006-03-01 DOI: 10.1016/S0379-4172(06)60041-3

Hai-Yan ZHU, Ling-Qian WU, De-Sheng LIANG, Qian PAN, Jia-Hui XIA

{"title":"Identify Female Carriers and De Novo Mutations in Deletional Duchenne/Becker Muscular Dystrophy Families","authors":"Hai-Yan ZHU, Ling-Qian WU, De-Sheng LIANG, Qian PAN, Jia-Hui XIA","doi":"10.1016/S0379-4172(06)60041-3","DOIUrl":"10.1016/S0379-4172(06)60041-3","url":null,"abstract":"<div><h3>Abstract</h3><p>By using multiple polymerase reaction (mPCR) and haploid analysis of 11 short tandem repeats (STRs) in dystrophin gene locus to identify female carriers in deletional DMD/BMD (Duchenne/Becker Muscular Dystrophy) families, valuable information can be gathered for prenatal diagnosis. In this article, de novo mutations were detected in two out of the four patients, and one of the four female members was identified as an obligate <em>DMD</em> gene carrier based on the haplotype analysis. Multiple PCR and STRs haploid linkage analysis are rapid, accurate, objective methods to identify female member status, and well suited for routine use in clinical laboratories engaged in <em>DMD/BMD</em> research for counseling, gene diagnosis and prenatal diagnosis. During mPCR analysis, the amplicon of exon 45 showed different electrophoresis mobility in different kinds of gels. Polyacrylamide Gels Electrophoresis (PAGE) was accurate and rapid for analyzing the products of mPCR, but the mobility of different amplicons need to be considered in data analysis.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 3","pages":"Pages 206-212"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60041-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25918440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SSR Mapping of Brown Planthopper Resistance Gene Bph9 in Kaharamana, an Indica Rice (Oryza sativa L.) 籼稻抗褐飞虱基因Bph9的SSR定位

Acta Genetica Sinica Pub Date : 2006-03-01 DOI: 10.1016/S0379-4172(06)60049-8

Chang-Chao SU , Hu-Qu ZHAI , Chun-Ming WANG , Li-Hong SUN , Jian-Min WAN

{"title":"SSR Mapping of Brown Planthopper Resistance Gene Bph9 in Kaharamana, an Indica Rice (Oryza sativa L.)","authors":"Chang-Chao SU , Hu-Qu ZHAI , Chun-Ming WANG , Li-Hong SUN , Jian-Min WAN","doi":"10.1016/S0379-4172(06)60049-8","DOIUrl":"10.1016/S0379-4172(06)60049-8","url":null,"abstract":"<div><p>Abstract</p><p>The brown planthopper (BPH) is one of the most serious insects pests of rice, and the host resistance has been recognized as one of the most economic and effective measures for BPH management. In this study, we conducted a molecular-based genetic analysis of Bph9 in Kaharamana, a Sri Lanka rice variety resistant to BPH insects of East and Southeast Asia. An F<sub>2</sub> segregating population composed of 180 plants was constructed from the cross between Kaharamana and 02428, and each F<sub>2</sub> plant was self-crossed to obtain F<sub>2:3</sub> family. The bulked seedling test method was used to evaluate the resistance of F<sub>2:3</sub> families, and the genotype of each F<sub>2</sub> plant was inferred from the phenotype of corresponding F<sub>2:3</sub> family. Linkage analysis indicated that the resistant gene Bph9 in Kaharamana was located between SSR markers RM463 and RM5341 on chromosome 12 with linkage distances of 6.8 cM and 9.7 cM, respectively. The time- and money-saving SSR markers would be helpful in the application of Bph9 in breeding program via marker-assisted selection.</p></div>","PeriodicalId":100017,"journal":{"name":"Acta Genetica Sinica","volume":"33 3","pages":"Pages 262-268"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0379-4172(06)60049-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25920550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 51

Gene Trapping Techniques and Current Progress 基因捕获技术及其最新进展

Acta Genetica Sinica Pub Date : 2006-03-01 DOI: 10.1016/S0379-4172(06)60039-5

Yuan-Yuan LI, Jing-Pu ZHANG

引用次数: 2

Frontiers of Plant Biology, 2006 植物学报，2006

Acta Genetica Sinica Pub Date : 2006-03-01 DOI: 10.1016/S0379-4172(06)60044-9

引用次数: 0

11th International Association for Plant Tissue Culture & Biotechnology 第十一届国际植物组织培养与生物技术协会

Acta Genetica Sinica Pub Date : 2006-03-01 DOI: 10.1016/S0379-4172(06)60052-8

引用次数: 0

Isolation and Characterization of Defense Response Genes Involved in Neck Blast Resistance of Rice 水稻抗颈瘟病防御反应基因的分离与鉴定