Cell Research最新文献_第9页

Pyroptosis: molecular mechanisms and roles in disease 焦亡：分子机制及其在疾病中的作用。

IF 25.9 1区生物学

Cell Research Pub Date : 2025-04-03 DOI: 10.1038/s41422-025-01107-6

Petr Broz

{"title":"Pyroptosis: molecular mechanisms and roles in disease","authors":"Petr Broz","doi":"10.1038/s41422-025-01107-6","DOIUrl":"10.1038/s41422-025-01107-6","url":null,"abstract":"Pyroptosis is a type of programmed necrosis triggered by the detection of pathogens or endogenous danger signals in the cytosol. Pyroptotic cells exhibit a swollen, enlarged morphology and ultimately undergo lysis, releasing their cytosolic contents — such as proteins, metabolites, and nucleic acids — into the extracellular space. These molecules can function as danger-associated molecular patterns (DAMPs), triggering inflammation when detected by neighboring cells. Mechanistically, pyroptosis is initiated by members of the gasdermin protein family, which were identified a decade ago as pore-forming executors of cell death. Mammalian gasdermins consist of a cytotoxic N-terminal domain, a flexible linker, and a C-terminal regulatory domain that binds to and inhibits the N-terminus. Proteolytic cleavage within the linker releases the N-terminal domain, enabling it to target various cellular membranes, including nuclear, mitochondrial, and plasma membranes, where it forms large transmembrane pores. Gasdermin pores in the plasma membrane disrupt the electrochemical gradient, leading to water influx and cell swelling. Their formation also activates the membrane protein ninjurin-1 (NINJ1), which oligomerizes to drive complete plasma membrane rupture and the release of large DAMPs. Since their discovery as pore-forming proteins, gasdermins have been linked to pyroptosis not only in host defense but also in various pathological conditions. This review explores the history of pyroptosis, recent insights into gasdermin activation, the cellular consequences of pore formation, and the physiological roles of pyroptosis.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"35 5","pages":"334-344"},"PeriodicalIF":25.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of chromatin remodeling by the human Snf2-type ATPase SNF2H 人类snf2型atp酶SNF2H的染色质重塑机制。

IF 25.9 1区生物学

Cell Research Pub Date : 2025-04-03 DOI: 10.1038/s41422-025-01103-w

Deepshikha Malik, Ashish Deshmukh, Silvija Bilokapic, Mario Halic

引用次数: 0

Signal-induced NLRP3 phase separation initiates inflammasome activation 信号诱导的NLRP3相分离启动炎性小体激活

IF 25.9 1区生物学

Cell Research Pub Date : 2025-04-01 DOI: 10.1038/s41422-025-01096-6

Gonglu Zou, Yuluan Tang, Jie Yang, Shuo Fu, Yuheng Li, Xuanyao Ren, Nanhai Zhou, Wenlong Zhao, Juyi Gao, Ziran Ruan, Zhengfan Jiang

{"title":"Signal-induced NLRP3 phase separation initiates inflammasome activation","authors":"Gonglu Zou, Yuluan Tang, Jie Yang, Shuo Fu, Yuheng Li, Xuanyao Ren, Nanhai Zhou, Wenlong Zhao, Juyi Gao, Ziran Ruan, Zhengfan Jiang","doi":"10.1038/s41422-025-01096-6","DOIUrl":"10.1038/s41422-025-01096-6","url":null,"abstract":"NLRP3 inflammasome is activated by diverse stimuli including infections, intracellular and environmental irritants. How NLRP3 senses these unrelated stimuli and what activates NLRP3 remain unknown. Here we report that signal-dependent NLRP3 phase separation initiated its activation, in which the palmitoyltransferase ZDHHC7-mediated tonic NLRP3 palmitoylation and an IDR region in the FISNA domain of NLRP3 play important roles. Moreover, three conserved hydrophobic residues in the IDR critically mediate multivalent weak interactions. NLRP3-activating stimuli including K+ efflux and NLRP3-interacting molecules imiquimod, palmitate, and cardiolipin all cause NLRP3 conformational change and induce its phase separation and activation in cells and/or in vitro. Surprisingly, amphiphilic molecules like di-alcohols used to inhibit biomolecular phase separation and chemotherapeutic drugs doxorubicin and paclitaxel activate NLRP3 independently of ZDHHC7 by directly inducing NLRP3 phase separation. Mechanistically, amphiphilic molecules decrease the solubility of both palmitoylated and non-palmitoylated NLRP3 to directly induce its phase separation and activation while NLRP3 palmitoylation reduces its solubility to some extent without activation. Therefore, ZDHHC7-mediated NLRP3 palmitoylation in resting cells licenses its activation by lowering the threshold for NLRP3 phase separation in response to any of the diverse stimuli whereas NLRP3 solubility-reducing molecules like di-alcohols and chemotherapeutic drugs activate NLRP3 directly. The signal-induced NLRP3 phase separation likely provides the simplest and most direct mechanistic basis for NLRP3 activation.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"35 6","pages":"437-452"},"PeriodicalIF":25.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41422-025-01096-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A phytoalexin dismantles bacterial type III injectisome 一种植物抗菌素可以分解细菌III型注射体

IF 25.9 1区生物学

Cell Research Pub Date : 2025-03-31 DOI: 10.1038/s41422-025-01111-w

Manman Zhang, Cheng Li, Zheng Qing Fu

引用次数: 0

Author Correction: Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes 作者更正：通过植入诱导的人类功能性肝细胞的生物人工肝装置提高猪急性肝衰竭的存活率。

IF 25.9 1区生物学

Cell Research Pub Date : 2025-03-27 DOI: 10.1038/s41422-025-01108-5

Xiao-Lei Shi, Yimeng Gao, Yupeng Yan, Hucheng Ma, Lulu Sun, Pengyu Huang, Xuan Ni, Ludi Zhang, Xin Zhao, Haozhen Ren, Dan Hu, Yan Zhou, Feng Tian, Yuan Ji, Xin Cheng, Guoyu Pan, Yi-Tao Ding, Lijian Hui

引用次数: 0

Grow AI virtual cells: three data pillars and closed-loop learning 培育人工智能虚拟细胞：三大数据支柱和闭环学习。

IF 25.9 1区生物学

Cell Research Pub Date : 2025-03-25 DOI: 10.1038/s41422-025-01101-y

Liujia Qian, Zhen Dong, Tiannan Guo

引用次数: 0

Mechano-pharmacology of T cell receptor specificity T细胞受体特异性的机械药理学。

IF 25.9 1区生物学

Cell Research Pub Date : 2025-03-25 DOI: 10.1038/s41422-025-01105-8

Panyu Fei, Michael L. Dustin

引用次数: 0

Broad H3K4me3 domains orchestrate temporal control of gene expression 广泛的H3K4me3结构域协调基因表达的时间控制。