Cell Research最新文献_第10页

Zinc accumulation-induced integrated stress response triggers β-cell identity loss 锌积累诱导的综合应激反应引发β细胞身份丧失

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-28 DOI: 10.1038/s41422-026-01222-y

Qing Ma, Wenjun Xu, Xuan Wang, Haoyu Nie, Yukun Gao, Rui Hu, Zhihao Yang, Xushu Wang, Ta Na, Xiangyi Chen, Zhaoyue Wang, Minglu Xu, Li Shao, Meng Guo, Yanfang Liu, Rongrong Le, Shaorong Gao, Weida Li

{"title":"Zinc accumulation-induced integrated stress response triggers β-cell identity loss","authors":"Qing Ma, Wenjun Xu, Xuan Wang, Haoyu Nie, Yukun Gao, Rui Hu, Zhihao Yang, Xushu Wang, Ta Na, Xiangyi Chen, Zhaoyue Wang, Minglu Xu, Li Shao, Meng Guo, Yanfang Liu, Rongrong Le, Shaorong Gao, Weida Li","doi":"10.1038/s41422-026-01222-y","DOIUrl":"10.1038/s41422-026-01222-y","url":null,"abstract":"Pancreatic β-cell identity loss is increasingly recognized as a critical pathogenic contributor to β-cell failure in type 2 diabetes (T2D), but the specific mechanism remains to be characterized. In this study, we demonstrate that zinc accumulation contributes to β-cell identity loss during diabetes progression in both human and mouse islets. Using a model of human embryonic stem cell-derived islets (SC-islets), we reveal that accumulated zinc triggers the integrated stress response (ISR), with elevated ATF4 expression in SC-β cells. This, in turn, initiates expression of the α cell-specific transcription factor ARX, resulting in the conversion of β cells to α cells, thus forming a zinc-ATF4-ARX regulatory axis. Like primary β cells, SC-β cells also undergo identity loss after transplantation into diabetic animals, which can be prevented by an ISR inhibitor, resulting in improved glycemic control. Furthermore, both genetic depletion and chemical inhibition of zinc accumulation effectively safeguard SC-β cells from identity loss and enhance their efficacy in diabetic animals. Our study thus reveals a pathogenic mechanism in which zinc accumulation induces β-cell identity loss through lineage-tracing approaches and proposes a protective strategy to counteract this process.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"36 5","pages":"359-376"},"PeriodicalIF":25.9,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41422-026-01222-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146057207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The AHCY–adenosine complex rewires mRNA methylation to enhance fatty acid biosynthesis and tumorigenesis ahcy -腺苷复合体重新连接mRNA甲基化以增强脂肪酸的生物合成和肿瘤发生

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-19 DOI: 10.1038/s41422-025-01213-5

Kun Liao, Fen Cao, Chen Wei, Zheng-Yu Qian, Hong-Rong Hu, Wen-Feng Pan, Zi-Qing Feng, Sen-mao Lian, Zi-Xuan Xiao, Hui Sheng, Hai-Yu Mo, Yi-Xuan Zhao, Qi-Nian Wu, Zhao-Lei Zeng, Bo Li, Rui-Hua Xu, Huai-Qiang Ju

{"title":"The AHCY–adenosine complex rewires mRNA methylation to enhance fatty acid biosynthesis and tumorigenesis","authors":"Kun Liao, Fen Cao, Chen Wei, Zheng-Yu Qian, Hong-Rong Hu, Wen-Feng Pan, Zi-Qing Feng, Sen-mao Lian, Zi-Xuan Xiao, Hui Sheng, Hai-Yu Mo, Yi-Xuan Zhao, Qi-Nian Wu, Zhao-Lei Zeng, Bo Li, Rui-Hua Xu, Huai-Qiang Ju","doi":"10.1038/s41422-025-01213-5","DOIUrl":"10.1038/s41422-025-01213-5","url":null,"abstract":"Methionine metabolism generates the substrate S-adenosylmethionine (SAM), which regulates epigenetic modifications crucial for various cellular processes, particularly tumorigenesis. However, whether methionine metabolism involves epigenetic mechanisms independent of SAM and what roles such mechanisms play in tumorigenesis remain unclear. We show here that the adenosylhomocysteinase (AHCY)–adenosine complex increases mRNA m6A levels in a non-global manner, promoting fatty acid synthesis and tumorigenesis. Adenosine increases mRNA m6A levels by binding to the methionine metabolism enzyme AHCY to form a complex, rather than depending on adenosine receptors. The AHCY–adenosine complex facilitates AHCY dimerization, with adenosine being crucial for dimer stability. AHCY dimers hinder the binding of fat mass and obesity-associated protein (FTO) at the Q86 site to RNA containing the VWDRACH motif, increasing m6A levels and upregulating lipogenesis genes, especially ACACA and SCD1, thus leading to reprogramming of lipid metabolism. Conversely, AHCY mutants that have lost dimerization or FTO-binding ability but retain hydrolase activity suppress lipogenesis and tumor growth without significantly affecting methionine catabolism mediated by AHCY. Loss of AHCY in mice and disruption of AHCY dimerization in tumor cells and patient-derived xenograft models restricted tumor growth. Our findings demonstrate a key SAM-independent link between methionine metabolism and mRNA m6A modification that affects demethylase substrate specificity. This novel link between the methionine cycle and lipid metabolism suggests new strategies for anticancer therapy.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"36 2","pages":"152-172"},"PeriodicalIF":25.9,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41422-025-01213-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145993482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting immunotherapy by restoring cryptic APC function 通过恢复隐蔽性APC功能促进免疫治疗。

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-16 DOI: 10.1038/s41422-026-01219-7

Wonhwa Cho

引用次数: 0

Efficient chemical reprogramming of human T cells to pluripotent stem cells 人类T细胞转化为多能干细胞的高效化学重编程。

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-16 DOI: 10.1038/s41422-025-01216-2

Yanglu Wang, Fangqi Peng, Ruoqi Cheng, Jingran Zeng, Weizhen Zeng, Jingping Mao, Xiaodi Fu, Guanxian Chen, Tianxing Liu, Zhihan Yang, Cheng Li, Bei Liu, Jingyang Guan, Lin Cheng, Hongkui Deng

引用次数: 0

Gut dysbiosis in oncology: a risk factor for immunoresistance 肿瘤学中的肠道生态失调：免疫抵抗的危险因素。

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-14 DOI: 10.1038/s41422-025-01212-6

Andrew Allan Almonte, Simon Thomas, Valerio Iebba, Guido Kroemer, Lisa Derosa, Laurence Zitvogel

{"title":"Gut dysbiosis in oncology: a risk factor for immunoresistance","authors":"Andrew Allan Almonte, Simon Thomas, Valerio Iebba, Guido Kroemer, Lisa Derosa, Laurence Zitvogel","doi":"10.1038/s41422-025-01212-6","DOIUrl":"10.1038/s41422-025-01212-6","url":null,"abstract":"The gut microbiome is recognized as a determinant of response to immune checkpoint inhibitor (ICI) therapies in cancer. However, the clinical translation of microbiome science has been hampered by inconsistent definitions of dysbiosis, inadequate biomarker frameworks, and limited mechanistic understanding. In this review, we synthesize the current state of knowledge on how gut microbial composition and function influence ICI efficacy, highlighting both correlative and causal evidence. We discuss computational approaches based on α-diversity or taxonomic abundance and argue for more functionally and clinically informative models, such as the topological score (TOPOSCORE) and other dysbiosis indices derived from machine learning. Using retrospective analyses of metagenomic datasets from thousands of patients and healthy controls, we examine microbial patterns that distinguish responders from non-responders. We also explore how dysbiosis perturbs immunoregulatory pathways, including bile acid metabolism, gut permeability, and mucosal immunomodulation. Finally, we assess emerging therapeutic strategies aimed at correcting microbiome dysfunction — including dietary modification, bacterial consortia, and fecal microbiota transplantation — and describe how they are being deployed in multiple clinical trials. We conclude with a brief discussion of the ONCOBIOME initiative, which works with international partners to incorporate microbiome science into oncology workflows. By refining our understanding of gut–immune interactions and translating it into action, microbiome-informed oncology may unlock new therapeutic potential for patients previously resistant to immunotherapy.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"36 2","pages":"103-120"},"PeriodicalIF":25.9,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41422-025-01212-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145968492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactate-activated GPR81/FARP1 signaling drives insulin-independent glucose uptake and metabolic control 乳酸激活的GPR81/FARP1信号驱动胰岛素不依赖的葡萄糖摄取和代谢控制

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-13 DOI: 10.1038/s41422-025-01207-3

Yaxin Niu, Shengmin Hu, Yanfeng Zhang, Jinbao Yang, Jiarui Zhang, Ruiping He, Li Chen, Lin Xu, Hongfang Zhao, Bing Gan, Ruobing Ren, Ruth J. F. Loos, Haobin Ye, Xingrong Du, Tongjin Zhao, Peng Li, Antonio Vidal-Puig, Linzhang Huang

{"title":"Lactate-activated GPR81/FARP1 signaling drives insulin-independent glucose uptake and metabolic control","authors":"Yaxin Niu, Shengmin Hu, Yanfeng Zhang, Jinbao Yang, Jiarui Zhang, Ruiping He, Li Chen, Lin Xu, Hongfang Zhao, Bing Gan, Ruobing Ren, Ruth J. F. Loos, Haobin Ye, Xingrong Du, Tongjin Zhao, Peng Li, Antonio Vidal-Puig, Linzhang Huang","doi":"10.1038/s41422-025-01207-3","DOIUrl":"10.1038/s41422-025-01207-3","url":null,"abstract":"Insulin-stimulated glucose uptake is central to global carbohydrate metabolism, yet metabolites that enhance glucose uptake independently of insulin remain undefined. Here, we identify L-lactate as an insulin-independent regulator of glucose uptake that mitigates hyperglycemia. Loss of LDHA in muscle reduces lactate production, impairing glucose homeostasis in mice. By contrast, lactate administration or genetic upregulation of lactate production improves glucose control. Knockout of the lactate receptor GPR81 in skeletal muscle worsens glucose tolerance, whereas its ectopic expression or pharmacological activation enhances carbohydrate metabolism. Mechanistically, GPR81 recruits FARP1 to activate RAC1, promoting GLUT4 translocation independently of insulin signaling. Notably, the expression of LDHA, GPR81, and FARP1 is upregulated after exercise, and GPR81 variants are highly correlated with fasting insulin levels in humans, underscoring the synergy of the GPR81-FARP1-GLUT4 axis with insulin in glucose regulation. Our findings suggest that targeting GPR81 represents a potential insulin-independent strategy for the treatment of hyperglycemia.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"36 2","pages":"137-151"},"PeriodicalIF":25.9,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145956283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria target the plasma membrane to cause mitoxyperiosis 线粒体以质膜为靶点，引起有丝分裂。

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-13 DOI: 10.1038/s41422-025-01215-3

Jaiya Randhawa, Luke A. J. O’Neill

引用次数: 0

Gz and β-arrestin 1 signaling in the μ-opioid receptor Gz和β-阻滞蛋白1信号在μ-阿片受体中的作用。

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-09 DOI: 10.1038/s41422-025-01214-4

Zhi Cheng, Bryan L. Roth

引用次数: 0

Transient mechanical activation of the Piezo1 channel facilitates ex vivo expansion of hematopoietic stem cells Piezo1通道的瞬时机械激活促进了造血干细胞的体外扩增

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-09 DOI: 10.1038/s41422-025-01209-1

Qiwei Wang, Xin Zeng, Haoxiang Yang, Huan Lu, Lingli Jiang, Lizhen Xu, Jinxin Li, Jingyi Li, Yingli Han, Xiaoyan Wu, Yuanhong Zhou, Xiaolan Chen, Yanmin Zhao, Jimin Shi, Yi Luo, Fang Ni, Jie Sun, Qian Zhao, Fan Yang, Peng Xia, Hongyuan Jiang, He Huang, Pengxu Qian

{"title":"Transient mechanical activation of the Piezo1 channel facilitates ex vivo expansion of hematopoietic stem cells","authors":"Qiwei Wang, Xin Zeng, Haoxiang Yang, Huan Lu, Lingli Jiang, Lizhen Xu, Jinxin Li, Jingyi Li, Yingli Han, Xiaoyan Wu, Yuanhong Zhou, Xiaolan Chen, Yanmin Zhao, Jimin Shi, Yi Luo, Fang Ni, Jie Sun, Qian Zhao, Fan Yang, Peng Xia, Hongyuan Jiang, He Huang, Pengxu Qian","doi":"10.1038/s41422-025-01209-1","DOIUrl":"10.1038/s41422-025-01209-1","url":null,"abstract":"Achieving long-term ex vivo expansion of functional hematopoietic stem cells (HSCs) is essential for advancing HSC-based clinical therapies. Although mechanosensitive ion channels are known to play key roles in the hematopoietic system, their involvement in HSC expansion remains unclear. Here, we show that Piezo1 is highly expressed in HSCs. Both genetic deletion and prolonged chemical activation of Piezo1 impair cultured HSC function, indicating that transient mechanical activation of Piezo1 is required for maintenance of HSCs in culture. To achieve this, we screened various microspheres and found that PS500 (500-nm polystyrene microspheres) significantly enhanced ex vivo expansion of mouse bone marrow HSCs with long-term repopulating capacity. PS500 also expanded human umbilical cord blood HSCs capable of engraftment in immunodeficient mice. Mechanistically, PS500 activates Piezo1, triggering Ca2+-dependent expression of proliferative cytokines and subsequent STAT3 activation, which support HSC self-renewal and proliferation. Together, these findings show that PS500 enables transient Piezo1 activation and efficient, non-toxic expansion of functional HSCs, offering a promising approach for the generation of transplantable HSCs for clinical use.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"36 4","pages":"272-285"},"PeriodicalIF":25.9,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41422-025-01209-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

R-loops orchestrate RNAPII transcriptional reprogramming for the maternal-to-zygotic transition r -环协调RNAPII转录重编程，以实现母体到合子的转变

IF 25.9 1区生物学

Cell Research Pub Date : 2026-01-09 DOI: 10.1038/s41422-025-01208-2

Yaoyi Li, Qing Li, Xinxiu Wang, Chao Di, Yingliang Sheng, Ying Ma, Junzhi Liao, Qingqing Cai, Sainan Huang, Jiayu Chen, Guangming Wu, Lingling Zhang, Guangjin Pan, Shaorong Gao, Hongjie Yao

{"title":"R-loops orchestrate RNAPII transcriptional reprogramming for the maternal-to-zygotic transition","authors":"Yaoyi Li, Qing Li, Xinxiu Wang, Chao Di, Yingliang Sheng, Ying Ma, Junzhi Liao, Qingqing Cai, Sainan Huang, Jiayu Chen, Guangming Wu, Lingling Zhang, Guangjin Pan, Shaorong Gao, Hongjie Yao","doi":"10.1038/s41422-025-01208-2","DOIUrl":"10.1038/s41422-025-01208-2","url":null,"abstract":"R-loops are pervasive genomic structures that link epigenetic modification and transcriptional regulation. However, the functional roles and regulatory mechanisms of R-loops during preimplantation development in mammals remain unexplored. Here, we reveal that the reprogramming of R-loops across developmental stages depends on CG density, with CG-poor R-loops more stage specific and strongly associated with early embryonic development. Loss of CG-poor R-loops causes severe defects in the maternal-to-zygotic transition (MZT) and preimplantation embryo development. This abnormal maintenance of CG-poor R-loops promotes premature activation of major zygotic genome activation (ZGA) genes. CG-poor R-loops inhibit DDX21 helicase activity on the 7SK/HEXIM1 snRNP complex, restricting CDK9 release and subsequent phosphorylation of Ser2 at the C-terminal domain of RNA polymerase II (RNAPII S2p) — the biochemical hallmark of pause release — thus enforcing RNAPII accumulation at major ZGA gene promoters to ensure productive transcription. These findings establish R-loops as direct modulators of RNAPII pause release, promoting the temporal fidelity of gene expression during the MZT.","PeriodicalId":9926,"journal":{"name":"Cell Research","volume":"36 3","pages":"181-196"},"PeriodicalIF":25.9,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0