Cell RegenerationPub Date : 2022-06-02DOI: 10.1186/s13619-022-00121-y
Xing Qin, Juanjuan Fei, Yu Duan, Asli F Ceylan, Fuyang Zhang, Jun Ren
{"title":"Beclin1 haploinsufficiency compromises mesenchymal stem cell-offered cardioprotection against myocardial infarction.","authors":"Xing Qin, Juanjuan Fei, Yu Duan, Asli F Ceylan, Fuyang Zhang, Jun Ren","doi":"10.1186/s13619-022-00121-y","DOIUrl":"10.1186/s13619-022-00121-y","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs)-based therapy has displayed some promises in ischemia heart diseases although its efficacy may be affected by changes in surrounding environments. This study evaluated the role of autophagy insufficiency using Beclin1 haploinsufficiency (BECN<sup>+/-</sup>) on intra-myocardial MSC transplantation-evoked effect against myocardial infarction. Donor MSCs from C57BL/6 mice were labelled with cell-tracker CM Dil and were delivered into LV free wall adjacent to infarct region in wild-type (WT) and BECN<sup>+/-</sup> recipient mice following ligation of left main coronary artery (MI-MSCs). Ten days following MI, myocardial function was assessed using echocardiography. Cardiomyocyte contractility and intracellular Ca<sup>2+</sup> were monitored using cardiomyocytes from the area-at-risk adjacent to infarct. CM-Dil labeled cells were tracked in MSCs recipient mice using fluorescence microscopy. Lectin, Masson trichrome staining and Western blot analysis were employed to determine cardiomyocyte area, scar fibrosis, apoptosis and inflammation. MI insult triggered scar fibrosis, LV chamber dilation, decreased fractional shortening, ejection fraction, cardiomyocyte shortening, maximal velocity of shortening and relengthening as well as prolonged relengthening, which were abrogated or attenuated by MSCs therapy in WT but not BECN<sup>+/-</sup> mice. MI decreased intracellular Ca<sup>2+</sup> rise and decay in response to electrical stimuli without affecting resting intracellular Ca<sup>2+</sup>, which were reconciled by MSCs in WT but not BECN<sup>+/-</sup> mice. MSCs further attenuated MI-induced mitochondrial ultrastructural injury, apoptosis, inflammation and autophagy defects in peri-infarct area in WT but not BECN<sup>+/-</sup> mice. Collectively, our results suggested that autophagy insufficiency dampened in MSCs-elicited cardioprotection associated with dampened apoptosis and inflammation.</p>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":"21"},"PeriodicalIF":4.0,"publicationDate":"2022-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell RegenerationPub Date : 2022-05-05DOI: 10.1186/s13619-022-00118-7
Haonan Li, Xiaodan Wang, Yalong Wang, Mengxian Zhang, F. Hong, Hong Wang, Along Cui, Jianguo Zhao, W. Ji, Ye-Guang Chen
{"title":"Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium","authors":"Haonan Li, Xiaodan Wang, Yalong Wang, Mengxian Zhang, F. Hong, Hong Wang, Along Cui, Jianguo Zhao, W. Ji, Ye-Guang Chen","doi":"10.1186/s13619-022-00118-7","DOIUrl":"https://doi.org/10.1186/s13619-022-00118-7","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43438279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell RegenerationPub Date : 2022-05-05DOI: 10.1186/s13619-022-00120-z
Nevan Powers, Guo N. Huang
{"title":"Mending a broken heart with novel cardiogenic small molecules","authors":"Nevan Powers, Guo N. Huang","doi":"10.1186/s13619-022-00120-z","DOIUrl":"https://doi.org/10.1186/s13619-022-00120-z","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell RegenerationPub Date : 2022-05-02DOI: 10.1186/s13619-022-00116-9
Muchuan Wang, Ziquan Li, Sen Zhao, Zhifa Zheng, Yi‐peng Wang, G. Qiu, Zhihong Wu, N. Wu, T. Zhang, Siyi Cai
{"title":"The identification of PAX7 variants and a potential role of muscle development dysfunction in congenital scoliosis","authors":"Muchuan Wang, Ziquan Li, Sen Zhao, Zhifa Zheng, Yi‐peng Wang, G. Qiu, Zhihong Wu, N. Wu, T. Zhang, Siyi Cai","doi":"10.1186/s13619-022-00116-9","DOIUrl":"https://doi.org/10.1186/s13619-022-00116-9","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42974272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell RegenerationPub Date : 2022-05-01DOI: 10.1186/s13619-022-00113-y
Changmei Niu, Liyang Wang, Dongdong Ji, Mingjun Ren, Dongxu Ke, Qiang Fu, Kaile Zhang, Xi Yang
{"title":"Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study","authors":"Changmei Niu, Liyang Wang, Dongdong Ji, Mingjun Ren, Dongxu Ke, Qiang Fu, Kaile Zhang, Xi Yang","doi":"10.1186/s13619-022-00113-y","DOIUrl":"https://doi.org/10.1186/s13619-022-00113-y","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47841717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell RegenerationPub Date : 2022-04-20DOI: 10.1186/s13619-022-00108-9
D. Di Girolamo, S. Tajbakhsh
{"title":"Pathological features of tissues and cell populations during cancer cachexia","authors":"D. Di Girolamo, S. Tajbakhsh","doi":"10.1186/s13619-022-00108-9","DOIUrl":"https://doi.org/10.1186/s13619-022-00108-9","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}