Cell Regeneration最新文献_第6页

Molecular switch model for cardiomyocyte proliferation 心肌细胞增殖的分子开关模型

Cell Regeneration Pub Date : 2019-06-01 DOI: 10.1016/j.cr.2018.11.002

Satwat Hashmi, H.R. Ahmad

{"title":"Molecular switch model for cardiomyocyte proliferation","authors":"Satwat Hashmi, H.R. Ahmad","doi":"10.1016/j.cr.2018.11.002","DOIUrl":"10.1016/j.cr.2018.11.002","url":null,"abstract":"<div><p>This review deals with the human adult cardiomyocyte proliferation as a potential source for heart repair after injury. The mechanism to regain the proliferative capacity of adult cardiomyocytes is a challenge. However, recent studies are promising in showing that the ‘locked’ cell cycle of adult cardiomyocytes could be released through modulation of cell cycle checkpoints. In support of this are the signaling pathways of Notch, Hippo, Wnt, Akt and Jak/Stat that facilitate or inhibit the transition at cell cycle checkpoints. Cyclins and cyclin dependant kinases (CDKs) facilitate this transition which in turn is regulated by inhibitory action of pocket protein e.g. p21, p27 and p57. Transcription factors e.g. E2F, GATA4, TBx20 up regulate Cyclin A, A2, D, E, and CDK4 as promoters of cell cycle and Meis-1 and HIF-1 alpha down regulate cyclin D and E to inhibit the cell cycle. Paracrine factors like Neuregulin-1, IGF-1 and Oncostatin M and Extracellular Matrix proteins like Agrin have been involved in cardiomyocyte proliferation and dedifferentiation processes.</p><p>A molecular switch model is proposed that transforms the post mitotic cell into an actively dividing cell. This model shows how the cell cycle is regulated through on- and off switch mechanisms through interaction of transcription factors and signaling pathways with proteins of the cell cycle checkpoints. Signals triggered by injury may activate the right combination of the various pathways that can ‘switch on’ the proliferation signals leading to myocardial regeneration.</p></div>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"8 1","pages":"Pages 12-20"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cr.2018.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37075034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming IIa类组蛋白去乙酰化酶的核细胞质穿梭调节体细胞重编程

Cell Regeneration Pub Date : 2019-06-01 DOI: 10.1016/j.cr.2018.11.001

Zhiwei Luo , Xiaobing Qing , Christina Benda , Zhijian Huang , Meng Zhang , Yinghua Huang , Hui Zhang , Lulu Wang , Yiwei Lai , Carl Ward , Giacomo Volpe , Xiaofen Zhong , Baoming Qin , Qiang Zhuang , Miguel A. Esteban , Wenjuan Li

{"title":"Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming","authors":"Zhiwei Luo , Xiaobing Qing , Christina Benda , Zhijian Huang , Meng Zhang , Yinghua Huang , Hui Zhang , Lulu Wang , Yiwei Lai , Carl Ward , Giacomo Volpe , Xiaofen Zhong , Baoming Qin , Qiang Zhuang , Miguel A. Esteban , Wenjuan Li","doi":"10.1016/j.cr.2018.11.001","DOIUrl":"10.1016/j.cr.2018.11.001","url":null,"abstract":"<div><p>Class IIa histone deacetylases (HDACs) are a subfamily of HDACs with important functions in development and adult tissue homeostasis. As opposed to other HDACs, they lack catalytic function and bind transcription factors to recruit transcriptional co-regulators, mostly co-repressors such as nuclear receptor co-repressor (NCoR)/silencing mediator of retinoid and thyroid hormone receptor (SMRT). Class IIa HDACs enhance mouse somatic cell reprogramming to induced pluripotent stem cells (iPSCs) by repressing the function of the pro-mesenchymal transcription factor myocyte enhancer factor 2 (MEF2), which is upregulated during this process. Here, we describe, using HDAC4 and 7 as examples, that class IIa HDACs exhibit nuclear-cytoplasmic trafficking in reprogramming, being mostly cytoplasmic in donor fibroblasts and intermediate cells but translocating to the nucleus in iPSCs. Importantly, over-expressing a mutant form of HDAC4 or 7 that becomes trapped in the nucleus enhances the early phase of reprogramming but is deleterious afterwards. The latter effect is mediated through binding to the exogenous reprogramming factors at pluripotency loci, and the subsequent recruitment of NCoR/SMRT co-repressors. Thus, our findings uncover a context-dependent function of class IIa HDACs in reprogramming and further reinforce the idea that recruitment of co-repressors by the exogenous factors is a major obstacle for reactivating the pluripotency network in this process.</p></div>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"8 1","pages":"Pages 21-29"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cr.2018.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37075035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Approaches for generation of anti-leukemia specific T cells 抗白血病特异性T细胞的生成方法

Cell Regeneration Pub Date : 2018-12-01 DOI: 10.1016/j.cr.2018.09.002

Zhenyi Jin , Ling Xu , Yangqiu Li

引用次数: 5

Research advances in erythrocyte regeneration sources and methods in vitro 红细胞体外再生来源及方法的研究进展

Cell Regeneration Pub Date : 2018-12-01 DOI: 10.1016/j.cr.2018.10.001

Shuming Sun , Yuanliang Peng , Jing Liu

{"title":"Research advances in erythrocyte regeneration sources and methods in vitro","authors":"Shuming Sun , Yuanliang Peng , Jing Liu","doi":"10.1016/j.cr.2018.10.001","DOIUrl":"10.1016/j.cr.2018.10.001","url":null,"abstract":"<div><p>Erythrocytes (red blood cells, RBCs) facilitate gas exchange in the lungs and transport oxygen to the tissues. The human body must maintain erythrocyte regeneration to support metabolically active cells and tissues. In many hematological diseases, erythrocyte regeneration is impaired. Researchers have studied erythrocyte regeneration for many years both <em>in vivo</em> and <em>in vitro</em>. In this review, we summarize the sources and main culture methods for generating mature and functional red blood cells <em>in vitro</em>. Hematopoietic stem cells (HSCs), embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are classic sources for erythrocyte regeneration. In addition, alternative sources such as immortalized adult human erythroid cell lines and transformed fibroblasts have also been generated and have produced functional red blood cells. The culture systems for erythrocytes differ among laboratories. Researchers hope that improvements in culture techniques may contribute to improved RBC outcomes for blood transfusions, drug delivery and the treatment of hematological diseases.</p></div>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"7 2","pages":"Pages 45-49"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cr.2018.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36888213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Illumination of neural development by in vivo clonal analysis 体内克隆分析对神经发育的启示

Cell Regeneration Pub Date : 2018-12-01 DOI: 10.1016/j.cr.2018.09.001

Mingrui Xu , Jingjing Wang , Xize Guo , Tingting Li , Xia Kuang , Qing-Feng Wu

引用次数: 4

Long non-coding RNAs in hematopoietic regulation 长链非编码rna在造血调节中的作用

Cell Regeneration Pub Date : 2018-12-01 DOI: 10.1016/j.cr.2018.08.001

Weiqian Li , Yue Ren , Yanmin Si , Fang Wang , Jia Yu

引用次数: 16

Mouse embryonic stem cells resist c-Jun induced differentiation when in suspension 小鼠胚胎干细胞在悬浮状态下抵抗c-Jun诱导的分化

Cell Regeneration Pub Date : 2018-09-01 DOI: 10.1016/j.cr.2018.05.002

Bo Wang , Dongwei Li , Jiekai Chen , Jing Liu , Duanqing Pei

{"title":"Mouse embryonic stem cells resist c-Jun induced differentiation when in suspension","authors":"Bo Wang , Dongwei Li , Jiekai Chen , Jing Liu , Duanqing Pei","doi":"10.1016/j.cr.2018.05.002","DOIUrl":"10.1016/j.cr.2018.05.002","url":null,"abstract":"<div><p>The oncogene <em>c-Jun</em> plays a key role in development and cancer. Yet, its role in cell fate decision remains poorly understood at the molecular level. Here we report that c-Jun confers different fate decisions upon mouse embryonic stem cells (mESCs) in adhesion vs suspension culture. We developed a Tet-on system for temporal induction of c-Jun expression by Doxycycline treatment in mESCs. We show that mESCs carrying the inducible c-Jun TetOn remain pluripotent and grow slowly in suspension when c-Jun expression is induced, whilst when the cells adhere they undergo differentiation and show normal proliferative potential upon c-Jun induction. Our data indicates that c-Jun pushes mESCs in suspension into cell cycle arrest at G1/S, by activating the cell cycle inhibitors Cdkn1a/b and Cdkn2/a/b/c. Despite this cell cycle arrest, they can still re-enter the cell cycle upon transfer to an adhesive surface, and grow into typical mESC colonies, albeit at a lower efficiency. These results demonstrate that mESCs respond to induced c-Jun overexpression differently in suspension or adherent cultures. Our results suggest that cells in suspension may be more resistant to differentiation than when they adhere.</p></div>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"7 1","pages":"Pages 16-21"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cr.2018.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36888756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematopoiesis and microenvironment in hematological malignancies 恶性血液病的造血与微环境

Cell Regeneration Pub Date : 2018-09-01 DOI: 10.1016/j.cr.2018.08.002

Hui Cheng , Guohuan Sun , Tao Cheng

{"title":"Hematopoiesis and microenvironment in hematological malignancies","authors":"Hui Cheng , Guohuan Sun , Tao Cheng","doi":"10.1016/j.cr.2018.08.002","DOIUrl":"10.1016/j.cr.2018.08.002","url":null,"abstract":"<div><p>Adult hematopoietic stem cells (HSCs) and progenitors (HPCs) reside in the bone marrow, a highly orchestrated architecture. In the bone marrow, the process of how HSCs exert self-renewal and differentiation is tightly regulated by the surrounding microenvironment, or niche. Recent advances in imaging technologies and numerous knockout or knockin mouse models have greatly improved our understanding of the organization of the bone marrow niche. This niche compartment includes a complex network of mesenchymal stem cells (MSC), osteolineage cells, endothelial cells (arterioles and sinusoids), sympathetic nerves, nonmyelinating Schwann cells and megakaryocytes. In addition, different types of mediators, such as cytokines/chemokines, reactive oxygen species (ROS) and exosomes play a pivotal role in regulating the function of hematopoietic cells. Therefore, the niche components and the hematopoietic system make up an ecological environment that maintains the homeostasis and responds to stress, damage or disease conditions. On the other hand, the niche compartment can become a traitor that can do harm to normal hematopoietic cells under pathological conditions. Studies on the diseased bone marrow niche have only recently begun to appear in the extant literature. In this short review, we discuss the most recent advances regarding the behaviors of normal hematopoietic cells and their niche alterations in hematological malignancies.</p></div>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"7 1","pages":"Pages 22-26"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cr.2018.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36888757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

A protocol for generating induced T cells by reprogramming B cells in vivo 一种在体内通过对B细胞重编程产生诱导T细胞的方案

Cell Regeneration Pub Date : 2018-09-01 DOI: 10.1016/j.cr.2018.05.001

Qitong Weng , Fangxiao Hu , Mengyun Zhang , Yong Dong , Cui Lv , Ying Wang , Xiaofei Liu , Jinyong Wang

引用次数: 1

Cardiac progenitor reprogramming for heart regeneration 心脏再生的心脏祖细胞重编程

Cell Regeneration Pub Date : 2018-09-01 DOI: 10.1016/j.cr.2018.01.001

Behnam Ebrahimi

{"title":"Cardiac progenitor reprogramming for heart regeneration","authors":"Behnam Ebrahimi","doi":"10.1016/j.cr.2018.01.001","DOIUrl":"10.1016/j.cr.2018.01.001","url":null,"abstract":"<div><p>Myocardial infarction leads to the loss of a huge number of cardiomyocytes and the reparatory response to this phenomenon is scar tissue formation, which impairs heart function. Direct reprogramming technology offers an alternative strategy for the generation of functional cardiomyocytes not only <em>in vitro</em>, but also <em>in vivo</em> in the site of injury. Results have demonstrated cardiac tissue regeneration and improvement in heart function after myocardial infarction following local injection of vectors encoding reprogramming transcription factors or miRNAs. This shows the great potential of cardiac reprogramming technology for heart regeneration. However, in addition to cardiomyocytes, other cell types, including endothelial cells and smooth muscle cells are also required to be generated in the damaged area in order to achieve complete cardiac tissue regeneration. To this aim induced proliferative/expandable cardiovascular progenitor cells (iCPCs) appear to be an appropriate cell source, which is capable of differentiation into three cardiovascular lineages both <em>in vitro</em> and <em>in vivo</em>. In this regard, this study goes over <em>in vitro</em> and <em>in vivo</em> cardiac reprogramming technology and specifically deals with cardiac progenitor reprogramming and its potential for heart regeneration.</p></div>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"7 1","pages":"Pages 1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cr.2018.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36888753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4