Cell Regeneration最新文献

{"title":"Modelling in vitro gametogenesis using induced pluripotent stem cells: a review.","authors":"Maria Victoria Romualdez-Tan","doi":"10.1186/s13619-023-00176-5","DOIUrl":"10.1186/s13619-023-00176-5","url":null,"abstract":"<p><p>In vitro gametogenesis (IVG) has been a topic of great interest in recent years not only because it allows for further exploration of mechanisms of germ cell development, but also because of its prospect for innovative medical applications especially for the treatment of infertility. Elucidation of the mechanisms underlying gamete development in vivo has inspired scientists to attempt to recapitulate the entire process of gametogenesis in vitro. While earlier studies have established IVG methods largely using pluripotent stem cells of embryonic origin, the scarcity of sources for these cells and the ethical issues involved in their use are serious limitations to the progress of IVG research especially in humans. However, with the emergence of induced pluripotent stem cells (iPSCs) due to the revolutionary discovery of dedifferentiation and reprogramming factors, IVG research has progressed remarkably in the last decade. This paper extensively reviews developments in IVG using iPSCs. First, the paper presents key concepts from groundwork studies on IVG including earlier researches demonstrating that IVG methods using embryonic stem cells (ESCs) also apply when using iPSCs. Techniques for the derivation of iPSCs are briefly discussed, highlighting the importance of generating transgene-free iPSCs with a high capacity for germline transmission to improve efficacy when used for IVG. The main part of the paper discusses recent advances in IVG research using iPSCs in various stages of gametogenesis. In addition, current clinical applications of IVG are presented, and potential future applications are discussed. Although IVG is still faced with many challenges in terms of technical issues, as well as efficacy and safety, novel IVG methodologies are emerging, and IVG using iPSCs may usher in the next era of reproductive medicine sooner than expected. This raises both ethical and social concerns and calls for the scientific community to cautiously develop IVG technology to ensure it is not only efficacious but also safe and adheres to social and ethical norms.</p>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"12 1","pages":"33"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Generating hematopoietic cells from human pluripotent stem cells: approaches, progress and challenges.","authors":"Haiqiong Zheng, Yijin Chen, Qian Luo, Jie Zhang, Mengmeng Huang, Yulin Xu, Dawei Huo, Wei Shan, Ruxiu Tie, Meng Zhang, Pengxu Qian, He Huang","doi":"10.1186/s13619-023-00177-4","DOIUrl":"10.1186/s13619-023-00177-4","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"12 1","pages":"32"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41098345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beclin1 haploinsufficiency compromises mesenchymal stem cell-offered cardioprotection against myocardial infarction.","authors":"Xing Qin, Juanjuan Fei, Yu Duan, Asli F Ceylan, Fuyang Zhang, Jun Ren","doi":"10.1186/s13619-022-00121-y","DOIUrl":"10.1186/s13619-022-00121-y","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs)-based therapy has displayed some promises in ischemia heart diseases although its efficacy may be affected by changes in surrounding environments. This study evaluated the role of autophagy insufficiency using Beclin1 haploinsufficiency (BECN^+/-) on intra-myocardial MSC transplantation-evoked effect against myocardial infarction. Donor MSCs from C57BL/6 mice were labelled with cell-tracker CM Dil and were delivered into LV free wall adjacent to infarct region in wild-type (WT) and BECN^+/- recipient mice following ligation of left main coronary artery (MI-MSCs). Ten days following MI, myocardial function was assessed using echocardiography. Cardiomyocyte contractility and intracellular Ca²⁺ were monitored using cardiomyocytes from the area-at-risk adjacent to infarct. CM-Dil labeled cells were tracked in MSCs recipient mice using fluorescence microscopy. Lectin, Masson trichrome staining and Western blot analysis were employed to determine cardiomyocyte area, scar fibrosis, apoptosis and inflammation. MI insult triggered scar fibrosis, LV chamber dilation, decreased fractional shortening, ejection fraction, cardiomyocyte shortening, maximal velocity of shortening and relengthening as well as prolonged relengthening, which were abrogated or attenuated by MSCs therapy in WT but not BECN^+/- mice. MI decreased intracellular Ca²⁺ rise and decay in response to electrical stimuli without affecting resting intracellular Ca²⁺, which were reconciled by MSCs in WT but not BECN^+/- mice. MSCs further attenuated MI-induced mitochondrial ultrastructural injury, apoptosis, inflammation and autophagy defects in peri-infarct area in WT but not BECN^+/- mice. Collectively, our results suggested that autophagy insufficiency dampened in MSCs-elicited cardioprotection associated with dampened apoptosis and inflammation.</p>","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":"21"},"PeriodicalIF":4.0,"publicationDate":"2022-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDB1 maintains intestinal homeostasis by preventing cell cycle arrest","authors":"Lianzheng Zhao, H. Liao, Xiaodan Wang, Ye-Guang Chen","doi":"10.1186/s13619-022-00119-6","DOIUrl":"https://doi.org/10.1186/s13619-022-00119-6","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46841813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium","authors":"Haonan Li, Xiaodan Wang, Yalong Wang, Mengxian Zhang, F. Hong, Hong Wang, Along Cui, Jianguo Zhao, W. Ji, Ye-Guang Chen","doi":"10.1186/s13619-022-00118-7","DOIUrl":"https://doi.org/10.1186/s13619-022-00118-7","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43438279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mending a broken heart with novel cardiogenic small molecules","authors":"Nevan Powers, Guo N. Huang","doi":"10.1186/s13619-022-00120-z","DOIUrl":"https://doi.org/10.1186/s13619-022-00120-z","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The identification of PAX7 variants and a potential role of muscle development dysfunction in congenital scoliosis","authors":"Muchuan Wang, Ziquan Li, Sen Zhao, Zhifa Zheng, Yi‐peng Wang, G. Qiu, Zhihong Wu, N. Wu, T. Zhang, Siyi Cai","doi":"10.1186/s13619-022-00116-9","DOIUrl":"https://doi.org/10.1186/s13619-022-00116-9","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42974272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study","authors":"Changmei Niu, Liyang Wang, Dongdong Ji, Mingjun Ren, Dongxu Ke, Qiang Fu, Kaile Zhang, Xi Yang","doi":"10.1186/s13619-022-00113-y","DOIUrl":"https://doi.org/10.1186/s13619-022-00113-y","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47841717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological features of tissues and cell populations during cancer cachexia","authors":"D. Di Girolamo, S. Tajbakhsh","doi":"10.1186/s13619-022-00108-9","DOIUrl":"https://doi.org/10.1186/s13619-022-00108-9","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65859575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myogenesis controlled by a long non-coding RNA 1700113A16RIK and post-transcriptional regulation","authors":"Xin Fu, Sheng Li, Minzhi Jia, Bo Xu, Lele Yang, Ruimiao Ma, Hong Cheng, Wenjun Yang, Ping Hu","doi":"10.1186/s13619-022-00114-x","DOIUrl":"https://doi.org/10.1186/s13619-022-00114-x","url":null,"abstract":"","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42373016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}