Cell Transplantation最新文献

{"title":"The central role of fibroblasts in androgenic alopecia: A systematic umbrella review.","authors":"Shanshan Yu, Jie Ji, Yingying Wu, Jingyun Li, Hui Yan, Yongfang Xu, Yutong Shi, Jun Li","doi":"10.1177/09636897261468231","DOIUrl":"10.1177/09636897261468231","url":null,"abstract":"<p><p>BackgroundAndrogenic alopecia (AGA) is a common hair follicle miniaturization disease driven by androgens. Fibroblasts, especially dermal papilla (DP) cells, are considered the core of the pathogenesis of AGA, but there is a lack of systematic synthesis of evidence.ObjectiveTo systematically review the role of fibroblasts (DP cells and dermal sheath cells) in the pathogenesis of AGA, with a focus on androgen signaling transduction, paracrine signaling, disruption of stem cell niche, inflammation, and fibrosis.Data sourcesPubMed and Web of Science, covering the period from August 23, 2025 to May 28, 2026, including human basic research, animal model studies, and clinical pathology studies related to AGA fibroblast function. Two reviewers independently screened literature, extracted data, and evaluated the risk of bias.ResultThrough retrieval, a total of 1592 articles were identified, and 43 articles were ultimately included. DP cells consistently express high levels of androgen receptor (AR) and 5α-reductase type II. Activation of androgens (dihydrotestosterone) in DP cells leads to: (1) downregulation of Wnt/β-catenin signaling and upregulation of TGF-β/BMP pathway; (2) Changes in secretion profile (decrease in VEGF/IGF-1 and increase in DKK-1/TGF-β1); (3) DP cell aggregation behavior is impaired; (4) Destruction of hair follicle stem cell activation; (5) Inducing inflammation and fibrosis around hair follicles. The evidence for dermal sheath cells as a DP cell bank is still limited.ConclusionDP cells are the main transducers of androgen signaling in AGA, driving follicle miniaturization through multiple interrelated mechanisms. The current evidence supports DP cell dysfunction as a therapeutic strategy.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261468231"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13365651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking preclinical models for genetically engineered porcine red blood cell xenotransfusion.","authors":"Xiayu Lei, Hidetaka Hara, Tao Li, Yi Wang","doi":"10.1177/09636897261477939","DOIUrl":"10.1177/09636897261477939","url":null,"abstract":"<p><p>Genetically engineered porcine red blood cells (pRBCs) are emerging as a potential supplementary source of oxygen-carrying cells for transfusion medicine. Triple-knockout (TKO) pigs lacking major carbohydrate xenoantigens, including Gal, Neu5Gc, and Sd(a), have substantially reduced human antibody binding in vitro, and additional expression of human protective molecules such as CD55 and CD47 may further improve compatibility. However, the translational development of pRBC xenotransfusion is now constrained less by donor engineering alone than by the absence of an appropriate in vivo evaluation model. Old World nonhuman primates have historically served as essential preclinical models in xenotransplantation, but their cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH)-positive background and natural antibody repertoires may exaggerate immune barriers to TKO pRBCs that are not fully representative of humans. New World nonhuman primates, brain-dead human models, and eventually carefully regulated early-phase human studies each offer distinct advantages and limitations. In this Commentary, we argue that pRBC xenotransfusion requires a purpose-specific translational framework rather than reliance on a single preclinical model. Such a framework should integrate standardized compatibility testing, in vitro human assays, animal-based safety stress-testing, and, where ethically and legally feasible, short-term human reference models.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261477939"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13451752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen-hyaluronic acid hydrogel with embedded chondrocytes as a platform for modeling early stages of endochondral ossification <i>in vitro</i>.","authors":"Marina Malić, Martina Doubková, Šimon Pražák, Antonín Brož, Kristýna Havlíčková, Věra Jenčová, Daniel Hadraba, Lucie Bačáková","doi":"10.1177/09636897251409464","DOIUrl":"10.1177/09636897251409464","url":null,"abstract":"<p><p>Tissue engineering strategies aim to create bone models to enhance our understanding of bone development and improve bone repair. Endochondral ossification (EO), where cartilage is replaced by bone, is difficult to replicate. We developed a collagen-hyaluronic acid (HA) hydrogel system for a three-dimensional (3D) co-cultivation of human chondrocytes, human mesenchymal stem cell (hMSCs), and human umbilical vein endothelial cells (HUVECs) to model the early stages of EO. To our knowledge, this specific tri-culture configuration within such a hydrogel composition has not been previously reported, providing a novel platform for studying EO-related cellular and matrix interactions <i>in vitro.</i> Differentiation and migration of hMSCs and HUVECs were studied in collagen I (Col I) hydrogels, with or without the addition of collagen II (Col II), HA, and chondrocytes. Col II and HA enhanced cell osteogenic differentiation, as documented by an increased <i>COL1A1</i> and <i>ALPL</i> gene expression and enhanced alkaline phosphatase activity. Chondrocytes further promoted osteogenic cell differentiation and temporarily enhanced hypertrophic and angiogenic signaling as evidenced by elevated <i>MMP13</i> and <i>VEGFA</i> expression. Furthermore, elevated <i>SOX9</i> expression and Alcian blue staining indicated a chondrogenic-supportive environment. These findings highlight the synergistic effects of osteogenic and chondrogenic signals, emphasizing the potential of combining Col I and Col II, HA, and essential cell types in hydrogels to optimize bone and cartilage tissue engineering.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897251409464"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12905078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146194140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term outcomes of autologous cell transplantation for patients with non-TAO autoimmune disease-related critical limb ischemia.","authors":"Hao Liu, Lingwei Zou, Yuan Fang, Gang Fang, Yifan Liu, Xiaolang Jiang, Bin Chen, Zheng Wei, Shiyang Gu, Peng Liu, Weiguo Fu, Tianyue Pan, Zhihui Dong","doi":"10.1177/09636897261424576","DOIUrl":"10.1177/09636897261424576","url":null,"abstract":"<p><p>Autoimmune disease-related critical limb ischemia (AD-CLI) represents a significant portion of no-option critical limb ischemia (NO-CLI). Despite the demonstrated safety and efficacy of cell transplantation for NO-CLI, most studies focused on thromboangiitis obliterans (TAO). There remains a scarcity of reports on cell transplantation for AD-CLI induced by systemic lupus erythematosus (SLE), hypereosinophilic syndrome (HES), and so on. From May 2011 to May 2024, 22 patients with 36 ischemic limbs were enrolled. The primary outcome was amputation, whereas secondary outcomes included toe-brachial index (TBI), Wong-Baker Faces Pain Rating Scale (WBFPS), transcutaneous oxygen pressure (TcPO₂), and so on. Among them, 8 had SLE, 11 had HES, and 3 had eosinophilic granulomatous polyangiitis (EGPA). During the follow-up, one EGPA patient died, two patients underwent major amputation, and five underwent minor amputation. The 2-year major and total amputation-free survival rates were 86.4% and 72.7%, respectively. Critical limb ischemia (CLI) relief was observed in 14 patients, with a 1-year cumulative CLI-free survival rate of 61.0%. Significant improvements were noted in postoperative TBI and WBFPS. Autologous cell transplantation showed satisfactory safety and efficacy outcomes for non-TAO AD-CLI patients. Validation of the conclusions awaits more evidence based on the long-term outcomes of a larger number of patients.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261424576"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrence of focal segmental glomerulosclerosis: An updated review of pathophysiology, biomarkers, and therapeutic strategies.","authors":"Zachary Oatley, Danny Jaber, Nikhil Rayarakula, Thomas Guirguis, Layth Khawaja, Siddharth Bhindwallam, Marisol Aguirre, Jaime Manuel Restrepo, Rupesh Raina","doi":"10.1177/09636897261417049","DOIUrl":"10.1177/09636897261417049","url":null,"abstract":"<p><p>Focal segmental glomerulosclerosis (FSGS) is one of the major causes of nephrotic syndrome, which can progress to end-stage renal disease, leading to kidney transplantation. Following renal transplantation, recurrence of FSGS (rFSGS) occurs in 30%-40% of patients with a high risk of graft loss. rFSGS typically presents with nephrotic-range proteinuria within days after post-transplantation. This review summarizes pathophysiology, biomarkers, and therapeutic strategies for rFSGS. Monogenic causes of FSGS, such as those caused by APOL1 mutation, show variable recurrence, while NPHS2 and ACTN4 show low recurrence of FSGS. Evidence suggests that idiopathic or primary FSGS is strongly associated with rFSGS, owing to podocyte structural damage caused by circulating permeability factors or immune dysfunction. Recent advances have identified biomarkers such as anti-nephrin antibodies, anti-CD40 antibodies, soluble tumor necrosis factor receptor 2 (sTNFR2), and soluble urokinase-type plasminogen activator receptor (suPAR) that help in early detection of recurrent FSGS. Post-transplant monitoring includes measuring urine protein-to-creatinine ratio (UPCR) and 24-h urine protein excretion, and a kidney biopsy. Preventive strategies, although including plasmapheresis and rituximab, show limited benefit and are not recommended for routine prophylaxis. Treatment options include plasmapheresis, immunoadsorption, and immunosuppressive drugs such as cyclophosphamide, rituximab, or calcineurin inhibitors. Recurrent FSGS is a clinical challenge with its multifactorial pathogenesis. Incorporating strategies such as genetic testing, risk stratification, and early detection with the help of biomarkers and early treatment can induce remission and preserve graft survival. Despite these advances, large prospective studies are still required for standardizing prevention and management strategies for rFSGS.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261417049"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13039636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CNTN1/Gilz pathway mediates the therapeutic effect of mesenchymal stem cells on <i>Echinococcus multilocularis</i>-induced hepatic inflammation and fibrosis.","authors":"Shigui Chong, Wenhua Gao, Shixin Lei, Yulin He, Yumin Zhao","doi":"10.1177/09636897261487114","DOIUrl":"10.1177/09636897261487114","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) exhibit potent immunomodulatory properties, yet their precise mechanisms in alleviating Echinococcus multilocularis-induced hepatic inflammation and fibrosis in Alveolar Echinococcosis (AE) remain elusive. This study aimed to elucidate the immunoregulatory mechanisms underlying MSCs therapy in AE. Using an AE mouse model, we demonstrated that MSCs administration significantly ameliorated liver histopathology, attenuated fibrosis, and restored liver function. Molecular analyses revealed that AE infection promoted a pro-inflammatory profile (elevated RORγt and IFN-γ) while suppressing regulatory factors (FOXP3, TGF-β1, IL-10, CNTN1, and Gilz), effects that were robustly reversed by MSCs treatment. In vitro, co-culture of CNTN1-knockdown MSCs with E. multilocularis antigen-stimulated CD4⁺ T cells demonstrated that CNTN1 deficiency impaired MSCs-mediated immunoregulation, decreased IL-10 secretion, and disrupted the Th17/Treg balance. Mechanistically, dual-luciferase reporter assays confirmed that CNTN1 directly activates the Gilz promoter. Collectively, our findings identify the CNTN1/Gilz signaling axis as an essential mediator of MSCs immunomodulation, providing novel mechanistic insights into the therapeutic efficacy of MSCs against E. multilocularis-induced hepatic injury.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261487114"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13554678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of concern: \"The melatonin analog IQM316 may induce adult hippocampal neurogenesis and preserve recognition memories in mice\".","authors":"","doi":"10.1177/09636897261468083","DOIUrl":"10.1177/09636897261468083","url":null,"abstract":"","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261468083"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13380686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148469142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and preliminary efficacy of allogeneic umbilical cord-derived mesenchymal stem cells administered via the hepatic artery in patients with liver cirrhosis: A phase I open-label trial.","authors":"Liem Thanh Nguyen, Hoang-Phuong Nguyen, Hang Thi Bui, Quyen Thi Nguyen, Phan Van Nguyen, Hien Thi Thu Ha","doi":"10.1177/09636897261449975","DOIUrl":"10.1177/09636897261449975","url":null,"abstract":"<p><p>Liver cirrhosis causes substantial morbidity, and options beyond transplantation are limited. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) may support liver repair, but their delivery via the hepatic artery has not been evaluated. This study assessed the safety and exploratory efficacy of allogeneic UC-MSCs infusion in cirrhosis. Twenty patients with liver cirrhosis entered a single-center open-label pilot trial in Hanoi, Vietnam, between 2020 and 2023; 17 completed follow-up and were included in exploratory analyses. All received a single hepatic artery infusion of UC-MSCs at 1 × 10⁶ cells/kg and were assessed at baseline, 3, 6, and 12 months for liver function, Model for End-stage Liver Disease (MELD) and Child-Pugh scores, Chronic Liver Disease Questionnaire (CLDQ), and adverse events. No serious adverse events occurred; mild events were self-limited. Albumin increased at 3 and 6 months (<i>P</i> = 0.048; <i>P</i> = 0.027). Bilirubin, liver enzymes, coagulation, and MELD remained stable. Child-Pugh score decreased transiently at 3 months (<i>P</i> = 0.024). CLDQ increased across most domains, except systemic symptoms. Hepatic artery infusion of UC-MSCs was safe and well tolerated, with observed increases in albumin and in most CLDQ domains, while other parameters remained stable. Findings are exploratory because of the small sample size and lack of a control group and therefore require confirmation in larger, controlled studies.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261449975"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13213112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148027489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in bioactive bone graft materials and bone marrow enrichment techniques for bone defect treatment.","authors":"Zhezheng Shen, Xueyuan Li, Zijun Ye, Yuhao Zhou, Di Tu, Zongying Tian, Wei Qian","doi":"10.1177/09636897261445513","DOIUrl":"10.1177/09636897261445513","url":null,"abstract":"<p><p>Bone defects arise from trauma, infection, tumor resection, or congenital deformities, with large defects often progressing to nonunion. Autologous bone grafting is considered the gold standard therapy due to its osteoconductive, osteoinductive, and osteogenic properties, but it is limited by insufficient graft volume and donor-site complications. Allogeneic grafts and early synthetic bone substitutes partially addressed these limitations but typically provided only osteoconductive function and performed poorly in complex microenvironments. Bone tissue engineering utilizes bioactive scaffolds, cells, and factors to promote bone repair through mechanical support, osteogenic regulation, and cellular adhesion/proliferation/differentiation mechanisms. This review synthesizes current evidence on bioactive scaffold materials and bone marrow enrichment strategies for bone defect repair within a scaffold-bone marrow coupling framework. It also highlights key roles of pore architecture, angiogenesis, osteoimmunomodulation, and defect-specific material selection, while critically discussing current limitations, evidence gaps, and future translational directions.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261445513"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13219946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of functional human islets from pancreatectomy remnants: Determinants of yield and functionality.","authors":"Karl Herbert Hillebrandt, Theresa Lohmann, Luna Haderer, Simon Moosburner, Matthäus Felsenstein, Lene Änne Böhne, Alexander Arnold, Anja Reutzel-Selke, Johann Pratschke, Thomas Malinka, Igor M Sauer, Eriselda Keshi","doi":"10.1177/09636897261452371","DOIUrl":"10.1177/09636897261452371","url":null,"abstract":"<p><p>Human pancreatic islets are essential for studies in β-cell biology, cell transplantation, and tissue engineering, yet access to viable human islets remains limited because conventional isolation protocols primarily rely on whole pancreases from deceased donors. Surgical pancreatectomy specimens may represent an accessible alternative source, but factors influencing successful isolation and functional preservation remain poorly defined. In this study, we evaluated a standardized method for isolating human islets from pancreatic tissue obtained from surgical pancreatectomy specimens and investigated patient-, specimen-, and surgery-related factors affecting islet yield and functionality. Between March and October 2024, 50 consecutive islet isolations were performed from pancreatic specimens obtained during surgical resections. Following enzymatic digestion, tissue fractions were cultured for 24 h before handpicking of morphologically intact islets. Islet yield was quantified as islet equivalents (IEQ), and functional integrity was assessed by glucose-stimulated insulin secretion assays on culture days 1, 3, and 5. A mean yield of 6690 IEQ/g pancreatic tissue was obtained (range 0-56,500 IEQ/g). Exploratory analyses suggested potential associations between islet yield and factors such as younger patient age, shorter surgical duration, and preserved pancreatic parenchyma. However, these findings should be interpreted cautiously given the variability of the specimens and the exploratory design of the study. These findings support the feasibility of using surgical pancreatectomy specimens as an accessible source of functional human islets for experimental and translational research.</p>","PeriodicalId":9721,"journal":{"name":"Cell Transplantation","volume":"35 ","pages":"9636897261452371"},"PeriodicalIF":3.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13272981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148263397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}