Cell calcium最新文献_第9页

Astrocytic chloride regulates brain function in health and disease 星形胶质细胞氯化物调节健康和疾病中的大脑功能

IF 4 2区生物学

Cell calcium Pub Date : 2024-02-07 DOI: 10.1016/j.ceca.2024.102855

Verena Untiet

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引用次数: 0

MLKL overexpression leads to Ca2+ and metabolic dyshomeostasis in a neuronal cell model 在神经元细胞模型中，MLKL 过表达会导致 Ca2+ 和代谢失衡

IF 4 2区生物学

Cell calcium Pub Date : 2024-02-06 DOI: 10.1016/j.ceca.2024.102854

Sathyaseelan S Deepa , Nidheesh Thadathil , Jorge Corral , Sabira Mohammed , Sophia Pham , Hadyn Rose , Michael T Kinter , Arlan Richardson , Carlos Manlio Díaz-García

{"title":"MLKL overexpression leads to Ca2+ and metabolic dyshomeostasis in a neuronal cell model","authors":"Sathyaseelan S Deepa , Nidheesh Thadathil , Jorge Corral , Sabira Mohammed , Sophia Pham , Hadyn Rose , Michael T Kinter , Arlan Richardson , Carlos Manlio Díaz-García","doi":"10.1016/j.ceca.2024.102854","DOIUrl":"10.1016/j.ceca.2024.102854","url":null,"abstract":"<div>The necroptotic effector molecule MLKL accumulates in neurons over the lifespan of mice, and its downregulation has the potential to improve cognition through neuroinflammation, and changes in the abundance of synaptic proteins and enzymes in the central nervous system. Notwithstanding, direct evidence of cell-autonomous effects of MLKL expression on neuronal physiology and metabolism are lacking. Here, we tested whether the overexpression of MLKL in the absence of cell death in the neuronal cell line Neuro-2a recapitulates some of the hallmarks of aging at the cellular level. Using genetically-encoded fluorescent biosensors, we monitored the cytosolic and mitochondrial Ca2+ levels, along with the cytosolic concentrations of several metabolites involved in energy metabolism (lactate, glucose, ATP) and oxidative stress (oxidized/reduced glutathione). We found that MLKL overexpression marginally decreased cell viability, however, it led to reduced cytosolic and mitochondrial Ca2+ elevations in response to Ca2+ influx from the extracellular space. On the contrary, Ca2+ signals were elevated after mobilizing Ca2+ from the endoplasmic reticulum. Transient elevations in cytosolic Ca2+, mimicking neuronal stimulation, lead to higher lactate levels and lower glucose concentrations in Neuro-2a cells when overexpressing MLKL, which suggest enhanced neuronal glycolysis. Despite these alterations, energy levels and glutathione redox state in the cell bodies remained largely preserved after inducing MLKL overexpression for 24–48 h. Taken together, our proof-of-concept experiments are consistent with the hypothesis that MLKL overexpression in the absence of cell death contributes to both Ca2+ and metabolic dyshomeostasis, which are cellular hallmarks of brain aging.</div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"119 ","pages":"Article 102854"},"PeriodicalIF":4.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139819369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPV3 returns with a pleasant feeling of warmth TRPV3 带着温暖的愉悦感回归

IF 4 2区生物学

Cell calcium Pub Date : 2024-02-01 DOI: 10.1016/j.ceca.2024.102853

Michael Schaefer

引用次数: 0

Astrocytic Na+, K+ ATPases in physiology and pathophysiology 生理和病理生理学中的星形胶质细胞 Na+、K+ ATP 酶

IF 4 2区生物学

Cell calcium Pub Date : 2024-01-28 DOI: 10.1016/j.ceca.2024.102851

Daniela Pietrobon , Fiorenzo Conti

{"title":"Astrocytic Na+, K+ ATPases in physiology and pathophysiology","authors":"Daniela Pietrobon , Fiorenzo Conti","doi":"10.1016/j.ceca.2024.102851","DOIUrl":"10.1016/j.ceca.2024.102851","url":null,"abstract":"<div>The Na+, K+ ATPases play a fundamental role in the homeostatic functions of astrocytes. After a brief historic prologue and discussion of the subunit composition and localization of the astrocytic Na+, K+ ATPases, the review focuses on the role of the astrocytic Na+, K+ pumps in extracellular K+ and glutamate homeostasis, intracellular Na+ and Ca2+ homeostasis and signaling, regulation of synaptic transmission and neurometabolic coupling between astrocytes and neurons. Loss-of-function mutations in the gene encoding the astrocytic α2 Na+, K+ ATPase cause a rare monogenic form of migraine with aura (familial hemiplegic migraine type 2). On the other hand, the α2 Na+, K+ ATPase is upregulated in spinal cord and brain samples from amyotrophic lateral sclerosis and Alzheimer disease patients, respectively. In the last part, the review focuses on i) the migraine relevant phenotypes shown by familial hemiplegic migraine type 2 knock-in mice with 50 % reduced expression of the astrocytic α2 Na+, K+ ATPase and the insights into the pathophysiology of migraine obtained from these genetic mouse models, and ii) the evidence that upregulation of the astrocytic α2 Na+, K+ ATPase in mouse models of amyotrophic lateral sclerosis and Alzheimer disease promotes neuroinflammation and contributes to progressive neurodegeneration.</div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"118 ","pages":"Article 102851"},"PeriodicalIF":4.0,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000095/pdfft?md5=d52257412e775f1774f325fe6af0143e&pid=1-s2.0-S0143416024000095-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139584682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPM2 - An adjustable thermostat TRPM2 - 可调节的恒温器

IF 4 2区生物学

Cell calcium Pub Date : 2024-01-11 DOI: 10.1016/j.ceca.2024.102850

Ádám Bartók , László Csanády

{"title":"TRPM2 - An adjustable thermostat","authors":"Ádám Bartók , László Csanády","doi":"10.1016/j.ceca.2024.102850","DOIUrl":"10.1016/j.ceca.2024.102850","url":null,"abstract":"<div>The Transient Receptor Potential Melastatin 2 (TRPM2) channel is a homotetrameric ligand-gated cation channel opened by the binding of cytosolic ADP ribose (ADPR) and Ca2+. In addition, strong temperature dependence of its activity has lately become a center of attention for both physiological and biophysical studies. TRPM2 temperature sensitivity has been affirmed to play a role in central and peripheral thermosensation, pancreatic insulin secretion, and immune cell function. On the other hand, a number of different underlying mechanisms have been proposed from studies in intact cells. This review summarizes available information on TRPM2 temperature sensitivity, with a focus on recent mechanistic insight obtained in a cell-free system. Those biophysical results outline TRPM2 as a channel with an intrinsically endothermic opening transition, a temperature threshold strongly modulated by cytosolic agonist concentrations, and a response steepness greatly enhanced through a positive feedback loop generated by Ca2+ influx through the channel's pore. Complex observations in intact cells and apparent discrepancies between studies using in vivo and in vitro models are discussed and interpreted in light of the intrinsic biophysical properties of the channel protein.</div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"118 ","pages":"Article 102850"},"PeriodicalIF":4.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000083/pdfft?md5=c0d5c58ff1c531cb84ffdeb70356250c&pid=1-s2.0-S0143416024000083-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139468974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trace metals and astrocytes physiology and pathophysiology 痕量金属与星形胶质细胞的生理学和病理生理学

IF 4 2区生物学

Cell calcium Pub Date : 2024-01-04 DOI: 10.1016/j.ceca.2024.102843

Baoman Li , Weiyang Yu , Alexei Verkhratsky

引用次数: 0

TRPV2 inhibitor tranilast prevents atrial fibrillation in rat models of pulmonary hypertension TRPV2 抑制剂氨曲司特能预防肺动脉高压大鼠模型中的心房颤动

IF 4 2区生物学

Cell calcium Pub Date : 2024-01-01 DOI: 10.1016/j.ceca.2023.102840

Tianxin Ye , Zhuonan Song , Yunping Zhou , Zhangchi Liu , Yi Yu , Fangcong Yu , Yanan Chu , Jiaran Shi , Longbo Wang , Cui Zhang , Xin Liu , Bo Yang , Jinxiu Yang , Xingxiang Wang

{"title":"TRPV2 inhibitor tranilast prevents atrial fibrillation in rat models of pulmonary hypertension","authors":"Tianxin Ye , Zhuonan Song , Yunping Zhou , Zhangchi Liu , Yi Yu , Fangcong Yu , Yanan Chu , Jiaran Shi , Longbo Wang , Cui Zhang , Xin Liu , Bo Yang , Jinxiu Yang , Xingxiang Wang","doi":"10.1016/j.ceca.2023.102840","DOIUrl":"10.1016/j.ceca.2023.102840","url":null,"abstract":"<div>Atrial fibrillation (AF) is common in pulmonary hypertension (PH), whereas the mechanisms and treatments remain to be explored. TRPV2 regulates the structure and function of the cardiovascular system; however, little attention has been given to its role in AF. This study was to determine whether TRPV2 was involved in PH-induced AF and the effects of TRPV2 inhibitor tranilast on AF in rat models of PH. Monocrotaline (MCT) and SU5416/hypoxia (SuHx)-induced PH models were performed to detect atrial electrophysiological parameters. Daily tranilast (a TRPV2 inhibitor) or saline was given starting 1 day before PH establishment. PH increased the susceptibility to AF, with TRPV2 up-regulated in the right atria. Compared to PH rats, tranilast reduced AF inducibility and the prolongations of ERP and APD; mitigated cardiopulmonary remodeling and the increases in P-wave duration and P-R interval; partially reversed the down-regulation of ion channels such as Cav1.2, Nav1.5, Kv4.3, Kv4.2, Kv1.5, Kir2.1, Kir3.1, Kir3.4 as well as connexin (Cx) 40 and Cx43; improved right atrial (RA) fibrosis, enlargement, and myocardial hypertrophy; decreased the accumulation of inflammatory cells; down-regulated inflammatory indicators such as TNF-α, IL-1β, CXCL1, and CXCL2; and inhibited the activation of the PI3K-AKT-NF-κB signaling pathway. Our results reveal that TRPV2 participates in PH-induced AF, and TRPV2 inhibitor tranilast prevents PH-induced RA remodeling. TRPV2 might be a promising target for PH-induced AF.</div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"117 ","pages":"Article 102840"},"PeriodicalIF":4.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139020366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

“Gatekeepers of Heaven” breaking bad "天国守门人 "坏了

IF 4 2区生物学

Cell calcium Pub Date : 2023-12-21 DOI: 10.1016/j.ceca.2023.102842

Hwei Ling Ong, Indu S. Ambudkar

引用次数: 0

Aromatically stacking the odds in favour of increased ORAI1 activation 从芳香学角度看，增加 ORAI1 激活的可能性更大。

IF 4 2区生物学

Cell calcium Pub Date : 2023-12-21 DOI: 10.1016/j.ceca.2023.102841

Peter B. Stathopulos , Mitsuhiko Ikura

引用次数: 0

“Gatekeepers of heaven” breaking bad "天国守门人 "坏了