CARTILAGE最新文献

{"title":"Cartilage Injuries of the Ankle: New Beginnings.","authors":"Gino M M J Kerkhoffs, John G Kennedy, Mats Brittberg, Jari Dahmen","doi":"10.1177/19476035251361353","DOIUrl":"10.1177/19476035251361353","url":null,"abstract":"","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251361353"},"PeriodicalIF":2.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes After Initial Non-Operative Treatment of Osteochondral Lesions of the Talus (OLT) in Skeletally Immature Patients: A Cross-Sectional Study.","authors":"Jason A H Steman, Tristan M F Buck, Jari Dahmen, Peter A A Struijs, Sjoerd A S Stufkens, Gino M M J Kerkhoffs","doi":"10.1177/19476035251357214","DOIUrl":"10.1177/19476035251357214","url":null,"abstract":"<p><p>IntroductionLiterature on treatment outcomes in skeletally immature patients with osteochondral lesions of the talus (OLT) is scarce. As the healing of an OLT may be fundamentally different in a skeletally immature patient, more evidence is required focusing on this specific patient group. The primary aim of this study is to assess the conversion to surgery rate after initial non-operative management in skeletally immature patients with an OLT. The secondary aims of the present study are to assess and compare the clinical outcomes and reoperations after both non-operative and surgical treatment strategies at a mid- to long-term follow-up.MethodsAll skeletally immature patients at the moment of initial treatment, treated for their primary or non-primary OLT with a minimum follow-up duration of 2 years, were included in this study. Patients with concomitant injuries were excluded. All patients started with non-operative management. In case of failure of non-operative management, patients converted to Bone Marrow Stimulation (BMS) or fixation. The primary outcome was the conversion to surgery rate after initial non-operative management. Secondary outcomes consist of reoperations at mature and immature age, pain during weight bearing, measured by the numeric rating scale (NRS), NRS of pain during rest, NRS during stair climbing, Berndt and Harty outcome question, Foot and Ankle Outcome Score (FAOS) and Short Form-36 (SF-36) and the patient satisfaction rate regarding the received treatment.ResultsA total of 52 patients, 54% female, mean age of 13.6 years, were included in this study. Median follow-up duration was 81 months (range = 24-265 months). Seventeen patients received non-operative treatment as final treatment. In total, 35 (67%) out of 52 patients required surgical treatment after initial non-operative management, of which 14 underwent BMS and 20 had fixation while skeletally immature, 1 patient that had surgical treatment as an adult was excluded for further analysis. The median NRS of pain during weight bearing was 1 (interquartile range [IQR] = 0-2), 1 (IQR = 0-3), and 0 (IQR = 0-0.5) in the (sustained) non-operative, BMS, and fixation groups, respectively (<i>P</i> < 0.012). No significant differences in clinical outcomes between the different treatment groups could be observed. No complications occurred after surgical treatment. Reoperation rates were 21% and 20% in the BMS and fixation groups, respectively.ConclusionsThe most important finding of this study is that 67% of the patients receiving initial non-operative management for OLTs ultimately required surgery.Level of evidenceLevel III, cross-sectional comparative study.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251357214"},"PeriodicalIF":2.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthroscopic Non-Concentrated Autologous Iliac Bone Marrow Stimulation Shows Stable Medium-Term Clinical and Radiological Outcomes for Small Cystic Talar Osteochondral Lesions.","authors":"Boyu Zheng, Fei Yan, Yanjun Zhong, Shijun Wei, Helin Wu, Feng Xu","doi":"10.1177/19476035251356380","DOIUrl":"10.1177/19476035251356380","url":null,"abstract":"<p><p>ObjectiveTo evaluate radiological, short-term, and medium-term clinical outcomes of arthroscopic non-concentrated iliac bone marrow stimulation (BMS) for small talar cystic osteochondral lesions of the talus (OLTs).DesignForty-three cases underwent this modified BMS between 2014 and 2019 were evaluated. Clinical outcomes were assessed by the Foot and Ankle Ability Measure (FAAM) Activities of Daily Living (ADL) and Sports Subscales (SS). Regenerated tissue was evaluated with the Magnetic Resonance Observation of Cartilage Repair Tissue scales (MOCART-2.0). Subgroup analysis based on locations and concomitant anterior talofibular ligament (ATFL) injuries.ResultsThe average diameter and depth of cysts were 6.97 ± 1.53 mm and 5.47 ± 1.10 mm, respectively. At a mean follow-up of 57.02 ± 19.61 months, FAAM-ADL and FAAM-SS improved significantly (45.65 ± 4.56 to 74.77 ± 8.03 and 12.63 ± 1.87 to 26.67 ± 3.41, respectively). From short-term to medium-term, FAAM-ADL revealed a minor decline (75.53 ± 7.76 vs. 74.77 ± 8.03, <i>P</i> = 0.421); FAAM-SS improved (25.37 ± 3.51 vs. 26.67 ± 3.41, <i>P</i> = 0.089). Medial lesions demonstrated favorable outcomes compared to lateral lesions [FAAM-ADL (77.04 ± 7.23 vs. 70.75 ± 8.10, <i>P</i> = 0.013), FAAM-SS (28.08 ± 2.40 vs. 24.19 ± 3.51, <i>P</i> < 0.001), and MOCART-2.0 (85.19 ± 11.27 vs. 71.88 ± 11.09, <i>P</i> < 0.001)]. Lateral lesions indicated higher rates of major hypertrophy (56.25% vs. 7.69%) and split-like defects (56.25% vs. 15.38%). The ATFL injuries did not significantly influence revision rates (15.8% vs. 4.2%, <i>P</i> = 0.439).ConclusionsArthroscopic non-concentrated iliac BMS demonstrated stable outcomes for small cystic OLTs. Lateral lesions were associated with inferior subjective scores and relatively higher rates of irregular fibrocartilage.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251356380"},"PeriodicalIF":2.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Vascular Endothelial Growth Factor Concentration on the Short-term Efficacy of Platelet-Rich Plasma (PRP) Therapy for Knee Osteoarthritis.","authors":"Nanako Yamamoto, Yoshitomo Saita, Yohei Kobayashi, Takanori Wakayama, Sayuri Uchino, Yasumasa Momoi, Ryosuke Nakajima, Takaya Ohtaki, Haruka Kaneko, Muneaki Ishijima","doi":"10.1177/19476035251352178","DOIUrl":"10.1177/19476035251352178","url":null,"abstract":"<p><p>IntroductionThis study investigates the association between vascular endothelial growth factor (VEGF) levels and platelet-rich plasma (PRP) treatment outcomes, as well as the role of other cytokines in symptom improvement.MethodsThirty-nine patients with knee osteoarthritis (KOA) who underwent PRP therapy were analyzed. Cytokine and growth factor levels in PRP were measured, and clinical outcomes were assessed using the visual analog scale (VAS) and the Knee Injury and Osteoarthritis Outcome Score (KOOS) before and 1 month after a single intra-articular PRP injection. Correlations between cytokine levels and clinical improvements were evaluated.ResultsAge correlated positively with C-X-C motif chemokine ligand 9 (CXCL9) (r = 0.50, p < 0.001). Body mass index (BMI) correlated negatively with interleukin-10 (IL-10) and positively with interleukin-18 (IL-18). Elevated IL-18 levels correlated with worse KOOS-Activities of Daily Living (ADL) improvements (r = -0.410, <i>P</i> = 0.01), linking obesity, inflammation, and reduced PRP efficacy. While VEGF showed no association with patient background, higher VEGF levels correlated with poorer VAS score improvements (r = -0.381, <i>P</i> = 0.017), suggesting reduced PRP efficacy. A VEGF cut-off of 120 pg/ml identified non-responders with 82.6% sensitivity, 56.2% specificity, and an area under the curve (AUC) of 0.71. Among patients with VEGF ≥120 pg/ml, the response rate was 26.9%, while those with VEGF <120 pg/ml had 75%.ConclusionsHigher VEGF concentrations in PRP were associated with reduced short-term clinical efficacy in patients with knee osteoarthritis. VEGF may serve as a predictive biomarker for PRP treatment response.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251352178"},"PeriodicalIF":2.7,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12228642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of Human Induced Pluripotent Stem Cells Toward Implantable Chondroprogenitor Cells.","authors":"Josefine Ekholm, Kristina Vukusic, Camilla Brantsing, Georgina Shaw, Fazal Ur Rehman Bhatti, Stina Simonsson, Anna Falk, Mary Murphy, Victoria Rotter Sopasakis, Anders Lindahl","doi":"10.1177/19476035251351713","DOIUrl":"10.1177/19476035251351713","url":null,"abstract":"<p><p><i>Background.</i> Post-traumatic chondral and osteochondral lesions can be treated with autologous chondrocyte implantation (ACI), but the high cost of autologous cell expansion under strict Good Manufacturing Practice (GMP) regulations limits patient access. Stem cell-based advanced therapy medicinal products (ATMPs) offer more cost-effective alternatives, with human induced pluripotent stem cells (iPSC) showing great promise due to their expandability, low immunogenicity, commercialization potential, and fewer ethical concerns. <i>Aim.</i> To develop a protocol to direct iPSC through a mesenchymal stage into chondroprogenitors (iCHOp), resembling autologous chondroprogenitor cells used in ACI. <i>Methods.</i> The derived chondroprogenitor cells were expanded in monolayer and in 3-dimensional (3D) cultures and subsequently analyzed using transcriptomic profiling via RNA sequencing and reverse transcription quantitative polymerase chain reaction and compared with ACI chondrocytes. <i>Results.</i> Transcriptomic profiling confirmed successful differentiation, with iCHOp showing 83% similarity to ACI chondrocytes. Further 3D culture maturation led to upregulation of chondrogenesis-related genes and activation of cartilage-specific pathways. Histological analysis confirmed extracellular matrix production, including proteoglycans, collagen, and versican. Furthermore, the protocol's reproducibility was demonstrated using 3 distinct iPSC lines, successfully expanded in both serum-containing and defined serum-free media. <i>Conclusion.</i> Our optimized approach yields iCHOp with phenotypes closely matching ACI chondrocytes, offering a solid foundation for further development and potential clinical applications in cartilage repair.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251351713"},"PeriodicalIF":2.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medial Meniscus Posterior Horn Horizontal Tears are Associated with Knee Posteromedial Impinging Structures Inducing Shearing Forces in Patients with Meniscus Degeneration.","authors":"Sumin Lim, Jun Young Chung, Jae-Young Park, Hee-Woong Yun, Sujin Noh, Do Young Park","doi":"10.1177/19476035251347728","DOIUrl":"10.1177/19476035251347728","url":null,"abstract":"<p><p>PurposeThis study aimed to identify potential impinging and shear stress-inducing factors in knees with medial meniscus posterior horn horizontal tears (MMPHHT) using magnetic resonance imaging (MRI) in middle-aged patients with meniscal degeneration.Materials and MethodsWe retrospectively analyzed and compared consecutive patients with MMPH signal changes or MMPHHT on MRI from January 2015 to January 2022. After 1:1 propensity score matching, 80 patients in each group were analyzed. Bony impinging factors, including the femoral condylar offset ratio, the ratio of posterior condylar offset (PCO) to tibial width, posterior medial tibial plateau concavity, and the medial tibial slope, were assessed. Soft tissue impinging factors, such as the MMPH coverage ratio, presence of medial femoral condyle focal cartilage defects or posterior tibial osteophytes, were also analyzed.ResultsDemographic data did not differ between MMPHHT and MMPH signal change groups. MMPHHT group showed increased medial tibial slope (5.33 ± 2.05° vs 4.21 ± 2.58°, <i>P</i> = .003), higher incidence of posterior medial tibial plateau concavity (<i>P</i> = .040), greater MMPH coverage ratio (0.43% ± 0.05% vs 0.41% ± 0.04%, <i>P</i> = .022), and more posterior tibial osteophytes (<i>P</i> = .012). Multivariate logistic regression identified higher medial tibial slope (OR = 1.288, <i>P</i> = .016), MMPH coverage ratio (OR = 1.369 × 10⁴, <i>P</i> = .020), and posterior tibial osteophytes (OR = 4.525, <i>P</i> = .009) as independent factors associated with MMPHHT.ConclusionIn conclusion, we have determined several anatomical contributing factors related to MMPHHT. Such factors may be useful in understanding the progression of meniscus degeneration in early OA knees. Furthermore, addressing correctable factors during surgery such as tibia slope correction or osteophytectomy may improve repair results of MMPHHT in the future.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251347728"},"PeriodicalIF":2.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Collagen-Based Filler for Trapeziometacarpal Osteoarthritis: Long-Term Follow-Up and Future Applications.","authors":"Massimo Corain, Niccolò Faccioli, Umberto Lavagnolo","doi":"10.1177/19476035251354926","DOIUrl":"10.1177/19476035251354926","url":null,"abstract":"<p><p>BackgroundTrapeziometacarpal osteoarthritis (TMO) is a prevalent degenerative condition. While conservative treatments such as physiotherapy, drugs, and corticosteroid or hyaluronic acid injections offer symptomatic relief, their long-term efficacy remains debated. A recent study has explored collagen-based fillers as an alternative, but long-term clinical outcomes are still under investigation.MethodsThis study enrolled 64 patients diagnosed with TMO, stratified into 2 groups based on the Eaton-Littler classification (grade 1-2: group A; grade 3-4: group B). All patients received a percutaneous intra-articular injection of a cell-free collagenic hydrogel under ultrasound guidance. Outcomes were assessed more than 2 years using the Numeric Rating Scale (NRS) for pain, Jamar and Pinch tests for grip strength, and the Disability of the Arm, Shoulder, and Hand (DASH) questionnaire.ResultsIn both groups, all studied variables demonstrated a significant improvement (<i>P</i> < 0.001) that was sustained in the long term. Notably, greater improvement was observed in strength tests for Group A patients and in the DASH score for Group B patients. The most substantial improvement occurred between 2 and 6 months post-procedure. No adverse events were reported.ConclusionCollagen-based filler injections provide long-term pain relief and functional improvement in TMO, representing a promising minimally invasive treatment option.Trial registry name:NCT06881186.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251354926"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct Fixation of Acute Chondral-Only Fragments in Young Patients.","authors":"Paul B Walker, Guillermo Araujo, Mathangi Sridharan, Eghosa Edogun, William L Sheppard, Thomas J Kremen, Peter D Fabricant, Kristofer J Jones","doi":"10.1177/19476035251351781","DOIUrl":"10.1177/19476035251351781","url":null,"abstract":"<p><p>IntroductionCartilage lesions of the knee frequently result from acute traumatic injuries and pose significant challenges, particularly in young and active patients. While many involve osteochondral lesions, isolated chondral defects also occur. Traditional treatment focuses on fixation when viable subchondral bone is present; however, managing chondral-only lesions remains controversial due to limited intrinsic healing capacity.MethodsA systematic review was conducted, screening over 300 studies since August 2023. Inclusion criteria required (1) English-language studies, (2) reports on isolated chondral fragment fixation, and (3) a minimum of 6 months of follow-up. Eighteen studies met these criteria. Data on patient demographics, lesion characteristics, fixation methods, clinical outcomes, and functional scores were extracted.ResultsA total of 76 patients (mean age: 14.3 ± 3.7 years) were analyzed. Males comprised 80.3% of the cohort. The mean follow-up was 40.3 months (range: 7-171), and the mean chondral fragment size was 4.28 cm². The most common lesion locations were the lateral femoral condyle (34.2%), trochlea (32.2%), patella (25%), and medial femoral condyle (8.6%). Healing occurred in 96% of cases, and 86% of patients returned to sports at an average of 10.3 ± 6.1 months. Younger patients (≤14 years) had a significantly higher RTS rate (OR: 5.8; <i>P</i> = 0.0427). Functional scores (IKDC, KOOS, Marx, Tegner) demonstrated excellent postoperative outcomes.ConclusionDespite prior concerns regarding chondral-only fixation, this study demonstrates high healing rates and favorable functional outcomes. Direct fixation is a viable strategy, particularly in adolescents and young adults. Further prospective trials are needed to validate these findings.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251351781"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Toll-Like Receptor 3 Relieves Osteoarthritis by Suppression of Cartilage Degradation, Nuclear Factor Kappa B-Mediated Inflammation, and Activation of Autophagy.","authors":"Zhe Hou, Mou Wang, Shun Cao","doi":"10.1177/19476035251317713","DOIUrl":"10.1177/19476035251317713","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the regulatory role of Toll-like receptor 3 (TLR3) in osteoarthritis (OA) progression, particularly its impacts on cartilage degradation, NF-κB-mediated inflammation, and autophagy activation.</p><p><strong>Method: </strong>1. Model Constuction: OA mouse model generated via anterior cruciate ligament transection (ACLT); LPS-induced inflammatory injury in murine ATDC5 chondrocytes; Histomorphological analysis of cartilage tissue using H&E and Safranine O staining. 2. Molecular Detection: TLR3 expression assessed by Western blot; Cartilage degradation markers (MMP-13, ADAMTS) and NF-κB pathway proteins analyzed via Western blot; Pro-inflammatory cytokine levels (IL-1β, TNF-α) quantified via RT-qPCR and Western blot. 3. Functional Assays: Cell viability examined via CCK-8 assay.</p><p><strong>Results: </strong>1. TLR3 Upregulation: TLR3 was highly expressed in OA cartilage and LPS-treated chondrocytes. 2. Cartilage Protection: TLR3 inhibition reduced cartilage erosion and proteoglycan loss in ACLT mice (confirmed by H&E and Safranine O staining); Downregulation of cartilage degradation markers (MMP-13, ADAMTS-5) observed in TLR3-knockdown models. 3. Anti-inflammatory Effects: TLR3 knockdown suppressed NF-κB activation, reducing IL-1β and TNF-α levels. 4. Autophagy Activation: Enhanced LC3-II/LC3-I ratio and Beclin-1 expression indicated TLR3 inhibition promotes autophagy.</p><p><strong>Conclusion: </strong>TLR3 drives OA progression through dual mechanisms: 1. Pro-inflammatory Pathway: Activates NF-κB signaling to amplify cytokine release and cartilage matrix breakdown. 2. Autophagy Suppression: Inhibits autophagy-related proteins, impairing cellular homeostasis. Targeting TLR3 may represent a therapeutic strategy to balance inflammation and autophagy, potentially slowing OA progression in multi-joint involvement cases.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251317713"},"PeriodicalIF":2.7,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KDM3A Modulates Biological Processes in Osteoarthritis Cell Models Via the Wnt/β-Catenin Signaling Pathway.","authors":"Yang Fu, Shixiong Yi, Qifeng Peng, Heng Jiang, Jie Zhou","doi":"10.1177/19476035251344878","DOIUrl":"10.1177/19476035251344878","url":null,"abstract":"<p><p>BackgroundOsteoarthritis (OA) is a chronic disease that seriously affects human health. Although biomarkers are vital to the discovery and therapy of OA, current research on OA-specific biomarkers remains limited, indicating a need for further expansion of this field of study.MethodsIn this study, differential genes in OA patients and normal samples in Genomics Expression Omnibus (GEO) database were analyzed for signaling pathway enrichment. Then, Weighted Gene Co-expression Network Analysis (WGCNA) combined with Least Absolute Shrinkage and Selection Operator (LASSO) analysis was used to obtain key genes associated with OA diagnosis, including BCL6 co-repressor (BCOR), Coiled-Coil Domain Containing 59 (CCDC59), Jun Proto-Oncogene (JUN), Lysine Demethylase 3A (KDM3A), L3MBTL Histone Methyl-Lysine Binding Protein 4 (L3MBTL4) and Zinc Finger Protein 292 (ZNF292). Finally, the role of KDM3A in OA cell model was verified by constructing KDM3A overexpression and silencing cell lines.ResultsIt was found that overexpression of KDM3A significantly downregulated β-catenin expression compared with the oe-NC group, thus affecting a series of biological processes in the OA cell model, specifically, increasing antioxidant capacity, reducing levels of inflammatory factors, and inhibiting extracellular matrix degradation.ConclusionThis study not only provided six key target genes for OA but also revealed the important role of KDM3A in OA, providing a reference for gene targeted therapy for OA patients.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251344878"},"PeriodicalIF":2.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}