CARTILAGE最新文献_第4页

Transcriptional Analysis Reveals That the FHL1/JAK-STAT Pathway is Involved in Acute Cartilage Injury in Mice. 转录分析揭示 FHL1/JAK-STAT 通路参与了小鼠急性软骨损伤的过程

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-21 DOI: 10.1177/19476035251323601

Jian Lu, Zhenhua Shi, Lindan Geng, Dong Ren, Haowei Hou, Guowei Ren, Shuangquan Yao, Pengcheng Wang

{"title":"Transcriptional Analysis Reveals That the FHL1/JAK-STAT Pathway is Involved in Acute Cartilage Injury in Mice.","authors":"Jian Lu, Zhenhua Shi, Lindan Geng, Dong Ren, Haowei Hou, Guowei Ren, Shuangquan Yao, Pengcheng Wang","doi":"10.1177/19476035251323601","DOIUrl":"10.1177/19476035251323601","url":null,"abstract":"ObjectiveThis study aimed to identify genes and signaling pathways associated with acute cartilage injury using RNA sequencing (RNA-seq).MethodsKnee joint cartilage samples were collected from normal mice and 2 models of acute cartilage injury (non-invasive and groove models) within an 8-hour time limit. RNA-seq revealed differential gene expression between the injury models and controls, with subsequent validation using real-time quantitative polymerase chain reaction (RT-qPCR) for 9 representative genes.ResultsCompared to controls, the non-invasive model showed 36 differentially expressed genes (DEGs) (13 up-regulated, 23 down-regulated), with Gm14648 and Gm35438 showing the most significant upregulation and downregulation, respectively. The groove model exhibited 255 DEGs (13 up-regulated, 23 down-regulated), with Gm14648 and Gm35438 showing the (222 up-regulated, 33 down-regulated). Six overlapping genes were identified between the non-invasive and groove models, including up-regulated genes (Igfn1, Muc6, Hmox1) and down-regulated genes (Pthlh, Cyp1a1, Gm13490), validated by RT-qPCR. Gene ontology (GO) analysis highlighted involvement in environmental information processing and cartilage organ system function, while Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis implicated the JAK-STAT signaling pathway. RT-qPCR and immunohistochemistry confirmed downregulation of Fhl1 in the non-invasive model, supported by Western blotting of p-JAK2/t-JAK2 levels.ConclusionsThis study identifies DEGs (13 up-regulated, 23 down-regulated), with Gm14648 and Gm35438 showing the in acute cartilage injury, suggesting potential therapeutic targets. The role of Fhl1 in cartilage protection via the JAK-STAT pathway warrants further investigation in acute cartilage injury research.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251323601"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing Five Major Knee Osteoarthritis Cohort Studies: Similarities, Differences, and Unique Aspects of CHECK, OAI, FNIH, IMI-APPROACH, and MOST. 比较五项主要膝骨关节炎队列研究：CHECK、OAI、FNIH、IMI-APPROACH 和 MOST 的相似之处、不同之处和独特之处。

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-19 DOI: 10.1177/19476035251326276

Mariia Oliinyk, Simon C Mastbergen, Anne Karien C A Marijnissen, David T Felson, David J Hunter, Micheal C Nevitt, Harrie Weinans, Mylène P Jansen

{"title":"Comparing Five Major Knee Osteoarthritis Cohort Studies: Similarities, Differences, and Unique Aspects of CHECK, OAI, FNIH, IMI-APPROACH, and MOST.","authors":"Mariia Oliinyk, Simon C Mastbergen, Anne Karien C A Marijnissen, David T Felson, David J Hunter, Micheal C Nevitt, Harrie Weinans, Mylène P Jansen","doi":"10.1177/19476035251326276","DOIUrl":"10.1177/19476035251326276","url":null,"abstract":"ObjectiveTo analyze and synthesize the information available from five pivotal, large-scale, multicenter, observational studies (CHECK, OAI, FNIH Biomarkers Consortium, IMI-APPROACH, and MOST) focusing on knee osteoarthritis (OA), which can be used to elucidate disease progression, risk factors, and the effectiveness of potential interventions.DesignFor this narrative review, a comprehensive literature search and data extraction from official web pages and scientific databases were conducted to compare methodologies, in- and exclusion criteria, outcomes, and cohort characteristics across the studies. Thematic, comparative, and qualitative analyses were employed to identify trends, commonalities, and disparities among the findings.ResultsThe studies collectively enhanced understanding of the onset and progression of knee OA, and in several of the studies, hip OA, emphasizing the importance of both systemic and local risk factors. Advanced imaging and biomarkers are important components in all the cohorts, with the goal of aiding early diagnosis and tracking disease progression. All cohorts evaluated unique markers generally not available in the other cohorts, while other factors overlap, suggesting possibilities for combining or cross-validating between cohorts.ConclusionsThe collaborative efforts of major OA research significantly advance our understanding of knee OA. These studies highlight the importance of a multifaceted approach, integrating advanced imaging, biomarkers, and longitudinal data to tackle the complexities of OA. By synthesizing findings and addressing knowledge gaps such as heterogeneity of patients and used measurements, and use of novel pain measures, future research can develop more effective diagnostic tools and treatments, ultimately enhancing the quality of life for OA patients.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251326276"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and Cellular Predictors of Outcomes in Autologous Conditioned Plasma Therapy for Knee Osteoarthritis: A Prospective Cohort Study. 自体条件调节血浆疗法治疗膝骨关节炎疗效的临床和细胞预测因素：前瞻性队列研究

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-19 DOI: 10.1177/19476035251323376

Amr Selim, Tian Lan, Charlotte Hulme, Mike Williams, Jade Perry, Pete Gallacher, Paul Jermin, Karina Wright

{"title":"Clinical and Cellular Predictors of Outcomes in Autologous Conditioned Plasma Therapy for Knee Osteoarthritis: A Prospective Cohort Study.","authors":"Amr Selim, Tian Lan, Charlotte Hulme, Mike Williams, Jade Perry, Pete Gallacher, Paul Jermin, Karina Wright","doi":"10.1177/19476035251323376","DOIUrl":"10.1177/19476035251323376","url":null,"abstract":"IntroductionAutologous conditioned plasma (ACP) is a single-spin, leukocyte-poor platelet-rich plasma (PRP) that provides a plasma with a platelet concentration 2 to 3 times the blood platelet concentration. The objective of this study was to investigate the clinical effectiveness of ACP intra-articular injection in patients with knee osteoarthritis (OA) and to identify any demographic, disease-associated, or biological predictors of outcome.MethodsA prospective cohort study was conducted between 2022 and 2023 in a single high-volume tertiary center, including 42 patients (54 knees) who consented to be enrolled. Patients underwent a series of 3 injections of ACP at weekly intervals, prepared using the Arthrex ACP Double-Syringe System. Lysholm scores were collected at baseline, 3-months, and 6-months post-injection.ResultsForty patients (49 knees) completed the follow-up and were included in the final analysis. The mean age was 53.8 ± 10.16 years (range 35-76 years), and the median body mass index (BMI) was 29 (interquartile range [IQR]: 27-34). There were 22 females and 18 males. Treatment failure occurred in 12 out of 49 cases (24.49%). The mean platelet concentration in the ACP was 588.5 ± 183.2 × 106/ml, with a mean platelet fold increase of 2.14 ± 0.71 compared to the baseline. Multi-linear regression modeling showed that older age and higher mean platelet concentration were predictors of higher post-injection Lysholm scores, with beta coefficients of 0.34 and 0.28, respectively, and p values of 0.013 and 0.036, respectively.ConclusionAutologous conditioned plasma provided clinical benefits in this cohort study of knee OA patients for at least 6 months post-injection. Older age and a higher mean platelet concentration in the ACP were identified as predictors of a higher Lysholm score.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251323376"},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful Vitrification of Human Osteochondral Dowels and Intact Femoral Condyle. 成功玻璃化人骨软骨钉及完整股骨髁。

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-14 DOI: 10.1177/19476035251316715

Mohammadhamed Shahsavari, Shannon Clark, Janet A W Elliott, Nadr M Jomha

{"title":"Successful Vitrification of Human Osteochondral Dowels and Intact Femoral Condyle.","authors":"Mohammadhamed Shahsavari, Shannon Clark, Janet A W Elliott, Nadr M Jomha","doi":"10.1177/19476035251316715","DOIUrl":"10.1177/19476035251316715","url":null,"abstract":"ObjectiveCryopreservation via vitrification of articular cartilage (AC) will increase the availability of graft tissue for treating large joint defects. To advance this research area, we compared the effects of 2 cryopreservation protocols in which 10-mm diameter human osteochondral dowels were cooled and stored in liquid nitrogen vapor.DesignDowels collected from healthy human knee joints (n = 3 donors) of deceased donors were randomly assigned to Protocol 8 (430 min) or Protocol 2BWF (410 min). Post-warming chondrocyte viability was assessed and normalized to fresh controls.ResultsBoth protocols resulted in high chondrocyte viability after loading, vitrification, and rewarming (~80% of fresh control). Protocol 2BWF was subsequently used to vitrify and rewarm 3 human intact lateral femoral condyles. After rewarming, metabolic activity, normalized chondrocyte viability, histology, and matrix productivity were experimentally measured. Results documented ~82% of fresh chondrocyte viability post vitrification and rewarming, with similar results to the fresh control group on the other AC quality criteria.ConclusionThese results demonstrate that both Protocol 8 and Protocol 2BWF can preserve the quality of vitrified human AC in osteochondral dowels and human intact femoral condyles.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251316715"},"PeriodicalIF":2.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of the BMI (<30 kg/m² vs. ≥30 kg/m²) on the Surgical Outcome of Osteochondral Lesions of the Talus: Prospective Data from the German Cartilage Registry (KnorpelRegister DGOU). BMI （2 vs.≥30 kg/m2）对距骨骨软骨病变手术结果的影响：来自德国软骨登记（knnorpelregister DGOU）的前瞻性数据。

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-12 DOI: 10.1177/19476035241301294

Alena Richter, Anna Altemeier, Christoph Becher, Marco Güllmann, Christian Plaass, Sarah Ettinger

{"title":"Influence of the BMI (<30 kg/m2 vs. ≥30 kg/m2) on the Surgical Outcome of Osteochondral Lesions of the Talus: Prospective Data from the German Cartilage Registry (KnorpelRegister DGOU).","authors":"Alena Richter, Anna Altemeier, Christoph Becher, Marco Güllmann, Christian Plaass, Sarah Ettinger","doi":"10.1177/19476035241301294","DOIUrl":"10.1177/19476035241301294","url":null,"abstract":"ObjectiveAim of this study was to evaluate the 24 months follow-up data of the German Cartilage Registry (KnorpelRegister DGOU, GCR) regarding the influence of body mass index (BMI) on clinical outcomes after surgical osteochondral lesions of the talus (OCT) treatment.DesignA total of 303 patients met the inclusion criteria. Pre- and post-operative Foot and Ankle Outcome Score (FAOS) total scores, subscores, and ΔFAOS were analyzed for most frequent surgical techniques (bone marrow stimulation [BMS], matrix-augmented BMS, matrix-augmented BMS with additional bone grafting) in normal weight group (NW, BMI <30 kg/m2, n = 228) and obese weight group (OW, BMI ≥30 kg/m2, n = 75).ResultsBMI was significantly different in NW and OW (24.6 ± 2.97 [16.9-29.9] kg/m2 vs. 33.7 ± 4.0 [30.0-51.3] kg/m2, P < 0.001). Significant improvement from pre- to post-operative FAOS score and subscales was reached in both groups (NW: 64.2 ± 17.5 vs. 77.7 ± 17.8; OW: 52.3 ± 15.5 vs. 73.5 ± 20.2; P < 0.001) with higher pre- and post-operative scores in NW. No significant difference in ΔFAOS score was detected. Treatment technique did not influence the clinical outcome. OW showed an extended use of bone grafting due to greater defect depth. Age was significantly higher in OW compared to NW (35.7 ± 13.2 [18.0-69.0] years vs. 40.7 ± 13.1 [18.0-77.0] years, P = 0.005).ConclusionsPatients benefit from surgical cartilage therapy regardless of their BMI. OW showed significantly lower pre- and post-operative FAOS scores. In OW, additional bone grafting was required more frequently due to significantly deeper defects.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241301294"},"PeriodicalIF":2.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parallel Chondrogenesis and Osteogenesis Tissue Morphogenesis in Muscle Tissue via Combinations of TGF-β Supergene Family Members. TGF-β超基因家族成员在肌肉组织中的平行软骨形成和成骨组织形态发生

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2023-09-15 DOI: 10.1177/19476035231196224

Fei Xiong, Yan Chevalier, Roland M Klar

{"title":"Parallel Chondrogenesis and Osteogenesis Tissue Morphogenesis in Muscle Tissue via Combinations of TGF-β Supergene Family Members.","authors":"Fei Xiong, Yan Chevalier, Roland M Klar","doi":"10.1177/19476035231196224","DOIUrl":"10.1177/19476035231196224","url":null,"abstract":"Objective: This study aimed to decipher the temporal and spatial signaling code for clinical cartilage and bone regeneration. We investigated the effects of continuous equal dosages of a single, dual, or triplicate growth factor combination of bone morphogenetic protein (BMP)-2, transforming growth factor (TGF)-β3, and/or BMP-7 on muscle tissue over a culturing period. The hypothesis was that specific growth factor combinations at specific time points direct tissue transformation toward endochondral bone or cartilage formation.Design: The harvested muscle tissues from F-344 adult male rats were cultured in 96-well plates maintained in a specific medium and cultured at specific conditions. And the multidimensional and multi-time point analyses were performed at both the genetic and protein levels.Results: The results insinuate that the application of growth factor stimulates a chaotic tissue response that does not follow a chronological signaling cascade. Both osteogenic and chondrogenic genes showed upregulation after induction, a similar result was also observed in the semiquantitative analysis after immunohistochemical staining against different antigens.Conclusions: The study showed that multiple TGF-β superfamily proteins applied to tissue stimulate developmental tissue processes that do not follow current tissue formation rules. The findings contribute to the understanding of the chronological order of signals and expression patterns needed to achieve chondrogenesis, articular chondrogenesis, or osteogenesis, which is crucial for the development of treatments that can regrow bone and articular cartilage clinically.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"71-88"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10264117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-Short Echo Time-MRI T2* Mapping of Articular Cartilage Layers Is Associated with Histological Early Degeneration. 关节软骨层的超短回声时间MRI T2*标测与组织学早期退化有关。

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2023-10-16 DOI: 10.1177/19476035231205685

Rui Imamura, Atsushi Teramoto, Yasutaka Murahashi, Yohei Okada, Shinichiro Okimura, Yoshihiro Akatsuka, Kota Watanabe, Toshihiko Yamashita

{"title":"Ultra-Short Echo Time-MRI T2* Mapping of Articular Cartilage Layers Is Associated with Histological Early Degeneration.","authors":"Rui Imamura, Atsushi Teramoto, Yasutaka Murahashi, Yohei Okada, Shinichiro Okimura, Yoshihiro Akatsuka, Kota Watanabe, Toshihiko Yamashita","doi":"10.1177/19476035231205685","DOIUrl":"10.1177/19476035231205685","url":null,"abstract":"Objective: Ultra-short TE (UTE) sequences on MRI are a technique that improves the visualization of tissues with short T2 relaxation time, such as deep cartilage layers. In addition, T2* relaxation time calculated from the UTE has the potential to evaluate water molecules bound to the cartilage matrix. This study was performed to determine if there is an association between UTE-T2* relaxation time by cartilage layer and histological degeneration in knee osteoarthritis (OA).Design: Seven knees that had undergone total knee arthroplasty (TKA) were included in the study, and the lateral tibial cartilage, which had the least degeneration of the resected bones, was used as the sample. The T2* relaxation time of 4 patients with no abnormal findings on MRI was the reference relaxation time. Histological degeneration of TKA samples was assessed by the Mankin score and graded as the early OA group (≤3 points) and the advanced OA group (≥4 points). The association between T2* relaxation time and Mankin grade in each cartilage layer was compared. The effect of angiogenesis to the tidemark on T2* relaxation time was also compared.Results: T2* relaxation time of the cartilage layer was significantly longer in early OA than that in the control group. In the deep cartilage layer, the mean T2* relaxation time for angiogenesis (-) was 15.7 ms, whereas it was significantly shorter for angiogenesis (+) at 8.2 ms.Conclusions: The UTE-T2* relaxation time was associated with histological cartilage degeneration, suggesting a potential application in monitoring early cartilage degeneration.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"118-124"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Novel Rat Knee Osteoarthritis Model Induced by Medial Meniscus Extrusion. 内侧半月板挤压诱导大鼠膝骨性关节炎模型的建立。

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2023-10-14 DOI: 10.1177/19476035231205680

Daisuke Fukui, Daisuke Nishiyama, Manabu Yamanaka, Hidenobu Tamai, Naoko Nishio, Mamoru Kawakami, Hiroshi Yamada

{"title":"Development of a Novel Rat Knee Osteoarthritis Model Induced by Medial Meniscus Extrusion.","authors":"Daisuke Fukui, Daisuke Nishiyama, Manabu Yamanaka, Hidenobu Tamai, Naoko Nishio, Mamoru Kawakami, Hiroshi Yamada","doi":"10.1177/19476035231205680","DOIUrl":"10.1177/19476035231205680","url":null,"abstract":"Objective: The medial meniscus extrusion (MME) is associated with increased stress on the knee joint, which leads to cartilage degeneration. To evaluate the etiology of knee osteoarthritis, it is extremely important to create animal models of the disease that more closely resemble actual clinical conditions in terms of symptomatology, molecular biology, and histology. This study aimed to create a clinically relevant model of MME in rats.Design: Behavioral, molecular biological, and histological changes in the newly developed rat MME model were compared with those in sham and medial meniscus transection and medial collateral ligament transection (MMT) models to examine the characteristics of this model.Results: In the MME rat model, behavioral evaluation shows abnormalities in gait compared with the other 2 groups, and molecular biological evaluation of the infrapatellar synovia of rats shows that gene expression of inflammatory cytokines, matrix-degrading enzymes, and pain-related nerve growth factor was increased compared with the sham group. Furthermore, histological evaluation reveals that cartilage degeneration was the most severe in the MME group.Conclusions: The newly developed MME model reproduced the characteristic pathology of MME in clinical practice, such as severe pain, inflammation, and rapid progression of osteoarthritis. The MME model, which might more closely mimic human knee osteoarthritis (OA), could be a useful model for elucidating the pathophysiology and considering therapeutic management for knee OA.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"108-117"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41192225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL3 Regulates the m⁶A Modification of NEK7 to Inhibit the Formation of Osteoarthritis. METTL3调节NEK7的m6A修饰以抑制骨关节炎的形成。

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2023-09-19 DOI: 10.1177/19476035231200336

Xiaochuan Xiong, Hao Xiong, Jun Peng, Yingjie Liu, Yang Zong

{"title":"METTL3 Regulates the m6A Modification of NEK7 to Inhibit the Formation of Osteoarthritis.","authors":"Xiaochuan Xiong, Hao Xiong, Jun Peng, Yingjie Liu, Yang Zong","doi":"10.1177/19476035231200336","DOIUrl":"10.1177/19476035231200336","url":null,"abstract":"Objective: Osteoarthritis (OA) is a common degenerative joint disease. The occurrence of OA slowly destroys the soft tissue structure of the patient's joint. Severe cases could lead to disability. Current studies had shown that inhibition of chondrocytes pyroptosis could slow down the progression of OA. Our work aimed to explore the specific mechanisms and ways of regulating this process.Design: In this work, the level of N6-methyladenosine (m6A) in clinical tissues was detected by ribonucleic acid (RNA) m6A dot blot. qRT-PCR (quantitative real-time polymerase chain reaction) was used to detect the messenger RNA (mRNA) expression level of m6A modified enzyme in clinical tissues. MTT (3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromid) and flow cytometry were used to detect the effect of sh-METTL3 (methyltransferase like 3) and NIMA-related kinase 7 (NEK7) transfection on chondrocytes pyroptosis in OA. Western blot was used to detect the protein expression levels of pyroptosis-related proteins. ELISA (enzyme-linked immunosorbent assay) was used to measure the protein concentration of inflammatory cytokines. The SRAMP online database was used to predict the m6A site of NEK7. HE staining was used to assess the progression of OA in mice.Results: The level of m6A in clinical samples of OA patients was higher, and METTL3 was significantly higher expressed in clinical samples of OA patients. We provided evidence that low expression of METTL3 inhibited chondrocytes pyroptosis. In addition, Rescue experiments and in vivo experiments had shown that METTL3 in combination with NEK7 inhibited the progression of OA by promoting chondrocytes pyroptosis.Conclusions: METTL3 regulates m6A modification of NEK7 and inhibits OA progression.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"89-99"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41100820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arthroscopic Implantation of a Cell-Free Bilayer Scaffold for the Treatment of Knee Chondral Lesions: A 2-Year Prospective Study. 关节镜下植入无细胞双层支架治疗膝关节软骨损伤：一项为期两年的前瞻性研究

IF 2.7 4区医学

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2024-03-19 DOI: 10.1177/19476035241232061

Rimtautas Gudas, Mantas Staškūnas, Justinas Mačiulaitis, Emilė Gudaitė, Ieva Aleknaite-Dambrauskiene

{"title":"Arthroscopic Implantation of a Cell-Free Bilayer Scaffold for the Treatment of Knee Chondral Lesions: A 2-Year Prospective Study.","authors":"Rimtautas Gudas, Mantas Staškūnas, Justinas Mačiulaitis, Emilė Gudaitė, Ieva Aleknaite-Dambrauskiene","doi":"10.1177/19476035241232061","DOIUrl":"10.1177/19476035241232061","url":null,"abstract":"Objective: The main objective of this study is to assess the safety and clinical efficacy of a cell-free bilayer scaffold (MaioRegen Chondro+ by Fin-Ceramica) in patients affected by chondral knee lesions of different origin and localization.Design: Thirty-one patients with focal chondral lesions of the knee were arthroscopically treated with MaioRegen Chondro+. All patients were prospectively evaluated for a minimum of 2 years using the International Knee Documentation Committee (IKDC) Questionnaire and the Tegner Activity Scale. Cartilage repair was assessed based on the Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) 2.0 score at 12 months. Follow-up at 36 months was available for 25 out of 31 patients.Results: From baseline to 6-, 12-, and 24-month follow-up, IKDC score significantly improved by 19.5 ± 7.27 (95% confidence interval [CI]: 16.9-22.2, P < 0.001), 30.8 ± 7.63 (95% CI: 28.0-33.6, P < 0.001), and 36.2 ± 8.00 points (95% CI: 33.3-39.2, P < 0.001), respectively. Tegner scores documented a substantial clinical improvement as early as 12 months after surgery (change of -0.6 ± 0.62; 95% CI: -0.8 to -0.4, P < 0.001), reaching the preinjury values. There was a statistically significant increase in the MOCART scores (P < 0.001). Comparable results were observed regardless of preintervention demographic characteristics, lesion site or etiology, or the number of treated sites. Notably, the significant clinical benefit was maintained in a subset of patients who reached 3-year follow-up. No adverse events were reported in the entire analyzed population.Conclusion: MaioRegen Chondro+ is a safe and effective device for the treatment of knee chondral lesions, enabling a significant clinical improvement for at least 2 years.","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"5-16"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140157585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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