CARTILAGE最新文献

{"title":"What are These Cysts Doing in My Graft? A Meta-Analysis on Cystic Occurrence After Autografting and Allografting for Osteochondral Lesions of the Talus.","authors":"Jari Dahmen, Julian J Hollander, James J Butler, Kaj S Emanuel, Quinten G H Rikken, Sjoerd A S Stufkens, John G Kennedy, Gino M M J Kerkhoffs","doi":"10.1177/19476035251333374","DOIUrl":"https://doi.org/10.1177/19476035251333374","url":null,"abstract":"<p><p>BackgroundThe exact incidence of cyst formation after graft transplantation for osteochondral lesions is unknown. The primary purpose was to assess and compare cystic occurrence after autografting, allografting, and osteoperiosteal grafting for osteochondral lesions of the talus. Our secondary aim was to assess the correlation of clinical outcomes with the presence of postoperative cysts.MethodsA literature search was performed up to October 2023 through PubMed, Embase (Ovid), and Cochrane Library. The primary outcome was the postoperative cystic occurrence rate. A random-effects model with moderator analysis was used to calculate differences in occurrence rates between treatment groups. The relationship between the presence of cysts and clinical outcomes was described.ResultsThirteen studies were included with 382 ankles. The average radiological follow-up at which the presence of cystic occurrence was assessed ranged from 12 to 84 months. The rates of cystic occurrence for the osteochondral autograft transplantation group, the allograft transplantation group, and the osteoperiosteal transplantation group were 42% (95% confidence interval [CI] = 24-61), 58% (95% CI = 40-74), and 34% (95% CI = 12-67), respectively, without any significant differences noted. No relationship between the presence of cysts and clinical outcomes was found.ConclusionPostoperative cystic occurrence is common after osteochondral autograft transplantation (42%), allograft transplantation (58%), and osteoperiosteal transplantation (34%) in osteochondral lesions of the talus-without significant intertreatment differences. The postoperative presence of cysts was not correlated with clinical outcomes. Future research should assess whether the postoperative presence of cysts correlates with (clinical) outcomes at longer follow-up.Level of Evidence:Level IV, systematic review and meta-analysis.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251333374"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimal Clinically Important Difference in Patients with Knee Cartilage Lesions Treated with a Cell-Free Scaffold Implantation.","authors":"Luca De Marziani, Angelo Boffa, Marco Franceschini, Luca Andriolo, Alessandro Di Martino, Stefano Zaffagnini, Giuseppe Filardo","doi":"10.1177/19476035251322730","DOIUrl":"https://doi.org/10.1177/19476035251322730","url":null,"abstract":"<p><p>ObjectiveThe aim of this study was to establish the minimal clinically important difference (MCID) thresholds for the International Knee Documentation Committee (IKDC) subjective and Visual Analogue Scale (VAS) pain scores in patients affected by knee chondral and osteochondral lesions treated with cell-free scaffold implantation.DesignFor the MCID definition, 186 patients who underwent an osteochondral scaffold implantation were included. Patients were evaluated through the IKDC subjective and VAS pain scores at baseline, 12 and 24 months. The MCID was calculated using a distribution-based method for both IKDC subjective and VAS pain scores at 12 and 24 months, as well as with an anchor-based method.ResultsThe MCID values were 10.1 and 1.5 for the IKDC subjective and VAS pain scores, respectively, both at 12 and 24 months of follow-up. The rate of patients who achieved the MCID was 83% at 12 months and 88% at 24 months. The anchor-based method led to higher MCID values. Factors identified to increase the probability to reach the MCID were younger age (<i>P</i> = 0.042), male sex (<i>P</i> = 0.042), and lateral femoral condyle lesions (<i>P</i> = 0.002), while patellar lesions were less likely to reach the MCID (<i>P</i> = 0.009).ConclusionsThis study defined the MCID values for the IKDC subjective and VAS pain scores after treatment with a cell-free biomimetic scaffold, with 88% of the patients achieving clinically relevant results at 2 years. Younger patients, males and lateral femoral condyle lesions were more likely to reach the MCID. However, the identified thresholds can be influenced by the method chosen, which warrants caution when interpreting study results.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251322730"},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of MACI (Autologous Cultured Chondrocytes on Porcine Collagen Membrane) in the United States: Expanded Experience over 5,000 Cases.","authors":"Eric M Milliron, Parker A Cavendish, James Carey, Tyler Barker, David C Flanigan","doi":"10.1177/19476035251319404","DOIUrl":"10.1177/19476035251319404","url":null,"abstract":"<p><p>ObjectiveTo determine whether there are differences in MACI (matrix-induced autologous chondrocyte implantation) treatment in the United States, by comparing cartilage defects and patient characteristics between the initial 1,000 patients treated with the next 5,000MethodsFollowing initial analysis of the first 1,000 consecutive patients treated with MACI, data were collected and analyzed for the subsequent 5,000. Patients were identified by MACI lot number and surgery date. Adverse events were summarized with descriptive statistics. Group differences were assessed with <i>t</i>-tests and chi-square, with significance set at <i>P</i> < 0.05.ResultsFive thousand adults (5,198 knees) were implanted with MACI by 1,130 surgeons. Patient sex (male 49.2%) was evenly split, and the mean age was 33.6 years. Most patients had a single cartilage defect treated, and the mean defect size was 4.4 cm². The patella was the most treated surface (38.4%), followed by the medial femoral condyle (25.7%). Most patients (85.5%) had concomitant surgical procedures at the time of cartilage biopsy procurement. There were statistically significant differences in the number of patella (<i>P</i> < 0.001), medial femoral condyle (<i>P</i> < 0.001), and \"not specified\" (<i>P</i> = 0.008) between groups. Mean defect size and mean total defect size were both larger (<i>P</i> < 0.001 and <i>P</i> = 0.009, respectively) in the subsequent 5,000 patients.ConclusionThe utilization of MACI has remained consistent. Patient demographics and concomitant surgical procedures between the first 1,000 MACI patients and subsequent 5,000 MACI patients were comparable. Patellofemoral defects were the most treated in both subsets, and an overall low rate of adverse events was observed.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251319404"},"PeriodicalIF":2.7,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Organ Magnetic Resonance Imaging Score (WORMS) of the Knee in Professional Soccer Players.","authors":"Goetz Hannes Welsch, Marc Regier, Karl-Heinz Frosch, Milena L Pachowsky, Frank Oliver Henes, Gerhard Adam, Kai-Jonathan Maas, Malte Lennart Warncke","doi":"10.1177/19476035251329571","DOIUrl":"10.1177/19476035251329571","url":null,"abstract":"<p><p>ObjectiveThe goal of our study was to assess the prevalence of osteoarthritis in the knee joint of active male professional soccer players by means of the semi-quantitative Whole-Organ Magnetic Resonance Imaging Score (WORMS).DesignMagnetic resonance imagings (MRIs) of both knees were performed on 85 male professional soccer players during their \"medicals\" (age = 24 ± 4 years). All baseline data (age, playing position) were obtained. Based on the WORMS, the status of the cartilage and bone in the medial and lateral femoro-tibial joint (MFTJ and LFTJ), as well as the patellofemoral joint was assessed. Menisci and ligaments were evaluated separately. The final score was the sum of all regional scores.ResultsThe mean WORMS of the 170 knee joints was 13.3 ± 13.5 points (range = 0-111, achievable scores: 0-290). Cartilage changes were the most common pathologies, observed in 141 of the 170 knee joints. Structural cartilage lesions (WORMS ≥ 2) were observed in 54% of the studied knees. Pathologies of the medial meniscus were associated with cartilage damage of the corresponding MFTJ (r = 0.424, <i>P</i> < 0.0001). The same effect was observed for the lateral meniscus and the corresponding LFTJ (r = 0.553, <i>P</i> < 0.0001). However, lateral meniscal lesions could be correlated with more other joint pathologies compared to medial meniscal lesions. Total WORMS correlated significantly with increasing age (r = 0.386, <i>P</i> = 0.001).ConclusionThe provided data show the high incidence of knee joint damage in professional football players. In particular, the lateral meniscus appears to play a critical role.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251329571"},"PeriodicalIF":2.7,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EUROVISCO Good Medical Practice Recommendations for the Use of Viscosupplementation with Hyaluronic Acid in the Management of Knee Osteoarthritis.","authors":"Jordi Monfort, Yves Henrotin, Xavier Chevalier, Alberto Migliore, Dominique Baron, Jörg Jerosch, Pascal Richette, Hervé Bard, Mats Brittberg, Demirhan Diraçoglù, Raghu Raman, Thierry Conrozier","doi":"10.1177/19476035241286578","DOIUrl":"https://doi.org/10.1177/19476035241286578","url":null,"abstract":"<p><strong>Objective: </strong>Viscosupplementation (VS) by intra-articular injections of hyaluronic acid (HA) is a commonly used treatment of knee osteoarthritis. However, there are no clear recommendations for its use in clinical situations not usually addressed in therapeutic guidelines.</p><p><strong>Design: </strong>Members of the European Viscosupplementation Consensus Group (EUROVISCO), made up of rheumatologists, orthopedic surgeons, and rehabilitation physicians from seven European countries, were asked to make a therapeutic decision on 30 clinical scenarios based on their clinical experience and data from the literature, using the Delphi method. For each scenario, the strength of agreement and the level of consensus were calculated by the chairman of the group.</p><p><strong>Results: </strong>The expert panel reached consensus on 18 of the 30 clinical scenarios proposed. According to the experts, the factors that most influence the decision to use VS with HA to treat knee osteoarthritis are the nature and severity of symptoms, the presence of comorbidity, the absence of therapeutic alternatives, and the patient's refusal to undergo knee arthroplasty. Obesity and an advanced radiological degree of osteoarthritis have been identified as the two main factors for poor response to VS with HA and should be considered before any decision is made to viscosupplement.</p><p><strong>Conclusion: </strong>This set of recommendations, based on common clinical scenarios, is intended to help practitioners make decisions about HA VS in patients with osteoarthritis of the knee.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241286578"},"PeriodicalIF":2.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercial Insurance Payer Coverage Criteria for Meniscal Allograft Transplantation Poorly Reflect Modern Indications for the Procedure.","authors":"Jacob L Kotlier, Amir Fathi, Meng-Yung Ong, Cailan L Feingold, Eric H Lin, Ryan D Freshman, Ioanna K Bolia, Frank A Petrigliano, Joseph N Liu","doi":"10.1177/19476035251329223","DOIUrl":"10.1177/19476035251329223","url":null,"abstract":"<p><p>ObjectiveTo investigate whether insurance coverage criteria for meniscal allograft transplantation (MAT) are sufficiently supported in the policy documentation and whether these criteria represent current research and expert consensus on indications for the procedure.DesignThe top 11 United States (US)-based national commercial health insurance payers for MAT were identified. A Google search was performed to identify payer coverage policies. Cited references within policy documents were classified by type of reference and reviewed for level of evidence (LOE). Specific coverage criteria for each individual payer were then extracted and compared to assess for similarities among commercial payers. Finally, all references cited were examined to determine whether they supported the coverage criteria stated by policies for each specific payer.ResultsSeven of the 11 payers had accessible coverage policies. This study found that the majority of cited references were primary journal articles (20, 57.1%) and that the vast majority of references cited (27, 77.1%) were level IV evidence. Of the seven payers, only two (Cigna = 8, HCSC = 19) cited more than six sources. There was a high degree of homogeneity in coverage criteria among payers. The sources cited did not consistently support specific payer coverage criteria. Payer criteria also tended to be arbitrary and poorly supported by current evidence on MAT.ConclusionThis study demonstrates that insurance coverage policies for MAT frequently use outdated references or cite references inappropriately. In addition, these policies fail to reflect current research and consensus on indications for the procedure.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251329223"},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mean Cartilage Attenuation Measured <i>In Situ</i> in an Osteoarthritis Mouse Model Using Contrast-Enhanced Micro-Computed Tomography is Indicative of Cartilage Degeneration.","authors":"Jemima E Schadow, Emma C Boersma, Rachel A Davey, Kathryn S Stok","doi":"10.1177/19476035251323373","DOIUrl":"10.1177/19476035251323373","url":null,"abstract":"<p><p>ObjectiveTo develop and validate an <i>in vivo</i> image acquisition protocol for assessment of cartilage degeneration in an osteoarthritis (OA) mouse model using contrast-enhanced micro-computed tomography (CECT).DesignThree-, four-, seven-, and 10-month-old male STR/ort OA (<i>n</i> = 8) and healthy control CBA/1 (<i>n</i> = 8) mice were imaged using synchrotron-radiation micro-computed tomography. Mean attenuation of cartilage and mean attenuation difference between cartilage and contrast agent (mean Δ attenuation), to control for flux, were calculated and correlated to previously published cartilage parameters measured in the same mice. In a second experiment, C57Bl/6 mice underwent destabilization of medial meniscus (DMM) surgery or sham surgery. At 0 (<i>n</i> = 8 DMM), and 4 weeks (<i>n</i> = 10 DMM, <i>n</i> = 6 sham) post-surgery, mice were imaged using CECT. Mean attenuation and Osteoarthritis Research Society International (OARSI) score of cartilage were calculated, and mean attenuation and OARSI score were correlated.ResultsMean Δ attenuation of medial cartilage was lower in STR/ort OA mice compared to healthy control CBA/1 at 3-, 4-, 7-, and 10-months of age. Medial mean Δ attenuation was positively correlated to cartilage volume and thickness and negatively correlated with surface-to-volume ratio and Collins score. The mean attenuation and OARSI score of cartilage in DMM OA mice was higher than in sham controls in the medial tibia. Mean attenuation was positively correlated with OARSI score in the medial tibia.ConclusionsAn <i>in vivo</i> CECT imaging method was developed and validated. Mean attenuation is sensitive to cartilage degeneration in OA mouse models when imaged against a contrast agent as a background and using <i>in vivo</i> compatible image acquisition parameters.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251323373"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensionally Measured TT-TG Distance Remains After Medial Open-Wedge High Tibial Osteotomy and Correlates With Internal Rotation of Distal Tibial Segment Below the Osteotomy Site.","authors":"Se-Han Jung, Min Jung, Kwangho Chung, Hyun-Soo Moon, Sungjun Kim, So-Heun Lee, Chong-Hyuk Choi, Sung-Hwan Kim","doi":"10.1177/19476035251327025","DOIUrl":"10.1177/19476035251327025","url":null,"abstract":"<p><p>ObjectiveTo evaluate perioperative changes in anatomical parameters related to patellofemoral biomechanics, as well as cartilage repair outcomes following additional arthroscopic marrow stimulation for full-thickness trochlear cartilage defects (TCDs) during medial open-wedge high tibial osteotomy (MOWHTO).DesignA total of 38 patients (38 knees) who underwent arthroscopic marrow stimulation (microfracture or microdrilling) for TCDs combined with MOWHTO were retrospectively reviewed. The mean follow-up period was 27.0 months. Three-dimensional measurements of parameters associated with patellar biomechanics were performed. Magnetic resonance imaging and second-look arthroscopy were used to assess repaired cartilage, using Magnetic Resonance Observation of Cartilage Repaired Tissue (MOCART) knee score and International Cartilage Repair Society Cartilage repair assessment (ICRS CRA).ResultsIn 3-dimensional measurements, the tibial tubercle-trochlear groove (TT-TG) distance was maintained (mean difference: -0.6 mm, <i>P</i> = 0.227), whereas the modified Q-angle significantly decreased (mean difference: -1.9°, <i>P</i> = 0.002). The distal femur-distal tibial rotation (DF-DTR) decreased, indicating internal rotation of the distal tibial segment (mean difference: -5.3°, <i>P</i> < 0.001). TT-TG distance was associated with DF-DTR (<i>r</i> = 0.583, <i>P</i> = 0.001). Arthroscopic marrow stimulation for TCDs showed favorable cartilage repaired tissue, especially for the microdrilling group (median MOCART score, 70; ICRS CRA grade 1-2, 82.8%).ConclusionThe 3-dimensionally measured TT-TG distance was maintained after MOWHTO and was associated with internal rotation of the distal tibial segment below the osteotomy site. Arthroscopic marrow stimulation for TCDs in patients undergoing MOWHTO achieved favorable cartilage repaired tissue in short term.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251327025"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Responses of Articular Chondrocytes from Diabetic and Non-Diabetic Rats to Glucose Conditions and Inflammatory Stimuli: Influence of a Vitamin K2 Enriched Diet.","authors":"Vivienne Rüdig, Tobias Braun, Nils Fleischmann, Alexander Reinhardt, Julius Wehrmann, Clemens Gögele, Maria Kokozidou, Christian Werner, Ingo Mrosewski, Gundula Schulze-Tanzil","doi":"10.1177/19476035251317091","DOIUrl":"10.1177/19476035251317091","url":null,"abstract":"<p><p>ObjectiveMeanwhile, the association between osteoarthritis (OA) and type 2 diabetes mellitus (T2DM) is well known. However, it remains unclear whether vitamin K2 (vit.K2) could exert chondroprotective effects. Hence, this study investigates the interrelation between OA and T2DM under the influence of vit.K2 in chondrocytes.MethodsUsing an <i>in vitro</i> OA/T2DM model, articular chondrocytes were harvested from adult male Zucker diabetic fatty (ZDF) Leptfa/Crl rats, categorized as non-diabetic (heterozygous: fa/+) or diabetic (homozygous: fa/fa). Based on vit.K2 supplementation of the rats, four groups arose: control without or with vit.K2 and diabetic without or with vit.K2 supplementation. Inflammatory conditions simulating OA were induced by exposing chondrocytes to tumor necrosis factor alpha (TNFα) and C5a. Chondrocyte response was analyzed using proliferation, metabolic and wound healing assays, immunolabeling, as well as gene expression analyses.ResultsThe proliferation of chondrocytes from control rats with vit.K2 supplementation was significantly higher than those without vit.K2 feeding, under both normoglycemic (NG) and hyperglycemic (HG) conditions. The wound closure ability of chondrocytes was significantly higher in the non-diabetic compared with the diabetic chondrocyte donor group. TNFα and C5a exerted catabolic effects under HG conditions by significantly inducing <i>Tnf</i><i>α</i> gene activity in chondrocytes of control rats without vit.K2 supplementation and a significant reduction of collagen type 2 gene expression in those cells of control rats with vit.K2 supplementation.ConclusionsThe response of chondrocytes derived from non-diabetic and diabetic donors differed. The vit.K2 supply of chondrocyte donor rats exerted anabolic effects on chondrocytes.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251317091"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional Analysis Reveals That the FHL1/JAK-STAT Pathway is Involved in Acute Cartilage Injury in Mice.","authors":"Jian Lu, Zhenhua Shi, Lindan Geng, Dong Ren, Haowei Hou, Guowei Ren, Shuangquan Yao, Pengcheng Wang","doi":"10.1177/19476035251323601","DOIUrl":"10.1177/19476035251323601","url":null,"abstract":"<p><p>ObjectiveThis study aimed to identify genes and signaling pathways associated with acute cartilage injury using RNA sequencing (RNA-seq).MethodsKnee joint cartilage samples were collected from normal mice and 2 models of acute cartilage injury (non-invasive and groove models) within an 8-hour time limit. RNA-seq revealed differential gene expression between the injury models and controls, with subsequent validation using real-time quantitative polymerase chain reaction (RT-qPCR) for 9 representative genes.ResultsCompared to controls, the non-invasive model showed 36 differentially expressed genes (DEGs) (13 up-regulated, 23 down-regulated), with <i>Gm14648</i> and <i>Gm35438</i> showing the most significant upregulation and downregulation, respectively. The groove model exhibited 255 DEGs (13 up-regulated, 23 down-regulated), with <i>Gm14648</i> and <i>Gm35438</i> showing the (222 up-regulated, 33 down-regulated). Six overlapping genes were identified between the non-invasive and groove models, including up-regulated genes (<i>Igfn1, Muc6, Hmox1</i>) and down-regulated genes (<i>Pthlh, Cyp1a1, Gm13490</i>), validated by RT-qPCR. Gene ontology (GO) analysis highlighted involvement in environmental information processing and cartilage organ system function, while Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis implicated the JAK-STAT signaling pathway. RT-qPCR and immunohistochemistry confirmed downregulation of <i>Fhl1</i> in the non-invasive model, supported by Western blotting of p-JAK2/t-JAK2 levels.ConclusionsThis study identifies DEGs (13 up-regulated, 23 down-regulated), with <i>Gm14648</i> and <i>Gm35438</i> showing the in acute cartilage injury, suggesting potential therapeutic targets. The role of <i>Fhl1</i> in cartilage protection via the JAK-STAT pathway warrants further investigation in acute cartilage injury research.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251323601"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}