CARTILAGE最新文献

{"title":"Texture Analysis of Cartilage Repair Tissue Maturation: Comparison of Two Cartilage Repair Methods and Correlation with MOCART 2.0.","authors":"Veronika Janacova, Pavol Szomolanyi, Diana Sitarcikova, Alexandra Kirner, Siegfried Trattnig, Vladimir Juras","doi":"10.1177/19476035241313047","DOIUrl":"10.1177/19476035241313047","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to assess the maturation of matrix-associated autologous chondrocyte transplantation (MACT) grafts up to 2 years after the surgery using gray-level co-occurrence matrix (GLCM) texture analysis of quantitative T₂ maps, compare the results with the microfracturing technique (MFX) control group, and relate these results to the morphological MOCART 2.0 score.</p><p><strong>Design: </strong>A subcohort of 37 patients from prospective, multi-center study underwent examination on a 3T MR scanner, including a T₂ mapping sequence at 3, 12, and 24 months after surgery. Changes between the time-points in the mean T₂ values and 20 GLCM features extracted from T₂ maps were assessed in repair tissue, tissue adjacent to the repair site, and the reference cartilage for both procedures.</p><p><strong>Results: </strong>Significant correlations were found between the MOCART 2.0 and GLCM features for both surgical procedures. There were no significant differences between MACT and MFX. We identified significant intra-group changes in T₂ and autocorrelation (3M-12M: <i>P</i> = 0.002; 3M-24M: <i>P</i> = 0.004), dissimilarity (3M-24M: <i>P</i> = 0.01), homogeneity (3M-24M: <i>P</i> = 0.013), and correlation (3M-24M: <i>P</i> = 0.036), sum average (3M-12M: <i>P</i> = 0.001; 3M-24M: <i>P</i> = 0.002), and information measure (3M-24M: <i>P</i> < 0.001) in the MACT repair tissue. MACT models revealed differences in GLCM between all combinations of ROI types at almost all time-points. In the case of MFX, the significant differences were mainly between repair and reference tissue at 12 months.</p><p><strong>Conclusion: </strong>Texture analysis provides a useful extension to T₂ mapping. Texture features are correlated to the morphological outcome and reveal differences in the process of maturation between MACT and MFX.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241313047"},"PeriodicalIF":2.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hinge Position Dominance Over Osteotomy Inclination in Medial Open-Wedge High Tibial Osteotomy: A Key Factor in Posterior Tibial Slope Changes.","authors":"Eva A Bax, H Chien Nguyen, Nienke van Egmond, Cornelis H Slump, Moyo C Kruyt, Roel J H Custers, Edsko E G Hekman","doi":"10.1177/19476035241311233","DOIUrl":"10.1177/19476035241311233","url":null,"abstract":"<p><strong>Objective: </strong>A medial open-wedge high tibial osteotomy (MOWHTO) may increase the posterior tibial slope (PTS). The purpose of this study was to determine the effect of the osteotomy inclination angle (in the sagittal plane) in combination with different hinge positions (in the transverse plane) on the change in PTS due to a MOWHTO.</p><p><strong>Methods: </strong>We developed a mathematical approach to determine the effect of the osteotomy inclination angle combined with different hinge positions. The change in PTS was determined for different osteotomy inclination angles, hinge positions, and intended wedge angles. Anterior-inclined, parallel, and posterior-inclined osteotomy inclination angles were simulated. Hinge positions varied between 5° anterolateral and -45° posterolateral. The wedge angles were 5°, 10°, and 15°. Moreover, 2 in silico osteotomies were performed to verify the results of the mathematical model.</p><p><strong>Results: </strong>The PTS was maintained when the osteotomy cut was performed parallel to the tibial plateau with a lateral hinge position. The PTS changed when the osteotomy was not aligned in the sagittal plane, ranging between 0.0° and 0.6°. Different hinge positions, however, had a large effect on postoperative PTS change, ranging between 0.1° and 10.7°.</p><p><strong>Conclusions: </strong>Our mathematical approach showed that the hinge position has a strong effect on the PTS. The sagittal osteotomy inclination angle had little effect on the PTS. An inclination angle parallel to the medial tibial plateau combined with a lateral hinge position does not change the PTS.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241311233"},"PeriodicalIF":2.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-Articular Administration of Ganglioside Sugars Protects Cartilage from Progressive Degeneration in an Instability OA Rabbit Model.","authors":"Masanari Hamasaki, Tomohiro Onodera, Junichi Furukawa, Masahiro Todoh, Yuma Sakai, Taku Ebata, Mohamad Alaa Terkawi, Kentaro Homan, Norimasa Iwasaki","doi":"10.1177/19476035241311542","DOIUrl":"10.1177/19476035241311542","url":null,"abstract":"<p><strong>Objective: </strong>Osteoarthritis (OA) is a degenerative joint disease that has no cure, and current therapies are intended to minimize pain. There is, therefore, a need for effective pharmacologic agents that reverse or slow the progression of joint damage. We report herein on an investigation of the effects of intra-articular injections of ganglioside sugars on the progression of OA in an experimental rabbit model.</p><p><strong>Design: </strong>Knee OA was induced Japanese in White rabbits by anterior cruciate ligament transection (ACLT). Ganglioside sugars at concentrations of 0.1, 0.3, and 0.9 mg/ml were then intra-articularly injected as a possible treatment for OA. Controls received intra-articular injections of saline. Knees were assessed macroscopically, histologically, and mechanically at 13 weeks after ACLT induction.</p><p><strong>Results: </strong>Macroscopically, knees of the groups that received ganglioside sugars at concentrations of 0.3 and 0.9 mg/ml exhibited milder cartilage degradation compared to the controls. Consistent with these results, histological scores for these knees were significantly higher than the corresponding values for the control knees. Lectin histochemistry staining revealed that the treatment with ganglioside sugars at concentrations of 0.3 and 0.9 mg/ml was associated with a remarkable increase in the levels of GalNAc-positive chondrocytes in cartilage. Coefficient of friction testing also demonstrated that cartilages treated with ganglioside sugars had a lower coefficient of frictions than the values for the control group.</p><p><strong>Conclusions: </strong>Intra-articular injections of ganglioside sugars prevented cartilage degeneration in an OA-instability model. These results highlight the promising therapeutic potential for using ganglioside sugars in the treatment of progressive OA.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241311542"},"PeriodicalIF":2.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associated Anatomic Abnormalities in Patients Undergoing Rotational Tibial Osteotomies for Patellofemoral Pathology and Implications for the Level of Correction.","authors":"Mohammad Haikal, Nishant Singh, Renjit Issac, Gwithyen Silk, Martyn Snow","doi":"10.1177/19476035241311548","DOIUrl":"10.1177/19476035241311548","url":null,"abstract":"<p><strong>Purpose: </strong>Tibial rotational deformity is a known risk factor for patellofemoral joint (PFJ) disorders. However, it is commonly associated with other abnormalities which affect the PFJ. The purpose of this study was to describe the prevalence of associated factors known to affect PFJ in patients undergoing rotational tibial osteotomy and their implication for the correction level.</p><p><strong>Methods: </strong>All patients with PFJ disorder, who underwent rotational tibial osteotomy between July 2009 and February 2020, were included. Patients were excluded if there were no preoperative computed tomography (CT)/magnetic resonance imaging (MRI). Scans were analyzed by 2 observers. Parameters of interest were femoral version, tibial torsion, trochlear dysplasia, lateral trochlear inclination (LTI), tibial tuberosity-trochlear groove (TT-TG) distance, Insall-Salvati (IS), and Caton Deschamps Index (CDI).</p><p><strong>Results: </strong>The search resulted in 80 knees, which had a mean femoral version of 21.0 ± 11.7, tibial torsion of 45.9 ± 9.1, TT-TG of 18.3 ± 5.5, and LTI of 11.4 ± 14.7. In total, 42.5% had TT-TG value of ≥ 20 mm. Patella alta/baja was found in 54% based on CDI or IS >1.2 and <0.8. High-grade trochlear dysplasia was found in 25%. In total, 29% had a tibial torsion abnormality but normal TT-TG and patella height. In total, 18% had abnormal TT-TG but normal patellar height.</p><p><strong>Conclusion: </strong>Based on the associated abnormalities of patella height and tubercle lateralization, 18% of the population were suitable for supratubercle osteotomy, and 29% of patients were suitable for diaphyseal or distal tibial osteotomy. A tibial tubercle osteotomy was required in 54% of patients, making a through-tubercle approach the most appropriate option for most patients.</p><p><strong>Level of evidence: </strong>Radiological cross-sectional study.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241311548"},"PeriodicalIF":2.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11724394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk of Chondral Fracture During Implantation of Distal Femur Osteochondral Autograft Plugs: A Human Cadaveric Comparison of Four Different Donor Regions.","authors":"Patrick A Massey, Daniel Hayward, Christian Bonner, Wayne Scalisi, Ryan Taylor, Elise Vincent, Robert W Rutz, Giovanni F Solitro","doi":"10.1177/19476035241308535","DOIUrl":"10.1177/19476035241308535","url":null,"abstract":"<p><strong>Introduction: </strong>Distal femoral cartilage lesions can be treated using osteochondral autograft transfer (OAT). When impacting plugs into a recipient site, the cartilage may fracture. This study aimed to analyze OAT donor regions and impaction energies to identify characteristics that lead to fracture.</p><p><strong>Methods: </strong>Fifteen cadaver femurs were used with OAT plugs harvested from the following regions: lateral and medial trochlea (LT and MT), lateral and medial intercondylar notch (LIN and MIN). Plugs were impacted into a bone surrogate block using a custom anvil-type system with pre-determined impact heights; 30, 50, 70, and 90 mm. Each plug's cartilage was examined and determined to be intact or fractured. Chi-square was used to compare the rate of chondral fracture for each region.</p><p><strong>Results: </strong>In all, 221 plugs were included. The overall rate of chondral fracture was 45.7%. There was a significant difference in the rate of fracture between regions, with LIN, MIN, LT, and MT, having a fracture rate of 46.6%, 62.7%, 25.0%, and 51.9%, respectively (<i>P</i> = 0.001). An impact height of 30 mm resulted in a fracture rate of 17.7%. Increasing the impact height from 30 to 50 mm resulted in significantly increased chondral fracture risk (<i>P</i> = 0.001).</p><p><strong>Conclusion: </strong>Different donor regions have varying rates of chondral fracture during OAT plug impaction, with the lateral and medial trochlea being the most resistant to chondral fracture at lower forces. Increased impact energy increases risk of chondral fracture. Surgeons should maintain caution and utilize lower impact energy when inserting OAT plugs.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241308535"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Associated Factors of Ankle Osteoarthritis in Achondroplasia: A Retrospective Radiographic Assessment of 134 Patients.","authors":"Won Seok Choi, Jung Woo Shin, Sei Wook Son, Hak Jun Kim, Sang Hyeon Hwang, Sang Geon Park, Young Hwan Park","doi":"10.1177/19476035241307862","DOIUrl":"10.1177/19476035241307862","url":null,"abstract":"<p><strong>Objective: </strong>The primary aim of this study is to examine the prevalence of ankle osteoarthritis in patients with achondroplasia and to assess the impact of surgical correction of lower limb alignment on this prevalence. The secondary aim is to identify radiographic parameters associated with ankle osteoarthritis.</p><p><strong>Design: </strong>This retrospective cohort study included 134 patients (268 ankles) who visited our institution between March 2014 and February 2023. Lower limb alignment was assessed using the mechanical axis deviation, lateral distal tibial angle, anterior distal tibial angle, and talar tilt angle. Ankle osteoarthritis was evaluated using the Van Dijk Osteoarthritis score and modified Kellgren-Lawrence (K-L) scale by 2 experienced orthopedic surgeons.</p><p><strong>Results: </strong>The prevalence of ankle osteoarthritis in our study cohort was 29%, and that of advanced ankle osteoarthritis defined by Van Dijk Osteoarthritis scale III or modified K-L grade 4 was 0.8% (aged <40 years, 22%, and 0%; aged ≥40 years, 42% and 2%, respectively). Talar tilt angle was the only radiographic parameter that showed a significant correlation with the degree of ankle osteoarthritis (Van Dijk Osteoarthritis score, r_s = 0.403, <i>P</i> < 0.001; modified K-L grade, r_s = 0.385, <i>P</i> < 0.001).</p><p><strong>Discussion: </strong>The prevalence of ankle osteoarthritis in achondroplasia is moderate, but the progression to advanced stages is uncommon. Ankle osteoarthritis in achondroplasia is strongly associated with increased talar tilt angle. This finding emphasizes caution regarding ankle osteoarthritis in achondroplasia with a nonparallel tibiotalar articular surface. Clinicians should, therefore, consider regularly assessing the talar tilt angle in patients with achondroplasia.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241307862"},"PeriodicalIF":2.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Return to Sport in Professional Athletes After Cartilage Restoration Surgery of the Knee: A Systematic Review and Meta-Analysis Demonstrates Gender Inequality and the Need for Improved Reporting.","authors":"Kyle N Kunze, Robert N Uzzo, Zach D Thomas, Justin Hicks, Scott A Rodeo, Riley J Williams","doi":"10.1177/19476035241292793","DOIUrl":"10.1177/19476035241292793","url":null,"abstract":"<p><strong>Objective: </strong>To synthesize the literature concerning return to sport (RTS) and related outcomes after cartilage restoration surgery of the knee in professional athletes.</p><p><strong>Design: </strong>Cochrane, PubMed, and OVID/Medline databases were queried for data pertaining to RTS after knee cartilage surgery in professional athletes. Demographic information, cartilage lesion characteristics, and RTS-specific information were extracted. Freeman-Tukey Double-Arscine Transformations with Dersimonian-Laerd random-effects estimators were constructed to quantitatively describe the cumulative incidence of RTS, while heterogeneous data described narratively.</p><p><strong>Results: </strong>Eleven studies (476 athletes; mean age 27.5 ± 2.1 years; 96.6% male) were included. Nine (81.8%) studies investigated a form of microfracture as treatment, with 6 (54.5%) performing isolated microfracture. The remaining studies investigated osteochondral allograft transplantation and mosaicplasty. More than half (<i>n</i> = 6; 54.5%) did not report cartilage lesion location or size. The pooled RTS rate was 84.3% (95% CI: 75.4%-91.8%) at a mean 39.9 (range, 12-104) weeks postoperatively. In 6 studies reporting competition level, a trend toward returning to a lower than pre-injury level was observed. The definition of RTS was only provided in 6 (54.5%) studies, while the criteria for RTS was only reported in 2 (18.2%) studies, suggesting limited transparency. One study reported an objective imaging assessment of reparative tissue, while none reported formal RTS testing protocols or minimum RTS timeline.</p><p><strong>Conclusion: </strong>Although the majority of professional athletes are reported to achieve a successful RTS after cartilage restoration surgery of the knee, the literature predominantly reflects microfracture treatment. Current limitations in this literature include a substantial lack of female representation and infrequent reporting of cartilage lesion characteristics, rehabilitation and RTS criteria, and objective imaging assessments of reparative tissue.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241292793"},"PeriodicalIF":2.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Second-Look Needle Arthroscopy After Prior Surgical Treatment for Cartilage Lesions of the Ankle: The Amsterdam and New York City Perspectives.","authors":"Alex B Walinga, James Butler, Jari Dahmen, Sjoerd A S Stufkens, Guillaume Robert, John G Kennedy, Gino M M J Kerkhoffs","doi":"10.1177/19476035241306550","DOIUrl":"10.1177/19476035241306550","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this prospective study was to evaluate the quality of the reparative cartilage during second-look needle arthroscopy following talar osteoperiostic grafting from the iliac crest (TOPIC) or autologous osteochondral transplantation (AOT) procedure for the management of large osteochondral lesions (OCLs) of the talus.</p><p><strong>Design: </strong>Prospective case series.</p><p><strong>Methods: </strong>Patients who underwent second-look needle arthroscopy following either TOPIC or AOT procedure were prospectively recruited when they needed a second look. The primary outcome was the assessment of the quality of the reparative cartilage via second-look needle arthroscopy scored by the International Cartilage Repair Society (ICRS) score. The secondary outcomes were the number and nature of needle arthroscopy interventions and complications associated with these interventions.</p><p><strong>Results: </strong>Five patients underwent second-look needle arthroscopy following TOPIC procedure and 11 patients underwent second-look in-office needle arthroscopy following AOT. The mean ICRS in the TOPIC cohort was 9.4 ± 1.0 at a mean time of 24.4 months following the index procedure. The mean ICRS in the AOT cohort was 10.6 ± 1.3 at a mean time of 58.8 months following the index procedure. No complications were observed in either cohort.</p><p><strong>Conclusion: </strong>This study demonstrated that TOPIC and AOT lead to adequate-looking quality reparative cartilage at short-term to mid-term follow-ups. However, further studies with larger patient cohorts and longer follow-ups are warranted. Furthermore, second-look needle arthroscopy is a safe and viable minimally invasive procedure that can effectively evaluate the quality of reparative cartilage following surgical intervention for OCLs of the talus.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241306550"},"PeriodicalIF":2.7,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Results after Autologous Matrix-Induced Chondrogenesis for Osteochondral Lesions of the Talus: A 10-Year Cohort Study.","authors":"Lukas Deiss, Markus Walther, Kathrin Pfahl, Hubert Hörterer, Alexander Mehlhorn, Anke Röser, Oliver Gottschalk","doi":"10.1177/19476035241301896","DOIUrl":"10.1177/19476035241301896","url":null,"abstract":"<p><strong>Objective: </strong>A gold standard surgical treatment for osteochondral lesions (OCLs) of the talus still needs to be established. Still, autologous matrix-induced chondrogenesis (AMIC) is a commonly applied 1-stage procedure that has achieved good short- and mid-term results. The present cohort study aimed to assess whether the long-term, 10-year results can confirm the previous findings.</p><p><strong>Design: </strong>All patients underwent an open AMIC procedure using a collagen type I/III bilayer matrix for a talar OCL. General demographic data, preoperative magnetic resonance imaging findings, intraoperative details, and German version of the Foot Function Index (FFI-D) scores preoperatively and at 1, 5, and 10 years as well as European Foot and Ankle Society (EFAS) and American Orthopedic Foot & Ankle Society (AOFAS) scores at 10 years after surgery were analyzed. The primary outcome variable was the procedure's longitudinal effect, and several variables' influence on the outcome was tested.</p><p><strong>Results: </strong>Of 47 consecutive patients, 18 (38%) were included. Of the 18 patients, 6 (33%) were female, and 12 (67%) were male, with a mean age of 39 ± 15 (range = 15-62) and an average body mass index (BMI) of 26 ± 5 (range = 20-38) kg/m². The mean defect size was 1.4 ± 0.9 (range = 0.2-4) cm². The FFI-D total score showed a significant decrease from preoperatively to 1 year postoperatively (56 ± 19 to 34 ± 27; <i>P</i> = 0.001) with a further nonsignificant decrease to the 5-year (34 ± 27 to 21 ± 20; <i>P</i> = 0.16) and 10-year follow-up (21 ± 20 to 15 ± 13; <i>P</i> = 1.00). All the single items decreased significantly from preoperatively to the 5- and 10-year mark. Although not significant, most items improved from 5 to 10 years postoperatively. Age positively correlated with the preoperative, 5-year, and 10-year follow-up FFI-D total score.</p><p><strong>Conclusions: </strong>AMIC, as a single-step surgical intervention, is a viable long-term treatment option. Patient selection regarding symptoms and findings is vital to achieve satisfying results.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241301896"},"PeriodicalIF":2.7,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11635787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Effects of Biomechanical Loading in Adolescent Human Growth Plate Cartilage: A Pilot Study.","authors":"Zhengpei Zhang, Nageswara Rao Boggavarapu, Laila Sara Arroyo Muhr, Ainhoa Garcia-Serrango, Tim Rj Aeppli, Tobia Sebastiano Nava, Yunhan Zhao, Elena M Gutierrez-Farewik, Artem Kulachenko, Lars Sävendahl, Farasat Zaman","doi":"10.1177/19476035241302954","DOIUrl":"10.1177/19476035241302954","url":null,"abstract":"<p><strong>Objective: </strong>The genomic effects of biomechanical loading on human growth plate cartilage are unknown so far. To address this, we used rare human growth plate biopsies obtained from children undergoing epiphysiodesis and exposed them to precisely controlled mechanical loading using a microloading device. The biopsies were cultured 24 hours after mechanical loading, followed by RNA-sequencing analyses to decipher the genomic regulation.</p><p><strong>Design: </strong>We conducted RNA-seq analysis of human growth plate cartilage obtained from three patients cultured <i>ex vivo</i> and subjected to cyclical mechanical loading with peak 0.4 N with frequency 0.77 Hz during a 30-second duration, using a specialized microloading device.</p><p><strong>Results: </strong>Gene ontology analysis revealed novel data showing three significantly upregulated signaling pathways, including notch, oxytocin, and tight junction, and three significantly downregulated signaling pathways, including lysosome, sphingolipid metabolism, and peroxisome proliferator-activated receptor (PPAR) in human growth plate cartilage. Moreover, we found 15 significantly regulated genes within these signaling pathways from all three patients. These genes included PSEN2, HEY1, and NCOR2 from the notch signaling; CACNB1 and PPP3R2 from the oxytocin signaling; ACTR3C, WHAMM, and ARHGEF18 from the tight junction signaling; ARSA, SMPD1, and CD68 from the lysosome signaling; ARSA and SMPD1 from the sphingolipid metabolism signaling; and SLC27A4 and AQP7 from the PPAR signaling pathway. In addition, 20 significantly upregulated genes and six significantly downregulated genes shared between two patient samples were identified.</p><p><strong>Conclusion: </strong>Our study provides the first-ever transcriptomic data of mechanical loading of human growth plate cartilage. These findings can potentially provide genetic targets for future investigations in physiological and pathological bone growth conditions.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035241302954"},"PeriodicalIF":2.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}