Cardiovascular Pathology最新文献

{"title":"Variable regional histomorphology of the ascending aorta: Implications for disease classification","authors":"Collin S. Pryma ,&nbsp;Md. Shahrier Amin ,&nbsp;Charles Leduc ,&nbsp;Cecilia Wu ,&nbsp;Sarah M. Jenkins ,&nbsp;Melanie C. Bois ,&nbsp;Joseph J. Maleszewski","doi":"10.1016/j.carpath.2025.107787","DOIUrl":"10.1016/j.carpath.2025.107787","url":null,"abstract":"<div><div>Regional differences in the normal histology of the ascending aorta have not been systematically studied, possibly resulting in diagnostic misinterpretation. This study aims to characterize the normal histologic spectrum of the aortic sinus (AS) and tubular aorta (TA) in a normal population. Ascending aortic specimens from 37 autopsy cases (mean age 58.7 years, range 12 to &gt;89 years) without known aortopathy were collected prospectively. Transverse and longitudinal sections from AS and TA were stained with H&amp;E and Verhoeff–Van Gieson. Assessed features included medial and lamellar thickness, lamellar architecture, elastic lamina number, elastic fiber waviness and organization, and features of medial degeneration. Interobserver agreement was assessed using Krippendorff’s alpha. The AS had significantly thinner media and lamellae, more woven lamellar architecture, and greater elastic fiber waviness and non-parallel organization compared to the TA. Elastic lamina number was greater in the posterior TA than AS (p &lt; 0.0001). Pathologist agreement was moderate to satisfactory except for lamellar architecture in the left and posterior AS. No significant differences were observed between the measures with age, sex, or body mass index up to 49 kg/m². These results provide the first systematic histologic profile of the AS and highlight key differences from the TA—several of which overlap with degenerative changes. Recognizing these normal regional variations is essential to avoid diagnostic overcalls and unnecessary intervention. Accurate specimen labeling, orientation, and sampling are critical in aortic pathology assessment.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107787"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVER 4: Table of Contents/Barcode PMS 200","authors":"","doi":"10.1016/S1054-8807(25)00080-8","DOIUrl":"10.1016/S1054-8807(25)00080-8","url":null,"abstract":"","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107795"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of C4d positivity within the first month after heart transplantation in detecting antibody-mediated rejection on endomyocardial biopsies","authors":"Mengxue Zhang ,&nbsp;Yan Zhou ,&nbsp;Chrystalle Katte Carreon ,&nbsp;Ann Nguyen ,&nbsp;Tiana Riley ,&nbsp;Aliya N. Husain ,&nbsp;Huihua Li","doi":"10.1016/j.carpath.2025.107783","DOIUrl":"10.1016/j.carpath.2025.107783","url":null,"abstract":"<div><h3>Background</h3><div>The pathologic definition for antibody-mediated rejection (AMR) includes both histopathological and immunopathological components. C4d is the most validated diagnostic marker for immunopathologic AMR; however, the clinical significance of early C4d positivity (≤1 month post-transplant) on endomyocardial biopsies (EMBs) is unknown.</div></div><div><h3>Methods</h3><div>Patients who had ≥1 episode of C4d-positive EMB within the first month after heart transplantation were selected, the coexistence with acute cellular rejection (ACR) and the correlations of C4d positivity with histopathologic features of AMR, clinical graft dysfunction, presence of donor specific antibodies (DSAs), and clinical outcomes were examined.</div></div><div><h3>Results</h3><div>112 EMBs from 46 patients were qualified and included in the study. 19 patients had single C4d-positive EMB whereas 27 patients developed multiple (2-4) episodes of C4d positivity within the first month. 40 % of C4d-positive EMBs showed concurrent ACR (26 with G1R, 6 with G2R). The C4d positivity correlated well with the histopathologic AMR, with 73 % of C4d-positive EMBs showing all or partial histologic features of AMR. Only 29 % of the C4d-positive EMBs were associated with clinical graft dysfunction, indicating that most early C4d-positive EMBs were clinically asymptomatic. DSAs were found positive in 28 patients (61 %), with preformed DSAs being more common than de novo DSAs. Although no cardiac allograft vasculopathy was observed in any patient, two pediatric patients died of AMR shortly after transplantation whereas three adult patients passed away mostly because of infection.</div></div><div><h3>Conclusion</h3><div>Heart transplant recipients with C4d-positive EMBs within the first month post-transplant were mainly asymptomatic; combined evaluation including clinical, pathological, and serological testing should be conducted for the best management of AMR.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107783"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dilemma of endomyocardial biopsy sampling for lymphocytic myocarditis diagnosis","authors":"Tatsuya Shiraki,&nbsp;Rika Kawakami,&nbsp;Renu Virmani","doi":"10.1016/j.carpath.2025.107778","DOIUrl":"10.1016/j.carpath.2025.107778","url":null,"abstract":"","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107778"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVER 3: Editorial Board","authors":"","doi":"10.1016/S1054-8807(25)00079-1","DOIUrl":"10.1016/S1054-8807(25)00079-1","url":null,"abstract":"","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107794"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological capillary analysis of ischemia with non-obstructive coronary arteries in a Fabry disease patient receiving enzyme replacement therapy","authors":"Hiromitsu Kanamori,&nbsp;Genki Naruse,&nbsp;Shingo Minatoguchi,&nbsp;Maya Ishiguro,&nbsp;Akihiro Yoshida,&nbsp;Hiroyuki Okura","doi":"10.1016/j.carpath.2025.107786","DOIUrl":"10.1016/j.carpath.2025.107786","url":null,"abstract":"<div><div>Fabry disease is a lysosomal disorder caused by an α-galactosidase A deficiency, which often causes angina in the absence of epicardial coronary artery stenosis. It is conceivable that epicardial and microvascular coronary dysfunction due to globotriaosylceramide and related glycoshingolipid accumulation within the vasculature causes ischemia with non-obstructive coronary arteries (INOCA). However, the precise histology remained unknown. We present the case of a 44-year-old male diagnosed with Fabry disease and treated with enzyme replacement therapy (ERT), who was referred to our cardiology department after complaining of exertional and rest precordial discomfort. Electrocardiography showed left ventricular hypertrophy (LVH) with strained ST segment depression. Echocardiography showed normal wall motion with diffuse LVH. Although coronary angiography showed no organic stenosis, spasms were provoked by intracoronary ergonovine injection. Invasive assessment of the microvasculature physiology showed the mean index of microcirculatory resistance to be 42 and the mean coronary flow reserve to be 1.6, which is indicative of coronary microvascular dysfunction. Endomyocardial biopsy revealed abundant accumulation of lamellar bodies within both cardiomyocytes and capillary pericytes, but not endothelial cells, suggesting microvascular flow disruption attributable to pericyte contraction and capillary constriction. This case highlights INOCA associated with a microvascular lesion related to Fabry disease in a patient receiving ERT as well as the need to develop therapies targeting the capillary circulation.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107786"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Giovanni Maria Lancisi (1654-1720) and the first historical investigation on pathology of sudden death","authors":"Daniela Marrone ,&nbsp;Cristina Basso ,&nbsp;Gaetano Thiene","doi":"10.1016/j.carpath.2025.107780","DOIUrl":"10.1016/j.carpath.2025.107780","url":null,"abstract":"<div><div>Between 1705 and 1706, Rome experienced a series of sudden deaths, prompting Pope Clement XI to set up a commission to investigate the causes. Giovanni Maria Lancisi, a prominent physician, oversaw forensic autopsies and wrote “De subitaneis mortibus” (“On Sudden Deaths”, 1708), a groundbreaking treatise that examined death through a mechanistic lens, offering both theoretical and practical insights. Lancisi’s book presented life as a dynamic interaction of bodily fluids and systems, with death defined as the cessation of these movements. He proposed that sudden death is not rare but a natural endpoint when life-sustaining processes abruptly cease. The treatise identified various causes of sudden death, involving heart and vessels, and debunked fears of an ongoing epidemic in Rome. By analyzing cadaveric lesions, Lancisi demonstrated that these deaths were primarily due to pre-existing morbid conditions. His observations advanced the emerging field of pathological anatomy, applying scientific method on the study of sudden death.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107780"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circ_0005372 targets the miR-153-3p/ITGB3 axis to stimulate the PI3K/AKT signaling pathway to facilitate the occurrence and development of congenital heart disease and pulmonary arterial hypertension in children","authors":"Mingyan Zhu ,&nbsp;Guixiang Li ,&nbsp;Yungui Li ,&nbsp;Jinsong Chen","doi":"10.1016/j.carpath.2025.107768","DOIUrl":"10.1016/j.carpath.2025.107768","url":null,"abstract":"<div><h3>Background</h3><div>Congenital heart disease (CHD) is the most common and highest incidence of congenital malformations in newborn infants. Pulmonary arterial hypertension (PAH) is the most serious complication associated with CHD. It is a great threat to the safety and health of newborns.</div></div><div><h3>Methods</h3><div>The expression of circ_0005372, miR-153-3p, and ITGB3 were detected by Real-time quantitative PCR (RT-qPCR) and western blot (WB). MTT and EdU assays were used to evaluate the viability and proliferation of human pulmonary artery smooth muscle cells (HPASMC). Migration and invasion abilities were determined by wound healing and Transwell assays. Pull down and RNA Immunoprecipitation (RIP), and dual luciferase reporter gene assays were used to detect targeting relationships of circ_0005372, miR-153-3p, and ITGB3.</div></div><div><h3>Results</h3><div>Plasma samples from 27 healthy, CHD, and PAH<img>CHD pairs of newborns were collected. Circ_0005372 was highly expressed in PAH<img>CHD patient samples and HPASMCs under hypoxic conditions. Knockdown of circ_0005372 inhibited HPASMCs viability, proliferation, migration, and invasion. Circ_0005372 targeted miR-153-3p and showed a negative correlation. The inhibitory effect of silencing circ_0005372 on the HPASMCs was reversed by miR-153-3p inhibitor. MiR-153-3p negatively regulated ITGB3, and overexpression of ITGB3 abolished the effect of miR-153-3p in hypoxia-treated HPASMCs. Circ_0005372 could regulate the PI3K/AKT signaling pathway through miR-153-3p/ ITGB3.</div></div><div><h3>Conclusion</h3><div>In summary, circ_0005372 mediates the expression of ITG3B via targeting miR-153-3p, thereby stimulating the PI3K/AKT signaling pathway, helping the proliferation, migration, and invasion of HPASMCs and accelerating the deterioration of PAH<img>CHD.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107768"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aortic valve morphology rather than aortic valve function, aortic dilatation, and age interferes with ascending aortic structural and biomechanical properties","authors":"Jan M. Federspiel ,&nbsp;Jan-Christian Reil ,&nbsp;Peter H. Schmidt ,&nbsp;Paul Teping ,&nbsp;Frank Ramsthaler ,&nbsp;Tanja Schwab ,&nbsp;Hans-Joachim Schäfers","doi":"10.1016/j.carpath.2025.107782","DOIUrl":"10.1016/j.carpath.2025.107782","url":null,"abstract":"<div><div>Aortic valve (AV) malformation and AV malfunction have been linked to aortic wall degeneration. Studies concomitantly assessing AV morphology, AV function, age, ascending aortic dilatation, and aortic biomechanical properties are lacking. This exploratory study aims to close this gap. Surgical samples of the ascending aorta (n=102) were histologically assessed. Based on echocardiographic studies, the elastic modulus (slope stress-strain curve) was calculated. Patient characteristics were collected from the patient charts. Samples obtained during autopsy (n=10) served as reference for the microscopic analysis. The patient characteristics, structural aortic wall changes, and biomechanical wall properties were statistically explored using comparative analyses and a Spearman correlation matrix. Marked medial degeneration was found significantly earlier in life for unicuspid AV morphology compared to bicuspid and tricuspid AV. Significantly fewer lamellar units and thinner aortic walls were found in surgical samples compared to the reference group regardless of AV morphology, AV function, age, and aortic dilatation. Adventitial structural impairment was associated with stiffer aortic walls. Hints were found that AV morphology (rather than AV function, age, and presence/absence of aortic dilatation) affects structural and functional ascending aortic wall properties. Additionally, the observations suggest more advanced aortic degeneration in association with unicuspid AV, underpin the need for non-surgical control samples in surgical pathological studies, and highlight the importance of the adventitial layer for aortic biomechanics.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107782"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aortic wall lamellar structure in phylogeny and in humans: insights from bicuspid and tricuspid aortic valve morphology","authors":"Nora Bacour ,&nbsp;Mohammad Zafar ,&nbsp;Nicole Kargin ,&nbsp;Bulat Zinganshin ,&nbsp;Robert Poelmann ,&nbsp;Robert J.M. Klautz ,&nbsp;John Elefteriades ,&nbsp;Nimrat Grewal","doi":"10.1016/j.carpath.2025.107789","DOIUrl":"10.1016/j.carpath.2025.107789","url":null,"abstract":"<div><h3>Objective</h3><div>Despite a voluminous literature on aortopathy, comparatively little attention has been paid to the lamellar architecture of the aortic wall, including the number and distribution of these layers. This study compares the lamellar organization of the developing aorta in individuals with a bicuspid (BAV) versus tricuspid aortic valve (TAV) and includes a literature review on aortic lamellae in mammals and adult humans.</div></div><div><h3>Methods</h3><div>Non-dilated ascending aortic wall samples (<em>n</em> = 83) were analyzed from individuals aged embryonic to 72 years, categorized into six age groups. Additionally, a literature review was carried out on lamellar counts across species.</div></div><div><h3>Results</h3><div>The elastic lamellae in preterm aortas were well-structured with no pathological features. Conversely, adult aortas showed progressive elastic fiber pathology. The number of lamellae increased with age; neonates had the lowest count, peaking in young children, and gradually decreasing in adolescents. Furthermore, compared to TAV patients, BAV patients showed patterns of thinner intimal layers and fewer elastic lamellae. Our literature review showed that the quantity of lamellae in mammals is correlated with both body size and aortic radius, with smaller animals having fewer lamellae. The ascending thoracic aorta in humans had between 45 and 78 elastic lamellae, while the descending and abdominal aortas had fewer layers.</div></div><div><h3>Conclusion</h3><div>The number of elastic lamellae in the aortic wall is influenced by both age and valve morphology. BAV patients had less lamellae than TAV patients, which could contribute to the disparity in clinical outcomes. In older adults, age-related lamellar degeneration likely increases the risk of aortopathy.</div></div>","PeriodicalId":9451,"journal":{"name":"Cardiovascular Pathology","volume":"80 ","pages":"Article 107789"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145430418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}