Biotechnologia最新文献

{"title":"Microbial canthaxanthin: an orange-red keto carotenoid with potential pharmaceutical applications.","authors":"Vinita Gaur,&nbsp;Surojit Bera","doi":"10.5114/bta.2023.130733","DOIUrl":"10.5114/bta.2023.130733","url":null,"abstract":"<p><p>Canthaxanthin is an orange-red keto-carotenoid that occurs naturally and is also manufactured by synthetic methods for regular applications. In nature, canthaxanthin mainly exists in microbes such as different bacterial species, fungi, and algae, as well as in animals such as crustaceans, certain fishes, and birds. However, the amount of canthaxanthin produced in these organisms varies significantly. Additionally, the compound can be generated from genetically modified organisms using genetic engineering techniques Canthaxanthin finds extensive application as an additive in animal feed, in the pharmaceutical industry, as a coloring agent for various food products, and in cosmetics. It has powerful antioxidant properties and plays a role in lipid metabolism, neuroprotection, and immunomodulation. This article gives an extensive insight into the structure and methods of synthesis of canthaxanthin along with its various newly discovered sources identified so far. The significant applications of canthaxanthin, particularly its role in pharmaceuticals, are critically evaluated. Furthermore, the article discusses future aspects and challenges associated with canthaxanthin production and regulation.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 3","pages":"315-328"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ba/01/BTA-104-3-51306.PMC10578118.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of <i>Enterococcus italicus</i> ONU547 on the growth and acclimatization of micropropagated <i>Rubus fruticosus</i> L. and <i>Paulownia tomentosa</i> Steud. plants to <i>ex vitro</i> conditions.","authors":"Nadiia Tytarenko,&nbsp;Nataliia Tesliuk,&nbsp;Andrii Merlich,&nbsp;Thomas Haertlé,&nbsp;Volodymyr Ivanytsia","doi":"10.5114/bta.2023.130732","DOIUrl":"10.5114/bta.2023.130732","url":null,"abstract":"<p><p>Clonal micropropagation is an effective method for plant reproduction, applicable in both scientific and industrial domains. However, a significant number of microclones are lost during the <i>ex vitro</i> acclimatization process. To address this, the introduction of beneficial microorganisms into the rhizosphere of micropropagated plants could have a positive effect on the survival rates and external characteristics of acclimatized plantlets. The aim of this study was to determine the protective and growth-promoting potential of <i>Enterococcus italicus</i> ONU547 and its effect on micropropagated plants during acclimatization. The antagonistic activity of the bacteria was determined using the agar block method. <i>Lepidium sativum</i> L. seeds were inoculated with bacterial suspensions at concentrations of 10⁶, 10⁷, and 10⁸ CFU/ml. Subsequently, the roots of the microclones were treated with suspensions of 10⁶ and 10⁷ CFU/ml, and biometric characteristics were measured. The results demonstrated antagonistic properties against various phytopathogenic fungi, including <i>Aspergillus niger</i>, <i>Cladosporium cladosporioides</i>, <i>Alternaria alternata</i>, <i>Alternaria tenuissima</i>, <i>Rhizoctonia cerealis</i>, <i>Penicillium expansum</i>, and <i>Paecilomyces variotii</i>. Inoculation of <i>L. sativum</i> L. seeds resulted in improved germination rates, increased root numbers, and enhanced root and shoot lengths. Similarly, the effects of the studied bacteria on <i>Rubus fruticosus</i> L. and <i>Paulownia tomentosa</i> Steud. during the acclimatization stage led to higher survival rates, increased shoot lengths, greater node numbers, and larger leaf areas. A concentration of 10⁷ CFU/ml was identified as optimal for inoculating the microclones. The findings indicate that <i>E. italicus</i> ONU547 holds promise for the inoculation of micropropagated plants during the acclimatization process. Further research is recommended to establish the specific interaction mechanisms between these bacteria and plants.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 3","pages":"301-313"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/2d/BTA-104-3-51305.PMC10578123.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiochemical response of <i>Cicer arietinum</i> to zinc-containing mesoporous silica nanoparticles under water stress.","authors":"Maryam Mohamadzadeh,&nbsp;Mohsen Janmohammadi,&nbsp;Amin Abbasi,&nbsp;Naser Sabaghnia,&nbsp;Viorel Ion","doi":"10.5114/bta.2023.130729","DOIUrl":"10.5114/bta.2023.130729","url":null,"abstract":"<p><p>Chickpea is an important food legume cultivated in semiarid regions, where water scarcity and nutrient deficiencies negatively affect crop production. This study aimed to investigate the effect of zinc and silicon from different sources, including bulk and nanostructures, on various biochemical traits of chickpea plants grown under field conditions in Maragheh, Northwest Iran. The main experimental factor consisted of three soil moisture levels: irrigation to 90% of field capacity (FC), 60% FC, and 30% FC. The subplots were assigned for foliar application of different fertilizers: control (distilled water), zinc sulfate (ZnSO), silicon dioxide nanoparticles (SiO₂ NPs), ZnSO + SiO₂ NPs, and zinc-containing mesoporous silica nanoparticles (MSNPs -Zn). The results showed that although decreased soil moisture had a negative impact on several biochemical processes, foliar application of Zn and Si in both conventional bulk and nanostructure significantly affected plant antioxidant system, plasma membrane integrity, and the concentrations of photosynthetic pigments and compatible solutes. However, the most inducing effects on catalase, ascorbate peroxidase, guaiacol peroxidase, superoxide dismutase, and anthocyanin were observed with the foliar spray of MSNPs-Zn and ZnSO + SiO₂ under 60% FC. Moreover, foliar spray of MSNPs-Zn alleviated the negative effects of water deficit stress on photosynthetic pigments (chlorophyll <i>a</i> /<i>b</i> and carotenoid content). Water stress significantly induced the accumulation of free proline in the leaves. Overall, the results indicated that foliar spray of MSNPs -Zn, especially under 60% FC, improved the plant's defense system, scavenged reactive oxygen species, and enhanced the accumulation and stability of pigments, thereby mitigating the effects of drought stress.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 3","pages":"263-273"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/e9/BTA-104-3-51302.PMC10578114.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of the gene encoding blood coagulation factor VIII without domain B in <i>E. coli</i> bacterial expression system.","authors":"Anna Mazurkiewicz-Pisarek,&nbsp;Alina Mazurkiewicz,&nbsp;Diana Mikiewicz,&nbsp;Piotr Baran,&nbsp;Tomasz Ciach","doi":"10.5114/bta.2023.130728","DOIUrl":"10.5114/bta.2023.130728","url":null,"abstract":"In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 3","pages":"247-262"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c5/b5/BTA-104-3-51301.PMC10578111.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (<i>Chrysanthemum morifolium</i> Ramat) callus culture based on harvest time differences.","authors":"Tia Setiawati,&nbsp;Annisa N Arofah,&nbsp;Mohamad Nurzaman,&nbsp;Annisa Annisa,&nbsp;Asep Z Mutaqin,&nbsp;Rusdi Hasan","doi":"10.5114/bta.2023.130731","DOIUrl":"10.5114/bta.2023.130731","url":null,"abstract":"<p><p>Chrysanthemum (<i>Chrysanthemum morifolium</i>) contains secondary metabolites, such as flavonoid compounds, especially luteolin-7-glucoside and quercetin-3-O-rhamnoside (quercitrin), in its tissues. Utilizing sucrose as an elicitor through callus culture presents an alternative method to enhance the production of secondary metabolites. This research aimed to determine the best sucrose concentration and harvest time for maximizing quercitrin content in chrysanthemum callus culture. The research employed a completely randomized design with four treatment groups: 0, 30, 45, and 60 g/l of sucrose added to MS medium containing 4 ppm 2,4-dichlorophenoxyacetic acid (2,4-D). Callus samples were harvested on the 15th and 30th days of culture. The observed parameters included callus morphology (color and texture), fresh weight, dry weight, the diameter of the callus, and quercitrin content analyzed using high-performance liquid chromatography. The results showed that all callus cultures exhibited intermediate textures and varied colors, predominantly shades of brown. The treatment involving 45 g/l of sucrose with a 30th-day harvest yielded the highest fresh weight, dry weight, and quercitrin content, namely 2.108 g, 0.051 g, and 0.437 mg/g DW, respectively. Notably, the quercitrin content exhibited a 63.67% increase compared to the control.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 3","pages":"289-300"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d3/53/BTA-104-3-51304.PMC10578125.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of potential CD8+ epitopes in pp62 polyprotein of African swine fever virus using computational immunology.","authors":"Mark Lester C Galicia,&nbsp;Dale Jonathan M Morales,&nbsp;Precious Grace B Pogado,&nbsp;Ashley L Quebrado,&nbsp;Leana Rich Herrera-Ong","doi":"10.5114/bta.2023.130726","DOIUrl":"10.5114/bta.2023.130726","url":null,"abstract":"<p><p>The highly infectious African swine fever virus (ASFV) is currently the only known DNA arbovirus within the <i>Asfarviridae</i> family which primarily infects domestic pigs and wild boars. African swine fever (ASF) leads to a mortality rate of up to 100% which has caused massive socio-economic losses worldwide. Previous research indicates that ASFV's virulence can be attributed to polyprotein pp62, which plays a crucial role in viral assembly and core maturation. This particular study utilized <i>in silico</i> analysis to identify highly conserved cytotoxic T-cell epitopes in pp62 that can potentially serve as key components for future ASFV vaccines. To achieve this, the researchers retrieved, clustered, and aligned the peptide sequences of pp62. Subsequently, the aligned sequences were analyzed to identify epitopes that bind promiscuously to the swine major histocompatibility complex I (MHC I) alleles and exhibiting MHC IC₅₀ values < 500 nM. Additionally, peptide sequences with positive proteasome and TAP scores were considered. Potential cross-reactivity was assessed by comparing the peptide sequences against available proteome sequences of <i>Sus scrofa domesticus</i> in various databases. Furthermore, molecular docking was conducted to evaluate the binding of candidate epitopes with swine leukocyte antigen-1*0401 (SLA-1*0401). The dissociation constants, binding energies, root mean square deviation, and root mean square fluctuation values for the SLA-epitope complexes were compared with a positive reference. In the course of the study, 21 highly conserved CD8+ epitopes were identified, out of which four were further assessed for their potential immunogenicity. The results demonstrated that the highly conserved CD8+ epitopes discovered in this study are promising for integration into future ASFV vaccine formulations. As preliminary data, it is anticipated that these findings will subsequently undergo <i>in vitro</i> and <i>in vivo</i> studies in the future.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 3","pages":"221-231"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/92/c8/BTA-104-3-51299.PMC10578124.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41242921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}