Biotechnologia最新文献

{"title":"Impact of <i>Spirulina platensis</i> biomass on the viability of <i>Lactobacillus delbrueckii</i> subsp. <i>bulgaricus</i> strain during the freeze-drying process.","authors":"Ivo Ganchev","doi":"10.5114/bta.2024.139751","DOIUrl":"10.5114/bta.2024.139751","url":null,"abstract":"<p><p>In this work, we evaluated the protective capacity of <i>Spirulina platensis</i> biomass in preserving <i>Lactobacillus delbrueckii</i> subsp. <i>bulgaricus</i> WDCM 00102. The <i>L. bulgaricus</i> strain was freeze-dried in the presence of <i>S. platensis</i> biomass and the freeze-dried samples were then stored at 5 and 25°C for varying periods. Subsequently, the samples were rehydrated and bacterial plate counts were determined. The results indicate that a concentration of 12% <i>S. platensis</i> biomass was highly effective in preserving <i>L. bulgaricus</i>. Commercial products with higher <i>S. platensis</i> biomass content exhibited greater protective capacity. While <i>S. platensis</i> biomass is well-known for its prebiotic properties, its protective role has not been previously reported or thoroughly explored. This study demonstrates the protective capacity of <i>S. platensis</i> biomass in preserving <i>L. bulgaricus</i>, a strain particularly sensitive to preservation processes.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"109-119"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary metabolites, spectra characterization, and antioxidant correlation analysis of the polar and nonpolar extracts of <i>Bryophyllum pinnatum</i> (Lam) Oken.","authors":"Chinyere E Okafor, Ikechukwu K Ijoma, Chiamaka A Igboamalu, Chinaza E Ezebalu, Chukwuemeka F Eze, Jessica C Osita-Chikeze, Chisom E Uzor, Adaugo L Ekwuekwe","doi":"10.5114/bta.2024.139752","DOIUrl":"10.5114/bta.2024.139752","url":null,"abstract":"<p><p>Oxidative stress-related pathologies have guided the scientific community into delving into natural product-based research on plant-based metabolites. Plant secondary metabolites serve as a valid alternative in managing oxidative stress-related pathologies. In this study, we present the secondary metabolite constituents of the polar extract (PE) and nonpolar extract (NPE) from the leaves of <i>Bryophyllum pinnatum</i>. These constituents were determined through qualitative and quantitative phytochemical screening. The functional groups and structures of these metabolites were determined based on FTIR and GC-MS experiments, respectively. Antioxidant and free radical scavenging (FRS) activities were determined using standard methods, including phosphomolybdenum, FRAP, DPPH, HRSA, and reducing power assays, with comparisons made to the ascorbic acid (AA) standard. Through Pearson correlation analysis, we estimated the relationship between antioxidant and FRS activities. The DPPH results revealed IC50s of 380.104 ± 0.001, 16.763 ± 0.001, and 7.684 ± 0.003 μg/ml for NPE, AA, and PE, respectively, indicating a trend of PE > AA > NPE. However, all other experiments showed a trend of AA > PE > NPE in antioxidant and FRS activities. These results showed the potential antioxidant and FRS properties of both PE and NPE. Additionally, the correlation analysis indicated a strong positive correlation between the antioxidant and FRS activities of PE and NPE. The research results suggest high antioxidant and FRS activities of PE and validate the use of <i>B. pinnatum</i> in managing free radical-related pathologies.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"121-136"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological lactic acid production from low-cost renewable sources <i>via</i> anaerobic microbial processes.","authors":"Andriy Anta Kacaribu, Darwin","doi":"10.5114/bta.2024.139757","DOIUrl":"10.5114/bta.2024.139757","url":null,"abstract":"<p><p>Lactic acid (LA) production from microbial fermentation using low-cost renewable sources has emerged as an attractive alternative to the use of petroleum-based products. This approach not only offers sustainable solutions for waste management but also enables the production of value-added products in an eco-friendly manner. However, to make this approach economically viable, optimizing the production process for high yield, productivity, and purity while minimizing costs is crucial. To address these challenges, various approaches have been proposed, including the use of neutralizing agents, high cell density cultures, co-cultures, fed-batch fermentation, and product removal strategies. Overall, this review underscores the potential of microbial fermentation for LA production as a sustainable and cost-effective solution to meet the growing demand for eco-friendly products. Further optimization of fermentation processes and the development of new microbial strains and fermentation techniques are key to advancing this approach. The production of LA through microbial fermentation presents a sustainable and eco-friendly solution to the increasing demand for eco-friendly products. With continued innovation, we can expect to see a significant reduction in the environmental impact of industrial processes, coupled with a more cost-effective and high-purity source of lactic acid for various industries.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"179-194"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micropropagation of <i>Dipcadi montanum</i> (Dalz.) Baker (<i>Asparagaceae</i>): a rare scapigerous herb.","authors":"Sautrik Basu, Emadul Islam, Debraj Chakraborty","doi":"10.5114/bta.2024.139755","DOIUrl":"10.5114/bta.2024.139755","url":null,"abstract":"<p><p><i>Dipcadi montanum</i> (Dalz.) Baker (<i>Asparagaceae</i>) is a rare scapigerous herb endemic to the Western Ghats, a global biodiversity hotspot running parallel to the western coast of India. This study reports the development of a reproducible protocol for mass propagation of this underutilized geophyte using bulb scale and immature leaf base explants. Miniature bulblets were successfully induced from both types of explants after 4 and 8 weeks of culture on full-strength semisolid MS basal medium fortified with 3% sucrose and varying levels of BAP (4.4-17.7 μM) and TDZ (4.5-18.1 μM). The addition of 2.7 μM NAA further enhanced the rate of microbulb induction. Rooting of the 8-week-old bulblets, obtained from both explants, was achieved with more than 90% efficiency on liquid as well as agar-gelled half-strength MS basal medium fortified with varying levels of IBA (2.46-9.84 μM) and NAA (2.68-10.74 μM), with or without 2.32 μM Kinetin. More than 95% of the rooted plants survived the initial acclimatization process under controlled ex-vitro conditions, and a survival rate of over 80% was recorded after 4 weeks of transfer to greenhouse conditions. After a brief dormancy, the regenerants resumed growth in the postmonsoon season and exhibited morphological resemblance to the donor plant. Comparative cytological analysis between the donor and 15 randomly selected regenerants revealed a stable somatic count of 2<i>n</i> = 20.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"159-167"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetics of antibiotic resistance in methicillin-resistant <i>Staphylococcus aureus</i> (MRSA).","authors":"Klementyna Marciniak, Agata Tyczewska, Kamilla Grzywacz","doi":"10.5114/bta.2024.139756","DOIUrl":"10.5114/bta.2024.139756","url":null,"abstract":"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains pose a significant threat as common causes of bacterial infections in hospitals, often resistant to available antibiotics such as daptomycin, vancomycin, and linezolid. The continuous emergence of new MRSA isolates with no effective treatment options underscores a real threat to health among humans and animals, and the number of effective antibiotic therapies decreases with each passing year. In this review, we provide an overview of the most common genetic mechanisms of resistance to a broad spectrum of antibiotics in methicillin-resistant <i>S. aureus</i>.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"169-177"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effect of magnetic field on rapeseed growth and the causal agent of blackleg disease, <i>Phoma lingam</i>.","authors":"Samira Peighami Ashnaei, Reyhane Sadeghi, Laleh Hosseinian, Ahmad Shafaeizadeh, Mehran Zeinalipour, Hamid Keshvari, Mehrdad Imanzadeh, Mostafa Bahmanabadi","doi":"10.5114/bta.2024.139754","DOIUrl":"10.5114/bta.2024.139754","url":null,"abstract":"<p><p>In recent years, with the increased production of oilseed rape, there has been a simultaneous enhancement in reports on pathogens causing diseases. Magnetic technology has been recognized as a new agricultural method aimed at improving health and crop production. In this work, the effect of magnetic fields was studied on the mycelial growth and conidia formation of <i>Leptosphaeria maculans</i> Gol125 and <i>Leptosphaeria biglobosa</i> KH36, the causal agents of Phoma stem cancer (blackleg) disease in rapeseed. In addition, seeds exposed to eight direct frequencies of magnetic fields were impregnated with pathogen suspension and grown under greenhouse conditions. The growth speed of both pathogen isolates decreased by 1-28% in GOL125 and 6-46% in KH36 over time in cultures exposed to magnetic fields. However, the number of conidia increased significantly under magnetic field exposure, reaching 5.4 × 10⁷ and 7.7 × 10⁷ SFU/ml in KH36 and GOL125 isolates, respectively. Furthermore, in greenhouse conditions, an increase in photosynthetic pigment levels was observed in almost all of the magnetic field-treated plants. In addition, disease incidence decreased by around 6% in the magnetic field-treated plants. This study represents the first evaluation of magnetic technology in controlling plant diseases. The use of magnetic fields may present a viable strategy for a sustainable production system; however, it requires further advanced studies to improve plant health and productivity.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 2","pages":"149-158"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11231998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141582009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic <i>Aspergillii</i> and <i>Penicillii</i> from medicinal plants: a focus on antimicrobial and multidrug resistant pathogens inhibitory activity.","authors":"Jendri Mamangkey, Lucas William Mendes, Apon Zaenal Mustopa, Adrian Hartanto","doi":"10.5114/bta.2024.135644","DOIUrl":"https://doi.org/10.5114/bta.2024.135644","url":null,"abstract":"<p><p>The rise of multidrug resistance among microorganisms, where they develop resistance against formerly efficacious drugs, has led to increased disease prevalence and mortality rates, posing a growing challenge. Globally, antibiotic resistance has made a significant impact, causing millions of fatalities each year. Endophytic fungi have gained considerable attention in research due to their potential to produce a wide variety of secondary metabolites, including natural substances with antimicrobial capabilities. The genera <i>Aspergillus</i> and <i>Penicillium</i> stand out as the most prevalent species of endophytic fungi. Filamentous fungi, such as these are responsible for the production of 45% of known microbial metabolites. This review focuses on exploring the bioactive substances produced by endophytic fungi from these two genera, particularly in conjunction with medicinal plants. Emphasis is placed on their antimicrobial activity and their ability to inhibit multidrug-resistant pathogens. As the need for alternative treatments to combat drug-resistant infections continues to grow, endophytic fungi have the potential to provide a valuable source of bioactive molecules for medical applications.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"83-95"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage and time course effects on the quality of oil extracted from <i>Phyllanthus amarus</i> Schumach and <i>Annona muricata</i> Linn and their antidiabetic potentials.","authors":"Oluwasayo E Ogunjinmi, Vincent O Oriyomi, Richard A Olaogun, Amidat T Gbadegesin","doi":"10.5114/bta.2024.135641","DOIUrl":"https://doi.org/10.5114/bta.2024.135641","url":null,"abstract":"<p><p>With the advent of modern technology, advancements in processing and storage techniques, and increasing medical knowledge, people are becoming aware of deterioration in the quality of medicinal products due to storage methods and time. In most cases, herbal products are not consumed immediately after production; as such, improper storage can result in physical, chemical, and microbiological changes. The study evaluated the effect of storage methods and time on the quality of oil extracted from <i>Phyllanthus amarus</i> Schumach and <i>Annona muricata</i> Linn and assessed their antidiabetic and antioxidative effects. Plants were air-dried, pulverized, and then subjected to Soxhlet extraction in petroleum ether. The oil was evaluated for phytochemical constituents and the effects of time and storage methods on its physicochemical properties. Characterization of the oil was done by spectroscopic techniques. Oils from both plants contained tannins, flavonoids, alkaloids, steroids, glycosides, terpenoids, phlobotannins, resins, reducing sugar, phenols, and saponins in different proportions. The oil from <i>A. muricata</i> had higher phenolic (3.11 ± 0.31 mg GAE/g), flavonoid (11.82 ± 0.08 mg QUE/g), alkaloid (16.37 ± 0.56 mg APE/g), and tannin (7.13 ± 0.47 mg CE/g) contents than the oil from <i>P. amarus</i>, which had 0.54 ± 0.08 mg GAE/g, 7.83 ± 0.13 mg QUE/g, 9.87 ± 0.15 mg APE, and 3.16 ± 0.12 mg CE/g for total phenolic, flavonoids, alkaloids, and tannins, respectively. Initial acid, iodine, peroxide, and saponification values recorded for <i>P. amarus</i> were 5.63 ± 0.82 mg KOH/g, 97.17 ±0.53 Wijis, 9.31 ± 0.15 mEq/kg, and 116.11 ± 0.74 mg KOH/g, respectively, significantly different from those of <i>A. muricata</i> , which had values of 1.17 ± 0.08 mg KOH, 76.23 ± 0.03 Wijis, 6.75 ± 0.47 mEq/kg, and 193.31 ± 0.52 mg KOH/g, respectively. FT-IR characterization of the oils revealed the presence of carboxylic acid, alkyl, alkene, alkane, haloalkane, aldehyde, aromatic amine, α-unsaturated and β-unsaturated esters, and phenol functional groups. <i>P. amarus</i> oil inhibited α-amylase (IC₅₀ 0.17 ± 0.03 mg/ml), α-glucosidase (IC₅₀ 0.64 ± 0.03 mg/ml), and xanthine oxidase (0.70 ± 0.01 mg/ml) to a greater extent than <i>A. muricata</i> oil, with IC₅₀ values of 0.43 ± 0.05 mg/ml (α-amylase), 2.25 ± 0.31 mg/ml (α-glucosidase), and 0.78 ± 0.07 mg/ml (xanthine oxidase). This study showed that oils from the tested plants have low rancidity with a moderate shelf life. The extracts contained essential phytoconstituents that significantly inhibited α-glucosidase and xanthine oxidase. These effects of the oil indicate their potential to prevent diabetes, gout, and oxidative stress. Consequently, the supply of <i>P. amarus</i> and <i>A. muricata</i> in homemade diets is strongly encouraged for healthy living.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"41-53"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical characterization by GC-MS and <i>in vitro</i> evaluation of antiproliferative and antimigratory studies of <i>Leucas aspera</i> leaf extracts on MDA-MB-231 cell line.","authors":"Mahaboob Begum S M Fazeela, Megasri Sankarram","doi":"10.5114/bta.2024.135642","DOIUrl":"https://doi.org/10.5114/bta.2024.135642","url":null,"abstract":"<p><p>Breast cancer is the most recurrently identified and one of women's prominent causes of death. Currently, researchers have turned their focus on natural chemicals from synthetic chemicals due to their environmental, economic, and health benefits. Considering this, the medicinal plant <i>Leucas aspera</i> was chosen for the current study. The aim of this study was to isolate and characterize secondary metabolites from <i>L. aspera</i> and determine the antiproliferative and antimigratory activities in the MDA-MB-231 cell line under <i>in vitro</i> conditions. Phytochemicals from <i>L. aspera</i> were isolated through sequential extraction using hexane, dichloromethane, and ethyl acetate. These extracts were qualitatively screened, subjected to FT-IR, and analyzed using GC-MS. The antiproliferative activity was determined through the MTT assay. Scratch assay was utilized to determine the antimigratory activity of the plant extracts. The phytochemical analysis revealed the presence of steroids, alkaloids, phenols, flavonoids, galactose, tannins, saponins, and amino acids in the extracts. The results of the cell viability assay indicated that the crude dichloromethane and ethyl acetate extracts inhibited cell proliferation, with inhibitory concentrations of 5 and 3 μg/ml, respectively. In contrast, the crude hexane extract did not exhibit any cytotoxicity. Furthermore, the scratch assay results showed that the plant extracts had cell migration inhibitory properties. The outcomes of the current study conclude that <i>L. aspera</i> possesses active therapeutic agents with strong anticancer potential, effectively impeding the proliferation and invasion of MDA-MB-231. Further studies are needed to identify the potential active agents that contribute to these activities.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"55-68"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of thiacalix[4]arene C-1193 for a directed influence on the functional activity of mitochondria and simulation of this process using a Petri nets.","authors":"Hanna Danylovych, Yurii Danylovych, Alexander Chunikhin, Sergiy Cherenok, Vitaly Kalchenko, Sergiy Kosterin","doi":"10.5114/bta.2024.135643","DOIUrl":"https://doi.org/10.5114/bta.2024.135643","url":null,"abstract":"<p><p>In molecular biological studies, considerable attention is paid to macrocyclic nanoscale compounds known as calix[4]arenes. An imperative concern in biochemical membranology and molecular biotechnology is the exploration of effectors capable of modifying the intensity of redox reactions within the inner mitochondrial membrane and influencing the activity of its Ca²⁺ transport systems. The simulation model development is relevant to formalize and generalize the experimental data and assess the conformity of experimental results with theoretical predictions. Experiments were carried out on a suspension of isolated rat myometrial mitochondria. The synthesized thiacalix[4]arene C-1193, containing four sulfur atoms, was employed. Demonstrations of time-dependent and concentration-dependent (0.01-10 μM) inhibition of Ca²⁺ accumulation and reactive oxygen species (ROS) formation by mitochondria in the presence of C-1193 were observed. While C-1193 inhibited the oxidation of NADH and FADH₂, it did not induce mitochondrial swelling. The thiacalix[4]arene also inhibited the synthesis of nitric oxide, with a Ki of 5.5 ± 1.7 nM, positioning it as a high-affinity blocker of endogenous NO generation in mitochondria. These results are the basis for the possible application of the synthesized thiacalix[4]arene as a tool in researching biochemical processes in mitochondria. A simulation model employing functional hybrid Petri nets was developed, reproducing the functional activity of mitochondria, including simultaneous NADH oxidation, ROS formation, NO synthesis, and Ca²⁺ accumulation. The derived equations formalize and describe the time dependencies of the listed processes in the medium under the influence of thiacalix[4]arene C-1193.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"69-81"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}