Biotechnologia最新文献

{"title":"Graphic analysis of various sulfur applications on safflower fatty acids profile.","authors":"Naser Sabaghnia, Mostafa Fattahi, Mohsen Janmohammadi, Amin Abbasi","doi":"10.5114/bta.2024.135640","DOIUrl":"https://doi.org/10.5114/bta.2024.135640","url":null,"abstract":"<p><p>In this study, we examined the effects of seven different sulfur treatments on safflower seeds. The treatments included: no sulfur application (S0), 25 kg/ha of pure bulk sulfur (S25), 50 kg/ha of pure bulk sulfur (S50), 25 kg/ha of sulfur phosphate (Sp25), 50 kg/ha of sulfur phosphate (Sp50), 25 kg/ha of zinc sulfate (Zs25), and 50 kg/ha of zinc sulfate (Zs50). Our evaluation covered various seed quality attributes, including ash percentage (ASH), oil percentage (OIL), and protein percentage (PRO). Additionally, we analyzed the fatty acid composition, including palmitic acid 16 : 0 (PAL), stearic acid 18 : 0 (STE), oleic acid 18 : 1 (OLE), linoleic acid 18 : 2 (LINL), arachidic acid 20 : 0 (ARA), and linolenic acid 18 : 3 (LINN). The vector-view of the biplot illustrated positive associations among the fatty acids STE, PAL, and OLE, whereas ASH exhibited negative associations with OIL, LINL, and LINN. The polygon-view graph was divided into four sectors, with the genotype S50 emerging as the top performer for attributes such as OIL, PRO, LINL, ARA, and LINN. Treatment Zs50 occupied the vertex of another sector and displayed the highest values for palmitic acid PAL, STE, and OLE, while treatment S0 was positioned at the vertex of the next sector, characterized by its high ASH content. By utilizing the ideal tester tool of treatment by trait biplot, we identified OIL as the desirable trait that most effectively represented the data. The qualitative properties of safflower oil were notably influenced by sulfur application, with treatment S50 proving to be the most effective in enhancing these properties.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"33-39"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the microbial diversity and proximate composition of smoked-fermented bushmeat from four different bushmeat samples.","authors":"Afia Sakyiwaa Amponsah, Gloria Mathanda Ankar-Brewoo, Herman Erick Lutterodt, Isaac Williams Ofosu","doi":"10.5114/bta.2024.135637","DOIUrl":"https://doi.org/10.5114/bta.2024.135637","url":null,"abstract":"<p><p>The ever-increasing demand for wildlife-derived raw or processed meat commonly known as bushmeat, has been identified as one of the critical factors driving the emergence of infectious diseases. This study focused on examining the bacterial community composition of smoked and fermented bushmeats, specifically grasscutter, rat, rabbit, and mona monkey. The analysis involved exploring 16Sr RNA amplicon sequences isolated from bushmeat using QIIME2. Microbiome profiles and their correlation with proximate components (PLS regression) were computed in STAMP and XLSTAT, respectively. Results indicate the predominance of <i>Firmicutes</i> (70.9%), <i>Actinobacteria</i> (18.58%), and <i>Proteobacteria</i> (9.12%) in bushmeat samples at the phylum level. <i>Staphylococcus</i>, <i>Arthrobacter</i>, <i>Macrococcus</i>, and <i>Proteus</i> constituted the core microbiomes in bushmeat samples, ranked in descending order. Notably, significant differences were observed between the bacterial communities of bushmeat obtained from omnivores and herbivores (rat and mona monkey, and grasscutter and mona monkey), as well as those with similar feeding habits (rat and monkey, and grasscutter and rabbit) at the family and genus levels. Each type of bushmeat possessed unique microbial diversity, with some proximate components such as fat in rat samples correlating with <i>Staphylococcus</i>, while proteins in Mona monkey correlated with <i>Arthrobacter</i> and <i>Brevibacterium</i>, respectively. The study underscores public health concerns and highlights probiotic benefits, as bushmeat samples contained both pathogenic and beneficial bacteria. Therefore, future research efforts could focus on improving bushmeat quality.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"5-17"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA.","authors":"Asha Anand, Shailja Chauhan, Aparna Chodon, Kavitha Velayudha Vimala Kumar, S Saravanakumar, Gopal Pandi","doi":"10.5114/bta.2024.135639","DOIUrl":"https://doi.org/10.5114/bta.2024.135639","url":null,"abstract":"<p><p>The microRNAs are endogenous, regulating gene expression either at the DNA or RNA level. Despite the availability of extensive studies on microRNA generation in plants, reports on their abundance, biogenesis, and consequent gene regulation in plant organelles remain naVve. Building on previous studies involving pre-miRNA sequencing in <i>Abelmoschus esculentus</i>, we demonstrated that three putative microRNAs were raised from the chloroplast genome. In the current study, we have characterized the genesis of these three microRNAs through a combination of bioinformatics and experimental approaches. The gene sequence for a miRNA, designated as AecpmiRNA1 (<i>A. esculentus</i> chloroplast miRNA), is potentially located in both the genomic DNA, i.e., nuclear and chloroplast genome. In contrast, the gene sequences for the other two miRNAs (AecpmiRNA2 and AecpmiRNA3) are exclusively present in the chloroplast genome. Target prediction revealed many potential mRNAs as targets for AecpmiRNAs. Further analysis using 5' RACE-PCR determined the AecpmiRNA3 binding and cleavage site at the photosystem II protein N (psbN). These results indicate that AecpmiRNAs are generated from the chloroplast genome, possessing the potential to regulate mRNAs arising from chloroplast gene(s). On the other side, the possibility of nuclear genome-derived mRNA regulation by AecpmiRNAs cannot be ruled out.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"105 1","pages":"19-32"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back to nature: henna extracts from nanotech to environmental biotechnology - a review.","authors":"Desouky A M Abd-El-Haleem","doi":"10.5114/bta.2023.132776","DOIUrl":"10.5114/bta.2023.132776","url":null,"abstract":"<p><p>The Lythraceae family includes henna (<i>Lawsonia inermis</i>), which thrives in subtropical and tropical climates. One of its many and long-standing uses is in cosmetics as a pigment to color hair and nails. Additionally, it serves as a disinfectant against microbiological infections and has traditional applications in the textile industry, specifically for coloring wool and nylon. The dried leaves of henna contain a significant amount of lawsone, an active substance bestowing them with staining abilities. Environmental biotechnology, a subfield of biotechnology, engages in the production of biomass or renewable energy sources and the elimination of pollutants, utilizing either entire organisms or their by-products. Recent research indicates that henna, owing to its sustainability, abundant production, simplicity of preparation, low cost, and reputation for being safe and ecologically benign, is exceptionally well-suited to participate in the realm of environmental biotechnology. This review navigates through the most recent studies exploring the use of henna and its extracts for related purposes. These encompass a spectrum of applications, including but not limited to nanobiotechnology, fabric dyeing, corrosion resistance, colored solar cells, carbon dots, and new renewable energy exemplified by biofuel and biohydrogen. Furthermore, henna extracts have been deployed to function as antimicrobials and ward off dangerous insects.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"421-434"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of <i>Crotalaria juncea</i> flower extracts as a source of antioxidants, antimicrobials, and cytoprotective agents for biomedical applications.","authors":"Pawika Mahasawat, Sawai Boukaew, Poonsuk Prasertsan","doi":"10.5114/bta.2023.132772","DOIUrl":"10.5114/bta.2023.132772","url":null,"abstract":"<p><p>Plants provide an unlimited source of bioactive compounds, possessing tremendous applications in the pharmaceutical industry. In the search for sources of antioxidants and antimicrobial agents against human pathogens, ethanol extracts of <i>Crotalaria juncea</i> flowers (CJ flower extract) were evaluated. The highest total phenolic (5.65 μg GAE/ml) and flavonoid (0.43 μg QE/ml) contents were observed in the 100 μg/ml CJ flower extract. To assess antioxidant activity, three <i>in vitro</i> antioxidant tests were employed: DPPH radical-scavenging, ABTS⁺ radical-scavenging, and hydroxyl radical-scavenging assay. The CJ flower extract demonstrated significant (<i>P</i> < 0.05) antioxidant activity, dependent on the percentage of solvent extraction and the specific assays utilized. The highest antioxidant activity was obtained with 100% ethanol extraction and using the hydroxyl radical-scavenging assay (56.63%). Antimicrobial activity was assessed against six human pathogens, including the fungi <i>Microsporum gypseum</i> and five Gram-positive bacteria (<i>Propionibacterium acnes</i>, <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, <i>Streptococcus pyogenes</i>, and <i>Streptococcus mutans</i>), as well as one Gram-negative bacterium (<i>Escherichia coli</i> ). The CJ flower extract inhibited the growth of both fungal and bacterial pathogens. The cytotoxicity of the CJ flower extract was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the highest concentration of the extract (100 μg/ml) did not affect L929 cell viability. Moreover, the CJ flower extract demonstrated the ability to suppress H₂O₂-induced toxicity in L929 cells. Overall, the CJ flower extract has potential as an alternative source for exploring new antioxidants, antimicrobial agents, and cytoprotectants that could prove valuable for biomedical applications.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"359-370"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting acid protease production by <i>Mucor circinelloides</i> MG603064.1 through SmF process: characterization and <i>fromage frais</i> making.","authors":"Souhila Bensmail, Khaled Boudjema, Fethia Naimi-Fazouane, Samira Bensmail, Djamila Djouahra-Fahem, Fatiha Ferhoum, Nassima Bourfis","doi":"10.5114/bta.2023.132770","DOIUrl":"10.5114/bta.2023.132770","url":null,"abstract":"<p><p>The exploitation of food industry wastes and their conversion into value-added products present a promising and continuously growing field, given the diversity of elaborated wastes. The current work aimed to utilize sweet cheese whey as a growth medium for acid protease production by a local fungus strain. The biochemical and physicochemical properties of the cheese whey, such as pH, conductivity, chemical oxygen demand, biological oxygen demand (BOD₅), total nitrogen and protein contents, and mineral salts, were assessed using various analytical methods. The effect of certain parameters on acid protease production by <i>Mucor circinelloides</i> MG603064.1 through the SmF process was investigated using the conventional design method \"One factor at a time\". Subsequent to characterization, the crude extract was used in a trial to create <i>fromage frais</i>, compared to the commercial rennin CHY-MAX^® Powder Extra. Cheese whey characterization revealed its richness in total nitrogen (1.044 ± 0.044 g/l), protein content (6.52 ± 0.04 g/l), and principal mineral salts: calcium (1.637 ± 0.037 g/l), phosphorus (1.173 ± 0.023 g/l), and chloride (1.66 ± 0.09 g/l). The optimal values of the SmF process for acid protease production, such as the inoculum size, beef extract, and KH₂PO₄ supplements, the initial pH of cheese whey, and incubation temperature were, respectively, 11% (v/v), 0.4% (w/v), 0.5% (w/v), 5.5, and 30°C. Under these conditions, the lowest milk-clotting time of 290 s was achieved, representing an 18.41-fold increase compared to the initial step using the unoptimized medium. The enzyme showed maximum milk-clotting activity at pH 5, a temperature of 60°C, and in the presence of 0.025 M of CaCl₂. The enzyme activity also significantly improved with sonication (35 kHz) for 10 min. The crude extract of <i>M. circinelloides</i> ensured the production of fresh cheese samples with characteristics roughly similar to those obtained by the control (CHY-MAX^® rennin). The acid protease of <i>M. circinelloides</i> could successfully substitute the conventional rennin in the manufacture of fresh cheese.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"333-349"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed germination dynamics of some woody legumes: implication for restoration of arid zones ecosystems.","authors":"Toma Buba, Abalis Gaya Ezra, Sunday Paul Bako, Mohammad Umar Sabo","doi":"10.5114/bta.2023.132774","DOIUrl":"10.5114/bta.2023.132774","url":null,"abstract":"<p><p>The seed germination dynamics of <i>Acacia nilotica</i>, <i>Bauhinia rufescens</i>, <i>Faidherbia albida</i>, and <i>Piliostigma reticulatum</i> were investigated over 28 days. Seeds were pretreated with concentrated sulfuric acid. Determined germination parameters included germination energy, germination period, germination capacity, germination inertia, and viability loss. Seeds exposed to sulfuric acid for extended periods (30, 40, 50, and 60 min) exhibited a higher germination rate (α = 0.05). For <i>A. nilotica</i> seeds, the 50 min acid treatment resulted in the highest germination energy of 85.5% and germination capacity of 89.5% (<i>P</i> = 0.001); conversely, the 60-min treatment yielded the highest germination energy and capacity, both 96.5% (<i>P</i> = 0.079), for <i>P. reticulatum</i>. In the case of <i>B. rufescens</i>, the 30-min treatment led to the highest germination energy of 93% and germination capacity of 88% (<i>P</i> = 0.001). For <i>F. albida</i>, all acid treatments resulted in 100% for both germination energy and germination capacity (<i>P</i> = 0.621). Viability losses for <i>A. nilotica</i> and <i>P. reticulatum</i> were higher (32 and 30%, respectively) than those for <i>B. rufescens</i> and <i>F. albida</i>, which were 19.5 and 6%, respectively (<i>P</i> = 0.000). Generally, higher germination energy resulted in lower viability loss, dependent on the species. Analyses of germination inertia and viability loss suggest that seeds of <i>A. nilotica</i> and <i>P. reticulatum</i> possess a greater ability to survive in arid land climates than those of <i>B. rufescens</i> and <i>F. albida</i>. However, due to the advantage of lower viability loss, <i>B. rufescens</i> and <i>F. albida</i> should be preferred for the natural restoration of arid land ecosystems where seed availability is a major concern.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"381-402"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> immune evaluation of adenoviral vector-based platform for infectious diseases.","authors":"Joanna Baran, Łukasz Kuryk, Teresa Szczepińska, Michał Łaźniewski, Mariangela Garofalo, Anna Mazurkiewicz-Pisarek, Diana Mikiewicz, Alina Mazurkiewicz, Maciej Trzaskowski, Magdalena Wieczorek, Katarzyna Pancer, Ewelina Hallmann, Lidia Brydak, Dariusz Plewczynski, Tomasz Ciach, Jolanta Mierzejewska, Monika Staniszewska","doi":"10.5114/bta.2023.132775","DOIUrl":"10.5114/bta.2023.132775","url":null,"abstract":"<p><p>New prophylactic vaccine platforms are imperative to combat respiratory infections. The efficacy of T and B memory cell-mediated protection, generated through the adenoviral vector, was tested to assess the effectiveness of the new adenoviral-based platforms for infectious diseases. A combination of adenovirus AdV1 (adjuvant), armed with costimulatory ligands (ICOSL and CD40L), and rRBD (antigen: recombinant nonglycosylated spike protein rRBD) was used to promote the differentiation of T and B lymphocytes. Adenovirus AdV2 (adjuvant), without ligands, in combination with rRBD, served as a control. <i>In vitro</i> T-cell responses to the AdV1+rRBD combination revealed that CD8+ platform-specific T-cells increased (37.2 ± 0.7% vs. 23.1 ± 2.1%), and T-cells acted against SARS-CoV-2 via CD8+TEMRA (50.0 ± 1.3% vs. 36.0 ± 3.2%). Memory B cells were induced after treatment with either AdV1+rRBD (84.1 ± 0.8% vs. 82.3 ± 0.4%) or rRBD (94.6 ± 0.3% vs. 82.3 ± 0.4%). Class-switching from IgM and IgD to isotype IgG following induction with rRBD+Ab was observed. RNA-seq profiling identified gene expression patterns related to T helper cell differentiation that protect against pathogens. The analysis determined signaling pathways controlling the induction of protective immunity, including the MAPK cascade, adipocytokine, cAMP, TNF, and Toll-like receptor signaling pathway. The AdV1+rRBD formulation induced IL-6, IL-8, and TNF. RNA-seq of the VERO E6 cell line showed differences in the apoptosis gene expression stimulated with the platforms vs. mock. In conclusion, AdV1+rRBD effectively generates T and B memory cell-mediated protection, presenting promising results in producing CD8+ platform-specific T cells and isotype-switched IgG memory B cells. The platform induces protective immunity by controlling the Th1, Th2, and Th17 cell differentiation gene expression patterns. Further studies are required to confirm its effectiveness.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"403-419"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential role of the <i>BRCA2B</i> gene in somatic homologous recombination in <i>Arabidopsis thaliana</i>.","authors":"Padinhatta Purayil Amritha, Jasmine M Shah","doi":"10.5114/bta.2023.132773","DOIUrl":"10.5114/bta.2023.132773","url":null,"abstract":"<p><p>Constant exposure to various environmental and endogenous stresses can cause structural DNA damage, resulting in genome instability. Higher eukaryotic cells deploy conserved DNA repair systems, which include various DNA repair pathways, to maintain genome stability. Homologous recombination (HR), one of these repair pathways, involves multiple proteins. BRCA2, one of the proteins in the HR pathway, is of substantial research interest in humans because it is an oncogene. However, the study of this gene is limited due to the lack of availability of homozygous <i>BRCA2</i>-knockout mutants in mammals, which results in embryonic lethality. <i>Arabidopsis thaliana</i> has two copies of the <i>BRCA2</i> homologs: <i>BRCA2A</i> and <i>BRCA2B</i> . Therefore, the single mutants remain nonlethal and fertile in <i>Arabidopsis</i>. The <i>BRCA2A</i> homolog, which plays a significant role in the HR pathway of germline cells and during the defense response, is well-studied in <i>Arabidopsis</i>. Our study focuses on the functional characterization of the <i>BRCA2B</i> homolog in the somatic cells of <i>Arabidopsis</i>, using the homozygous <i>ΔBRCA2B</i> mutant line. The phenotypic differences of <i>ΔBRCA2B</i> mutants were characterized and compared with wild <i>Arabidopsis</i> plants. The role of <i>BRCA2B</i> in spontaneous somatic HR (SHR) was studied using the <i>ΔBRCA2B</i>-gus detector line. <i>ΔBRCA2B</i> plants have a 6.3-fold lower SHR frequency than the control detector plants. Expression of four other HR pathway genes, including <i>BRE</i>, <i>BRCC36A</i>, <i>RAD50</i>, and <i>RAD54</i>, was significantly reduced in <i>ΔBRCA2B</i> mutants. Thus, our findings convey that the <i>BRCA2B</i> homolog plays an important role in maintaining spontaneous SHR rates and has a direct or indirect regulatory effect on the expression of other HR-related genes.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"371-380"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Prussian carp (<i>Carassius gibelio</i> B.) oocytes under the influence of Roundup^® herbicide.","authors":"Klaudia Kwiatkowska","doi":"10.5114/bta.2023.132771","DOIUrl":"10.5114/bta.2023.132771","url":null,"abstract":"<p><p>The aim of this study was to investigate the effect of Roundup^® herbicide on the maturation of Prussian carp oocytes under laboratory conditions. The Prussian carp is currently one of the most common fish species in Polish freshwater ichthyofauna. For the investigation, oocytes from five sexually mature female Prussian carp were used, segmented into three groups, and incubated for 24 h in Cortland's saline, treated with varied concentrations of the herbicide Roundup^® (0 ng - control, R1 - 10 ng/ml, and R2 - 100 ng/ml). Subsequent to this period, assays were performed using the prepared plates to determine the level of 17α,20β-dihydroxyprogesterone (17α,20β-P) utilizing the standard ELISA technique. In determining the 17α,20β-P via ELISA, the medium was extracted from each tested oocyte group. Oocyte maturity was assessed through preservation in serra fluid, and, to categorize the maturity stage of the oocytes utilizing a four-point scale - contingent upon the nucleus's position - the formerly preserved oocytes were dehydrated and subsequently analyzed. A contrast was noted in the percentage of oocytes at varied stages between the control group and the experimental groups. Specifically, a higher concentration of Roundup^® (100 ng/ml) accelerated to expedite the initial migration of the nucleus in oocytes. In conclusion, the obtained results show the adverse effect of Roundup^® on hormonal regulation and maturation in Prussian carp oocytes.</p>","PeriodicalId":94371,"journal":{"name":"Biotechnologia","volume":"104 4","pages":"351-358"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139428230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}